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Hee Jae Cha	4 Articles
Expression and Characterization of α-Methylacyl CoA Racemase from Anisakis simplex Larvae Bong Jin Kim, Sun Mi Kim, Min Kyung Cho, Hak Sun Yu, Yong Seok Lee, Hee Jae Cha, Meesun Ock Korean J Parasitol 2012;50(2):165-171. Published online May 24, 2012 DOI: https://doi.org/10.3347/kjp.2012.50.2.165 Abstract PDF Larval excretory-secretory products of Anisakis simplex are known to cause allergic reactions in humans. A cDNA library of A. simplex 3rd-stage larvae (L3) was immunoscreened with polyclonal rabbit serum raised against A. simplex L3 excretory-secretory products to identify an antigen that elicits the immune response. One cDNA clone, designated as α-methylacyl CoA racemase (Amacr) contained a 1,412 bp cDNA transcript with a single open reading frame that encoded 418 amino acids. A. simplex Amacr showed a high degree of homology compared to Amacr orthologs from other species. Amacr mRNA was highly and constitutively expressed regardless of temperature (10-40℃) and time (24-48 hr). Immunohistochemical analysis revealed that Amacr was expressed mainly in the ventriculus of A. simplex larvae. The Amacr protein produced in large quantities from the ventriculus is probably responsible for many functions in the development and growth of A. simplex larvae. Citations Citations to this article as recorded by The mitochondrial genome ofAncylostoma tubaeformefrom cats in China X.L. Shi, Y.Q. Fu, A.Y. Abdullahi, M.W. Wang, F. Yang, X.G. Yu, W.D. Pan, X.X. Yan, J.X. Hang, P. Zhang, G.Q. Li Journal of Helminthology.2018; 92(1): 22. CrossRef α-Methylacyl-CoA racemase (AMACR): Metabolic enzyme, drug metabolizer and cancer marker P504S Matthew D. Lloyd, Maksims Yevglevskis, Guat Ling Lee, Pauline J. Wood, Michael D. Threadgill, Timothy J. Woodman Progress in Lipid Research.2013; 52(2): 220. CrossRef 10,029 View 51 Download Crossref A 24 kDa Excretory-Secretory Protein of Anisakis simplex Larvae Could Elicit Allergic Airway Inflammation in Mice Hye-Kyung Park, Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Yun Seong Kim, Ki Uk Kim, Min Ki Lee, Mee Sun Ock, Hee Jae Cha, Hak Sun Yu Korean J Parasitol 2011;49(4):373-380. Published online December 16, 2011 DOI: https://doi.org/10.3347/kjp.2011.49.4.373 Abstract PDF We have reported that a 24 kDa protein (22U homologous; As22U) of Anisakis simplex larvae could elicit several Th2-related chemokine gene expressions in the intestinal epithelial cell line which means that As22U may play a role as an allergen. In order to determine the contribution of As22U to allergic reactions, we treated mice with 6 times intra-nasal application of recombinant As22U (rAs22U). In the group challenged with rAs22U and ovalbumin (OVA), the number of eosinophils in the bronchial alveolar lavage fluid (BALF) was significantly increased, as compared to the group receiving only OVA. In addition, mice treated with rAs22U and OVA showed significantly increased airway hyperresponsiveness. Thus, severe inflammation around the airway and immune cell recruitment was observed in mice treated with rAs22U plus OVA. The levels of IL-4, IL-5, and IL-13 cytokines in the BALF increased significantly after treatment with rAs22U and OVA. Similarly, the levels of anti-OVA specific IgE and IgG1 increased in mice treated with rAs22U and OVA, compared to those treated only with OVA. The Gro-α (CXCL1) gene expression in mouse lung epithelial cells increased instantly after treatment with rAs22U, and allergy-specific chemokines eotaxin (CCL11) and thymus-and-activation-regulated-chemokine (CCL17) gene expressions significantly increased at 6 hr after treatment. In conclusion, rAs22U may induce airway allergic inflammation, as the result of enhanced Th2 and Th17 responses. Citations Citations to this article as recorded by Anisakis pegreffii Extract Induces