RNA Interference in Infectious Tropical Diseases

Trypanosomatids

RNAi in T. brucei has been studied extensively since the machinery was discovered in the organism [11]. T. brucei has been used to elucidate the functions of proteins comprising the RNAi machinery such as Ago1 and dicer [52-54], as well as basic RNAi mechanisms. Researchers constructed a stem-loop that generated hairpin-loop dsRNAs targeting the α-tubulin gene under the control of the tetracycline-inducible promoter. This system inhibited α-tubulin expression in T. brucei [55]. Wang et al. [56] used a vector (pZJM) in which a PCR amplified gene fragment was ligated between opposing promoters to inhibit specific gene expression in T. brucei. Therefore, sense and antisense RNAs were simultaneously synthesized and hybridized, generating dsRNAs molecules. The synthesis of dsRNA targeting α-tubulin transcripts was induced by tetracycline.

It was confirmed that T. congolense, which is the causative agent of nagana disease in cattle, also has RNAi machinery by utilizing a tetracycline-regulated vector expression system [57]. The constructed vector was transfected into T. congolense cells, inducing the same RNAi mechanisms as in T. brucei. The α-tubulin synthesis was reduced when the vector was transfected into a T. congolense cell line in the presence of tetracycline, demonstrating similar results to the previous experiment targeting α-tubulin gene in T. brucei. Induction of the RNAi pathway changed the morphology of transfected T. brucei cells after α-tubulin transcripts were silenced [11]. This suggested that RNAi machinery exists in T. congolense.

Conversely, T. cruzi, Leishmania donovani and L. major lack RNAi machinery although they belong to the same family as T. brucei that contains the necessary components of the RNAi pathway [58-60]. Upon searching genome databases, it was found that these species are deficient in orthologs of the Ago1 protein. It is not surprising that RNAi has not been observed without Ago1, since this protein is required for suppression of foreign and endogenous transgenes [32]. Another missing component is the PAZ domain, normally present in Ago. The presence of a Piwi domain is indicative of a functional RNAi mechanism because it is a part of the Ago protein that associates with siRNA to cleave target mRNA [61]. However, species that do not have an RNAi mechanism lack proteins with a PAZ domain [16], which is a subdomain of Ago and the dicer protein which binds to siRNA, contributing to firm incorporation of siRNA and miRNA into the RISC complex [62]. The absence of these domains may explain why certain species do not have an RNAi pathway.

Apicomplexa

Apicomplexa is characterized by its unique feature, an apical complex that projects to contact the host cell. The RNAi pathway has been researched in Plasmodium, but it is still controversial whether the RNAi pathway functions in Plasmodium or not. RNAi-like effects were first reported in P. falciparum [63]. An electroporation method was employed to investigate the role of dsRNA targeting dihydroorotate dehydrogenase (DHODH), an enzyme related in the pyrimidine biosynthesis pathway. Treatment with dsRNA resulted in reduction of DHODH transcript levels, causing an inhibition of parasite growth [63]. Another study providing evidence for the presence of RNAi was the reduction of a cysteine protease by dsRNA in P. falcipaum. The treated Plasmodium showed a typical cysteine protease deficient phenotype. Moreover, authors showed that the introduced dsRNA was processed into short 25 bp fragments. Therefore, this study seemed to provide clear evidence for the presence of RNAi machinery in Plasmodium [64].

However, Ullu et al. [16] disagreed that this study provided sufficient evidence to confirm the presence of RNAi in Plasmodium. They argued that McRobert and McConkey [63] did not perform a Northern analysis to confirm the data. Therefore, it was not proven that the RNAi-induced phenotype was not a non-specific RNAi effect. They also questioned if the siRNA found in Plasmodium [64] may have been broken dsRNAs of cysteine protease, resulting from contamination by the host cells [16]. The contamination hypothesis was further supported by a subsequent paper [65], in which short RNAs were searched to confirm that siRNA exists in P. falciparum and eventually to provide evidence for the RNAi pathway in Plasmodium. However, the authors did not find any short RNAs and discovered miRNAs that turned out to be human miRNA, suggesting the results by Malhotra et al. [64] might have been false positives [65]. Central evidence supporting the absence of RNAi pathway in Plasmodium is based on the lack of canonical proteins and protein domains such as dicer, Piwi, PAZ, or RdRp that are found in the RNAi mechanism [16].

