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Hui Jun Ren	1 Article
Molecular Identification of a Trichinella Isolate from a Naturally Infected Pig in Tibet, China Ling Zhao Li, Zhong Quan Wang, Peng Jiang, Xi Zhang, Hui Jun Ren, Jing Cui Korean J Parasitol 2011;49(4):381-384. Published online December 16, 2011 DOI: https://doi.org/10.3347/kjp.2011.49.4.381 Abstract PDF The first human case with trichinellosis was reported in 1964 in Tibet, China. However, up to the present, the etiological agent of trichinellosis has been unclear. The aim of this study was to identify a Tibet Trichinella isolate at a species level by PCR-based methods. Multiplex PCR revealed amplicon of the expected size (173 bp) for Trichinella spiralis in assays containing larval DNA from Tibet Trichinella isolate from a naturally infected pig. The Tibet Trichinella isolate was also identified by PCR amplification of the 5S ribosomal DNA intergenic spacer region (5S ISR) and mitochondrial large-subunit ribosomal RNA (mt-lsrDNA) gene sequences. The results showed that 2 DNA fragments (749 bp and 445 bp) of the Tibet Trichinella isolate were identical to that of the reference isolates of T. spiralis. The Tibet Trichinella isolate might be classifiable to T. spiralis. This is the first report on T. spiralis in southwestern China. Citations Citations to this article as recorded by Genetic identification of Trichinella species found in wild carnivores from the territory of Kazakhstan Rabiga Uakhit, Anne Mayer-Scholl, Chincher Shin, Ainura Smagulova, Lyudmila Lider, Sergey Leontyev, Vladimir Kiyan Frontiers in Veterinary Science.2023;[Epub] CrossRef Molecular characterization of a Trichinella spiralis elastase-1 and its potential as a diagnostic antigen for trichinellosis Chen Xi Hu, Peng Jiang, Xin Yue, Jie Zeng, Xin Zhuo Zhang, Yan Yan Song, Ruo Dan Liu, Xi Zhang, Zhong Quan Wang, Jing Cui Parasites & Vectors.2020;[Epub] CrossRef Surveillance and diagnosis of zoonotic foodborne parasites Reza Zolfaghari Emameh, Sami Purmonen, Antti Sukura, Seppo Parkkila Food Science & Nutrition.2018; 6(1): 3. CrossRef Innovative molecular diagnosis of Trichinella species based on β‐carbonic anhydrase genomic sequence Reza Zolfaghari Emameh, Marianne Kuuslahti, Anu Näreaho, Antti Sukura, Seppo Parkkila Microbial Biotechnology.2016; 9(2): 172. CrossRef Comparison of three molecular detection methods for detection of Trichinella in infected pigs Zhibing Lin, Jie Cao, Houshuang Zhang, Yongzhi Zhou, Mingjun Deng, Guoqing Li, Jinlin Zhou Parasitology Research.2013; 112(5): 2087. CrossRef 8,860 View 82 Download Crossref