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Dae-Yong Kim	4 Articles
Seroprevalence of antibodies to Neospora caninum in dogs and raccoon dogs in Korea Jae-Hoon Kim, Min-Soo Kang, Byung-Chun Lee, Woo-Suk Hwang, Chang-Woo Lee, Byung-Jae So, J. P. Dubey, Dae-Yong Kim Korean J Parasitol 2003;41(4):243-245. Published online December 20, 2003 DOI: https://doi.org/10.3347/kjp.2003.41.4.243 Abstract PDF Neospora caninum is an important cause of abortion in cattle, and dogs are its only known definitive host. Its seroprevalence among domestic urban and rural dogs and feral raccoon dogs (Nyctereutes procyonoides koreensis) in Korea was studied by indirect fluorescent antibody test (IFAT) and by the neospora agglutination test (NAT), respectively. Antibodies to N. caninum were found in 8.3% of urban dogs and in 21.6% of dogs at dairy farms. Antibody titers ranged from 1:50 to 1:400. Antibodies to N. caninum were found in six (23%) of 26 raccoon dogs. However, the potential role of raccoon dogs as a source of horizontal transmission of bovine neosporosis needs further investigation. The results of this study suggest that there is a close relationship between N. caninum infection among dairy farm dogs and cattle in Korea. This study reports for the first time upon the seroprevalence of N. caninum infection in raccoon dogs in Korea. Citations Citations to this article as recorded by First description of Hepatozoon canis in raccoon dog (Nyctereutes procyonoides) Itainara Taili, Jongseung Kim, Sungryong Kim, Dong-Hyuk Jeong, Ki-Jeong Na International Journal for Parasitology: Parasites and Wildlife.2025; 28: 101132. CrossRef Seroprevalence of Neospora caninum Infection in Dog Population Worldwide: A Systematic Review and Meta-analysis Davood Anvari, Reza Saberi, Mehdi Sharif, Shahabbedin Sarvi, Seyed Abdollah Hosseini, Mahmood Moosazadeh, Zahra Hosseininejad, Tooran Nayeri Chegeni, Ahmad Daryani Acta Parasitologica.2020; 65(2): 273. CrossRef First report of Neospora caninum seroprevalence in farmed raccoon dogs in China Lan-Bi Nie, Yang Zou, Jun-Ling Hou, Qin-Li Liang, Wei Cong, Xing-Quan Zhu Acta Tropica.2019; 190: 80. CrossRef RETROSPECTIVE ANALYSIS OF THE EPIDEMIOLOGIC LITERATURE, 1990–2015, ON WILDLIFE-ASSOCIATED DISEASES FROM THE REPUBLIC OF KOREA Jusun Hwang, Kyunglee Lee, Young-Jun Kim, Jonathan M. Sleeman, Hang Lee Journal of Wildlife Diseases.2017; 53(1): 5. CrossRef A review of neosporosis and pathologic findings of Neospora caninum infection in wildlife Shannon L. Donahoe, Scott A. Lindsay, Mark Krockenberger, David Phalen, Jan Šlapeta International Journal for Parasitology: Parasites and Wildlife.2015; 4(2): 216. CrossRef The biological potential of the raccoon dog (Nyctereutes procyonoides, Gray 1834) as an invasive species in Europe—new risks for disease spread? Astrid Sutor, Sabine Schwarz, Franz Josef Conraths Acta Theriologica.2014; 59(1): 49. CrossRef Control options forNeospora caninum– is there anything new or are we going backwards? MICHAEL P. REICHEL, MILTON M. McALLISTER, WILLIAM E. POMROY, CARLOS CAMPERO, LUIS M. ORTEGA-MORA, JOHN T. ELLIS Parasitology.2014; 141(11): 1455. CrossRef Evidence of Neospora caninum exposure among native