With the aid of a local Korean medical service in Cambodia, we collected 623 stool samples from children at Watkor Kindergarten and from schoolchildren in Bat Dambang, Cambodia in March 2004; Bat Dambang is located about 250 km southeast of Phnom Penh, the capital city of Cambodia.

Formalin-ether sedimentation revealed intestinal helminth eggs of Hookworm, Rhabditis sp., Hymenolepis nana, and Echinostoma sp., and protozoan cysts of Entamoeba histolytica, Entamoeba coli, Giardia lamblia, Iodamoeba butschlii, and Entamoeba polecki. Of the 623 stool specimens, 160 (25.7%) were positive. The positive rates for boys and girls were 26.2% (90/344) and 25.1% (70/279), respectively. Data revealed a highest positive rate (4.8%) for Echinostoma sp., followed by 3.4% for Hookworm (Table 1). Multiple parasite infections were found in 9 cases. Infection rates ranged from 10% to 38.2% by school grade; the highest rate was found among third grade of primary schoolchildren. The results of a comparison of trematode infection and eating habits were interesting. In general, children did not consume raw fresh water fish (Story et al., 1989); however, they were believed to have been infected by inadequately cooked fish. Echinostoma species are important trematode parasites and require treatment; anti-helminthics were sent to the Watkor primary school after the stool examination.

The school is located along the Steung Sangkae River, which is connected to Tonle Sab Lake. Although many children swam in this river during the stool examination period, we failed to find Schistosoma eggs. Accordingly, in view of their swimming habits, this area is not considered to be a schistosomiasis endemic area.

The present study showed different patterns of intestinal parasite infection compared to the previous survey at Kampongcham (Lee et al., 2002); this survey showed no cases of Ascaris lumbricoides, Trichuris trichiura or Opisthorchis sp., while egg positive rates of A. lumbricoides, T. trichiura, and Opisthorchis sp. in Kampongcham were 26.3%, 1%, and 10%, respectively. However, children in Bat Dambang were found infected with E. polecki (1.1%) and H. nana (1.3%), whereas no cases of E. polecki or H. nana were recorded in the Kampongcham survey in 2002 (Lee et al., 2002). This study identified regional differences in intestinal parasitic infections between in Bat Dambang and in Kampongcham.

Periodic surveys on intestinal parasitic infections, the treatment of infected cases, and education with respect to eating habits and improved sanitation conditions are highly recommended for parasite control in Cambodia.