Airway Inflammation with Airway Remodeling in a Murine Model System Jun Ho Choi, Ju Yeong Kim, Myung-hee Yi, Myungjun Kim, Tai-Soon Yong, Kalman Imre BioMed Research International.2021;[Epub] CrossRef Allergen-like Molecules from Parasites Ju Yeong Kim, Myung-Hee Yi, Tai-Soon Yong Current Protein & Peptide Science.2020; 21(2): 186. CrossRef Research Note. Prevalence, protein analysis and possible preventive measures against zoonotic anisakid larvae isolated from marine Atherina fish M. Samir, M. A. Amin, A. O. Hassan, A. M. Merwad, M. A. I. Awadallah Helminthologia.2015; 52(4): 375. CrossRef Allergenicity of two Anisakis simplex allergens evaluated in vivo using an experimental mouse model Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Maria Luisa Caballero, Teresa Perez-Pinar, Rosa Rodriguez-Perez, Mee Sun Ock, Hee Jae Cha, Yeon Chul Hong, Hak Sun Yu Experimental Parasitology.2014; 146: 71. CrossRef Alteration of Cytokine Production during Visceral Larva Migrans by Toxascaris leonina in Mice Shin Ae Kang, Mi-Kyung Park, Min Kyoung Cho, Hak Sun Yu The Korean Journal of Parasitology.2013; 51(5): 583. CrossRef 9,530 View 76 Download Crossref Seroprevalence of Antibodies against Anisakis simplex Larvae among Health-Examined Residents in Three Hospitals of Southern Parts of Korea Jung Kim, Jin Ok Jo, Seon Hee Choi, Min Kyoung Cho, Hak Sun Yu, Hee Jae Cha, Meesun Ock Korean J Parasitol 2011;49(2):139-144. Published online June 14, 2011 DOI: https://doi.org/10.3347/kjp.2011.49.2.139 Abstract PDF The present study was performed to estimate the seroprevalence of larval Anisakis simplex infection among the residents health-examined in 3 hospitals in southern parts of Korea. A total of 498 serum samples (1 serum per person) were collected in 3 hospitals in Busan Metropolitan city, Masan city, and Geoje city in Gyeongsangnam-do (Province) and were examined by IgE-ELISA and IgE-western blotting with larval A. simplex crude extract and excretory-secretory products (ESP). The prevalence of antibody positivity was 5.0% and 6.6% with ELISA against crude extracts and ESP, respectively. It was also revealed that infection occurred throughout all age groups and higher in females than in males. A specific protein band of 130 kDa was detected from 10 patients with western blot analysis against crude extract and ESP among those who showed positive results by ELISA. Our study showed for the first time the seroprevalence of anisakiasis in Korea. The allergen of 130 kDa can be a candidate for serologic diagnosis of anisakiasis. Citations Citations to this article as recorded by Seroprevalence of IgG and IgE Antibodies Against Anisakis in the Presumably Healthy Population of the Canary Islands Eligia González-Rodríguez, Marta Rodero, J. Alberto Montoya-Alonso, Kevin M. Santana-Hernández, Myriam R. Ventura, Carmen Cuéllar, Eligia Rodríguez-Ponce Antibodies.2025; 14(3): 60. CrossRef Proteome identification of common immunological proteins of two nematode parasites Shin Ae Kang, Hak Sun Yu Parasites, Hosts and Diseases.2024; 62(3): 342. CrossRef Progress in Anisakis Allergy Research: Milestones and Reversals Alvaro Daschner, Carmen Cuéllar Current Treatment Options in Allergy.2020; 7(4): 457. CrossRef IgE sensitization to Anisakis pegreffii in Italy: Comparison of two methods for the diagnosis of allergic anisakiasis S. Mattiucci, A. Colantoni, B. Crisafi, F. Mori‐Ubaldini, L. Caponi, P. Fazii, G. Nascetti, F. Bruschi Parasite Immunology.2017;[Epub] CrossRef Anthelmintic Activities of Aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana Rong-Jyh Lin, Mei-Hsuan Wu, Yi-Hsuan Ma, Li-Yu Chung, Chung-Yi Chen, Chuan-Min Yen International Journal of Molecular Sciences.2014; 15(3): 3624. CrossRef Endoscopic imaging of parasites in the human digestive tract Naoki Hosoe, Haruhiko Ogata, Toshifumi Hibi Parasitology International.2014; 63(1): 216. CrossRef