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Fatemeh Mansouri	1 Article
Production and Evaluation of Toxoplasma gondii Recombinant GRA7 for Serodiagnosis of Human Infections Mina Selseleh, Hossein Keshavarz, Mehdi Mohebali, Saeedeh Shojaee, Monavar Selseleh, Mohammad Reza Eshragian, Fatemeh Mansouri, Mohammad Hossein Modarressi Korean J Parasitol 2012;50(3):233-238. Published online August 13, 2012 DOI: https://doi.org/10.3347/kjp.2012.50.3.233 Abstract PDF The precise diagnosis of the acute toxoplasmosis in pregnant women and immunocompromsied patients has critical importance. Most of the commercially available assays use the whole Toxoplasma soluble extract as the antigen. However, the assays currently available for the detection of specific anti-Toxoplasma antibodies may vary in their abilities to detect serum immunoglobulins, due to the lack of a purified standardized antigen. The aim of this study was production and evaluation of the usefulness of the recombinant Toxoplasma gondii GRA7 antigen for the serodiagnosis of Toxoplasma gondii IgM and IgG by ELISA. A total of 70 T. gondii IgM positive sera, 74 T. gondii IgG positive sera, and 60 sera from subjects who were not infected with T. gondii were examined. These sera were shown different absorbance values in ELISA test. To control the specificity of the rGRA7 other parasitic diseases, for example, echinococcosis, malaria, leishmaniasis, fascioliasis, and strongyloidiasis were tested of which none showed positive results. Sensitivity and specificity of the generated recombinant IgG ELISA in comparison with commercial ELISA (com ELISA) were 89% and 90%, and the sensitivity and specificity of the generated recombinant IgM ELISA were 96% and 90%, respectively. The results obtained here show that this antigen is useful for diagnostic purposes. Citations Citations to this article as recorded by A Systematic Review and Meta-Analysis of Diagnostic Test Accuracy for Human Toxoplasmosis: Performance, Populations, and Validation Gaps Dodit Suprianto, Loeki Enggar Fitri, Akhmad Sabarudin, Wayan Mahmudy, Muhammad Prabowo, Werasak Surareungchai Medical Devices: Evidence and Research.2026; Volume 19: 1. CrossRef IgM Antibody Detection as a Diagnostic Marker for Acute Toxoplasmosis: Current Status of Studies and Main Limitations Karolina Sołowińska, Lucyna Holec-Gąsior Antibodies.2025; 14(2): 44. CrossRef Design of a Multi-epitope Antigen for Toxoplasmosis Diagnosis: An Immunoinformatics Approach Negar Asadi, Elham Yousefi, Sadegh Feizollahzadeh, Mortaza Taheri-Anganeh, Shahram Khademvatan, Gordon S. 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CrossRef Utility of blood as the clinical specimen for the diagnosis of ocular toxoplasmosis using uracil DNA glycosylase-supplemented loop-mediated isothermal amplification and real-time polymerase chain reaction assays based on REP-529 sequence and B1 gene Bahman Rahimi Esboei, Shirzad Fallahi, Mohammad Zarei, Bahram Kazemi, Mehdi Mohebali, Saeedeh Shojaee, Parisa Mousavi, Aref Teimouri, Raziyeh Mahmoudzadeh, Mirataollah Salabati, Hossein Keshavarz Valian BMC Infectious Diseases.2022;[Epub] CrossRef Four Chemotherapeutic Compounds That Limit Blood-Brain-Barrier Invasion by Toxoplasma gondii Zijing Yan, Hao Yuan, Junjie Wang, Zipeng Yang, Pian Zhang, Yasser S. Mahmmod, Xiaohu Wang, Tanghui Liu, Yining Song, Zhaowen Ren, Xiu-Xiang Zhang, Zi-Guo Yuan Molecules.2022; 27(17): 5572. CrossRef Assessment of an In-House Enzyme-Linked Immunosorbent Assay and IgG Avidity Test Based on SAG1 and GRA7 Proteins for Discriminating between Acute and Chronic Toxoplasmosis in Humans Aref Teimouri, Mohammad Javad Abbaszadeh Afshar, Sina Mohtasebi, Sanaz Jafarpour Azami, Rasoul Alimi, Hossein Keshavarz, Bobbi S. Pritt Journal of Clinical Microbiology.2021;[Epub] CrossRef Enhancing Immune Responses to a DNA Vaccine Encoding Toxoplasma gondii GRA7 Using Calcium Phosphate Nanoparticles as an Adjuvant Hong-Chao Sun, Jing Huang, Yuan Fu, Li-Li Hao, Xin Liu, Tuan-Yuan Shi Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef Designing Diagnostic Kit for Toxoplasma gondii Based on GRA7, SAG1, and ROP1 Antigens: An In Silico Strategy Amirreza Javadi Mamaghani, Seyyed Javad Seyyed Tabaei, Mohammad Mehdi Ranjbar, Ali Haghighi, Adel Spotin, Parisa Ataee Dizaji, Hanieh Rezaee International Journal of Peptide Research and Therapeutics.2020; 26(4): 2269. CrossRef Serological detection of T. gondii infection in humans using an immunochromatographic assay based on dense granule protein 7 Rochelle Haidee D. Ybañez, Yoshifumi Nishikawa