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Kwang Hyun Shin	2 Articles
IgE Binding Reactivity of Peptide Fragments of Bla g 4, a Major German Cockroach Allergen Kwang Hyun Shin, Kyoung Yong Jeong, Chein-Soo Hong, Tai-Soon Yong Korean J Parasitol 2009;47(1):31-36. Published online March 12, 2009 DOI: https://doi.org/10.3347/kjp.2009.47.1.31 Abstract PDF Cockroaches have been recognized as a major cause of asthma. Bla g 4 is one of the most important German cockroach allergens. The aim of this study is to investigate IgE reactivity to the recombinant Bla g 4 (rBla g 4) in the sera of allergic patients and identify linear IgE binding epitope. For protein expression, full-length Bla g 4 (EF202172) was divided into 5 overlapping peptide fragments (E1: aa 1-100, E2: aa 34-77, E3: aa 74-117, E4: aa 114-156, and E5: aa 153-182). The full-length and 5 peptide fragments of Bla g 4 was generated by PCR and over-expressed in E. coli BL21 (DE3). The IgE binding reactivities of the full-length and peptide fragments were measured by ELISA using 32 serum samples of cockroach allergy. The sera of 8 patients (25%) reacted with rBla g 4. Four sera (100%) showed IgE-binding reactivity to full-length and peptide fragment 4, and 2 sera (50%) reacted with peptide fragment 2. One (20%) serum reacted with peptide fragment 3. The results of ELISA using overlapping recombinant fragments indicated that the epitope region was located at amino acid sequences 34-73 and 78-113, and major IgE epitope of Bla g 4 was located at amino acid sequences 118-152 of C-terminal. B-cell epitope analysis of German cockroach allergen Bla g 4 could contribute to the strategic development of more specific and potentially efficacious immunotherapy. Citations Citations to this article as recorded by Variability in German Cockroach Extract Composition Greatly Impacts T Cell Potency in Cockroach-Allergic Donors Giovanni Birrueta, April Frazier, Anna Pomés, Jill Glesner, Stephanie Filep, Coby Schal, Kyoung Yong Jeong, Curtis McMurtrey, Thomas Vander Schans, William H. Hildebrand, Paula Busse, Avraham Beigelman, Leonard B. Bacharier, Bjoern Peters, Alessandro Sett Frontiers in Immunology.2019;[Epub] CrossRef Insect (food) allergy and allergens Steffie de Gier, Kitty Verhoeckx Molecular Immunology.2018; 100: 82. CrossRef Cockroach allergen exposure and risk of asthma D. C. Do, Y. Zhao, P. Gao Allergy.2016; 71(4): 463. CrossRef IgE-Binding Epitope Mapping and Tissue Localization of the Major American Cockroach Allergen Per a 2 Mey-Fann Lee, Chia-Wei Chang, Pei-Pong Song, Guang-Yuh Hwang, Shyh-Jye Lin, Yi-Hsing Chen Allergy, Asthma & Immunology Research.2015; 7(4): 376. CrossRef Different Bla‐g T cell antigens dominate responses in asthma versus rhinitis subjects M. B. C. Dillon, V. Schulten, C. Oseroff, S. Paul, L. M. Dullanty, A. Frazier, X. Belles, M.‐D. Piulachs, C. Visness, L. Bacharier, G. R. Bloomberg, P. Busse, J. Sidney, B. Peters, A. Sette Clinical & Experimental Allergy.2015; 45(12): 1856. CrossRef The major cockroach allergen Bla g 4 binds tyramine and octopamine Lesa R. Offermann, Siew Leong Chan, Tomasz Osinski, Yih Wan Tan, Fook Tim Chew, J. Sivaraman, Yu-Keung Mok, Wladek Minor, Maksymilian Chruszcz Molecular Immunology.2014; 60(1): 86. CrossRef Environmental assessment and exposure reduction of cockroaches: A practice parameter Jay Portnoy, Ginger L. Chew, Wanda Phipatanakul, P. Brock Williams, Carl Grimes, Kevin Kennedy, Elizabeth C. Matsui, J. David Miller, David Bernstein, Joann Blessing-Moore, Linda Cox, David Khan, David Lang, Richard Nicklas, John Oppenheimer, Christopher Journal of Allergy and Clinical Immunology.2013; 132(4): 802. CrossRef Identification of Novel Allergenic Components from German Cockroach Fecal Extract by a Proteomic Approach Kyoung Yong Jeong, Chung-ryul Kim, Jina Park, In-Soo Han, Jung-Won Park, Tai-Soon Yong International Archives of Allergy and Immunology.2013; 161(4): 315. CrossRef The Cockroach and Allergic Diseases Myung Hyun Sohn, Kyu-Earn Kim Allergy, Asthma & Immunology Research.2012; 4(5): 264. CrossRef Der-p2 (Dermatophagoides pteronyssinus) allergen-like protein from the hard tickIxodes ricinus– a novel member of ML (MD-2-related lipid-recognition) domain protein family J. HORÁČKOVÁ, N. RUDENKO, M. GOLOVCHENKO, L. GRUBHOFFER Parasitology.2010; 