Korean goats (Capra hircus coreanae) B.Y. Jung, S.H. Lee, D. Kwak Veterinární medicína.2014; 59(12): 637. CrossRef Neospora caninumand Wildlife Sonia Almería ISRN Parasitology.2013; 2013: 1. CrossRef Seroprevalence of Toxoplasma gondii and Neospora caninum in dogs from Korea Thuy Nguyen, Se-Eun Choe, Jae-Won Byun, Hong-Bum Koh, Hee-Soo Lee, Seung-Won Kang Acta Parasitologica.2012;[Epub] CrossRef Occurrence of antibodies to Neospora caninum and Toxoplasma gondii in dogs from Pernambuco, Northeast Brazil Luciana Aguiar Figueredo, Filipe Dantas-Torres, Eduardo Bento de Faria, Luis Fernando Pita Gondim, Lucilene Simões-Mattos, Sinval Pinto Brandão-Filho, Rinaldo Aparecido Mota Veterinary Parasitology.2008; 157(1-2): 9. CrossRef Seroprevalence of Neospora caninum in dogs in south-western Poland Katarzyna Płoneczka, Michał Mazurkiewicz Veterinary Parasitology.2008; 153(1-2): 168. CrossRef Epidemiology and Control of Neosporosis andNeospora caninum J. P. Dubey, G. Schares, L. M. Ortega-Mora Clinical Microbiology Reviews.2007; 20(2): 323. CrossRef Neospora caninum in wildlife Luís F.P. Gondim Trends in Parasitology.2006; 22(6): 247. CrossRef Sero-epidemiological survey of Neospora caninum infection in dogs in north-eastern Italy Gioia Capelli, Stefano Nardelli, Antonio Frangipane di Regalbono, Antonio Scala, Mario Pietrobelli Veterinary Parasitology.2004; 123(3-4): 143. CrossRef 8,285 View 83 Download Crossref ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera Hye-Jin Ahn, Sera Kim, Dae-Yong Kim, Ho-Woo Nam Korean J Parasitol 2003;41(3):175-177. Published online September 20, 2003 DOI: https://doi.org/10.3347/kjp.2003.41.3.175 Abstract PDF An ELISA was established to measure bovine IgG directed against the recombinant antigenic determinant of Nc-p43, a major surface antigen of Neospora caninum. In a previous study, two thirds of the C-terminal of the molecule was expressed as a 6 × His tagged protein (Ncp43P) for ELISA using 2/3 of the N-terminal of SAG1 from Toxoplasma gondii as a control (TgSAG1A). Among 852 cattle sera collected from stock farms scattered nation-wide, 103 sera (12.1%) were found to react with Ncp43P positively, but no positive reaction was observed with TgSAG1A. This study shows that Ncp43P could be available as an efficient antigen for the diagnosis of neosporosis in cattle. Furthermore, it together with TgSAG1A, could be useful for the differential diagnosis of N. caninum and T. gondii infections in other mammals. Citations Citations to this article as recorded by Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris for diagnosing neosporosis in sheep and dogs Amanda Fernandes Pinheiro, Sibele Borsuk, Maria Elisabeth Aires Berne, Luciano da Silva Pinto, Renato Andreotti, Talita Roos, Barbara Couto Roloff, Fábio Pereira Leivas Leite Revista Brasileira de Parasitologia Veterinária.2015; 24(2): 148. CrossRef Diagnostic Potential of Anti-rNcp-43 Polyclonal Antibodies for the Detection of Neospora caninum Gizele Lima de Sá, Diene de Borba Pacheco, Leonardo Garcia Monte, Francine Alves Sinnott, Marina Amaral Xavier, Caroline Rizzi, Sibele Borsuk, Maria Elisabeth Aires Berne, Renato Andreotti, Cláudia Pinho Hartleben Current