Database mining has hinted at the presence of classical RNAi genes in Toxoplasma gondii, suggesting that RNAi could potentially exist in Toxoplasma [65]. A recent report suggested the existence of RNAi pathway in T. gondii when the introduction of dsRNA targeting uracil phosphoribosyltransferase reduced endogenous target RNA transcript levels, and this result was confirmed by Northern analysis [66]. However, there was no evidence of siRNA inducing RNAi in the treated T. gondii, which makes the presence of an RNAi pathway in T. gondii doubtful [16]. Some researchers argue that antisense oligonucleotides (ASO) can reduce target mRNAs in the absence of RNAi in protozoans [67]. In fact, Plasmodium genes such as cytoadherence-linked gene and glucose-6-phosphate dehydrogenase gene were silenced by their antisense RNAs, suggesting that antisense RNAs can reduce their target gene transcripts levels in the absence of RNAi machinery [68,69]. ASO may also explain the reduction of target transcripts in T. gondii.

Other protozoans

In order to search for proteins crucial to the RNAi pathway in a specific species, database mining is quite informative. Structural proteome databases were searched and a dicer protein was identified in Giardia intestinalis [70]. A model was constructed in which dicer processed dsRNA into short RNAs and simulated the RNAi pathway in Giardia. Characterization of RNase III and Ago2, hallmarks of the RNAi pathway, led to the prediction that RNAi was present in Entamoeba histolytica [71]. Screening for the presence of siRNA in E. histolytica is an alternative method to check for the existence of an RNAi pathway. Ullu et al. [72] identified a novel class of sense and antisense RNAs homologous to a retroposon family, GilT / Genie1 in Giardia. The sense and antisense RNAs were 20 to 30 nucleotide-long and fragmented, suggesting they may inhibit the retroposon via an RNAi pathway. This prediction should be experimentally confirmed prior to practical utilization of the RNAi pathway in Giardia.

Prospects of RNAi in parasitic protozoans

It is important to first identify RNAi genes in protozoan species because many protozoans may lack the genes responsible for the RNAi pathway. Once the presence of the RNAi molecules is confirmed, RNAi can be employed to elucidate gene function or to hinder parasite development for control purposes. RNAi can be utilized by a vector system, which would generate dsRNAs under the control of an inducible promoter or with tissue-specific expression. A vector system has been studied in T. brucei and can be applied to different parasitic protozoan species to achieve temporal and spatial induction of RNAi. Some researchers described the role of antisense RNA in knocking down target mRNA in Plasmodium. Although this is not a conventional RNAi pathway, it should be further exploited to use the antisense-knockdown system to silence specific genes [73].

Nematodes

Nippostrongylus brasiliensis [75], a rat intestinal parasite, was the first nematode for which RNAi effects were reported. This parasite is an important animal model since it shares a similar life cycle with the human hookworms, Necator americanus and Ancylostoma duodenale. When 1,799 bp-long dsRNAs targeting full length of acetylcholinesterases (AchEs) cDNA at the concentration of 1 mg / ml dsRNA were utilized, the target gene was suppressed nearly by 80% on the first day, but then the transcripts returned to normal levels in 4 days. By targeting AchEs with 240 bp of dsRNAs, AchEs were suppressed by more than 90% and the effects lasted for 6 days, suggesting that the short dsRNA were effective in suppression of target gene expression. This experiment provided the first evidence of a successful RNAi effect in a parasitic helminth. Therefore, control of helminths via RNAi seems to be feasible once a suitable target gene is identified.

RNAi effects in Brugia malayi suggested a more promising control method while targeting housekeeping genes (β-tubulin and RNA polymerase II large subunit) [76]. This study showed that 300 bp long dsRNA was effective enough to result in death of the filarial worm. The authors also utilized RNAi to target another gene, microfilaria sheath protein 1 / mf22, but this was not lethal to the worms although microfilariae release was reduced and half of the released microfilariae did not have fully elongated sheathes. B. malayi is an important lymphatic filarial nematode and it is difficult to block their transmission by mosquitoes. Therefore, the lethal effect from RNAi was significant as it proved to be a potential control system for B. malayi.

