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Ho-Cheol Yun	2 Articles
Isolation and characterization of a cDNA encoding a mammalian cathepsin L-like cysteine proteinase from Acanthamoeba healyi Yeon-Chul Hong, Mi-Yul Hwang, Ho-Cheol Yun, Hak-Sun Yu, Hyun-Hee Kong, Tai-Soon Yong, Dong-Il Chung Korean J Parasitol 2002;40(1):17-24. Published online March 31, 2002 DOI: https://doi.org/10.3347/kjp.2002.40.1.17 Abstract PDF We have cloned a cDNA encoding a cysteine proteinase of the Acanthamoeba healyi OC-3A strain isolated from the brain of a granulomatous amoebic encephalitis patient. A DNA probe for an A. healyi cDNA library screening was amplified by PCR using degenerate oligonucleotide primers designed on the basis of conserved amino acids franking the active sites of cysteine and asparagine residues that are conserved in the eukaryotic cysteine proteinases. Cysteine proteinase gene of A. healyi (AhCP1) was composed of 330 amino acids with signal sequence, a proposed pro-domain and a predicted active site made up of the catalytic residues, Cys²⁵, His¹⁵⁹, and Asn¹⁷⁵. Deduced amino acid sequence analysis indicates that AhCP1 belong to ERFNIN subfamily of C1 peptidases. By Northern blot analysis, no direct correlation was observed between AhCP1 mRNA expression and virulence of Acanthamoeba, but the gene was expressed at higher level in amoebae isolated from soil than amoeba from clinical samples. These findings raise the possibility that Ahcp1 protein may play a role in protein metabolism and digestion of phagocytosed bacteria or host tissue debris rather than in invasion of amoebae into host tissue. Citations Citations to this article as recorded by Amoebic encephalitis within Australia Samantha C. Carija, Avram Levy, Graham Weaire‐Buchanan, Terence Lee, Robin Woodward, Jake Gazeley, Troy A. Edwards, Jason M. Dyke, Vicki Fabian, Katherine Norton, Andrew Chapman, Paul R. Ingram Internal Medicine Journal.2025; 55(1): 143. CrossRef Biological characteristics and pathogenicity of Acanthamoeba Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li Frontiers in Microbiology.2023;[Epub] CrossRef Identification and biochemical characterisation of Acanthamoeba castellanii cysteine protease 3 Zhixin Wang, Duo Wu, Hiroshi Tachibana, Meng Feng, Xun-jia Cheng Parasites & Vectors.2020;[Epub] CrossRef Anti-amoebic potential of azole scaffolds and nanoparticles against pathogenic Acanthamoeba Shweta Walvekar, Ayaz Anwar, Areeba Anwar, Nanthini Sridewi, Mohammad Khalid, Yoon Yen Yow, Naveed Ahmed Khan Acta Tropica.2020; 211: 105618. CrossRef Host Invasion by Pathogenic Amoebae: Epithelial Disruption by Parasite Proteins Abigail Betanzos, Cecilia Bañuelos, Esther Orozco Genes.2019; 10(8): 618. CrossRef Molecular and Biochemical Properties of a Cysteine Protease of Acanthamoeba castellanii Yeonchul Hong, Jung-Mi Kang, So-Young Joo, Su-Min Song, Hương Giang Lê, Thị Lam Thái, Jinyoung Lee, Youn-Kyoung Goo, Dong-Il Chung, Woon-Mok Sohn, Byoung-Kuk Na The Korean Journal of Parasitology.2018; 56(5): 409. CrossRef Cysteine protease involving in autophagosomal degradation of mitochondria during encystation of Acanthamoeba Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Hyun-Hee Kong Molecular and Biochemical Parasitology.2012; 185(2): 121. CrossRef Acanthamoeba culbertsoni Elicits Soluble Factors That Exert Anti-Microglial Cell Activity Jenica L. Harrison, Gabriela A. Ferreira, Erinn S. Raborn, Audrey D. Lafrenaye, Francine Marciano-Cabral, Guy A. Cabral Infection and Immunity.2010; 78(9): 4001. CrossRef Characterization of a Serine Proteinase Mediating Encystation of Acanthamoeba Eun-Kyung Moon, Dong-Il Chung, Yeon-Chul Hong, Hyun-Hee Kong Eukaryotic Cell.2008; 7(9): 1513. CrossRef Differentially expressed genes of Acanthamoeba castellanii during encystation Eun-Kyung Moon, Dong-Il Chung, Yeon-Chul Hong, Hyun-Hee Kong The Korean Journal of Parasitology.2007; 45(4): 283. CrossRef Acanthamoeba: biology and increasing importance in human health Naveed Ahmed Khan FEMS Microbiology Reviews.2006; 30(4): 564. CrossRef Intracellular Localization and Trafficking of Serine Proteinase AhSub and Cysteine Proteinase AhCP of Acanthamoeba healyi E.-K. Moon, S.-T. Lee, D.-I. Chung, H.