Microbiology.2014; 68(4): 472. CrossRef Expression ofNeospora caninumNcSRS2 surface protein inPichia pastorisand its application for serodiagnosis ofNeosporainfection Amanda Fernandes Pinheiro, Sibele Borsuk, Maria Elisabeth Aires Berne, Luciano da Silva Pinto, Renato Andreotti, Talita Roos, Barbara Couto Rollof, Fábio Pereira Leivas Leite Pathogens and Global Health.2013; 107(3): 116. CrossRef Development of an indirect ELISA-NcSRS2 for detection of Neospora caninum antibodies in cattle Sibele Borsuk, Renato Andreotti, Fábio Pereira Leivas Leite, Luciano da Silva Pinto, Simone Simionatto, Claudia Pinho Hartleben, Marcelo Goetze, Leandra Marla Oshiro, Maria de Fátima Cepa Matos, Maria Elisabeth Aires Berne Veterinary Parasitology.2011; 177(1-2): 33. CrossRef Serodiagnosis of Neospora caninum infection in cattle using a recombinant tNcSRS2 protein-based ELISA Jing Liu, Jinshu Yu, Ming Wang, Qun Liu, Wei Zhang, Chong Deng, Jun Ding Veterinary Parasitology.2007; 143(3-4): 358. CrossRef Epidemiology and Control of Neosporosis andNeospora caninum J. P. Dubey, G. Schares, L. M. Ortega-Mora Clinical Microbiology Reviews.2007; 20(2): 323. CrossRef Diagnosis of bovine neosporosis: Recent advances and perspectives Luis Ortega-Mora, Aurora Fernández-García, Mercedes Gómez-Bautista Acta Parasitologica.2006; 51(1): 1. CrossRef Diagnosis of bovine neosporosis J.P. Dubey, G. Schares Veterinary Parasitology.2006; 140(1-2): 1. CrossRef Comparison of proteome and antigenic proteome between two Neospora caninum isolates Yong-Seung Shin, Gee-Wook Shin, Young-Rim Kim, Eun-Young Lee, Hyang-Hee Yang, K.J. Palaksha, Hee-Jeong Youn, Jae-Hoon Kim, Dae-Yong Kim, A.E. Marsh, J. Lakritz, Tae-Sung Jung Veterinary Parasitology.2005; 134(1-2): 41. CrossRef HPLC purification of recombinant NcGRA6 antigen improves enzyme-linked immunosorbent assay for serodiagnosis of bovine neosporosis M.C. Jenkins, R. Fetterer, G. Schares, C. Björkman, W. Wapenaar, M. McAllister, J.P. Dubey Veterinary Parasitology.2005; 131(3-4): 227. CrossRef Identification of antigenic proteins from Neospora caninum recognized by bovine immunoglobulins M, E, A and G using immunoproteomics Yong‐seung Shin, Eung‐goo Lee, Gee‐wook Shin, Young‐rim Kim, Eun‐young Lee, Jae‐hoon Kim, Hwan Jang, Laurel J. Gershwin, Dae‐yong Kim, Yong‐hwan Kim, Gon‐sup Kim, Myung‐deuk Suh, Tae‐sung Jung PROTEOMICS.2004; 4(11): 3600. CrossRef Development of competitive ELISA for neosporosis by employing immunoproteomics Yong-seung Shin, Eung-goo Lee, Gee-wook Shin, Young-rim Kim, Eun-young Lee, Jae-hoon Kim, Hwan Jang, Dae-yong Kim, Yong-hwan Kim, Gon-sup Kim, Myung-deuk Suh, Tae-sung Jung Clinical Proteomics.2004;[Epub] CrossRef 8,114 View 77 Download Crossref Determination of antigenic domain in GST fused major surface protein (Nc-p43) of Neospora caninum Eui-Sun Son, Hye-Jin Ahn, Jae-Hoon Kim, Dae-Yong Kim, Ho-Woo Nam Korean J Parasitol 2001;39(3):241-246. Published online September 30, 2001 DOI: https://doi.org/10.3347/kjp.2001.39.3.241 Abstract PDF The antigenic domain of the major surface protein (Nc-p43) of Neospora caninum was examined by polymerase chain reaction of its gene fragments and recombinant expression as GST fusion proteins. The