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Hyo-Kyung Kim	3 Articles
Purification and characterization of a 33 kDa serine protease from Acanthamoeba lugdunensis KA/E2 isolated from a Korean keratitis patient Hyo-Kyung Kim, Young-Ran Ha, Hak-Sun Yu, Hyun-Hee Kong, Dong-Il Chung Korean J Parasitol 2003;41(4):189-196. Published online December 20, 2003 DOI: https://doi.org/10.3347/kjp.2003.41.4.189 Abstract PDF In order to evaluate the possible roles of secretory proteases in the pathogenesis of amoebic keratitis, we purified and characterized a serine protease secreted by Acanthamoeba lugdunensis KA/E2, isolated from a Korean keratitis patient. The ammonium sulfate-precipitated culture supernatant of the isolate was purified by sequential chromatography on CM-Sepharose, Sephacryl S-200, and mono Q-anion exchange column. The purified 33 kDa protease had a pH optimum of 8.5 and a temperature optimum of 55℃. Phenylmethylsulfonylfluoride and 4-(2-Aminoethyl)-benzenesulfonyl-fluoride, both serine protease specific inhibitors, inhibited almost completely the activity of the 33 kDa protease whereas other classes of inhibitors did not affect its activity. The 33 kDa enzyme degraded various extracellular matrix proteins and serum proteins. Our results strongly suggest that the 33 kDa serine protease secreted from this keratopathogenic Acanthamoeba play important roles in the pathogenesis of amoebic keratitis, such as in corneal tissue invasion, immune evasion and nutrient uptake. Citations Citations to this article as recorded by Identification of an Antimicrobial Protease from Acanthamoeba via a Novel Zymogram Alvaro de Obeso Fernández del Valle, Luis Javier Melgoza-Ramírez, María Fernanda Esqueda Hernández, Alfonso David Rios-Pérez, Sutherland K. Maciver Processes.2023; 11(9): 2620. CrossRef The gene expression and proteomic profiling of Acanthamoeba isolates Chayan Sharma, Sumeeta Khurana, Alka Bhatia, Amit Arora, Amit Gupta Experimental Parasitology.2023; 255: 108630. CrossRef Comparative Surfaceome Analysis of Clonal Histomonas meleagridis Strains with Different Pathogenicity Reveals Strain-Dependent Profiles Marcelo de Jesus Ramires, Karin Hummel, Tamas Hatfaludi, Petra Riedl, Michael Hess, Ivana Bilic Microorganisms.2022; 10(10): 1884. CrossRef Host Invasion by Pathogenic Amoebae: Epithelial Disruption by Parasite Proteins Abigail Betanzos, Cecilia Bañuelos, Esther Orozco Genes.2019; 10(8): 618. CrossRef Acanthamoeba spp. un agente oportunista en infecciones humanas Martín Cabello-Vílchez Revista de Investigación de la Universidad Privada Norbert Wiener.2019; 4(1): 11. CrossRef Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells Showgy Y. Ma’ayeh, Jingyi Liu, Dimitra Peirasmaki, Katarina Hörnaeus, Sara Bergström Lind, Manfred Grabherr, Jonas Bergquist, Staffan G. Svärd, Armando Jardim PLOS Neglected Tropical Diseases.2017; 11(12): e0006120. CrossRef Purification and Characterization of Extracellular Protease and Amylase Produced by the Bacterial Strain, Corynebacterium alkanolyticum ATH3 Isolated from Fish Gut Goutam Banerjee, Sandip Mukherjee, Shelley Bhattacharya, Arun K. Ray Arabian Journal for Science and Engineering.2016; 41(1): 9. CrossRef Microbial collagenases: challenges and prospects in production and potential applications in food and nutrition Gaurav Kumar Pal, Suresh PV RSC Advances.2016; 6(40): 33763. CrossRef Diversity, Structures, and Collagen-Degrading Mechanisms of Bacterial Collagenolytic Proteases Yu-Zhong Zhang, Li-Yuan Ran, Chun-Yang Li, Xiu-Lan Chen, F. E. Löffler Applied