Although previous studies reported a high concentration of dsRNA was needed to knock down target genes in various helminths [75-77]. Pfarr et al. [78] claimed that low concentration of dsRNA was enough to specifically knock down a target gene in Litomosoides sigmodontis, a rodent filaria. All concentrations ranging from 0.035 to 35 µM of dsRNA comparably reduced actin gene transcripts in adult worms by more than 90%. They also measured induction of hsp60 gene to figure out any stressful response on dsRNA injection. Of these concentrations, 3.5 µM of dsRNAs reduced the target with the least variation and no hsp60 induction, but high concentrations (17.5 and 35 µM) resulted in a significant increase in hsp60 transcripts, indicating stress in the filarial worm by high dsRNA dosage. This study suggests that low concentrations are enough to reduce transcript levels consistently whereas high concentrations of dsRNA may be stressful to the filarial worms. Thus, titration of appropriate concentration of dsRNAs would be required prior to RNAi experiments.

Functional RNAi-knockdown was reported in L3 larvae of Onchocerca volvulus. Lustigman et al. [79] targeted cathepsin L and cathepsin Z-like cysteine proteases that play important roles in L3 to L4 larvae for molting. Soaking the third-stage larvae (L3) in a dsRNA solution reduced the molting rate by 92% for cathepsin L and 86% for cathepsin Z-like cysteine proteases. Gene silencing of these cathepsin transcripts delayed the molting process by 1-3 days, resulting in significant reduction in the viability of the L3 larvae in O. volvulus [80]. Gene silencing of inorganic pyrophosphatase of the parasitic round worm, Ascaris suum, inhibited molting from L3 to L4 by 31% [81]. Although the inhibition rate was lower than that of O. volvulus, discovery of functional gene silencing by RNAi in Ascaris worms was significant and this can be further exploited as a model system to study RNAi in human ascariasis.

Issa et al. [82] suggested that siRNA and electroporation are more efficient molecules and a delivery method, respectively, to induce gene silencing by RNAi in the sheep gastrointestinal parasite, Trichostrongylus colubriformis. The authors tested three different RNAi delivery methods; feeding of Escherichia coli expressing dsRNA, soaking of siRNA or dsRNA, and electroporation of siRNA or dsRNA. Ubiquitin and tropomyosin were used as target genes since their DNA sequences are well conserved and readily available. Ubiquitin transcripts were not reduced by the E. coli feeding method, but tropomyosin was suppressed. siRNA in both electroporation and soaking resulted in a significant reduction for both target genes. This study demonstrated that electroporation is likely to result in more consistent gene silencing, and that 22 bp siRNA was more effective than long dsRNA in reducing expression of both target genes.

A variety of RNAi conditions were tested to optimize RNAi effects in Haemonchus contortus, a barberpole worm. Various life stages (L1- L4 and adult), 11 genes (β-tubulin, sec-23, Ca²⁺ binding protien, HSP70, vacuolar ATPase, cathepsinL, paramyosin, Cu-Zn superoxide dismutase, intermediate filament, type IV collage and GATA transcription factor) and 3 different RNAi delivery methods (feeding, soaking and electroporation) were tested in this organism to investigate RNAi [83,84]. Two β-tubulin genes that were targeted by RNAi affected 3 life stages (L3, L4 and adult) and reduced target gene transcripts by the soaking method, however reduced motility and viability were only shown in the L3 stage [84]. Geldhof et al. [83] tested RNAi effects on 11 different genes of the L1-L3 stages by 3 different delivery methods in H. contortus. The feeding method was not effective in reducing target gene transcripts, confirming previous data in Trichostrongylus [82]. Only two transcripts (β-tubulin and sec-23) out of 11 genes in the L3 stage were significantly reduced by soaking in dsRNA. Interestingly, no phenotypic change was observed in the L3 larvae soaked in siRNA, and some control siRNA was even toxic to the L1 / L2 larvae. Electroporation was effective in reducing target gene transcripts in L1 larvae, as transcript levels of β-tubulin and superoxide dismutase were significantly decreased. However, larval death was observed in the L1 / L2 stage by electroporation even in the presence of control dsRNA, suggesting either electroporation is not a stable delivery method to this stage or current electroporation protocols for this stage are not optimal for analyzing RNAi effects.