fragments of Nc-p43 were as follow: a total open reading frame (OFR), T; OFR without signal sequence and C-terminal hydrophobic sequence, S; N-terminal 2/3 parts of S, A; C-terminal 2/3 parts, P; N-terminal 1/3 part, X; middle 1/3 part, Y; and C-terminal 1/3 part, Z, respectively. The DNA fragments were cloned into pGEX-4T vector. Recombinant plasmids transformed into Escherichia coli of BL21 pLysS (DE3) strain were induced to express GST or GST fused fragments of Nc-p43 such as 69 kDa protein for T, 66 kDa for S, 52 kDa for A, 53 kDa for P, and 40 kDa proteins for X, Y, and Z, respectively in SDS-PAGE. The Nc-p43 fragments of T, S, and P reacted with a bovine serum of neosporosis while those of A, X, Y, and Z together with GST did not in the western blot. These findings suggest that the antigenic domain of Nc-p43 of N. caninum may be localized in the C-terminal 2/3 parts. Together with A19 clone in SAG1 of Toxoplasma gondii (Nam et al., 1996), the P fragment of Nc-p43 could be used as efficient antigens to diagnose and differentiate those infections with both species. Citations Citations to this article as recorded by Molecular characterization of Neospora caninum major antigens NcSAG1 and NcSRS2 Soledad Echeverría, Federico Carrión, Martín Soñora, Andrés Cabrera, Carlos Robello Royal Society Open Science.2025;[Epub] CrossRef Expression ofNeospora caninumNcSRS2 surface protein inPichia pastorisand its application for serodiagnosis ofNeosporainfection Amanda Fernandes Pinheiro, Sibele Borsuk, Maria Elisabeth Aires Berne, Luciano da Silva Pinto, Renato Andreotti, Talita Roos, Barbara Couto Rollof, Fábio Pereira Leivas Leite Pathogens and Global Health.2013; 107(3): 116. CrossRef Induction of Interferon-Gamma (IFN-γ) and T Helper 1 (Th1) Immune Response by Bitter Gourd Extract Kazunori IKE, Yuko UCHIDA, Tomohiko NAKAMURA, Soichi IMAI Journal of Veterinary Medical Science.2005; 67(5): 521. CrossRef ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera Hye-Jin Ahn, Sera Kim, Dae-Yong Kim, Ho-Woo Nam The Korean Journal of Parasitology.2003; 41(3): 175. CrossRef 8,408 View 82 Download Crossref Detection of IgG antibody against Neospora caninum in cattle in Korea Ji-Seon Bae, Dae-Yong Kim, Woo-Suk Hwang, Jae-Hoon Kim, Nam-Seok Lee, Ho-Woo Nam Korean J Parasitol 2000;38(4):245-249. Published online December 31, 2000 DOI: https://doi.org/10.3347/kjp.2000.38.4.245 Abstract PDF A total of 492 cattle sera was screened by IgG-ELISA against Neospora caninum (Nc-1 strain and a Korean isolate, KBA-2) and Toxoplasma gondii. Out of 492, 113 sera (23.0%) reacted positively to either Nc-1 or KBA-2 strains of N. caninum. Among the 113 positive sera, 92 sera (81.4%) reacted with antigens of both strains, but 6 sera (5.3%) with Nc-1 and 15 sera (13.3%) with KBA-2 strain only. And with T. gondii antigen, 6 sera (1.2%) were positive but all reacted with N. caninum antigen also. Western blot revealed typical binding pattern according to ELISA values, such that high OD group reacted specifically to the major surface proteins including 43 kDa protein. Seroprevalence of 23.0% indicates that neosporosis seemed to be one of major causes of abortion in cattle. It is suggested