and Environmental Microbiology.2015; 81(18): 6098. CrossRef Microarray and KOG analysis of Acanthamoeba healyi genes up-regulated by mouse-brain passage Eun-Kyung Moon, Ying-Hua Xuan, Hyun-Hee Kong Experimental Parasitology.2014; 143: 69. CrossRef Characterization of a Novel Subtilisin-like Protease Myroicolsin from Deep Sea Bacterium Myroides profundi D25 and Molecular Insight into Its Collagenolytic Mechanism Li-Yuan Ran, Hai-Nan Su, Ming-Yang Zhou, Lei Wang, Xiu-Lan Chen, Bin-Bin Xie, Xiao-Yan Song, Mei Shi, Qi-Long Qin, Xiuhua Pang, Bai-Cheng Zhou, Yu-Zhong Zhang, Xi-Ying Zhang Journal of Biological Chemistry.2014; 289(9): 6041. CrossRef Structural and mechanistic insights into collagen degradation by a bacterial collagenolytic serine protease in the subtilisin family Li‐Yuan Ran, Hai‐Nan Su, Guo‐Yan Zhao, Xiang Gao, Ming‐Yang Zhou, Peng Wang, Hui‐Lin Zhao, Bin‐Bin Xie, Xi‐Ying Zhang, Xiu‐Lan Chen, Bai‐Cheng Zhou, Yu‐Zhong Zhang Molecular Microbiology.2013; 90(5): 997. CrossRef Proteases fromEntamoebaspp. and Pathogenic Free-Living Amoebae as Virulence Factors Jesús Serrano-Luna, Carolina Piña-Vázquez, Magda Reyes-López, Guillermo Ortiz-Estrada, Mireya de la Garza Journal of Tropical Medicine.2013; 2013: 1. CrossRef Host-Parasite Interaction: Parasite-Derived and -Induced Proteases That Degrade Human Extracellular Matrix Carolina Piña-Vázquez, Magda Reyes-López, Guillermo Ortíz-Estrada, Mireya de la Garza, Jesús Serrano-Luna Journal of Parasitology Research.2012; 2012: 1. CrossRef Distribution of Proteolytic Activity in the Different Protein Fractions of Tropical Shrimp Head Waste Raghu Ganugula, Rupsankar Chakrabarti, Krothapalli Raja Surya Sambasiva Rao Food Biotechnology.2008; 22(1): 18. CrossRef Protease activity in extracellular products secreted in vitro by trophozoites of Giardia duodenalis Thaís Batista de Carvalho, Érica Boarato David, Silvana Torossian Coradi, Semíramis Guimarães Parasitology Research.2008; 104(1): 185. CrossRef Comparison of specific activity and cytopathic effects of purified 33 kDa serine proteinase from Acanthamoeba strains with different degree of virulence Won-Tae Kim, Hyun-Hee Kong, Young-Ran Ha, Yeon-Chul Hong, Hae Jin Jeong, Hak Sun Yu, Dong-Il Chung The Korean Journal of Parasitology.2006; 44(4): 321. CrossRef Detection of a serine proteinase gene in Acanthamoeba genotype T6 (Amoebozoa: Lobosea) Marion Blaschitz, Martina Köhsler, Horst Aspöck, Julia Walochnik Experimental Parasitology.2006; 114(1): 26. CrossRef Intracellular Localization and Trafficking of Serine Proteinase AhSub and Cysteine Proteinase AhCP of Acanthamoeba healyi E.-K. Moon, S.-T. Lee, D.-I. Chung, H.-H. Kong Eukaryotic Cell.2006; 5(1): 125. CrossRef Étude de l’effet des oligomères procyanidoliques sur la fibrillogénèse de la cornée A.M. Robert, L. Robert, G. Renard Journal Français d'Ophtalmologie.2005; 28(10): 1017. CrossRef Expression of the nfa1 Gene Cloned from Pathogenic Naegleria fowleri in Nonpathogenic N. gruberi Enhances Cytotoxicity against CHO Target Cells In Vitro Seok-Ryoul Jeong, Sang-Chul Lee, Kyoung-Ju Song, Sun Park, Kyongmin Kim, Myung-Hee Kwon, Kyung-il Im, Ho-Joon Shin Infection and Immunity.2005; 73(7): 4098. CrossRef Extracellular proteases of Acanthamoeba castellanii (encephalitis isolate belonging to T1 genotype) contribute to increased permeability in an in vitro model of the human blood–brain barrier Selwa Alsam, James Sissons, Samantha Jayasekera, Naveed Ahmed Khan Journal of Infection.2005; 51(2): 150. CrossRef 8,666 View 87 Download Crossref Genetic analyses of