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Ji Eun Lee	2 Articles
Endosymbionts of Acanthamoeba Isolated from Domestic Tap Water in Korea Seon Hee Choi, Min Kyoung Cho, Soon Cheol Ahn, Ji Eun Lee, Jong Soo Lee, Dong-Hee Kim, Ying-Hua Xuan, Yeon Chul Hong, Hyun Hee Kong, Dong Il Chung, Hak Sun Yu Korean J Parasitol 2009;47(4):337-344. Published online December 1, 2009 DOI: https://doi.org/10.3347/kjp.2009.47.4.337 Abstract PDF In a previous study, we reported our discovery of Acanthamoeba contamination in domestic tap water; in that study, we determined that some Acanthamoeba strains harbor endosymbiotic bacteria, via our molecular characterization by mitochondrial DNA restriction fragment length polymorphism (Mt DNA RFLP). Five (29.4%) among 17 Acanthamoeba isolates contained endosymbionts in their cytoplasm, as demonstrated via orcein staining. In order to estimate their pathogenicity, we conducted a genetic characterization of the endosymbionts in Acanthamoeba isolated from domestic tap water via 16S rDNA sequencing. The endosymbionts of Acanthamoeba sp. KA/WP3 and KA/WP4 evidenced the highest level of similarity, at 97% of the recently published 16S rDNA sequence of the bacterium, Candidatus Amoebophilus asiaticus. The endosymbionts of Acanthamoeba sp. KA/WP8 and KA/WP12 shared a 97% sequence similarity with each other, and were also highly similar to Candidatus Odyssella thessalonicensis, a member of the α-proteobacteria. The endosymbiont of Acanthamoeba sp. KA/WP9 exhibits a high degree of similarity (85-95%) with genus Methylophilus, which is not yet known to harbor any endosymbionts. This is the first report, to the best of our knowledge, to show that Methylophilus spp. can live in the cytoplasm of Acanthamoeba. Citations Citations to this article as recorded by An Evolutionary-Focused Review of the Holosporales (Alphaproteobacteria): Diversity, Host Interactions, and Taxonomic Re-ranking as Holosporineae Subord. Nov Michele Castelli, Giulio Petroni Microbial Ecology.2025;[Epub] CrossRef Zooming in on the intracellular microbiome composition of bacterivorous Acanthamoeba isolates Binod Rayamajhee, Mark Willcox, Savitri Sharma, Ronnie Mooney, Constantinos Petsoglou, Paul R Badenoch, Samendra Sherchan, Fiona L Henriquez, Nicole Carnt ISME Communications.2024;[Epub] CrossRef Acanthamoeba species isolated from marine water in Malaysia exhibit distinct genotypes and variable physiological properties Rosnani Hanim Mohd Hussain, Mohamed Kamel Abdul Ghani, Naveed Ahmed Khan, Ruqaiyyah Siddiqui, Tengku Shahrul Anuar Journal of Water and Health.2022; 20(1): 54. CrossRef The life cycle-dependent transcriptional profile of the obligate intracellular amoeba symbiontAmoebophilus asiaticus E Selberherr, T Penz, L König, B Conrady, A Siegl, M Horn, S Schmitz-Esser FEMS Microbiology Ecology.2022;[Epub] CrossRef A Systematic Review of Intracellular Microorganisms within Acanthamoeba to Understand Potential Impact for Infection Binod Rayamajhee, Dinesh Subedi, Hari Kumar Peguda, Mark Duncan Willcox, Fiona L. Henriquez, Nicole Carnt Pathogens.2021; 10(2): 225. CrossRef A comparative analysis of drinking water employing metagenomics Kyle D. Brumfield, Nur A. Hasan, Menu B. Leddy, Joseph A. Cotruvo, Shah M. Rashed, Rita R. Colwell, Anwar Huq, Christopher Staley PLOS ONE.2020; 15(4): e0231210. CrossRef Detection of bacterial endosymbionts in freshwater crustaceans: the applicability of non-degenerate primers to amplify the bacterial 16S rRNA gene Monika Mioduchowska, Michał Jan Czyż, Bartłomiej Gołdyn, Adrianna Kilikowska, Tadeusz Namiotko, Tom Pinceel, Małgorzata Łaciak, Jerzy Sell PeerJ.2018; 6: e6039. CrossRef Interactive effects of temperature, organic carbon, and pipe material on microbiota composition and Legionella pneumophila in hot water plumbing systems Caitlin R. Proctor, Dongjuan Dai, Marc A. Edwards, Amy Pruden Microbiome.2017;[Epub] CrossRef Isolation and Genotyping of Acanthamoeba spp. as Neglected Parasites in North of Iran Azar Shokri, Shahabeddin Sarvi, Ahmad Daryani, Mehdi Sharif The Korean Journal of Parasitology.2016; 54(4): 447. CrossRef Identification of Paenibacillus as a Symbiont in Acanthamoeba Vinicius José Maschio, Gertrudes Corção, Francielle Bücker, Karin Caumo, Marilise Brittes Rott Current Microbiology.2015; 71(3): 415. CrossRef Ankyrin‐repeat