Similarly, eight genes were tested in the L1 and L3 larval stages of Ostertagia ostertagi, a cattle parasitic nematode, by electroporation and soaking delivery methods. Significant reduction of transcripts were observed for five target genes (tropomyosin, β-tubulin, ATPase, superoxide dismutase and a polyprotein allergen) in L3 larvae, but dsRNAs of a transthyretin-like protein, a 17 kDa ES protein and ubiquitin did not reduce the target gene transcript levels. Electroporation was less effective, as only two genes (tropomyosin and β-tubulin) were successfully silenced, and these RNAi effects were not even reproducible [85]. This non-reproducible result indicated the RNAi delivery method will require further optimization to achieve consistent results.

Trematodes

RNAi research in trematodes has been mainly focused on Schistosoma mansoni, blood flukes, which cause hepatosplenomegaly, hematemesis or liver failure, and may result in mortality. The first report of RNAi in Schistosoma targeted the cathepsin B gene [86], an enzyme previously proposed to be responsible for degradation of host hemoglobin to digestible peptides [87]. Shistosomes were soaked in dsRNA targeting cathepsin B and cultured for six days. The authors confirmed by RT-PCR that parasites soaked in the dsRNAs showed reduced target gene expression. Subsequent studies suggested electroporation as an alternative way to introduce dsRNA, and reduction of transcript levels of cathepsin B in schistosomula was demonstrated. Later RNAi research proved that cathepsin B is essential for parasite growth, and not essential for hemoglobin digestion [88].

RNAi was used to test another gene function in the sporocyst developmental stage of schistosomes. Boyle et al. [89] knocked down SGTP1, a facilitated diffusion glucose transporter and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by RNAi. The expression of both genes in the sporocyst stage was reduced when miracidia, a free living-larval stage of schistosome were soaked in its homologous dsRNA and allowed to transform to the sporocyst stage. Glucose transport activity was reduced when SGTP1 was knocked down, demonstrating the function of the gene in schistosome. Interestingly, however, when dsRNAs were introduced at the sporocyst stage, the reduction was not observed, suggesting RNAi is not effective when introduced into this developmental stage. The effect of RNAi lasted for 28 days, which is similar to C. elegans [90]. RNAi can, therefore, be introduced at the miracidia stage and the effects will last well into the sporocyst stage.

RNAi was utilized to reduce transcripts of scavenger receptors that are known to be important for the binding of low-density lipoprotein to the surface of schistosomula and adult S. mansoni. Because the parasites cannot synthesize sterols and fatty acids, uptake of host-lipoprotein by scavenger receptors is critical in the synthesis of biological membranes. Miracidia were soaked in dsRNA targeting the scavenger receptors for six days and this resulted in a 60-70% reduction of the target gene transcript level. Reduction of the scavenger receptors inhibited normal parasite development, showing a more rounded morphology in sporocysts and a shorter length in larval size [91].

RNAi was also used to target the gynecophoral canal protein SjGCP, in Schistosoma japonicum, a major human pathogen in the Far East [77]. Schistosomes cannot develop without correct signaling during sexual pairing [92], so disruption of pairing would be good target in order to control schistosomes. It is known that S. mansoni GCP, an ortholog of SjGCP, is related to pairing and produced only by males [93,94]. Cheng et al. [77] utilized RNAi to show that the SjGCP can be silenced in a dosage-dependent manner in S. japonicum. The target gene transcripts were reduced by 75% with 100 nM of dsRNA, but not affected at 12.5 nM. This study suggested a potential therapeutic application of RNAi in S. japonicum. With reduced SjGCP, the mating in S. japonicum may be disturbed, which will cause inhibition of parasite development in the host.

Current obstacles and future prospects of RNAi in helminths

While genes are successfully silenced by RNAi in C. elegans, gene knockdown by RNAi has been either impossible or inconsistent in other helminths [74]. For example, Geldhof et al. [83] tested efficiency of RNAi silencing in the strongylid parasitic nematode, H. contortus, using three different dsRNA delivery methods- feeding, soaking, or electroporation. In this study, no RNAi was observed with dsRNA feeding among four genes tested and only two genes out of eleven, β-tubulin and sec-23, showed specific gene silencing by the soaking method. Similarly, two genes out of four had knockdown of target transcripts by electroporation of respective dsRNAs. Moreover, extents of gene silencing by RNAi also varied in the cattle parasitic helminth, O. ostertagi and the results were often difficult to reproduce [85]. Disagreement of results of RNAi in parasitic helminths may be due to different delivery methods employed for introducing dsRNAs into helminths [83]. There appears to be no consensus as to which is the most efficient delivery method to induce RNAi gene silencing in parasitic helminths Several reports have indicated electroporation was an efficient alternative to the soaking method in delivering dsRNA in T. colubriformis and schistosomes [82,88,95]. In contrast, others suggested that electroporation was not effective in O. ostertagi and in fact lethal to certain stages of H. contortus [83,85]. Feeding dsRNA is largely inefficient in reducing transcript levels of target genes except for tropomyosin in T. colubriformis [82]. Soaking is another popular method for gene silencing, but this method was less efficient for the L1 / L2 stage of H. contortus [83] and for S. mansoni [95]. Apparently, all major methods commonly used for RNAi in C. elegans have their limitations in the delivery of dsRNA into parasitic helminths.