-H. Kong Eukaryotic Cell.2006; 5(1): 125. CrossRef Pathogenic free-living amoebae in Korea Ho-Joon Shin, Kyung-il Im The Korean Journal of Parasitology.2004; 42(3): 93. CrossRef 8,579 View 96 Download Crossref Phylogenetic relationships among Acanthamoeba spp. based on PCR-RFLP analyses of mitochondrial small subunit rRNA gene Hak-Sun Yu, Mee-Yul Hwang, Tae-Ook Kim, Ho-Cheol Yun, Tae-Ho Kim, Hyun-Hee Kong, Dong-Il Chung Korean J Parasitol 1999;37(3):181-188. Published online September 30, 1999 DOI: https://doi.org/10.3347/kjp.1999.37.3.181 Abstract PDF We investigated the value of mitochondrial small subunit rRNA gene (mt SSU rDNA) PCR-RFLP as a taxonomic tool for Acanthamoeba isolates with close interrelationships. Twenty-five isolates representing 20 species were included in the analysis. As in nuclear 18S rDNA analysis, two type strains (A. astronyxis and A. tubiashi) of morphological group 1 diverged earliest from the other strains, but the divergence between them was less than in 18S riboprinting. Acanthamoeba griffini of morphological group 2 branched between pathogenic (A. culbertsoni A-1 and A. healyi OC-3A) and nonpathogenic (A. palestinensis Reich, A. pustulosa GE-3a, A. royreba Oak Ridge, and A lenticulata PD2S) strains of morphological group 3. Among the remaining isolates of morphological group 2, the Chang strain had the identical mitochondrial riboprints as the type strain of A. hatchetti. AA2 and AA1, the type strains of A. divionensis and A. paradivionensis, respectively, had the identical riboprints as A. quina Vil3 and A. castellanii Ma. Although the branching orders of A. castellanii Neff, A. polyphaga P23, A. triangularis SH621, and A. lugdunensis L3a were different from those in 18S riboprinting analysis, the results obtained from this study generally coincided well with those from 18S riboprinting. Mitochondrial riboprinting may have an advantage over nuclear 18S rDNA riboprinting because the mt SSU rDNAs do not seem to have introns that are found in the 18S genes of Acanthamoeba and that distort phylogenetic analyses. Citations Citations to this article as recorded by *Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba* Species an Indicator of Pathogenicity?** Merve Kahraman, Zübeyda Akın Polat Turkish Journal of Parasitology.2024; 48(1): 15. CrossRef The Status of Molecular Analyses of Isolates of Acanthamoeba Maintained by International Culture Collections Paul A. Fuerst Microorganisms.2023; 11(2): 295. CrossRef Acanthamoeba Mannose and Laminin Binding Proteins Variation across Species and Genotypes Daniele Corsaro Microorganisms.2022; 10(11): 2162. CrossRef DNA extraction from amoebal isolates and genotype determination of Acanthamoeba from tap water in Latvia Inese Gavarāne, Jūlija Trofimova, Artjoms Mališevs, Olga Valciņa, Muza Kirjušina, Ilze Rubeniņa, Aivars Bērziņš Parasitology Research.2018; 117(10): 3299. CrossRef Genotyping of Acanthamoeba T15: the environmental strain in Turkey G. Evyapan, I. S. Koltas, F. Eroglu Transactions of the Royal Society of Tropical Medicine and Hygiene.2015; 109(3): 221. CrossRef Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA Md Moshiur Rahman, Kenji Yagita, Akira Kobayashi, Yosaburo Oikawa, Amjad I.A. Hussein, Takahiro Matsumura, Masaharu Tokoro The Korean Journal of Parasitology.2013; 51(4): 401. CrossRef Molecular Phylogeny of Acanthamoeba Hyun Hee Kong The Korean Journal of Parasitology.2009; 47(Suppl): S21. CrossRef Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung The Korean Journal of Parasitology.2008; 46(3): 157. CrossRef Evaluation of taxonomic validity of four species of Acanthamoeba: A. divionensis, A. paradivionensis, A. mauritaniensis, and A. rhysodes, inferred from molecular analyses Hua Liu, Eun-Kyung Moon, Hak-Sun Yu, Hae-Jin Jeong, Yeon-Chul Hong, Hyun-Hee Kong, Dong-Il Chung The Korean Journal of Parasitology.2005; 43(1): 7. CrossRef Pathogenic free-living amoebae in Korea Ho-Joon Shin, Kyung-il Im The Korean Journal of Parasitology.2004; 42(3): 93. CrossRef A riboprinting scheme for identification of unknown Acanthamoeba isolates at species level Hyun-Hee Kong, Dong-Il Chung The Korean Journal of Parasitology.2002; 40(1): 25. CrossRef Use of Subgenic 18S Ribosomal DNA PCR and Sequencing for Genus and Genotype Identification of Acanthamoebae from Humans with Keratitis and from Sewage Sludge Jill M. Schroeder, Gregory C. Booton, John Hay, Ingrid A. Niszl, David V. Seal, Miles B. Markus, Paul A. Fuerst, Thomas J. Byers Journal of Clinical Microbiology.2001; 39(5): 1903. CrossRef Correlations between Morphological, Molecular Biological, and Physiological Characteristics in Clinical and Nonclinical Isolates of Acanthamoeba spp Julia Walochnik, Andreas Obwaller, Horst Aspöck Applied and Environmental Microbiology.2000; 66(10): 4408. CrossRef 9,702 View 77 Download Crossref