Descriptions of Two Digenean Trematodes Found from a Chinese Sea Snake, Laticauda semifasciata, in Republic of Korea

Article information

Korean J Parasito. 2020;58(3):279-285
Publication date (electronic) : 2020 June 26
doi :
1Department of Parasitology, School of Medicine and Parasite Research Center, Chungbuk National University, Cheongju 28644, Korea
2National Marine Biodiversity Institute of Korea, Seocheon 33662, Korea
3National Institute of Ecology, Seocheon 33657, Korea
*Corresponding author: (
Received 2019 December 6; Revised 2020 May 14; Accepted 2020 May 19.


We intended to describe 2 digenean trematodes found from a Chinese sea snake, Laticauda semifasciata, as the new fauna in the Republic of Korea. The snake was caught offshore of Aewol-eup, Jeju-do, in August 2017. Two species of fluke were found in the lung and intestinal tract of the snake in the process of necropsy. They were identified as Pulmovermis cyanovitellosus Coil and Kuntz, 1960 and Harmotrema laticaudae Yamaguti, 1933, respectively based on the morphological characters. Pulmovermis cyanovitellosus showed elongated body with well-developed and elongated male genital system and compact vitelline. And H. laticaudae was characterized by linguiform body with heavily armed cirrus with excretory system. This is the first time both species have been reported and described off the Korean coast. We provide morphological descriptions with some comments on their biology and geographical distribution. In addition, the taxonomic validity of the genus Hydrophitrema Sandars, 1960 was discussed, in terms of morphological descriptions and host ranges. This study provides novel insight into digenean fluke species existing off the coast of Korea.


Sea snakes, also referred to as marine snakes, are a group of snakes that are fully adapted to underwater life and live in marine environments. They have flattened, paddle-like tails with advanced diving ability and/or have laterally compressed bodies adapted for their aquatic life. Generally, sea snakes are classified into 2 subfamilies called Hydrophiinae (true sea snakes) and Laticaudinae (sea kraits). They are widely recognized as having diverged and evolved from terrestrial elapid snakes [15].

Laticauda semifasciata (Reptila: Elapidae), also known as Chinese sea snake or black-banded sea krait, is a species that has which recently been added to the Korean herpetofauna list [6]. In the past, Chinese sea snake has been known to inhabit the tropical and subtropical regions of China, Japan, Philippines, and Taiwan [7]. However, recent studies have shown that the snakes are increasingly found outside their original habitats to the north, including the Republic of Korea (Korea), Russia, and the mainland of Japan [6,810].

The digenean flukes of L. semifasciata have previously only been investigated in their original habitats. Coil and Kuntz [11] 1960 reported 2 species of digenean flukes, i.e., Ateuchocephala marinus Coil & Kuntz, 1960 and Pulmovermis cyanovitellosus Coil & Kuntz, 1960, from Orchid Island (Lan Yu Isl.), Taiwan. In 1967, Telford examined 51 L. semifasciata specimens obtained from Amami Island, Japan, and found 2 distinct species of digenean parasites, namely P. cyanovitellosus and Harmotrema laticaudae Yamaguti, 1933 [12]. More recently, Ohashi et al. [13] recorded P. cyanovitellosus from Kudakajima Island, Japan. However, the digenean flukes of L. semifasciata in Korea have not been investigated to date.

Recently, we had the opportunity to examine a Chinese sea snake, L. semifasciata, collected from Korean waters, offshore of Jeju Island, and found 2 species of digenean flukes, H. laticaudae Yamaguti, 1933 (Digenea: Liolopidae) and P. cyanovitellosus Coil and Kuntz, 1960 (Digenea: Hemiuridae) for the first time in Korea. The aim of present study is to provide morphological descriptions with taxonomical and ecological comments on the 2 newly recorded species in Korea.


A Chinese sea snake, L. semifasciata was offered for necropsy to a veterinary clinic at the National Institute of Ecology, Seocheon, Korea on December 11, 2017. The snake was initially caught and donated by local fisherman to the National Marine Biodiversity Institute of Korea (MABIK) for sea snake research, but died while rearing in captivity. It was caught offshore of Aewol-eup, Jeju-do, Korea (33°28′38″N, 126°18′46″E) on August 11, 2017. Upon physical examination, the snake (Code no. LS-KJ 11) was male, measured 117.6 cm long (snout to vent-length) with a weight of 966.5 g. It was equipped with the morphological characteristics of L. semifasciata [6]. During the rearing in captivity, it was fed Korean sand lance, Hypoptychus dybowskii Steindachnerm 1880 (stored frozen) every 2 weeks, and the temperature of the water tank was maintained at approximately 23°C (±2°C). The snake died 3 months after its capture.

