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Chul-Yong Song	2 Articles
Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against experimental Neospora caninum infection Jung-Hwa Cho, Woo-Suk Chung, Kyoung-Ju Song, Byoung-Kuk Na, Seung-Won Kang, Chul-Yong Song, Tong-Soo Kim Korean J Parasitol 2005;43(1):19-25. Published online March 20, 2005 DOI: https://doi.org/10.3347/kjp.2005.43.1.19 Abstract PDF Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against N. caninum infection was evaluated in vitro and in vivo. Two major immunodominant surface antigens (NcSAG1 and NcSRS2) and two dense granule proteins (NcDG1 and NcDG2) of N. caninum tachyzoites were expressed in E. coli, respectively. An in vitro neutralization assay using polyclonal antisera raised against each recombinant antigen showed inhibitory effects on the invasion of N. caninum tachyzoites into host cells. Separate groups of gerbils were immunized with the purified recombinant proteins singly or in combinations and animals were then challenged with N.caninum. Following these experimental challenges, the protective efficacy of each vaccination was determined by assessing animal survival rate. All experimental groups showed protective effects of different degrees against experimental infection. The highest protection efficacy was observed for combined vaccination with NcSRS2 and NcDG1. Our results indicate that combined vaccination with the N. caninum recombinant antigens, NcSRS2 and NcDG1, induces the highest protective effect against N. caninum infection in vitro and in vivo. Citations Citations to this article as recorded by Molecular characterization of Neospora caninum major antigens NcSAG1 and NcSRS2 Soledad Echeverría, Federico Carrión, Martín Soñora, Andrés Cabrera, Carlos Robello Royal Society Open Science.2025;[Epub] CrossRef A Listeria monocytogenes-Based Vaccine Formulation Reduces Vertical Transmission and Leads to Enhanced Pup Survival in a Pregnant Neosporosis Mouse Model Dennis Imhof, William Robert Pownall, Camille Monney, Anna Oevermann, Andrew Hemphill Vaccines.2021; 9(12): 1400. CrossRef Interferon-γ-dependent protection against Neospora caninum infection conferred by mucosal immunization in IL-12/IL-23 p40-deficient mice Pedro Ferreirinha, Ricardo Fróis-Martins, Luzia Teixeira, António Rocha, Manuel Vilanova, Alexandra Correia Vaccine.2018; 36(32): 4890. CrossRef On the application of reverse vaccinology to parasitic diseases: a perspective on feature selection and ranking of vaccine candidates Stephen J. Goodswen, Paul J. Kennedy, John T. Ellis International Journal for Parasitology.2017; 47(12): 779. CrossRef Mucosal immunization confers long-term protection against intragastrically established Neospora caninum infection Pedro Ferreirinha, Alexandra Correia, Maria Teixeira-Coelho, Hugo Osório, Luzia Teixeira, António Rocha, Manuel Vilanova Vaccine.2016; 34(50): 6250. CrossRef A 78 kDa host cell invasion protein of Neospora caninum as a potential vaccine candidate Qiang Lv, Shenyang Xing, Pengtao Gong, Le Chang, Zhengzheng Bian, Lidong Wang, Xichen Zhang, Jianhua Li Experimental Parasitology.2015; 148: 56. CrossRef Discovering a vaccine against neosporosis using computers: is it feasible? Stephen J. Goodswen, Paul J. Kennedy, John T. Ellis Trends in Parasitology.2014; 30(8): 401. CrossRef Cloning and expression of Neospora caninum dense-granule 7 in E. coli Marziye Kefayat, Hossein Hamidinejat, Masoud Reza Seifiabadshapoori, Mohammad Mehdi Namavari, Parviz Shayan, Saad Gooraninejad Journal of Parasitic Diseases.2014; 38(2): 196. CrossRef Vaccines against neosporosis: What can we learn from the past studies? Thierry Monney, Andrew Hemphill Experimental Parasitology.2014; 140: 52. CrossRef A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge Yanina P. Hecker, Verónica Cóceres, Silvina E. Wilkowsky, José M. Jaramillo Ortiz, Eleonora L. Morrell, Andrea E. Verna, Agustina Ganuza, Dora