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Chungryul Kim	1 Article
Enzymatic Activities of Allergen Extracts from Three Species of Dust Mites and Cockroaches Commonly Found in Korean Home Kyoung Yong Jeong, Chungryul Kim, Tai-Soon Yong Korean J Parasitol 2010;48(2):151-155. Published online June 17, 2010 DOI: https://doi.org/10.3347/kjp.2010.48.2.151 Abstract PDF Allergen extracts from dust mites and cockroaches commonly found in Korean homes were used to evaluate their enzymatic activity as they are believed to influence allergenicity. Allergen extracts were prepared from 3 dust mite species (Dermatophagoides farinae, D. pteronyssinus, and Tyrophagus putrescentiae) and 3 cockroach species (Blattella germanica, Periplaneta americana, and P. fuliginosa) maintained in the Korea National Arthropods of Medical Importance Resource Bank. Proteins were extracted in PBS after homogenization using liquid nitrogen. The activities of various enzymes were investigated using the API Zym system. No significant difference in phosphatase, lipase, or glycosidase activity was observed among the 6 allergen extracts, but much difference was observed in protease activity. Protease activity was assessed in more detail by gelatin zymography and the EnzChek assay. Extract from T. putrescentiae showed the highest protease activity, followed by those of the cockroach extracts. Extracts from D. farinae and D. pteronyssinus showed only weak protease activity. Gelatinolytic activity was detected mainly in a 30-kDa protein in D. farinae, a 28-kDa protein in D. pteronyssinus, a > 26-kDa protein in T. putrescentiae, a > 20-kDa protein in B. germanica, and a > 23-kDa protein in P. americana and P. fuliginosa. The information on various enzymatic activities obtained in this study may be useful for future studies. In particular, the strong protease activity found in cockroach extracts could contribute to sensitization to cockroach allergens, which is known to be associated with the development of asthma. Citations Citations to this article as recorded by Application of Photocatalyzed TiO2 for Inactivation of Inhalant Allergens Yoon Ji Shin, Haeun Kim, Minji Hwang, Seung Jae Baeck, Jung-Won Park, Kyoung Yong Jeong International Archives of Allergy and Immunology.2025; 187(1): 1. CrossRef B-cell antibody class switchings are pressuromodulated events: Part II, gene recombination Hemant Sarin Translational Medicine Communications.2018;[Epub] CrossRef Allergenic Characterization of a Novel Allergen, Homologous to Chymotrypsin, from German Cockroach Kyoung Yong Jeong, Mina Son, Jae-Hyun Lee, Chein-Soo Hong, Jung-Won Park Allergy, Asthma & Immunology Research.2015; 7(3): 283. CrossRef Current Status of Standardization of Inhalant Allergen Extracts in Korea Kyoung Yong Jeong, Jae-Hyun Lee, Eun-Jin Kim, Joo-Shil Lee, Sang-Heon Cho, Soo-Jong Hong, Jung-Won Park Allergy, Asthma & Immunology Research.2014; 6(3): 196. CrossRef Preparation and Characterization of an Extract of German Cockroach From a Korean Source Kyoung Yong Jeong, Soo-Young Choi, Jae-Hyun Lee, Joo-Shil Lee, Tai-Soon Yong, Chein-Soo Hong, Jung-Won Park Allergy, Asthma & Immunology Research.2013; 5(2): 102. CrossRef The Effects of Storage Conditions on the Stability of House Dust Mite Extracts Kyoung Yong Jeong, Soo-Young Choi, In-Soo Han, Jae-Hyun Lee, Joo-Shil Lee, Chein-Soo Hong, Jung-Won Park Allergy, Asthma & Immunology Research.2013; 5(6): 397. CrossRef House Dust Mite Allergy in Korea: The Most Important Inhalant Allergen in Current and Future Kyoung Yong Jeong, Jung-Won Park, Chein-Soo Hong Allergy, Asthma & Immunology Research.2012; 4(6): 313. CrossRef Role of Cockroach Proteases in Allergic Disease Kristen Page Current Allergy and Asthma Reports.2012; 12(5): 448. CrossRef Optimization of Allergen Standardization Kyoung Yong Jeong, Chein-Soo Hong, Joo-Shil Lee, Jung-Won Park Yonsei Medical Journal.2011; 52(3): 393. CrossRef 9,544 View 83 Download Crossref