During the necropsy, noteworthy lesions were not observed except for the lumen of the lungs. Lung lobes showed bloody foamy exudates, around which 21 reddish elongated flukes were found (Fig. 1). And 5 small tongue-shaped flukes were found from the intestine. They were washed in 0.9% saline and then transferred into 70% ethyl alcohol or 10% neutral-buffered formalin for further molecular and morphological studies.

Fig. 1

Gross lesion of a lung in a Chinese sea snake from the Republic of Korea. The lung lesion showing foamy bloody exudate with elongated parasites, Pulmovermis semifasciata (arrows). Scale bar=2 cm.

The specimens for morphological observation were examined following staining with Semichon’s acetocarmine solution, dehydrated using graded alcohol series, cleared in xylene, and mounted permanently in Permount (Fisher Scientific). Observations were conducted under a light microscope (Olympus BX-53, Tokyo, Japan). The drawings were made using the microscope, which was equipped with a DIC system and drawing tube. Measurements were taken and calculated from drawings. Some measurements were cross-checked by comparison with measurements using a micrometer equipped on the eyepiece of light microscope.


Pulmovermis cyanovitellosus Coil & Kuntz, 1960

Description of worms was based on 4 fully mature worms as whole-mounted specimens (Fig. 2). Measurements are shown in Table 1. Body large, elongate, cylindrical, with maximum width at anterior to ventral sucker or posterior to ovary. Thinnest between ventral sucker and anterior testis when it stretched. Forebody very short. Ecsoma reduced. Body surface smooth or covered with small papillae.

Fig. 2

Pulmovermis cyanovitellosus, drawings of adult worm found from a Chinese sea snake Laticauda semifasciata. (A) Entire worm. (B) Terminal genitalia. Scale bar: (A) 4 mm; (B) 500 μm.

Morphometric comparison of Pulmovermis cyanovitellosus found from Chinese sea snake with those of previous studies (unit: μm)

Oral sucker well-developed, subglobular, muscular, subterminal. Pharynx melon-shaped, well-developed, muscular. Prepharynx and oesophagus absent. Ventral sucker muscular, subglobular, with deep cavity, slightly larger than oral sucker, project to somewhat ventrally, located close to anterior extremity. Intestinal bifurcation at level of pharynx; caeca blind, connect somewhat anterior, and then extend along each side of body to posterior extremity.

Testes two, tandem, elongate-oval, smooth, separated, located in third quarter of body. Seminal vesicle tubular, very long, surrounded by thick muscular walls. Genital pore posterior to pharynx. Cirrus short tubular. Ovary subspherical, located in posterior quarter of body. Vitellarium posterior to ovary, divided into 7 unnoticed lobes. Uterus tubular, filled with numerous eggs, extensively located in posterior part of body, connected along bodyline to genital pore linearly. Eggs very small.

Harmotrema laticaudae Yamaguti, 1933

Description of worms was based on 4 fully mature worms as whole-mounted specimens (Fig. 3). Measurements are shown in Table 2. Body tongue-shaped, elongate, with blunt to round anterior and posterior extremity, maximum width at level of cirrus sac. Forebody short. Body surface smooth.

Fig. 3

Harmotrema laticaudae, drawings of adult worm found from a Chinese sea snake Laticauda semifasciata. (A) Entire worm. (B) Terminal genitalia. Scale bar=1 mm (A), 250 μm (B).

Morphometric comparison of Harmotrema laticaudae with original description (unit: μm)

Oral sucker spherical, terminal. Prepharynx absent or short, Pharynx muscular, similar with oral sucker in size. Oesophagus short. Intestinal bifurcation just somewhat behind to pharynx, caeca blind, extend along the line between lateral body line and median line to posterior extremity. Ventral sucker spherical, larger than oral sucker. located 20% region from anterior extremity. Excretory system well-developed, paired inter- and extracaecal tubules united at anteriorly to intestinal bifurcation and posterior extremity.