B. Cano, Lilian Lischinsky, Sergio O. Ángel, Patricia Zamorano, Anselmo C. Odeón, María R. Leunda, Carlos M. Cam Veterinary Immunology and Immunopathology.2014; 162(3-4): 142. CrossRef A guide to in silico vaccine discovery for eukaryotic pathogens S. J. Goodswen, P. J. Kennedy, J. T. Ellis Briefings in Bioinformatics.2013; 14(6): 753. CrossRef ELISAs based on rNcGRA7 and rNcSAG1 antigens as an indicator of Neospora caninum activation Jun Hiasa, Junko Kohara, Maki Nishimura, Xuenan Xuan, Hiromi Tokimitsu, Yoshifumi Nishikawa Veterinary Parasitology.2012; 187(3-4): 379. CrossRef Vaccines against a Major Cause of Abortion in Cattle, Neospora caninum Infection Thierry Monney, Karim Debache, Andrew Hemphill Animals.2011; 1(3): 306. CrossRef Comparative efficacy of immunization with inactivated whole tachyzoites versus a tachyzoite-bradyzoite mixture against neosporosis in mice S. ROJO-MONTEJO, E. COLLANTES-FERNÁNDEZ, S. RODRÍGUEZ-MARCOS, F. PÉREZ-ZABALLOS, I. LÓPEZ-PÉREZ, A. PRENAFETA, L. M. ORTEGA-MORA Parasitology.2011; 138(11): 1372. CrossRef Transgenic Neospora caninum strains constitutively expressing the bradyzoite NcSAG4 protein proved to be safe and conferred significant levels of protection against vertical transmission when used as live vaccines in mice V. Marugán-Hernández, L.M. Ortega-Mora, A. Aguado-Martínez, E. Jiménez-Ruíz, G. Álvarez-García Vaccine.2011; 29(44): 7867. CrossRef Selection of Neospora caninum antigens stimulating bovine CD4+ve T cell responses through immuno-potency screening and proteomic approaches Mara S Rocchi, Paul M Bartley, Neil F Inglis, Esther Collantes-Fernandez, Gary Entrican, Frank Katzer, Elisabeth A Innes Veterinary Research.2011; 42(1): 91. CrossRef RecNcMIC3-1-R is a microneme- and rhoptry-based chimeric antigen that protects against acute neosporosis and limits cerebral parasite load in the mouse model for Neospora caninum infection Thierry Monney, David Rütti, Michelle Schorer, Karim Debache, Denis Grandgirard, Stephen L. Leib, Andrew Hemphill Vaccine.2011; 29(40): 6967. CrossRef Immunization of female BALB/c mice with Neospora cyclophilin and/or NcSRS2 elicits specific antibody response and prevents against challenge infection by Neospora caninum Wenbin Tuo, Yan Zhao, Daming Zhu, Mark C. Jenkins Vaccine.2011; 29(13): 2392. CrossRef Intraperitoneal and intra-nasal vaccination of mice with three distinct recombinantNeospora caninumantigens results in differential effects with regard to protection against experimental challenge withNeospora caninumtachyzoites K. DEBACHE, C. GUIONAUD, F. ALAEDDINE, A. HEMPHILL Parasitology.2010; 137(2): 229. CrossRef Characterisation of NcGRA7 and NcSAG4 proteins: Immunolocalisation and their role in the host cell invasion by Neospora caninum tachyzoites Adriana Aguado-Mart?nez, Gema ?lvarez-Garc?a, Gereon Schares, Ver?nica Risco-Castillo, Aurora Fern?ndez-Garc?a, Virginia Marug?n-Hern?ndez, Luis Ortega-Mora Acta Parasitologica.2010;[Epub] CrossRef CpG-ODN combined with Neospora caninum lysate, but not with excreted-secreted antigen, enhances protection against infection in mice Dâmaso P. Ribeiro, Marina M.P. Freitas, Mariana R.D. Cardoso, Ana C.A.M. Pajuaba, Neide M. Silva, Tiago W.P. Mineo, João S. Silva, José R. Mineo, Deise A.O. Silva Vaccine.2009; 27(19): 2570. CrossRef Characterization of tissue distribution and histopathological lesions in Neospora caninum experimentally infected gerbils Seung-Won Kang, Sung-Soo Park, Se-Eun Choe, Young-Hwa Jean, Suk-Chan Jung, Keun Kim, Dong Van Quyen Parasitology Research.2009; 104(6): 1261. CrossRef Failure of a vaccine using immunogenic recombinant proteins rNcSAG4 and rNcGRA7 against neosporosis in mice Adriana Aguado-Martínez, Gema Álvarez-García, Aurora Fernández-García, Verónica Risco-Castillo, Virginia Marugán-Hernández, Luis M. Ortega-Mora Vaccine.2009; 27(52): 7331. CrossRef Vaccination with recombinant NcROP2 combined with recombinant NcMIC1 and NcMIC3 reduces