The disagreement in RNAi efficiencies among parasitic helminths may result from the absence of sid (systemic RNA interference-deficient)-1, sid-2 or rsd (RNAi spreading defective)-4 in some of parasitic helminths. SID-1, SID-2, and RSD-4 proteins are involved in cellular uptake and spread of dsRNA in C. elegans [96]. SID-1 is a transmembrane protein that is required for RNAi uptake into cells and spread between cells. Therefore, the absence of these proteins in certain helminths can prevent externally-provided dsRNA from entering cells, making worms refractory to RNAi. In sid-1 mutant C. elegans, systemic spread of siRNA molecules was defective with any dsRNA delivery methods (feeding, soaking and microinjection) [97]. SID-2 is also a transmembrane protein, of which expression is limited in the apical membrane of the intestinal lumen. Therefore, SID-2 is believed to be required for dsRNA uptake from the lumen to cells but not for spreading siRNA between cells. In sid-2 deficient C. elegans resistant to RNAi by soaking and feeding methods, dsRNA delivered to the pseudocoelom (body cavity) by microinjection could initiate RNAi and siRNA were autonomously spread into cells [98]. As a result, sid-2 deficient C. elegans became susceptible to RNAi, showing reduction of target transcripts. Likewise, rsd-4 mutant C. elegans resistant to RNAi by feeding was reverted to be susceptible to RNAi by supplying external dsRNA into the pseudocoelom using microinjection [99]. Therefore, it seems there is functional overlapping between sid-2 and rsd-4 proteins for systemic spread of siRNA.

Many parasitic animal nematodes are deficient of sid-1, sid-2, or rsd-4 orthologs, or their homologs do not share the same functions with their counterparts in C. elegans [96,98,99]. The lack of sequence or functional conservation of sid-1, sid-2, or rsd-4 may account for the discrepant RNAi results between C. elegans and animal parasitic nematodes. To contradict this explanation, despite the fact that there is no identifiable sid-1 ortholog in B. malayi, a functional RNAi mechanism was observed by a soaking method [76]. This suggests that there may be alternative molecules or pathways substituting sid-1 for systemic spread of siRNAs in B. malayi. Therefore, it remains to be seen how systemic RNAi was achieved without sid-1 orthologs in B. malayi.

To achieve gene-specific phenotypic knock-downs for functional studies and therapeutic applications, certain modifications or improvements for the RNAi delivery methods may be required in animal parasitic helminths. To this end, two approaches have been recently suggested [96]. Firstly, one may be able to trigger RNAi by microinjection of dsRNA into the pseudocoelom of parasitic helminths if failure of RNAi by soaking or feeding is due to the absence of sid-2 orthologs in the gut lumen. This modification is based on the observation in Caenorhabditis briggsae, in which unlike C. elegans there is no sid-2 ortholog functionally conserved to allow entry of dsRNA from the gut lumen to the body cavity. Therefore, RNAi cannot be initiated in C. briggsae by dsRNA using soaking and feeding methods. However, RNAi was possible when dsRNA was supplied to the pseudocoelom via microinjection, suggesting that microinjection can bypass barriers of dsRNA uptake in the lumen, making RNAi possible in C. briggsae [98,100]. Secondly, RNAi may be feasible in sid-1 or 2-deficient parasitic helminths by heterologous expression of sid-1 and / or sid-2 of C. elegans as shown by Winston et al. [98]. In C. briggsae, for example, there is no functionally conserved sid-2 and RNAi by soaking or feeding is thus impossible. However, transformation of C. briggsae with C. elegans sid-2 allowed the uptake of dsRNA from the lumen in soaking experiments, generating systemic RNAi in C. briggsae [98]. As transgenesis of helminths becomes available, development of an RNAi system through heterologous expression of C. elegans genes for soaking or feeding may have promising applications for gene functional studies and target identification for drug discovery (Fig. 2).