Testes two, tandem. intercecal, distribute to third quarter of body, separated. Anterior testis located at midlevel of body, spherical slightly broader. Posterior testis located at 1,582 μm away from posterior extremity, spherical. Cirrus sac large, slightly obliquely positioned, anterior to anterior testis. Genital pore sinistral, intercecal. Seminal vesicle bipartite, proximal part oval, distal part elongated, surrounded by prostatic cells. Ejaculatory duct and cirrus armed with minute spines.

Ovary located between 2 testes, oval, much smaller than testes. Vitelline follicles minutes, distribute from somewhat anteriorly to posterior extremity to posterior to ventral sucker. Uterus occurred at level of ovary, extend anteriorly to genital pore through ventral side of body, coiled, filled with eggs. Metraterm well-developed, composed by strong muscles, occupying posterosinistral region of cirrus sac. Eggs oval.


Sea snakes are typically found in the warm waters of tropical and subtropical seas belonging to the Indian and Pacific oceans. However, sea snakes have recently been found outside of their typical habitats. Recent reports indicate these out-of habitat findings have occurred on the mainland of Japan, the Russian Far East, and California, USA [8,9,15]. This phenomenon is believed to be the effect of global warming, and the increasing appearances of sea snakes in Korean waters are also thought to be due to the same reason [10]. Laticauda semifasciata is one of 2 sea snake species that was recently discovered in Korean waters [6,14]. The typical distribution is marine areas around China, Taiwan, the southern Islands of Japan, Philippines, and Indonesia [16]. Furthermore, the occurrences and observations of L. semifasciata in Korea have been increasing. Park et al. [10] analyzed the haplotype network using partial sequences of mitochondrial cytochrome b (Cytb) genes extracted from 12 L. semifasciata caught in Korean waters, and confirmed the northward dispersal of the species. However, the parasites of the sea snakes in Korean waters have not been investigated until the present study.

In this study, we identified 2 species of digenean flukes that have distinctly different morphological characteristics, from a Chinese sea snake that died in a breeding environment. We provided the morphological descriptions and measurements of these Korean isolates and compared them to previously reported cases. Morphological identification indicates the present specimens are digenean fluke species that have been reported before from same host species, L semifasciata. Based on morphological characteristics and morphometric analysis (Tables 1, 2), the 2 species of digenean flukes were identified as P. cyanovitellosus, from the lungs, and H. laticaudae, from the intestines. Both of the morphology and measurements fit well with the previously recorded morphology and measurements of each species [11,12,30]. Information regarding the digenean flukes of sea snakes is scarcely reported. However, these 2 species have been recorded relatively frequently in areas such as Taiwan and Ryukyu Islands [1113]. Considering these factors, the existence of the 2 species of digenean flukes in L. semifasciata was suspected to be related to the origin of its host.

The body shape of P. cyanovitellosus in the present study is noteworthy, and it highlights that further studies regarding the validity of the genus Hydrophitrema Sandars, 1960, and its only known species, H. giganticum Sandars, 1960 (also known as H. gigantica, emended after follows WoRMS [17]). The genus Hydrophitrema was determined based on 6 specimens obtained from the lungs of an elegant sea snake Hydrophis elegans (Gray, 1842) caught from Moreton Bay, Queensland, Australia just 2 days after the discovery of P. cyanovitellosus was published [18,19]. Sandars [19] observed specimens of both Pulmovermis and Hydrophitrema, and noted they are closely related, and it seemed likely the 2 genera were synonymous, but they can be accepted as 2 distinct genera based on several factors. Sandars [19] erected a subfamily (Pulmoverminae Sandars, 1961) and noted the first comparisons between Pulmovermis and Hydrophitrema. Vercammen-Grandjean and Heyneman (1964) corrected some erroneously recorded factors described by Sandars (1960, 1961), including the body size of H. giganticum (17–26 mm rather than 170–260 mm) and the seminal vesicle to body length ratio (1:4.7 rather than 1:47), and suggested comparisons based on the corrected measurements of Sandars (1960), 2 types of specimens of P. cyanovitellosus, his own collection of H. giganticum, and the drawings of the original paper [1820]. According to their proposal, both genera can be distinguished mainly by the following factors: 1) general body shape (elongate tubular shape vs. elongate oblong shape), 2) the position and shape of the acetabulum, 3) the degree of seminal vesicle coiling, 4) the arrangement of the testes (tandem vs. oblique), and 5) the presence of tegumental folds or thickening enclosing both suckers in Hydrophitrema [20]. The validity of the genus Hydrophitrema was well-accepted by several authors until the 1970s [2023]. However, after Gibson et al. [24] listed the genus Hydrophitrema as a junior synonym of genus Pulmovermis, the validity of the genus Hydrophitrema has been neglected and not been discussed further [24,25]. Furthermore, H. giganticum was also regarded to be synonymous with P. cyanovitellosus [17,26,27].