Parasitology International.2020; 76: 102089. CrossRef *Detection of antibodies against Toxoplasma gondii* in cats using an immunochromatographic test based on GRA7 antigen** Rochelle Haidee D. YBAÑEZ, Hisako KYAN, Yoshifumi NISHIKAWA Journal of Veterinary Medical Science.2020; 82(4): 441. CrossRef Review on the Current Trends of Toxoplasmosis Serodiagnosis in Humans Rochelle Haidee D. Ybañez, Adrian P. Ybañez, Yoshifumi Nishikawa Frontiers in Cellular and Infection Microbiology.2020;[Epub] CrossRef Identification of novel antigens for serum IgG diagnosis of human toxoplasmosis Juntao Luo, Jingyi Wan, Ziru Tang, Shuang Shen Experimental Parasitology.2019; 204: 107722. CrossRef Candidate antigenic epitopes for vaccination and diagnosis strategies of Toxoplasma gondii infection: A review Amirreza Javadi Mamaghani, Anwar Fathollahi, Adel Spotin, Mohammad mehdi Ranjbar, Meisam Barati, Somayeh Aghamolaie, Maryam Karimi, Niloofar Taghipour, Mohammad Ashrafi, Seyyed Javad Seyyed Tabaei Microbial Pathogenesis.2019; 137: 103788. CrossRef Advances in serological, imaging techniques and molecular diagnosis of Toxoplasma gondii infection Ali Rostami, Panagiotis Karanis, Shirzad Fallahi Infection.2018; 46(3): 303. CrossRef Antigens to Detect the Acute Phase of Toxoplasmosis in Pregnant Women: Standardized Comparison Juan Gabriel Costa, María Julia Vilariño Biomarkers in Medicine.2018; 12(5): 517. CrossRef Detection of toxoplasma-specific immunoglobulin G in human sera: performance comparison of in house Dot-ELISA with ECLIA and ELISA Aref Teimouri, Mohammad Hossein Modarressi, Saeedeh Shojaee, Mehdi Mohebali, Nima Zouei, Mostafa Rezaian, Hossein Keshavarz European Journal of Clinical Microbiology & Infectious Diseases.2018; 37(8): 1421. CrossRef The association of latent toxoplasmosis and level of serum testosterone in humans Nima Zouei, Saeedeh Shojaee, Mehdi Mohebali, Hossein Keshavarz BMC Research Notes.2018;[Epub] CrossRef The relation of secondary sex ratio and miscarriage history with Toxoplasma gondii infection Saeedeh Shojaee, Aref Teimouri, Hossein Keshavarz, Sanaz Jafarpour Azami, Sahar Nouri BMC Infectious Diseases.2018;[Epub] CrossRef Seroprevalence of Toxoplasma gondii and Neospora caninum infections in cattle in Mongolia Baldorj Pagmadulam, Punsantsogvoo Myagmarsuren, Ragab M. Fereig, Makoto Igarashi, Naoaki Yokoyama, Badgar Battsetseg, Yoshifumi Nishikawa Veterinary Parasitology: Regional Studies and Reports.2018; 14: 11. CrossRef Early detection of Toxoplasma gondii infection by using a interferon gamma release assay: A review Shima Mahmoudi, Setareh Mamishi, Xun Suo, Hossein Keshavarz Experimental Parasitology.2017; 172: 39. CrossRef Toxoplasma gondii GRA7-Targeted ASC and PLD1 Promote Antibacterial Host Defense via PKCα Hyun-Jung Koh, Ye-Ram Kim, Jae-Sung Kim, Jin-Seung Yun, Kiseok Jang, Chul-Su Yang, Sarah M. Fortune PLOS Pathogens.2017; 13(1): e1006126. CrossRef Dense Granule Protein-7 (GRA-7) of Toxoplasma gondii inhibits viral replication in vitro and in vivo Prasanna Weeratunga, Thilina U. B. Herath, Tae-Hwan Kim, Hyun-Cheol Lee, Jae-Hoon Kim, Byeong-Hoon Lee, Eun-Seo Lee, Kiramage Chathuranga, W. A. Gayan Chathuranga, Chul-Su Yang, Jin Yeul Ma, Jong-Soo Lee Journal of Microbiology.2017; 55(11): 909. CrossRef Cloning and Sequencing of Truncated Toxoplasma gondii Subtilisin-Like 1 Antigen Ahmad Rouhizadeh, Ata A Ghadiri, Mohammad Razi Jalali, Masoud Ghorbanpour, Mohammad Hossein Razi Jalali Zahedan Journal of Research in Medical Sciences.2016;[Epub] CrossRef Toxoplasma gondii GRA7-Induced TRAF6 Activation Contributes to Host Protective Immunity Chul-Su Yang, Jae-Min Yuk, Young-Ha Lee, Eun-Kyeong Jo, J. H. Adams Infection and Immunity.2016; 84(1): 339. CrossRef Production and Evaluation of Recombinant Granule Antigen Protein GRA7 for Serodiagnosis ofToxoplasma gondiiInfection in Cattle Zedong Wang, Wei Ge, Jiping Li, Mingxing Song, Hongchao Sun, Feng Wei, Quan Liu Foodborne Pathogens and Disease.2014; 11(9): 734. CrossRef Toxoplasma gondii infection: What is the real situation? Waenurama Chemoh, Nongyao Sawangjaroen, Veeranoot Nissapatorn, Chitkasaem Suwanrath, Verapol Chandeying, Thanaporn Hortiwakul, Hemah Andiappan, Natthawan Sermwittayawong, Bunsri Charoenmak, Pisut Siripaitoon, Amorn Lekkla, Yaowalark Sukthana Experimental Parasitology.2013; 135(4): 685. CrossRef Toxoplasma gondii Recombinant Antigens as Tools for Serodiagnosis of Human Toxoplasmosis: Current Status of Studies Lucyna Holec-Gąsior Clinical and Vaccine Immunology.2013; 20(9): 1343. CrossRef 9,507 View 95 Download Crossref