137(7): 1139. CrossRef Household Arthropod Allergens in Korea Tai-Soon Yong, Kyoung Yong Jeong The Korean Journal of Parasitology.2009; 47(Suppl): S143. CrossRef 9,872 View 79 Download Crossref Reactivity of German Cockroach Allergen, Bla g 2, Peptide Fragments to IgE Antibodies in Patients' Sera Haeseok Lee, Kyoung Yong Jeong, Kwang Hyun Shin, Myung-hee Yi, Darambazar Gantulaga, Chein-Soo Hong, Tai-Soon Yong Korean J Parasitol 2008;46(4):243-246. Published online December 20, 2008 DOI: https://doi.org/10.3347/kjp.2008.46.4.243 Abstract PDF Bla g 2 is a cockroach allergen of great importance. This study was conducted to identify IgE-binding epitope(s) of Bla g 2 using the recombinant protein technique. Approximately 50% of tested sera showed IgE reactivity to Pichia-expressed Bla g 2 (PrBla g 2) and E. coli-expressed Bla g 2 (ErBla g 2). Only 5.3% of serum samples showed stronger reactivity to PrBla g 2 than ErBla g 2, indicating that serum was reactive to conformational or carbohydrate epitopes. The full-length and 5 peptide fragments of Bla g 2 were produced in E. coli. All fragments showed IgE-binding activity to the cockroach-allergy patients' sera. Specifically, peptide fragments of amino acid residue 1-75 and 146-225 appeared to be important for IgE-binding. The information about the IgE-binding epitope of Bla g 2 can aid in the diagnosis and treatment for cockroach allergies. Citations Citations to this article as recorded by Cross-reactive IgE responses to cockroach, house dust mite, and seafood allergens in patients with allergic rhinitis Sakinah Mohamad, V Sha Kri Eh Dam, Asma Abdullah Nurul, Fook Tim Chew, Baharudin Abdullah Expert Review of Clinical Immunology.2025; 21(9): 1307. CrossRef Edible insects as ingredients in food products: nutrition, functional properties, allergenicity of insect proteins, and processing modifications Jing Yang, Shuling Zhou, Hong Kuang, Chunhong Tang, Jiajia Song Critical Reviews in Food Science and Nutrition.2024; 64(28): 10361. CrossRef Cockroach allergen exposure and risk of asthma D. C. Do, Y. Zhao, P. Gao Allergy.2016; 71(4): 463. CrossRef Pulmonary α-1,3-Glucan–Specific IgA-Secreting B Cells Suppress the Development of Cockroach Allergy Preeyam S Patel, R Glenn King, John F Kearney The Journal of Immunology.2016; 197(8): 3175. CrossRef IgE-Binding Epitope Mapping and Tissue Localization of the Major American Cockroach Allergen Per a 2 Mey-Fann Lee, Chia-Wei Chang, Pei-Pong Song, Guang-Yuh Hwang, Shyh-Jye Lin, Yi-Hsing Chen Allergy, Asthma & Immunology Research.2015; 7(4): 376. CrossRef Different Bla‐g T cell antigens dominate responses in asthma versus rhinitis subjects M. B. C. Dillon, V. Schulten, C. Oseroff, S. Paul, L. M. Dullanty, A. Frazier, X. Belles, M.‐D. Piulachs, C. Visness, L. Bacharier, G. R. Bloomberg, P. Busse, J. Sidney, B. Peters, A. Sette Clinical & Experimental Allergy.2015; 45(12): 1856. CrossRef Preparation and Characterization of an Extract of German Cockroach From a Korean Source Kyoung Yong Jeong, Soo-Young Choi, Jae-Hyun Lee, Joo-Shil Lee, Tai-Soon Yong, Chein-Soo Hong, Jung-Won Park Allergy, Asthma & Immunology Research.2013; 5(2): 102. CrossRef Identification of Novel Allergenic Components from German Cockroach Fecal Extract by a Proteomic Approach Kyoung Yong Jeong, Chung-ryul Kim, Jina Park, In-Soo Han, Jung-Won Park, Tai-Soon Yong International Archives of Allergy and Immunology.2013; 161(4): 315. CrossRef The Cockroach and Allergic Diseases Myung Hyun Sohn, Kyu-Earn Kim Allergy, Asthma & Immunology Research.2012; 4(5): 264. CrossRef Validation of a Phage Display and Computational Algorithm by Mapping a Conformational Epitope of Bla g 2 Ruby Tiwari, Surendra S. Negi, Benjamin Braun, Werner Braun, Anna Pomés, Martin D. Chapman, Randall M. Goldblum, Terumi Midoro-Horiuti International Archives of Allergy and Immunology.2012; 157(4): 323. CrossRef Sensitization to per a 2 of the American cockroach correlates with more clinical severity among airway allergic patients in Taiwan Mey-Fann Lee, Pei-Peng Song, Guang-Yuh Hwang, Shyh-Jye Lin, Yi-Hsing Chen Annals of Allergy, Asthma & Immunology.2012; 108(4): 243. CrossRef Household Arthropod Allergens in Korea Tai-Soon Yong, Kyoung Yong Jeong The Korean Journal of Parasitology.2009; 47(Suppl): S143. CrossRef 8,566 View 86 Download Crossref