In our opinion, the genus Hydrophitrema and/or the type species H. giganticum should be re-assessed and validated. In the present study, our specimens had thin and elongated tubular bodies, with thin body parts between the ventral sucker and anterior testis. The ventral sucker was located at 7–12% (~10%) of the body length and strongly developed with peduncles. The seminal vesicle was surrounded by a thick muscular layer and positioned in the anterior body, somewhat posterior to the ventral sucker. The testes were tandem in arrangement. Tegumental folds or thickening around both suckers were not observed. These features clearly satisfied the morphological characteristics of P. cyanovitellosus and comparisons provided by Vercammen-Grandjean et al. [20]. In addition, P. cyanovitellosus, observed in the picture of gross lesion at the necropsy in the present study (Fig. 1), seems well-distinguished from the pictures of the lung fluke thought to be H. giganticum (see Gillett et al., 2017 Fig. 8A, B) [27]. The elongate-tubular body is likely being the typical body shape of the species or even the genus, because both flexed and contracted specimens showing similar body form. Against this background, we temporarily decided to deem both species as valid in the present study.

The descriptions and drawings provided by previous literature indicate only the pulmovermins found from sea kraits belonging to the subfamily Laticaudinae, showing the morphological characteristics of P. cyanovitellosus as described above [1113,19,20]. On the other hand, trematode parasites that have the typical morphological characteristics of H. giganticum were only detected in hydrophinii sea snakes [1820,2223,27]. In our case, the parasites shared a laticaudinii definitive host, L. semifasciata, with previous reports of P. cyanovitellosus. The host differences between P. cyanovitellosus and H. giganticum were thought to be one of the important factors differentiating their genuine taxonomic status.

Pulmovermis cyanovitellosus was first reported by Coil and Kuntz [11] from the lungs of L. semifasciata located off the coast of Yeh Yu, the west coast of Lan Yu Island in Taiwan. Six years later, Telford [12] investigated the parasites of L. semifasciata located off Amami Island, Japan, and found a species of lung fluke. Because he overlooked the publication of Coil and Kuntz [11] during preparation of his study, he named his specimens as Laticaudatrema amamiensis. Telford [28] later realized, and regarded his species synonymous to P. cyanovitellosus. Further studies revealed the additional distribution sites of this species, i.e., Ishigaki Island and Kudaka Island, Japan [13,29]. The present case is the fifth and northernmost report of the species.

In 1933, Yamaguti examined the common sea krait Laticauda laticauda, from Ishigaki Island, Japan, and found 3 specimens of digenean flukes in the small intestines. He placed his specimens under the genus Harmotrema Nicoll, 1914, and reported the species as a new species, namely Harmotrema laticaudae [30]. An additional report was conducted by Telford [12] off Amami Island, Japan. He found 10 infected snakes out of 51 L. semifasciata. He noted his specimens agreed well with H. laticaudae, but he did not leave any detailed morphological descriptions except for a picture. Additional host and location data of this species was obtained by Brooks et al. [31], off Australia. The morphological characteristics and morphometric data of the present specimens (n=4) are well-fitted to the data provided by Yamaguti [30], except for certain characteristics, for example all the specimens had inverted cirri in the present study. In Yamaguti’s description, the vitelline follicles were distributed to the posterior margin of the ventral suckers, and he mentioned the vitelline follicles extending from the ventral sucker zone [30]. In our specimens, depends on the specimen, the vitelline follicles were distributed to the posterior margin of the ventral sucker or extended with some distance from posterior margin of ventral sucker. Thus, the anterior distribution of the vitelline follicles does not appear to have high taxonomic value.