Fig. 2

Functions of SID-1 and SID-2 in systemic RNAi in C. elegans. (A) SID-1 as a channel, allowing dsRNAs to diffuse into and between cells. sid-1 mutant C. elegans or sid-1-deficient worms neither can take up dsRNAs from environment nor can spread between cells. Therefore, both microinjection and feeding methods are not effective to deliver dsRNAs. This RNAi-deficiency can be rescued by heterologous expression of C. elegans wild type sid-1 [96,97]. (B) SID-2 also acts as a channel for dsRNAs but is only localized in the apical intestinal lumen. Thus, SID-2 is responsible for dsRNA uptake from environment (e.g. from lumen to pseudocoelom), but not for spread between cells. Feeding dsRNA is not effective for gene silencing in sid-2 mutant C. elegans or sid-2-deficient worms, but microinjection can be used for dsRNA delivery because dsRNAs in the pseudocoelom can be spread systemically via SID-1. sid-2 mutants can be rescued for RNAi by heterologous expression of a wild copy of C. elegans sid-2 [96,98].

RNAi screen is a less demanding method for drug target studies in helminths compared to forward genetic research [15]. If, however, RNAi machinery requires complicated dsRNA delivery process in animal parasitic nematodes, C. elegans can be served as a model system to study gene functions. Since RNAi machinery is well characterized in C. elegans, this experimental system can provide a potent tool for unveiling gene functions in other nematodes via comparative genomics [101]. The feasibility of this approach has been recently demonstrated in B. malayi [102]. Using sequence analyses and comparisons including gene functional studies with RNAi knockdowns in C. elegans, 589 B. malayi genes were identified as being critical to the survival of the filarial worm, representing potential targets for antifilarial drug discovery. Interestingly, among those 589 genes, 10 out of top 40 candidates were already previously known to be promising targets for the drug discovery because of their roles in molting, central metabolism, and structural components. This method may be realistic only for animal parasitic nematodes whose genomes are fully sequenced such as B. malayi [103]. Thus, genome sequencing of important helminths should be a prerequisite for this model animal based drug target studies.

RNAi in model insect species

RNAi has been used to silence target genes or analyze gene functions in many insect species. RNAi induced by dsRNA was shown in Drosophila both in vivo and in vitro [9,13]. By abolishing engrailed (en) transcripts with RNAi, Marie et al. [104] studied additional functions of the en gene that was previously known to control topography of axonal projections in D. melanogaster. Using larvae injected with en dsRNA, a change in axonal branching and synaptic outputs was found, thus demonstrating en controls synaptic choice as well as axonal projections.

After successful trials of RNAi in Drosophila, the RNAi technique was applied to other insect species. Quan et al. [12] injected dsRNA targeting the silkworm white gene (Bmwh3). Eggs of the wild type silkworm turn dark brown after oviposition. However, mutant strains of Bmwh3 show different patterns, with the eggs turning light brown, or remaining white. dsRNA targeting Bmwh3 inhibited expression of Bmwh3, resulting in white color or a mosaic pattern of egg color. The higher the concentration of dsRNA injected, the higher frequency of white and mosaic egg production. These results support the conclusion that RNAi could be applied to lepidopteran insects.

Application of RNAi for vector control

RNAi exists in several mosquito species, many of which are important disease vectors. When a premembrane coding region of the dengue virus type 2 genome was expressed in C6 / 36 cells derived from Aedes albopictus in sense and antisense orientation, the titer of specific type 2 virus was decreased, suggesting that dsRNA can induce resistance to virus infection in mosquito cells [105]. The same resistance pathway against dengue virus type 2 was shown when the premembrane coding region of the virus was expressed in Ae. aegypti adult mosquitoes [106]. These experiments clearly demonstrated the existence of RNAi in Aedes species and suggested the possibility of the use of RNAi to control vector-borne diseases. An. gambiae, an important vector species for malaria, also has the RNAi pathway [107]. Injection of dsRNAs targeting the endogenous Defensin gene, an antimicrobacterial peptide gene, silenced the Defensin transcripts, which increased the activity of gram-positive bacteria in An. gambiae. This showed the possibility of RNAi in elucidating gene functions in anopheline mosquitoes.

