Parasites, Hosts and Diseases

Skip to main navigation Skip to main content

OPEN ACCESS

Page Path

HOME
BROWSE ARTICLES

Author index detail contents
Hirohito Kita	2 Articles
A 27 kDa Cysteine Protease Secreted by Newly Excysted Paragonimus westermani Metacercariae Induces Superoxide Anion Production and Degranulation of Human Eosinophils Young-Bae Chung, Hirohito Kita, Myeong Heon Shin Korean J Parasitol 2008;46(2):95-99. Published online June 20, 2008 DOI: https://doi.org/10.3347/kjp.2008.46.2.95 Abstract PDF Eosinophil degranulation plays a crucial role in tissue inflammatory reactions associated with helminth parasitic infections and allergic diseases. Paragonimus westermani, a lung fluke causing human paragonimiasis, secretes a large amount of cysteine proteases, which are involved in nutrient uptake, tissue invasion, and modulation of hos's immune responses. There is, however, limited information about the response of eosinophils to direct stimulation by cysteine proteases (CP) secreted by P. westermani. In the present study, we tested whether degranulation and superoxide production from human eosinophils can be induced by stimulation of the 2 CP (27 kDa and 28 kDa) purified from excretory-secretory products (ESP) of P. westermani newly excysted metacercariae (PwNEM). A large quantity of eosinophil-derived neurotoxin (EDN) was detected in the culture supernatant when human eosinophils isolated from the peripheral blood were incubated with the purified 27 kDa CP. Furthermore, the 27 kDa CP induced superoxide anion production by eosinophils in time- and dose-dependent manners. In contrast, the purified 28 kDa CP did not induce superoxide production and degranulation. These findings suggest that the 27 kDa CP secreted by PwNEM induces superoxide production and degranulation of human eosinophils, which may be involved in eosinophil-mediated tissue inflammatory responses during the larval migration in human paragonimiasis. Citations Citations to this article as recorded by Massive secretions in paragonimiasis pleural effusion: a new finding concerning clinical recognition and treatment Yunxuan Zhang, Yimin Xie, Yarui Luo, Shuang Xiang, Wen Zhong, Na Wu, Meng Chen, Xiaojiao Zhou, Shulei Peng, Qiang Chen European Journal of Clinical Microbiology & Infectious Diseases.2023; 42(4): 493. CrossRef Transcriptome Analysis Reveals Possible Virulence Factors of Paragonimus proliferus Sheng-Hao Li, Shu-De Li, Kun-Li Wu, Jun-Yi Li, Hong-Juan Li, Wei-Qun Wang, Li-Jun Yang, Jing-Jing Xu, Guo-Ji Chang, Yan-Ling Zhang, Qiu-Hong Shu, Shan-Shan Zhuang, Zhi-Qiang Ma, Shu-Meiqi He, Min Zhu, Wen-Lin Wang, Hong-Li Huang Current Bioinformatics.2021; 16(2): 197. CrossRef Downregulated RPS-30 in Angiostrongylus cantonensis L5 plays a defensive role against damage due to oxidative stress Wei-Wei Sun, Xiu-Mei Yan, Qing Shi, Yuan-Jiao Zhang, Jun-Ting Huang, Hui-Cong Huang, Hong-Fei Shi, Bao-Long Yan Parasites & Vectors.2020;[Epub] CrossRef Molecular and immunological characterization of cathepsin L-like cysteine protease of Paragonimus pseudoheterotremus Tippayarat Yoonuan, Supaporn Nuamtanong, Paron Dekumyoy, Orawan Phuphisut, Poom Adisakwattana Parasitology Research.2016; 115(12): 4457. CrossRef Conservation and diversification of the transcriptomes of adult Paragonimus westermani and P. skrjabini Ben-wen Li, Samantha N. McNulty, Bruce A. Rosa, Rahul Tyagi, Qing Ren Zeng, Kong-zhen Gu, Gary J. Weil, Makedonka Mitreva Parasites & Vectors.2016;[Epub] CrossRef Systems Biology Studies of Adult Paragonimus Lung Flukes Facilitate the Identification of Immunodominant Parasite Antigens Samantha N. McNulty, Peter U. Fischer, R. Reid Townsend, Kurt C. Curtis, Gary J. Weil, Makedonka Mitreva, Aaron R. Jex PLoS Neglected Tropical Diseases.2014; 8(10): e3242. CrossRef Eosinophilic Pneumonias Praveen Akuthota, Peter F. Weller Clinical Microbiology Reviews.2012; 25(4): 649. CrossRef Eosinophil and Tissue-invasive Parasitic Helminth Myeong Heon