Acanthamoeba isolates from contact lens storage cases of students in Seoul, Korea Hak-Sun Yu, Kyung-Hee Choi, Hyo-Kyung Kim, Hyun-Hee Kong, Dong-Il Chung Korean J Parasitol 2001;39(2):161-170. Published online June 30, 2001 DOI: https://doi.org/10.3347/kjp.2001.39.2.161 Abstract PDF We conducted both the small subunit ribosomal DNA (SSU rDNA) polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and mitochondrial (mt) DNA RFLP analyses for a genetic characterization of Acanthamoeba isolates from contact lens storage cases of students in Seoul, Korea. Twenty-three strains of Acanthamoeba from the American Type Culture Collection and twelve clinical isolates from Korean patients were used as reference strains. Thirty-nine isolates from contact lens storage cases were classified into seven types (KA/LS1, KA/LS2, KA/LS4, KA/LS5, KA/LS7, KA/LS18, KA/LS31). Four types (KA/LS1, KA/LS2, KA/LS5, KA/LS18) including 33 isolates were regarded as A. castellanii complex by riboprints. KA/LS1 type was the most predominant (51.3%) in the present survey area, followed by KA/LS2 (20.9%), and KA/LS5 (7.7%) types. Amoebae of KA/LS1 type had the same mtDNA RFLP and riboprint patterns as KA/E2 and KA/E12 strains, clinical isolates from Korean keratitis patients. Amoebae of KA/LS2 type had the identical mtDNA RFLP patterns with A. castellanii Ma strain, a corneal isolate from an American patient as amoebae of KA/LS5 type, with KA/E3 and KA/E8 strains from other Korean keratitis patients. Amoebae of KA/LS18 type had identical patterns with JAC/E1, an ocular isolate from a Japanese patient. Three types, which remain unidentified at species level, were not corresponded with any clinical isolate in their mtDNA RFLP and riboprint patterns. Out of 39 isolates analyzed in this study, mtDNA RFLP and riboprint patterns of 33 isolates (84.6%) were identical to already known clinical isolates, and therefore, they may be regarded as potentially keratopathogenic. These results suggest that contact lens wearers in Seoul should pay more attention to hygienic maintenance of contact lens storage cases for the prevention of Acanthamoeba keratitis. Citations Citations to this article as recorded by Parasitological and microbiological assessment of contact lens storage cases: a survey of asymptomatic lens student wearers from five medical specialties in Tunisia, North Africa Sameh Belgacem, Raja Chaâbane-Banaoues, Amira Mejri, Sawsen Ben Ifa, Maha Mastouri, Hamouda Babba BMC Infectious Diseases.2025;[Epub] CrossRef The amoebicidal effect of Torreya nucifera extract on Acanthamoeba lugdunensis Min Seung Kang, Sangyoon Kim, Da Som Kim, Hak Sun Yu, Ji Eun Lee, Yi Cao PLOS ONE.2023; 18(2): e0281141. CrossRef The First Case of Cutaneous Acanthamoebiasis Caused by Acanthamoeba triangularis in Korea Mi Soo Choi, Na Hye Myong, Min Seo, Sukbin Jang, Dae Kwan Yun, Kyujin Yeom, Dong-Il Chung, Byung Cheol Park, Yeonchul Hong, Myung Hwa Kim Annals of Dermatology.2023; 35(Suppl 2): S275. CrossRef Adhesion ofAcanthamoebaon Cosmetic Contact Lenses Seung-Mok Lee, Ji-Eun Lee, Da-In Lee, Hak-Sun Yu Journal of Korean Medical Science.2018;[Epub] CrossRef Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach Angela Magnet, Thiago Santos Gomes, Carmen Pardinas, Natalia Garcia de Blas, Cruz Sadaba, Eugenia Carrillo, Fernando Izquierdo, José Manuel Benítez del Castillo, Carolina Hurtado, Carmen del Aguila, Soledad Fenoy Parasites & Vectors.2018;[Epub] CrossRef Effect of Multipurpose Solution Combined With Autophagy Inhibitors on Adhesion of Acanthamoeba trophozoites