proteins from sponge symbionts modulate amoebal phagocytosis Mary T. H. D. Nguyen, Michael Liu, Torsten Thomas Molecular Ecology.2014; 23(6): 1635. CrossRef Infectious ophthalmology, a neglected field in clinical microbiology M. Drancourt Clinical Microbiology and Infection.2013; 19(3): 209. CrossRef Isolation and molecular characterization of potentially pathogenic Acanthamoeba genotypes from diverse water resources including household drinking water from Khyber Pakhtunkhwa, Pakistan Tania Tanveer, Abdul Hameed, Ambreen Gul Muazzam, Suk-Yul Jung, Asma Gul, Abdul Matin Parasitology Research.2013; 112(8): 2925. CrossRef Vahlkampfia sp: Structural observations of cultured trophozoites Arturo González-Robles, Lizbeth Salazar-Villatoro, Mónica González-Lázaro, Maritza Omaña-Molina, Adolfo Martínez-Palomo Experimental Parasitology.2012; 130(1): 86. CrossRef Diversity of bacteria and mycobacteria in biofilms of two urban drinking water distribution systems Ruyin Liu, Zhisheng Yu, Hongxun Zhang, Min Yang, Baoyou Shi, Xinchun Liu Canadian Journal of Microbiology.2012; 58(3): 261. CrossRef A bacterial genome in transition - an exceptional enrichment of IS elements but lack of evidence for recent transposition in the symbiont Amoebophilus asiaticus Stephan Schmitz-Esser, Thomas Penz, Anja Spang, Matthias Horn BMC Evolutionary Biology.2011;[Epub] CrossRef 12,046 View 123 Download Crossref Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear Sun Joo Lee, Hae Jin Jeong, Ji Eun Lee, Jong Soo Lee, Ying Hua Xuan, Hyun-Hee Kong, Dong-Il Chung, Mee-Sun Ock, Hak Sun Yu Korean J Parasitol 2006;44(4):313-320. Published online December 20, 2006 DOI: https://doi.org/10.3347/kjp.2006.44.4.313 Abstract PDF In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted mitochondrial DNA restriction fragment length polymorphism, 18S rDNA sequencing, and drug sensitivity analyses on the isolate (KA/PE1). The patient was treated with polyhexamethylene biguanide, chlorhexidine and oral itraconazole, which resulted in resolution of the patient's ocular inflammation. The majority of the molecular characteristics of the KA/PE1 were determined to be identical, or quite similar, to those of A. castellanii Ma strain, which had been isolated also from amoebic keratitis. The risk of Acanthamoeba keratitis as a potential complication of overnight orthokeratology is briefly discussed. Citations Citations to this article as recorded by Free-Living Amoeba Vermamoeba vermiformis Induces Allergic Airway Inflammation Da-In Lee, Sung Hee Park, Shin-Ae Kang, Do Hyun Kim, Sun Hyun Kim, So Yeon Song, Sang Eun Lee, Hak Sun Yu The Korean Journal of Parasitology.2022; 60(4): 229. CrossRef Orthokeratology lens-related Acanthamoeba keratitis: case report and analytical review Jinfang Wu, Huatao Xie Journal of International Medical Research.2021;[Epub] CrossRef Infectious keratitis and orthokeratology lens use: a systematic review Ka Wai Kam, Wing Yung, Gabriel Ka Hin Li, Li Jia Chen, Alvin L. Young Infection.2017; 45(6): 727. CrossRef The Role of Ultraviolet Radiation in the Ocular System of Mammals Mercede Majdi, Behrad Milani, Asadolah Movahedan, Lisa Wasielewski, Ali Djalilian Photonics.2014; 1(4): 347. CrossRef Orthokeratology lens related infections Kelvin Ho-Nam Wan World Journal of Ophthalmology.2014; 4(3): 63. CrossRef Riboflavin and Ultraviolet Light A Therapy as an Adjuvant Treatment for Medically Refractive Acanthamoeba Keratitis Yasin A. Khan, Renata T. Kashiwabuchi, Suy Anne Martins, Juan M. Castro-Combs, Sachin Kalyani, Philip Stanley, David Flikier, Ashley Behrens Ophthalmology.2011; 118(2): 324. CrossRef Bilateral Acanthamoeba Keratitis After Orthokeratology Eun Chul Kim, Man Soo Kim Cornea.2010; 29(6): 680. CrossRef Twenty Years of Acanthamoeba Keratitis F R S Carvalho, A S Foronda, M J Mannis, A L Höfling-Lima, R Belfort, Denise de Freitas Cornea.2009; 28(5): 516. CrossRef Molecular Phylogeny of Acanthamoeba Hyun Hee Kong The Korean Journal of Parasitology.2009; 47(Suppl): S21. CrossRef Bilateral Acanthamoeba Keratitis After Orthokeratology Eun Chul Kim, Man Soo Kim Cornea.2009; 28(3): 348. CrossRef Trends in Microbial Keratitis Associated With Orthokeratology Kathleen G. Watt, Helen A. Swarbrick Eye & Contact Lens: Science & Clinical Practice.2007; 33(6): 373. CrossRef 14,457 View 110 Download Crossref