An Extended Study of Seroprevalence of Anti‐Anisakis simplex IgE Antibodies in Norwegian Blood Donors A. H. Lin, I. Nepstad, E. Florvaag, E. Egaas, T. Van Do Scandinavian Journal of Immunology.2014; 79(1): 61. CrossRef Occurrence of anisakid nematode larvae in chub mackerel (Scomber japonicus) caught off Korea Tae-Jong Bak, Chan-Hyeok Jeon, Jeong-Ho Kim International Journal of Food Microbiology.2014; 191: 149. CrossRef Clinical Characteristics of Gastroallergic Anisakiasis and Diagnostic Implications of Immunologic Tests Young-Bae Chung, Jaechun Lee Allergy, Asthma & Immunology Research.2014; 6(3): 228. CrossRef Preventive and Therapeutic Effects of <italic>Anisakis simplex</italic> Larval Protein in a Mouse Model of Crohn’S Disease Hee-Jae Cha, Mee Sun Ock Kosin Medical Journal.2013; 28(2): 107. CrossRef Cross-sectional study of serum reactivity to Anisakis simplex in healthy adults in Niter?i, Brazil Israel Junior, Mauricio Vericimo, Luciana Cardoso, Sergio Clemente, Elmiro Nascimento, Gerlinde Teixeira Acta Parasitologica.2013;[Epub] CrossRef Detection of Microorganisms in Granulomas That Have Been Formalin-Fixed: Review of the Literature Regarding Use of Molecular Methods Jeannette Guarner Scientifica.2012; 2012: 1. CrossRef Anisakidosis in Korea; Changes Over the Last Decade Hee Jae Cha, Mee Sun Ock Kosin Medical Journal.2012; 27(2): 73. CrossRef Prevalence of Zoonotic Anisakid Nematodes in Inuit-Harvested Fish and Mammals from the Eastern Canadian Arctic Erica L. Pufall, Andria Jones-Bitton, Scott A. McEwen, Tanya M. Brown, Victoria L. Edge, Jerzy Rokicki, Katarzyna Karpiej, Andrew S. Peregrine, Manon Simard Foodborne Pathogens and Disease.2012; 9(11): 1002. CrossRef A technique for the intra-gastric administration of live larvae of Anisakis simplex in mice Israel Figueiredo, Luciana Cardoso, Gerlinde Teixeira, Leila Lopes, Sergio Carmona São Clemente, Mauricio Afonso Vericimo Experimental Parasitology.2012; 130(3): 285. CrossRef 10,101 View 92 Download Crossref Anisakis simplex Larvae: Infection Status in Marine Fish and Cephalopods Purchased from the Cooperative Fish Market in Busan, Korea Seon Hee Choi, Jung Kim, Jin Ok Jo, Min Kyung Cho, Hak Sun Yu, Hee Jae Cha, Mee Sun Ock Korean J Parasitol 2011;49(1):39-44. Published online March 18, 2011 DOI: https://doi.org/10.3347/kjp.2011.49.1.39 Abstract PDF The infection status of marine fish and cephalopods with Anisakis simplex third stage larva (L3) was studied over a period of 1 year. A total of 2,537 specimens, which consisted of 40 species of fish and 3 species of cephalopods, were purchased from the Cooperative Fish Market in Busan, Korea, from August 2006 to July 2007. They were examined for A. simplex L3 from the whole body cavity, viscera, and muscles. A. simplex L3 were confirmed by light microscopy. The overall infection rate reached 34.3%, and average 17.1 larvae were parasitized per infected fish. Fish that recorded the highest infection rate was Lophiomus setigerus (100%), followed by Liparis tessellates (90%), Pleurogrammus azonus (90%), and Scomber japonicus (88.7%). The intensity of infection was the highest in Gadus macrocephalus (117.7 larvae per fish), followed by S. japonicus (103.9 larvae) and L. setigerus (54.2 larvae). Although abundance of A. simplex L3 was not seasonal in most of the fish species, 10 of the 16 selected species showed the highest abundance in February and April. A positive correlation between the intensity of L3 infection and the fish length was obvious in S. japonicus and G. macrocephalus. It was likely that A. simplex L3 are more frequently infected during the spring season in some species of fish. Our study revealed that eating raw or undercooked fish or cephalopods could still be a source of human infection with A. simplex L3 in Korea. Citations Citations to this article as recorded by Comparative study of Taqman-based