Shin Hanyang Medical Reviews.2010; 30(3): 238. CrossRef Eosinophil-Mediated Tissue Inflammatory Responses in Helminth Infection Myeong Heon Shin, Young Ah Lee, Duk-Young Min The Korean Journal of Parasitology.2009; 47(Suppl): S125. CrossRef The global cysteine peptidase landscape in parasites Holly J. Atkinson, Patricia C. Babbitt, Mohammed Sajid Trends in Parasitology.2009; 25(12): 573. CrossRef Parasitic helminths: a pharmacopeia of anti-inflammatory molecules M. J. G. JOHNSTON, J. A. MacDONALD, D. M. McKAY Parasitology.2009; 136(2): 125. CrossRef 8,762 View 76 Download Crossref Degranulation of human eosinophils induced by Paragonimus westermani-secreted protease Myeong Heon Shin, Young-Bae Chung, Hirohito Kita Korean J Parasitol 2005;43(1):33-37. Published online March 20, 2005 DOI: https://doi.org/10.3347/kjp.2005.43.1.33 Abstract PDF Eosinophil degranulation is considered to be a key effector function for the killing of helminthic worms and tissue inflammation at worm-infected lesion sites. However, relatively little data are available with regard to eosinophil response after stimulation with worm-secreted products which contain a large quantity of cysteine proteases. In this study, we attempted to determine whether the degranulation of human eosinophils could be induced by the direct stimulation of the excretory-secretory products (ESP) of Paragonimus westermani, which causes pulmonary paragonimiasis in human beings. Incubation of eosinophils for 3 hr with Paragonimus-secreted products resulted in marked degranulation, as evidenced by the release of eosinophil-derived neurotoxin (EDN) in the culture supernatants. Moreover, superoxide anion was produced by eosinophils after stimulation of the ESP. The ESP-induced EDN release was found to be significantly inhibited when the ESP was pretreated with protease inhibitor cocktail or the cysteine protease inhibitor, E-64. These findings suggest that human eosinophils become degranulated in response to P. westermani-secreted proteases, which may contribute to in vivo tissue inflammation around the worms. Citations Citations to this article as recorded by Interface Molecules ofAngiostrongylus cantonensis: Their Role in Parasite Survival and Modulation of Host Defenses Alessandra L. Morassutti, Carlos Graeff-Teixeira International Journal of Inflammation.2012; 2012: 1. CrossRef Echinococcus multilocularis: Identification and functional characterization of cathepsin B-like peptidases from metacestode Yasuhito Sako, Kazuhiro Nakaya, Akira Ito Experimental Parasitology.2011; 127(3): 693. CrossRef Eosinophil and Tissue-invasive Parasitic Helminth Myeong Heon Shin Hanyang Medical Reviews.2010; 30(3): 238. CrossRef Eosinophil-Mediated Tissue Inflammatory Responses in Helminth Infection Myeong Heon Shin, Young Ah Lee, Duk-Young Min The Korean Journal of Parasitology.2009; 47(Suppl): S125. CrossRef A 27 kDa Cysteine Protease Secreted by Newly Excysted Paragonimus westermani Metacercariae Induces Superoxide Anion Production and Degranulation of Human Eosinophils Young-Bae Chung, Hirohito Kita, Myeong Heon Shin The Korean Journal of Parasitology.2008; 46(2): 95. CrossRef Cloning and characterization of cathepsin L-like peptidases of Echinococcus multilocularis metacestodes Yasuhito Sako, Hiroshi Yamasaki, Kazuhiro Nakaya, Minoru Nakao, Akira Ito Molecular and Biochemical Parasitology.2007; 154(2): 181. CrossRef Identification of immunodominant excretory–secretory cysteine proteases of adult Paragonimus westermani by proteome analysis Eung‐Goo Lee, Byoung‐Kuk Na, Young‐An Bae, Seon‐Hee Kim, Eun‐Young Je, Jeong‐Won Ju, Shin‐Hyeong Cho, Tong‐Soo Kim, Shin‐Yong Kang, Seung‐Yull Cho, Yoon Kong PROTEOMICS.2006; 6(4): 1290. CrossRef Partial characterization of a 29 kDa cysteine protease purified from Taenia solium metacestodes Ji-Young Kim, Hyun-Jong Yang, Kwang-Sig Kim, Young-Bae Chung The Korean Journal of Parasitology.2005; 43(4): 157. CrossRef 9,275 View 69 Download Crossref