here to establish more epidemiological researches nationwide systematically. Citations Citations to this article as recorded by Serological evaluation of Neospora caninum in pregnant women treated at referral center for prenatal screening in Mato Grosso do Sul, Brazil Pâmella Oliveira Duarte, Bárbara Guimarães Csordas, Leandra Marla Oshiro, Leandro de Oliveira Souza Higa, Namor Pinheiro Zimmermann, Kauê Rodriguez Martins, Jacqueline Cavalcante Barros, Renato Andreotti Revista Brasileira de Parasitologia Veterinária.2020;[Epub] CrossRef Meta-analysis of the prevalence and risk factors associated with bovine neosporosis Claudia Mello Ribeiro, Isabela Ribeiro Soares, Rodrigo Guerrero Mendes, Paula Andrea de Santis Bastos, Satie Katagiri, Renato Bacarin Zavilenski, Hudson Felipe Porto de Abreu, Vera Afreixo Tropical Animal Health and Production.2019; 51(7): 1783. CrossRef Seroprevalence of Neospora caninum infection in dairy cows in Northern provinces, Thailand Tawin Inpankaew, Sathaporn Jittapalapong, Thomas Mitchell, Chainirun Sununta, Ikuo Igarashi, Xuenan Xuan Acta Parasitologica.2014;[Epub] CrossRef Risk factors of Neospora caninum infection in dogs and cats in dairy farms in Western Thailand Pipat Arunvipas, Tawin Inpankaew, Sathaporn Jittapalapong Tropical Animal Health and Production.2012; 44(5): 1117. CrossRef Seroprevalence of Toxoplasma gondii and Neospora caninum in dogs from Korea Thuy Nguyen, Se-Eun Choe, Jae-Won Byun, Hong-Bum Koh, Hee-Soo Lee, Seung-Won Kang Acta Parasitologica.2012;[Epub] CrossRef Seroprevalence of Neospora caninum Antibodies in Cattle in Veracruz, Mexico D. Romero-Sal, Z. Garcia-Vaz, F. Montiel-Pa, T. Montiel-Pe, M. Aguilar-Do, L. Medina-Esp, C. Cruz-Vazqu Journal of Animal and Veterinary Advances.2010; 9(10): 1445. CrossRef Seroepidemiology of Neospora caninum and Toxoplasma gondii infection in yaks (Bos grunniens) in Qinghai, China J. Liu, J.Z. Cai, W. Zhang, Q. Liu, D. Chen, J.P. Han, Q.R. Liu Veterinary Parasitology.2008; 152(3-4): 330. CrossRef Seroepidemiology of Neospora caninum and Toxoplasma gondii in cattle and water buffaloes (Bubalus bubalis) in the People's Republic of China Jinhai Yu, Zhaofei Xia, Qun Liu, Jing Liu, Jun Ding, Wei Zhang Veterinary Parasitology.2007; 143(1): 79. CrossRef Epidemiology and Control of Neosporosis andNeospora caninum J. P. Dubey, G. Schares, L. M. Ortega-Mora Clinical Microbiology Reviews.2007; 20(2): 323. CrossRef Diagnosis of bovine neosporosis J.P. Dubey, G. Schares Veterinary Parasitology.2006; 140(1-2): 1. CrossRef ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera Hye-Jin Ahn, Sera Kim, Dae-Yong Kim, Ho-Woo Nam The Korean Journal of Parasitology.2003; 41(3): 175. CrossRef Seroprevalence of Neospora, Toxoplasma gondii and Sarcocystis neurona antibodies in horses from Jeju island, South Korea G.D Gupta, J Lakritz, Jae-Hoon Kim, Dae-Yong Kim, Jin-Kap Kim, A.E Marsh Veterinary Parasitology.2002; 106(3): 193. CrossRef Determination of antigenic domain in GST fused major surface protein (Nc-p43) of Neospora caninum Eui-Sun Son, Hye-Jin Ahn, Jae-Hoon Kim, Dae-Yong Kim, Ho-Woo Nam The Korean Journal of Parasitology.2001; 39(3): 241. CrossRef 10,499 View 88 Download Crossref