cerebral infection and vertical transmission in mice experimentally infected with Neospora caninum tachyzoites Karim Debache, Ferial Alaeddine, Christophe Guionaud, Thierry Monney, Joachim Müller, Maria Strohbusch, Stephen L. Leib, Denis Grandgirard, Andrew Hemphill International Journal for Parasitology.2009; 39(12): 1373. CrossRef Neospora caninum – How close are we to development of an efficacious vaccine that prevents abortion in cattle? Michael P. Reichel, John T. Ellis International Journal for Parasitology.2009; 39(11): 1173. CrossRef Immunization with Oligomannose-Coated Liposome-Entrapped Dense Granule Protein 7 Protects Dams and Offspring fromNeospora caninumInfection in Mice Yoshifumi Nishikawa, Houshuang Zhang, Yuzuru Ikehara, Naoya Kojima, Xuenan Xuan, Naoaki Yokoyama Clinical and Vaccine Immunology.2009; 16(6): 792. CrossRef Vaccination of mice with recombinant NcROP2 antigen reduces mortality and cerebral infection in mice infected with Neospora caninum tachyzoites Karim Debache, Christophe Guionaud, Ferial Alaeddine, Meike Mevissen, Andrew Hemphill International Journal for Parasitology.2008; 38(12): 1455. CrossRef Prevention of lethal experimental infection of C57BL/6 mice by vaccination with Brucella abortus strain RB51 expressing Neospora caninum antigens Sheela Ramamoorthy, Neelima Sanakkayala, Ramesh Vemulapalli, Robert B. Duncan, David S. Lindsay, Gerhart S. Schurig, Stephen M. Boyle, Ramanathan Kasimanickam, Nammalwar Sriranganathan International Journal for Parasitology.2007; 37(13): 1521. CrossRef Reduced cerebral infection of Neospora caninum in BALB/c mice vaccinated with recombinant Brucella abortus RB51 strains expressing N. caninum SRS2 and GRA7 proteins Ramesh Vemulapalli, Neelima Sanakkayala, Jatinder Gulani, Gerhardt G. Schurig, Stephen M. Boyle, David S. Lindsay, Nammalwar Sriranganathan Veterinary Parasitology.2007; 148(3-4): 219. CrossRef VACCINATION WITH MICRONEME PROTEIN NCMIC4 INCREASES MORTALITY IN MICE INOCULATED WITH NEOSPORA CANINUM Sangeetha Srinivasan, Joachim Mueller, Angela Suana, Andrew Hemphill Journal of Parasitology.2007; 93(5): 1046. CrossRef Protecting babies: vaccine strategies to prevent foetopathy inNeospora caninum‐infected cattle D. J. L. WILLIAMS, A. J. TREES Parasite Immunology.2006; 28(3): 61. CrossRef Vaccination as a control strategy against the coccidial parasitesEimeria,ToxoplasmaandNeospora E. A. INNES, A. N. VERMEULEN Parasitology.2006; 133(S2): S145. CrossRef Immunization with native surface protein NcSRS2 induces a Th2 immune response and reduces congenital Neospora caninum transmission in mice G.J. Haldorson, B.A. Mathison, K. Wenberg, P.A. Conrad, J.P. Dubey, A.J. Trees, I. Yamane, T.V. Baszler International Journal for Parasitology.2005; 35(13): 1407. CrossRef 8,942 View 94 Download Crossref Degradation of immunoglobulins, protease inhibitors, and interleukin-1 by a secretory proteinase of Acanthamoeba castellanii Byoung-Kuk Na, Jong-Hwa Cho, Chul-Yong Song, Tong-Soo Kim Korean J Parasitol 2002;40(2):93-99. Published online June 30, 2002 DOI: https://doi.org/10.3347/kjp.2002.40.2.93 Abstract PDF The effect of a secretory proteinase from the pathogenic amoebae Acanthamoeba castellanii on host's defense-oriented or regulatory proteins such as immunoglobulins, interleukin-1, and protease inhibitors was investigated. The enzyme was found to degrade secretory immunoglobulin A (sIgA), IgG, and IgM. It also degraded interleukin-1α (IL-1α) and IL-1β. Its activity was not inhibited by endogenous protease inhibitors, such as α2-macroglobulin, α1-trypsin inhibitor, and α2-antiplasmin. Furthermore, the enzyme rapidly degraded those endogenous protease inhibitors as well. The degradation of host's defense-oriented or regulatory proteins by the Acanthamoeba proteinase suggested that the enzyme might be an important virulence factor in the pathogenesis of Acanthamoeba infection. 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