Conclusively, we collected 2 digenean trematode species from a Chinese sea snake, L. semifasciata, which was caught offshore of Jeju Island, Korea. Based on morphological identification, we identified the worms as P. cyanovitellosus and H. laticaudae, and both species have not been previously reported in Korean parasitic fauna. We newly detailed and recorded their morphological descriptions. The taxonomic status of the genera Pulmovermis and Hydrophitrema were also discussed based on a comparison between the information obtained in the present study and previous literature. The differences between the 2 genera were re-verified, and we provided new insight in terms of host ranges. However, the taxonomic value remains questionable and further research is required; our observations were restricted to a group of parasites found within a host without comparisons to genuine specimens collected from hydrophinii sea snakes. The results of this study should be corroborated with additional observations of specimens collected from both hydrophinii and laticaudinii sea snakes. Additional studies, including DNA analyses, will be helpful in confirming taxonomic status and provide further insight into the likely ecology of parasites in Korea.


This work was supported by a grant from the National Institute of Biological Resources (NIBR), which is funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR no. 2019-02-001) and the Parasite Resource Bank of Korea of the National Research Resource Center (2012-0000037) of the Republic of Korea. This study was supported by grants from the National Marine Biodiversity Institute of Korea (2020M00300).



The authors declare that they have no conflicts of interest regarding the publication of this article.


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Fig. 1

Gross lesion of a lung in a Chinese sea snake from the Republic of Korea. The lung lesion showing foamy bloody exudate with elongated parasites, Pulmovermis semifasciata (arrows). Scale bar=2 cm.

Fig. 2

Pulmovermis cyanovitellosus, drawings of adult worm found from a Chinese sea snake Laticauda semifasciata. (A) Entire worm. (B) Terminal genitalia. Scale bar: (A) 4 mm; (B) 500 μm.

Fig. 3

Harmotrema laticaudae, drawings of adult worm found from a Chinese sea snake Laticauda semifasciata. (A) Entire worm. (B) Terminal genitalia. Scale bar=1 mm (A), 250 μm (B).

Table 1

Morphometric comparison of Pulmovermis cyanovitellosus found from Chinese sea snake with those of previous studies (unit: μm)

Organ Coil & Kuntz, 1960 Telford, 1967 Present study (average)
Body length 17,000 10,900–21,300 12,022–25,056 (19,404)
 width, maximum 1,200 800–1,620 831–1,079 (933)
  minimum 180–427 (262)

Oral sucker length 290–530 530–710 517–629 (570)
 width 450–660 562–685 (612)

Pharynx length 200–300 290–400 258–315 (284)
 width 230–340 180–315 (275)

Seminal vesicle length 3,258–5,978 (4,736)

Ventral sucker length 440–730 700–1,230 652–921 (781)
 width 680–1,180 787–966 (876)

Anterior extremity to ventral sucker 1,483–2,157 (1,809)

Anterior testis length 580–970 540–1,400 562–1,011 (848)
 width 350–620 292–404 (337)

Posterior testis length 1290–1,440 472–1,191 (938)
 width 350–660 292–449 (362)

Distance between testes 225–2,202 (1,258)

Ovary length 530–900 430–750 270–427 (340)
 width 420–740 247–494 (357)

Vitellaria length 530–900 472–719 (646)
 width 292–584 (421)

Egg* length 17–23 14–18 (17)
 width 10–12 7–9 (9)


Table 2

Morphometric comparison of Harmotrema laticaudae with original description (unit: μm)

Organs Laticauda laticauda
Yamaguti, 1933
Laticauda semifasciata
Present study (average)
Body length 5,700–6,900 6,022–6,818 (6,433)
 width 1,000–2,000 745–1,136 (975)

Oral sucker length 75–100 82–123 (91)
 width 110–120 109–141 (117)

Pharynx length 90–110 100–109 (102)
 width 100–127 (111)

Prepharynx length 0–5

Esophagus length 40–80 118–150 (134)

Anterior end to intestinal bifurcation 327–331 (330)

Ventral sucker length 110–130 127–191 (161)
 width 110–175 127–191 (159)

Anterior extremity to ventral sucker 1,209–1,309 (1,266)

Cirrus sac length 600–664 (625)
 width 318–382 (350)

Seminal vesicle length 400–482 (448)
 width 282–300 (290)

Metraterm length 518–691 (586)
 width 209–300 (255)

Anterior testis length 500–550 382–509 (455)
 width 440–600 382–436 (411)

Posterior testis length 600–700 473–573 (511)
 width 450–560 350–382 (372)

Distance between testes 0–745 (477)

Ovary length 160–250 136–209 (182)
 width 230–310 164–281 (191)

Egg* length 123–129 111–125 (123)*
 width 75–78 59–77 (68)*