to Silicone Hydrogel Contact Lenses Seung-Mok Lee, Da-In Lee, Sung-Hee Park, Hak-Sun Yu, Ji-Eun Lee, Jong-Soo Lee Cornea.2017; 36(12): 1538. CrossRef Effects of multipurpose solutions on the adhesion of Acanthamoeba to rigid gas permeable contact lenses Ga‐Hyun Lee, Hak‐Sun Yu, Ji‐Eun Lee Ophthalmic and Physiological Optics.2016; 36(2): 93. CrossRef Adhesion of Acanthamoeba on Silicone Hydrogel Contact Lenses Ga-Hyun Lee, Ji-Eun Lee, Mi-Kyung Park, Hak-Sun Yu Cornea.2016; 35(5): 663. CrossRef Acanthamoeba spp. in Contact Lenses from Healthy Individuals from Madrid, Spain Thiago dos Santos Gomes, Angela Magnet, Fernando Izquierdo, Lucianna Vaccaro, Fernando Redondo, Sara Bueno, Maria Luisa Sánchez, Santiago Angulo, Soledad Fenoy, Carolina Hurtado, Carmen del Aguila, Graham R. Wallace PLOS ONE.2016; 11(4): e0154246. CrossRef 18S ribosomal DNA genotypes of Acanthamoeba species isolated from contact lens cases in the Philippines Windell L. Rivera, Davin Edric V. Adao Parasitology Research.2009; 105(4): 1119. CrossRef Endosymbionts of Acanthamoeba Isolated from Domestic Tap Water in Korea Seon Hee Choi, Min Kyoung Cho, Soon Cheol Ahn, Ji Eun Lee, Jong Soo Lee, Dong-Hee Kim, Ying-Hua Xuan, Yeon Chul Hong, Hyun Hee Kong, Dong Il Chung, Hak Sun Yu The Korean Journal of Parasitology.2009; 47(4): 337. CrossRef Molecular Phylogeny of Acanthamoeba Hyun Hee Kong The Korean Journal of Parasitology.2009; 47(Suppl): S21. CrossRef Detection of Acanthamoeba in Tap Water and Contact Lens Cases Using Polymerase Chain Reaction Maureen Boost, Pauline Cho, Sindy Lai, Wing-Man Sun Optometry and Vision Science.2008; 85(7): 526. CrossRef Acanthamoeba spp. and bacterial contamination in contact lens storage cases and the relationship to user profiles Claiton José Pens, Marisa da Costa, Cristina Fadanelli, Karin Caumo, MariliseBrittes Rott Parasitology Research.2008; 103(6): 1241. CrossRef Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung The Korean Journal of Parasitology.2008; 46(3): 157. CrossRef Distribution of Acanthamoeba spp. in Raw Water and Water Treatment Process Journal of the Environmental Sciences.2008; 17(10): 1121. CrossRef Molecular characterization of bacterial endosymbionts of Acanthamoeba isolates from infected corneas of Korean patients Ying-Hua Xuan, Hak Sun Yu, Hae Jin Jeong, Sung-Yong Seol, Dong-Il Chung, Hyun-Hee Kong The Korean Journal of Parasitology.2007; 45(1): 1. CrossRef Cysticidal Effect on Acanthamoeba and Toxicity on Human Keratocytes by Polyhexamethylene Biguanide and Chlorhexidine Ji-Eun Lee, Boo Sup Oum, Hee Young Choi, Hak Sun Yu, Jong Soo Lee Cornea.2007; 26(6): 736. CrossRef Acanthamoeba: Keratopathogenicity of isolates from domestic tap water in Korea Hae Jin Jeong, Sun Joo Lee, Jeong Hwan Kim, Ying Hua Xuan, Keun Hee Lee, Sang Kyun Park, Sun Hee Choi, Dong Il Chung, Hyun Hee Kong, Mee Sun Ock, Hak Sun Yu Experimental Parasitology.2007; 117(4): 357. CrossRef Factors Affecting the Epidemiology ofAcanthamoebaKeratitis Youhanna W. Ibrahim, David L. Boase, Ian A. Cree Ophthalmic Epidemiology.2007; 14(2): 53. CrossRef Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear Sun Joo Lee, Hae Jin Jeong, Ji Eun Lee, Jong Soo Lee, Ying Hua Xuan, Hyun-Hee Kong, Dong-Il Chung, Mee-Sun Ock, Hak Sun Yu The Korean Journal of Parasitology.2006; 44(4): 313. CrossRef The role of domestic tap water in Acanthamoeba contamination in contact lens storage cases in Korea Hae Jin Jeong, Hak Sun Yu The Korean Journal of Parasitology.2005; 43(2): 47. CrossRef Acanthamoeba keratitis update—incidence, molecular