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Joung-Ho Moon	2 Articles
Resistance to Toxoplasma gondii Infection in Mice Treated with Silk Protein by Enhanced Immune Responses Joung-Ho Moon, Kyoung-Ho Pyo, Bong-Kwang Jung, Hyang Sook Chun, Jong-Yil Chai, Eun-Hee Shin Korean J Parasitol 2011;49(3):303-308. Published online September 30, 2011 DOI: https://doi.org/10.3347/kjp.2011.49.3.303 Abstract PDF This study investigated whether elevated host immune capacity can inhibit T. gondii infection. For this purpose, we used silk protein extracted from Bombyx mori cocoons as a natural supplement to augment immune capacity. After silk protein administration to BALB/c mice for 6 weeks, ratios of T lymphocytes (CD4⁺ and CD8⁺ T-cells) and splenocyte proliferative capacities in response to Con A or T. gondii lysate antigen (TLA) were increased. Of various cytokines, which regulate immune systems, Th1 cytokines, such as IFN-γ, IL-2, and IL-12, were obviously increased in splenocyte primary cell cultures. Furthermore, the survival of T. gondii (RH strain)-infected mice increased from 2 days to 5 or more days. In a state of immunosuppression induced by methylprednisolone acetate, silk protein-administered mice were resistant to reduction in T-lymphocyte (CD4⁺ and CD8⁺ T-cells) numbers and the splenocyte proliferative capacity induced by Con A or TLA with a statistical significance. Taken together, our results suggest that silk protein augments immune capacity in mice and the increased cellular immunity by silk protein administration increases host protection against acute T. gondii infection. Citations Citations to this article as recorded by Oral Toxicity and Hypotensive Influence of Sericin-Derived Oligopeptides (SDOs) from Yellow Silk Cocoons of Bombyx mori in Rodent Studies Chainarong Tocharus, Virakboth Prum, Manote Sutheerawattananonda Foods.2024; 13(21): 3505. CrossRef Low dose administration of mature silkworm powder induces gastric mucosal defense factors in ethanol-induced gastric injury rat model Da-Young Lee, Moon-Young Song, Kyung-Sook Hong, Sun-Mi Yun, Young-Min Han, Eun-Hee Kim Food Science and Biotechnology.2023; 32(11): 1551. CrossRef Dietary administration of silk microparticles improves the epidermal and dermal regeneration after a skin wounding in gilthead seabream (Sparus aurata L.) Nora Albaladejo-Riad, Cristóbal Espinosa Ruiz, M. Ángeles Esteban Fish & Shellfish Immunology.2022; 124: 92. CrossRef The effect of silk peptide on immune system, A randomized, double-blind, placebo-controlled clinical trial Jin-Taek Hwang, Jung Min Cho, In Hye Jeong, Jeong-yong Lee, Ki-Chan Ha, Hyang-Im Baek, Hye Jeong Yang, Min Jung Kim, Jong Ho Lee Journal of Functional Foods.2019; 55: 275. CrossRef Silk peptide treatment potentiates natural killer cell activity in vitro and induces natural killer cell maturation and activation in mouse splenocytes Sun-Hee Jang, Mi-Sun Oh, Hyang-Im Baek, Ki-Chan Ha, Jeong-Yong Lee, Yong-Suk Jang Pharmaceutical Biology.2019; 57(1): 369. CrossRef Oral Administration of Silk Peptide Enhances the Maturation and Cytolytic Activity of Natural Killer Cells Sun-Hee Jang, Mi-Sun Oh, Hyang-Im Baek, Ki-Chan Ha, Jeong-Yong Lee, Yong-Suk Jang Immune Network.2018;[Epub] CrossRef Biomaterials-Based Vaccination Strategies for the Induction of CD8+T Cell Responses Charles B. Chesson, Shaunte Ekpo-Otu, Janice J. Endsley, Jai S. Rudra ACS Biomaterials Science & Engineering.2017; 3(2): 126. CrossRef STAT6 Expression and IL-13 Production in Association with Goblet Cell Hyperplasia and Worm Expulsion of Gymnophalloides seoi from C57BL/6 Mice Jin-Joo Lee, Donghee Kim, Kyoung-Ho Pyo, Min-Ki Kim, Hyo-Jin Kim, Jong-Yil Chai, Eun-Hee Shin The Korean Journal of Parasitology.2013; 51(5): 589. CrossRef 10,093 View 88 Download Crossref PCR Diagnosis of Entamoeba histolytica Cysts in Stool Samples Joung-Ho Moon, Shin-Hyeong Cho, Jae-Ran Yu, Won-Ja Lee, Hyeng-Il Cheun Korean J Parasitol 2011;49(3):281-284. Published online September 30, 2011 DOI: https://doi.org/10.3347/kjp.2011.49.3.281 Abstract PDF Amebiasis is a protozoan disease caused by Entamoeba histolytica and a potential health threat in areas where sanitation and hygiene are inappropriate. Highly sensitive PCR methods for detection of E. histolytica in clinical and environmental samples are extremely useful to control amebiasis and to promote public health. The present study compared several primer sets for small subunit (SSU) rDNA and histone genes of E. histolytica cysts. A 246 bp of the SSU rDNA gene of pure cysts contained in phosphate-buffered saline (PBS) and in stool samples was successfully amplified by nested PCR, using the 1,147-246 bp primer set, of the primary PCR products which were pre-amplified using the 1,147 bp primer as the template. The detection limit of the nested PCR using the 1,147-246 primer set was 10 cysts in both groups (PBS and stool samples). The PCR to detect histone gene showed negative results. We propose that the nested PCR technique to detect SSU rDNA can be used as a highly sensitive genetic method to detect E. histolytica cysts in stool samples. Citations Citations to this article as recorded by Practical Guidance for Clinical Microbiology Laboratories: Laboratory Diagnosis of Parasites from the Gastrointestinal Tract Lynne S. Garcia, Michael Arrowood, Evelyne Kokoskin, Graeme P. Paltridge, Dylan R. Pillai, Gary W. Procop, Norbert Ryan, Robyn Y. Shimizu, Govinda Visvesvara Clinical Microbiology Reviews.2018;[Epub] CrossRef Evaluation of recombinant multi-epitope proteins for diagnosis of goat schistosomiasis by enzyme-linked immunosorbent assay Chao Lv, Yang Hong, Zhiqiang Fu, Ke Lu, Xiaodan Cao, Tao Wang, Chuangang Zhu, Hao Li, Rui Xu, Bingguang Jia, Qian Han, Xuefeng Dou, Yuanxi Shen, Zuhang Zhang, Jinli Zai, Jintao Feng, Jiaojiao Lin Parasites & Vectors.2016;[Epub] CrossRef Frequency of amoebiasis and other intestinal parasitoses in a settlement in Ilhéus City, State of Bahia, Brazil Helena Lúcia Carneiro Santos, Luci Ana Fernandes Martins, Regina Helena Saramago Peralta, José Mauro Peralta, Heloisa Werneck de Macedo Revista da Sociedade Brasileira de Medicina Tropical.2014; 47(1): 101. CrossRef 10,214 View 103 Download Crossref