qPCR assay for the detection of Anisakis simplex and Pseudoterranova decipiens Mi-Gyeong Kim, Min Ji Hong, Doo Won Seo, Hyun Mi Jung, Hyun-Ja Han, Seung Hwan Kim, Insun Joo, Elingarami Sauli PLOS One.2025; 20(4): e0320724. CrossRef A critical review of anisakidosis cases occurring globally Shokoofeh Shamsi, Diane P. Barton Parasitology Research.2023; 122(8): 1733. CrossRef Larval ascaridoid nematodes in horned and musky octopus (Eledone cirrhosa and E. moschata) and longfin inshore squid (Doryteuthis pealeii): Safety and quality implications for cephalopod products sold as fresh on the Italian market L. Guardone, E. Bilska-Zając, A. Giusti, R. Malandra, T. Cencek, A. Armani International Journal of Food Microbiology.2020; 333: 108812. CrossRef Assessment of parasite virulence in a natural population of a planktonic crustacean Eevi Savola, Dieter Ebert BMC Ecology.2019;[Epub] CrossRef Seafood-Borne Parasitic Diseases: A “One-Health” Approach Is Needed Shokoofeh Shamsi Fishes.2019; 4(1): 9. CrossRef Endosonographic Findings and the Natural Course of Chronic Gastric Anisakiasis: A Single-Center Experience Eun Young Park, Dong Hoon Baek, Gwang Ha Kim, Bong Eun Lee, So-Jeong Lee, Do Youn Park Gastroenterology Research and Practice.2018; 2018: 1. CrossRef Hiện trạng nhiễm ký sinh trùng trên cá bớp (Rachycentron canadum) nuôi lồng ở tỉnh Kiên Giang Từ Thanh Dung, Phan Văn Út, Nguyễn Bảo Trung Can Tho University Journal of Science.2017; 51: 106. CrossRef Occurrence and molecular identification of anisakid nematodes isolated from Pacific cod ( Gadus macrocephalus ) caught off Korea Chan-Hyeok Jeon, Eko Setyobudi, Jeong-Ho Kim Food Control.2016; 69: 100. CrossRef Inactivation of Anisakis simplex L3 in the flesh of white spotted conger (Conger myriaster) by high hydrostatic pressure and its effect on quality Ki-Hoon Lee, Shin Young Park, Sang-Do Ha Food Additives & Contaminants: Part A.2016; 33(6): 1010. CrossRef Simultaneous Detection and Prevalence of Allergens in Anisakis Species Isolated from Marine Fishes Woo Joo Lee, Dong Joo Seo, Hyejin Oh, Su Been Jeon, Day Jung, Changsun Choi Journal of Food Protection.2016; 79(5): 789. CrossRef <i>Anisakis pegreffii</i> Larvae in Sea Eels (<i>Astroconger myriaster</i>) from the South Sea, Republic of Korea Jaeeun Cho, Hyemi Lim, Bong-Kwang Jung, Eun-Hee Shin, Jong-Yil Chai The Korean Journal of Parasitology.2015; 53(3): 349. CrossRef Molecular Analysis ofAnisakis Type I Larvae in Marine Fish from Three Different Sea Areas in Korea Woon-Mok Sohn, Jung-Mi Kang, Byoung-Kuk Na The Korean Journal of Parasitology.2014; 52(4): 383. CrossRef Molecular identification of anisakid nematodes third stage larvae isolated from common squid (Todarodes pacificus) in Korea Eko Setyobudi, Chan-Hyeok Jeon, Kwangho Choi, Sung Il Lee, Chung Il Lee, Jeong-Ho Kim Ocean Science Journal.2013; 48(2): 197. CrossRef Anisakidosis in Korea; Changes Over the Last Decade Hee Jae Cha, Mee Sun Ock Kosin Medical Journal.2012; 27(2): 73. CrossRef Larval Anisakid Infections in Marine Fish from Three Sea Areas of the Republic of Korea Shin-Hyeong Cho, Sang-Eun Lee, Ok-Hee Park, Byoung-Kuk Na, Woon-Mok Sohn The Korean Journal of Parasitology.2012; 50(4): 295. CrossRef A rapid PCR–RFLP method for the identification of Lophius species Andrea Armani, Lorenzo Castigliego, Lara Tinacci, Gabriele Gandini, Daniela Gianfaldoni, Alessandra Guidi European Food Research and Technology.2012; 235(2): 253. CrossRef Laryngeal Anisakiasis Seo Young Kwak, Yeo‐Hoon Yoon Otolaryngology–Head and Neck Surgery.2012; 147(3): 588. CrossRef Seroprevalence of Antibodies againstAnisakis simplexLarvae among Health-Examined Residents in Three Hospitals of Southern Parts of Korea Jung Kim, Jin Ok Jo, Seon Hee Choi, Min Kyoung Cho, Hak Sun Yu, Hee Jae Cha, Meesun Ock The Korean Journal of Parasitology.2011; 49(2): 139. CrossRef 10,697 View 103 Download Crossref