Analysis of gene functions using RNAi was employed to identify antiplasmodial genes in An. gambiae [108]. Microarray analysis was performed in mosquitoes infected by P. falciparum or P. berghei, and 11 candidate immune genes (Tep1, AgMDL1, FBN8, FBN9, FBN39, SPCLIP1, APOD, IRSP1, IRSP5, LRRD7, and gambicin) were identified, and the functions of these genes were assessed by RNAi gene silencing to show their antiplasmodium activities. dsRNAs of the 11 target genes were injected into mosquitoes and gene silencing was confirmed by real-time RT-PCR. Silencing of the each immune gene increased Plasmodium levels. In addition, RNAi was used to study antiviral effects of a heat shock cognate protein (HSC70B) in An. gambiae [109]. These reports showed RNAi can be used to analyze gene functions in An. gambiae.

The successful use of RNAi was extended to another important vector species. The cell line ISE6 from the Ixodes scapularis tick, which is a vector of Lyme disease and babesiosis, was infected by Hazara virus (HAZV) [110]. In this study, recombinant Semliki Forest virus (SFV) carrying HAZV gene in sense and antisense orientation was used. The expression of the dsRNAs of the recombinant virus efficiently inhibited HAZV replication, as monitored by western blotting and immunofluorescence assay. This suggests that RNAi can be used to control viral diseases and other infectious diseases in I. scapularis.

RNAi was recently used to examine the role of a specific gene in tsetse flies [111]. Tsetse flies, Glossina spp., transmit African trypanosomes (Trypanosoma brucei spp.), but the prevalence is low because the flies are refractory to trypanosome infection. Researchers compared the transcription levels of an antimicrobial peptide gene attacin, between refractory G. palpalis palpalis and susceptible G. morsitans morsitans. The levels of attacin expression were higher in the refractory species, suggesting that attacin may be involved in the refractoriness of G. palpalis palpalis. Subsequently, microinjection of dsRNAs targeting attacin was carried out in order to elucidate the role of the gene. When the attacin was silenced by dsRNAs, the infection rate significantly increased from 20% to 40%. This study showed that attacin is a refractory gene against African trypanosomes and also demonstrated that RNAi can be used as a powerful tool to investigate gene functions in tsetse flies.

Heritable and inducible RNAi effects in insects

Injection of dsRNA in insects can induce silencing effects rapidly, but the interference is transient and gene expression in later developmental stages may not be affected. Gene suppression by RNAi is also not inheritable when dsRNAs are traditionally prepared and delivered to organisms of study. However, stable and inheritable RNAi suppression was recently developed by generating transgenic insects expressing gene-specific dsRNAs. Researchers constructed a vector-based RNAi system in various organisms, which allowed in vivo RNAi that was heritable and inducible.

A heritable RNAi system was first tested in Drosophila, when hairpin dsRNA was prepared in vitro and injected into Drosophila embryos carrying the engrailed-lacZ gene. The results demonstrated significant inhibition of engrailed-lacZ gene expression, showing that the hairpin loop dsRNAs were as efficient as linear dsRNAs that are several hundreds bp-long. In subsequent studies, the researchers constructed transgenes that produced hairpin dsRNA in vivo. Transcription of the engrailed-lacZ gene was controlled by the Gal4 / UAS system. The Gal4 / UAS-IRlacZ produced hairpin-loop dsRNA and inhibited lacZ gene expression in vivo [112]. This system produced efficient dsRNAs to silence target genes. However, the inverted repeat construct for the hairpin formation was difficult to clone and unstable, so an improved method to generate dsRNA was required.

