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Doaa M. Sultan	1 Article
Imported Malaria in United Arab Emirates: Evaluation of a New DNA Extraction Technique Using Nested PCR Doaa M. Sultan, Marwa M. Khalil, Ahmed S. Abdouh, Wafaa F. Doleh, Abdul Aziz M. Al Muthanna Korean J Parasitol 2009;47(3):227-233. Published online August 28, 2009 DOI: https://doi.org/10.3347/kjp.2009.47.3.227 Abstract PDF Local malaria transmission in the United Arab Emirates (UAE) came to an end in 1997. Nevertheless, UAE has been subjected to substantial importation of malaria cases from abroad, concerning both UAE nationals and immigrants from malarious countries with a total number of 2,119 cases in 2007. To evaluate a new DNA extraction technique using nested PCR, blood samples were collected from 132 individuals who presented to Infectious Diseases Department in Rashid Hospital, Dubai, and Central Department of Malaria Control with fever and persistent headache. Giemsa-stained blood films and ELISA test for malaria antibodies were carried out for detection of Plasmodium infection. Plasmodium infections were identified with the genus-specific primer set and species differentiation using nested PCR. A rapid procedure for diagnosis of malaria infections directly from dried blood spots using for the first time DNA extract from FTA Elute cards was evaluated in contrast to extraction techniques using FTA classic cards and rapid boiling technique. Our new simple technique for DNA extraction using FTA Elute cards was very sensitive giving a sensitivity of 100% compared to 94% using FTA classic cards and 62% in the rapid boiling technique. No complex preparation of blood samples was required prior to the amplification. The production cost of DNA isolation in our PCR assay was much less in comparable to that of other DNA extraction protocols. The nested PCR detected plasmodial infection and could differentiate P. falciparum from P. vivax, and also detected the mixed infection. Citations Citations to this article as recorded by Nucleic acid purification from dried blood spot on FTA Elute Card provides template for polymerase chain reaction for highly sensitive Plasmodium detection Muneaki Hashimoto, Mika Bando, Jun-ichi Kido, Kazumichi Yokota, Toshihiro Mita, Kazuaki Kajimoto, Masatoshi Kataoka Parasitology International.2019; 73: 101941. CrossRef Genetic diversity and transmissibility of imported Plasmodium vivax in Qatar and three countries of origin Mohammed H. Abdelraheem, Devendra Bansal, Mohammed A. Idris, Moawia M. Mukhtar, Muzamil M. Abdel Hamid, Zainb S. Imam, Sisay Getachew, Rakesh Sehgal, Hargobinder Kaur, Amal H. Gadalla, Salam Al-Hamidhi, Zainab Al-Hashami, Ali Al-Jabri, Ali A. Sultan, Hamz Scientific Reports.2018;[Epub] CrossRef A simple, efficient and inexpensive method for malaria parasites’ DNA catching from fixed Giemsa-stained blood slides Abbas Ali Eskandarian, Sara Moradi, Saeed Abedi Journal of Parasitic Diseases.2016; 40(3): 833. CrossRef Development of new malaria diagnostics: matching performance and need David Bell, Alessandra E. Fleurent, Michael C. Hegg, John D. Boomgard, Caitlin C. McConnico Malaria Journal.2016;[Epub] CrossRef Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination E. C. Oriero, J. Jacobs, J.-P. Van Geertruyden, D. Nwakanma, U. D'Alessandro Journal of Antimicrobial Chemotherapy.2015; 70(1): 2. CrossRef Genetic diversity of Plasmodium falciparum and distribution of drug resistance haplotypes in Yemen Salama Al-Hamidhi, Mohammed AK Mahdy, Zainab Al-Hashami, Hissa Al-Farsi, Abdulsalam M Al-mekhlafi, Mohamed A Idris, Albano Beja-Pereira, Hamza A Babiker Malaria Journal.2013;[Epub] CrossRef Quantitative detection of <i>Streptococcus mutans</i> from saliva using FTA<sup>TM</sup> elute cards and real-time polymerase chain reaction Sepideh Seghatoleslami, Lars Ohlsson, Kristina Hamberg, Peter Carlsson, Dan Ericson, Lennart Ljunggren American Journal of Molecular Biology.2013; 03(03): 148. CrossRef An optimized method for elution of enteroviral RNA from a cellulose-based substrate Yan Li, Hiromu Yoshida, Lu Wang, Zexin Tao, Haiyan Wang, Xiaojuan Lin, Aiqiang Xu Journal of Virological Methods.2012; 186(1-2): 62. CrossRef Comparative Analytical Utility of DNA Derived from Alternative Human Specimens for Molecular Autopsy and Diagnostics Tara L. Klassen, Eva-Lotta von Rüden, Janice Drabek, Jeffrey L. Noebels, Alica M. Goldman The Journal of Molecular Diagnostics.2012; 14(5): 451. CrossRef Source of drug resistant Plasmodium falciparum in a potential malaria elimination site in Saudi Arabia Hissa M. Al-Farsi, Zainab S. Al-Hashami, Saad M. Bin Dajem, Adel Ali H. Al-Sheikh, Ahmed Al-Qahtani, Albano Beja-Pereira, Mohamed A. Idris, Hamza A. Babiker Infection, Genetics and Evolution.2012; 12(6): 1253. CrossRef 9,680 View 84 Download Crossref