Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these represent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.
Citations
Citations to this article as recorded by
The Distribution Characteristics of Microsatellites in the Transcriptome of Periplaneta americana Lifang Zhang, Shangeng He Cytology and Genetics.2023; 57(5): 491. CrossRef
Identification and characterization of microRNAs in American cockroach (Periplaneta americana) Qiao Yang, Zheng Bao, Mingyu Yang, Yongmei Shen, Xiuyue Zhang, Bisong Yue, Yang Meng, Zhenxin Fan Gene.2020; 743: 144610. CrossRef
New insight into foregut functions of xenobiotic detoxification in the cockroach Periplaneta americana Jian‐Hua Zhang, Song Zhang, Yuan‐Xue Yang, Yi‐Xi Zhang, Ze‐Wen Liu Insect Science.2018; 25(6): 978. CrossRef
Cockroach allergen serine proteinases: Isolation, sequencing and signalling via proteinase‐activated receptor‐2 D. J. Polley, K. Mihara, R. Ramachandran, H. Vliagoftis, B. Renaux, M. Saifeddine, M. O. Daines, S. Boitano, M. D. Hollenberg Clinical & Experimental Allergy.2017; 47(7): 946. CrossRef
De Novo Transcriptome Analysis and Detection of Antimicrobial Peptides of the American Cockroach Periplaneta americana (Linnaeus) In-Woo Kim, Joon Ha Lee, Sathiyamoorthy Subramaniyam, Eun-Young Yun, Iksoo Kim, Junhyung Park, Jae Sam Hwang, Surajit Bhattacharjya PLOS ONE.2016; 11(5): e0155304. CrossRef
Midgut Transcriptome of the Cockroach Periplaneta americana and Its Microbiota: Digestion, Detoxification and Oxidative Stress Response Jianhua Zhang, Yixi Zhang, Jingjing Li, Meiling Liu, Zewen Liu, Xinghui Qiu PLOS ONE.2016; 11(5): e0155254. CrossRef
Analysis of German cockroach (Blattella germanica) expressed sequence tags Ji Eun Jeong, Hee Ju Hwang, Hong Seog Park, Hee Jae Cha, Yong Seok Lee, Meesun Ock Genes & Genomics.2015; 37(3): 271. CrossRef
Allergenic Characterization of a Novel Allergen, Homologous to Chymotrypsin, from German Cockroach Kyoung Yong Jeong, Mina Son, Jae-Hyun Lee, Chein-Soo Hong, Jung-Won Park Allergy, Asthma & Immunology Research.2015; 7(3): 283. CrossRef
De novo Assembly and Characterization of the Testis Transcriptome and Development of EST-SSR Markers in the Cockroach Periplaneta americana Wan Chen, Yu-Xiang Liu, Guo-Fang Jiang Scientific Reports.2015;[Epub] CrossRef
De Novo Transcriptome of the Hemimetabolous German Cockroach (Blattella germanica) Xiaojie Zhou, Kun Qian, Ying Tong, Junwei Jerry Zhu, Xinghui Qiu, Xiaopeng Zeng, Yara M. Traub-Csekö PLoS ONE.2014; 9(9): e106932. CrossRef
Identification of differentially expressed genes in American cockroach ovaries and testes by suppression subtractive hybridization and the prediction of its miRNAs Wan Chen, Guo-Fang Jiang, Shu-Hong Sun, Yong Lu, Fei Ma, Bin Li Molecular Genetics and Genomics.2013; 288(11): 627. CrossRef