Giordano et al. [113] used a Gal4 / UAS system to generate both sense and antisense RNAs simultaneously in Drosophila to overcome the drawbacks of an inverted repeat construct. Three types of plasmids were constructed using a pUAST vector. First, Sym-pUAST-w was prepared, generating dsRNA by symmetric transcription using two identical promoters (UAS) flanking the target sequence (Drosophila white [w] gene) in opposite directions (head-to-head) (Fig. 3A). Then, 2 constructs, pUAST-IR_SP-w (Fig. 3B) and pUAST-IR-w (Fig. 3C) were prepared to compare with Sym-pUAST-w. Both pUAST-IR_SP-w and pUAST-IR-w contained an inverted repeat of the w gene, and generated hairpin dsRNAs. pUAST-IR_SP-w had a spacer between the inverted repeats while pUAST-IR-w lacked the spacer. Three independently transformed lines of Drosophila carrying the Sym-pUAST-w transgene were obtained and they were mated with the Act5C-Gal4 strain to generate hairpin dsRNA of the w gene. The progeny had light yellow eyes, exhibiting strong silencing of the w gene expression while the wild type had typical red eyes. The strains carrying pUAST-IR_SP-w also showed the light yellow eyes demonstrating reduction of w expression. The progeny carrying pUAST-IR-w showed heterogeneous expression at the phenotypic level with variegated eye color, suggesting the hairpin dsRNAs without a spacer were not efficient in silencing the target gene. This may be due to deletion in the center of inverted repeats, rendering the hairpin structure unstable. It is thus inferred that symmetrically transcribed transgenes efficiently triggered RNAi, and the system may be effective enough to replace the inverted repeat hairpin RNAi system.

Fig. 3

Strategies for generation of dsRNA in vivo by symmetric transcription. (A) SympUAST-w produces dsRNAs of the w gene by simultaneous transcription using two identical UAS promoters flanking the target gene in opposite directions. The target gene is transcribed in both directions and the resulting sense and antisense RNAs are hybridized to form dsRNAs. (B) pUAST-IRsp-w contains inverted repeats of the w gene with a spacer between the repeats. This is a common approach to generate hairpin dsRNAs. (C) pUAST-IR-w contains inverted repeats of the w gene without a spacer. This could generate hairpin dsRNAs, but the dsRNAs were not efficient enough to silence a target gene. This may be due to deletions in the center of inverted repeats, rendering the hairpin structure unstable. it appears that symmetrically transcribed dsRNA system may be effective enough to replace the inverted repeat hairpin RNAi system [113].

Prospects of RNAi in vector-borne diseases

RNAi can be employed to test a novel method for the control of vector-borne diseases. Malaria control using germline transformation and RNAi has been suggested [114]. Malaria is prevalent in sub-Saharan Africa and Southeast Asia where resources are limited for disease treatments and vector control.

Historically, chemical agents were the major means to control this mosquito-borne disease. However, resistance has become prevalent among mosquitoes and parasites against insecticides and anti-malarials, respectively [115-119]. Therefore alternative methods to control malaria disease and transmission are in dire need. A novel method to prevent malaria transmission by anopheline mosquito vectors is attractive. Mosquito genes such as leucine rich-repeat immune gene (LRIM1), C-type lectin (CTL4) and mannose binding CTL (CTLMA2) have been identified as candidates for malaria intervention since they are critical for parasite development. Silencing these candidate genes by RNAi resulted in impaired development of Plasmodium ookinetes to oocysts in mosquitoes, decreasing vector competence [114]. Therefore, this holds a potential for RNAi to be used as a novel malaria control method.

Vector control by RNAi can be applied to other vector species such as tsetse flies or ticks. RNAi was used in tsetse flies to identify a refractory gene against African trypanosomes [111], and it successfully silenced transgenes in the transfected tick cell line, ISE6, derived from I. scapularis [120]. These studies demonstrated the utility of RNAi in the study of gene functions in vector-pathogen interactions. With the identification of the tick genes that are responsible for pathogen development, RNAi can be employed to silence those genes and study their impact on pathogen development.

For gene-specific silencing, off-target effects must be considered as these non-specific effects are frequently reported in mammals [121]. A common consequence of off-target effects in mammals is an activation of interferon dependent responses [122,123]. However, insects do not share the same immune pathway. Thus, typical mammalian off-target effects may not cause concerns in insect vectors. Other common examples of off-target effects of RNAi may be over-dose lethality and toxic effects [74]. Soaking and electroporation are the main RNAi delivery systems in protozoans and helminths, which may cause general toxic effects due to long exposure time to dsRNA or injury by electroshock. Unlike parasitic pathogens, insect RNAi delivery relies on mostly microinjection. Thus, off-target effects caused by injection of non-physiological amounts of dsRNAs may be preventable by careful titration of dsRNAs to be used for RNAi experiments in insects.