epidemiology and new drugs for treatment D V Seal Eye.2003; 17(8): 893. CrossRef Mitochondrial DNA Restriction Fragment Length Polymorphism (RFLP) and 18S Small-Subunit Ribosomal DNA PCR-RFLP Analyses ofAcanthamoebaIsolated from Contact Lens Storage Cases of Residents in Southwestern Korea Hyun-Hee Kong, Ji-Yeol Shin, Hak-Sun Yu, Jin Kim, Tae-Won Hahn, Young-Ho Hahn, Dong-Il Chung Journal of Clinical Microbiology.2002; 40(4): 1199. CrossRef 9,414 View 53 Download Crossref Expressed sequence tags (ESTs) analysis of Acanthamoeba healyi Hyun-Hee Kong, Mee-Yeul Hwang, Hyo-Kyung Kim, Dong-Il Chung Korean J Parasitol 2001;39(2):151-160. Published online June 30, 2001 DOI: https://doi.org/10.3347/kjp.2001.39.2.151 Abstract PDF Randomly selected 435 clones from Acanthamoeba healyi cDNA library were sequenced and a total of 387 expressed sequence tags (ESTs) had been generated. Based on the results of BLAST search, 130 clones (34.4%) were identified as the genes enconding surface proteins, enzymes for DNA, energy production or other metabolism, kinases and phosphatases, protease, proteins for signal transduction, structural and cytoskeletal proteins, cell cycle related proteins, transcription factors, transcription and translational machineries, and transporter proteins. Most of the genes (88.5%) are newly identified in the genus Acanthamoeba. Although 15 clones matched the genes of Acanthamoeba located in the public databases, twelve clones were actin gene which was the most frequently expressed gene in this study. These ESTs of Acanthamoeba would give valuable information to study the organism as a model system for biological investigations such as cytoskeleton or cell movement, signal transduction, transcriptional and translational regulations. These results would also provide clues to elucidate factors for pathogenesis in human granulomatous amoebic encephalitis or keratitis by Acanthamoeba. Citations Citations to this article as recorded by Construction of EST Database for Comparative Gene Studies of Acanthamoeba Eun-Kyung Moon, Joung-Ok Kim, Ying-Hua Xuan, Young-Sun Yun, Se Won Kang, Yong Seok Lee, Tae-In Ahn, Yeon-Chul Hong, Dong-Il Chung, Hyun-Hee Kong The Korean Journal of Parasitology.2009; 47(2): 103. CrossRef Acanthamoeba Interaction with Extracellular Matrix Glycoproteins: Biological and Biochemical Characterization and Role in Cytotoxicity and Invasiveness BRUNO DA ROCHA‐AZEVEDO, MELISSA JAMERSON, GUY A. CABRAL, FERNANDO C. SILVA‐FILHO, FRANCINE MARCIANO‐CABRAL Journal of Eukaryotic Microbiology.2009; 56(3): 270. CrossRef Sampling Gene Diversity Across the Supergroup Amoebozoa: Large EST Data Sets from Acanthamoeba castellanii, Hartmannella vermiformis, Physarum polycephalum, Hyperamoeba dachnaya and Hyperamoeba sp. Russell F. Watkins, Michael W. Gray Protist.2008; 159(2): 269. CrossRef Anisakis simplex: Analysis of expressed sequence tags (ESTs) of third-stage larva Hak Sun Yu, Sang Kyun Park, Keun Hee Lee, Sun Joo Lee, Sun Hee Choi, Mee Sun Ock, Hae Jin Jeong Experimental Parasitology.2007; 117(1): 51. CrossRef Molecular and biochemical characterization of a novel actin bundling protein in Acanthamoeba Joanna It-itan Alafag, Eun-Kyung Moon, Yeon-Chul Hong, Dong-Il Chung, Hyun-Hee Kong The Korean Journal of Parasitology.2006; 44(4): 331. CrossRef Acanthamoeba healyi: Molecular cloning and characterization of a coronin homologue, an actin-related protein Eleonor T. Baldo, Eun-Kyung Moon, Hyun-Hee Kong, Dong-Il Chung Experimental Parasitology.2005; 110(2): 114. CrossRef 7,960 View 55 Download Crossref