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Kyoung-Ju Song	7 Articles
Calpains are Involved in Entamoeba histolytica-Induced Death of HT-29 Colonic Epithelial Cells Yun Soo Jang, Kyoung-Ju Song, Ju Young Kim, Young Ah Lee, Kyeong Ah Kim, Sang Kyou Lee, Myeong Heon Shin Korean J Parasitol 2011;49(2):177-180. Published online June 14, 2011 DOI: https://doi.org/10.3347/kjp.2011.49.2.177 Abstract PDF Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. In this study, we investigated whether calpains are involved in the E. histolytica-induced cell death of HT-29 colonic epithelial cells. When HT-29 cells were co-incubated with E. histolytica, the propidium iodide stained dead cells markedly increased compared to that in HT-29 cells incubated with medium alone. This pro-death effect induced by ameba was effectively blocked by pretreatment of HT-29 cells with the calpain inhibitor, calpeptin. Moreover, knockdown of m- and ?-calpain by siRNA significantly reduced E. histolytica-induced HT-29 cell death. These results suggest that m- and ?-calpain may be involved in colon epithelial cell death induced by E. histolytica. Citations Citations to this article as recorded by Involvement of NOX2-derived ROS in human hepatoma HepG2 cell death induced by Entamoeba histolytica Young Ah Lee, Myeong Heon Shin Parasites, Hosts and Diseases.2023; 61(4): 388. CrossRef Signaling Role of NADPH Oxidases in ROS-Dependent Host Cell Death Induced by Pathogenic Entamoeba histolytica Young Ah Lee, Seobo Sim, Kyeong Ah Kim, Myeong Heon Shin The Korean Journal of Parasitology.2022; 60(3): 155. CrossRef Infection Strategies of Intestinal Parasite Pathogens and Host Cell Responses Bruno M. Di Genova, Renata R. Tonelli Frontiers in Microbiology.2016;[Epub] CrossRef Degradation of the Transcription Factors NF-κB, STAT3, and STAT5 Is Involved in Entamoeba histolytica-Induced Cell Death in Caco-2 Colonic Epithelial Cells Kyeong Ah Kim, Arim Min, Young Ah Lee, Myeong Heon Shin The Korean Journal of Parasitology.2014; 52(5): 459. CrossRef Entamoeba histolytica Induces Cell Death of HT29 Colonic Epithelial Cells via NOX1-Derived ROS Kyeong Ah Kim, Ju Young Kim, Young Ah Lee, Arim Min, Young Yil Bahk, Myeong Heon Shin The Korean Journal of Parasitology.2013; 51(1): 61. CrossRef Mechanisms of Adherence, Cytotoxicity and Phagocytosis Modulate the Pathogenesis of Entamoeba Histolytica Nathaniel CV Christy, William A Petri Future Microbiology.2011; 6(12): 1501. CrossRef 7,752 View 57 Download Crossref Evaluation of α-Tubulin as an Antigenic and Molecular Probe to Detect Giardia lamblia Juri Kim, Myeong Heon Shin, Kyoung-Ju Song, Soon-Jung Park Korean J Parasitol 2009;47(3):287-291. Published online August 28, 2009 DOI: https://doi.org/10.3347/kjp.2009.47.3.287 Abstract PDF The α/β-tubulin heterodimer is the basic subunit of microtubules in eukaryotes. Polyclonal antibodies specific to recombinant α-tubulin of Giardia lamblia were made, and found effective as a probe to specifically detect G. lamblia by immunofluorescence assays. Nucleotide sequences of α-tubulin genes were compared between G. lamblia WB and GS strains, prototypes of assemblage A and assemblage B, respectively. A set of primers was designed and used to amplify a portion of the α-tubulin gene from G. lamblia. PCR-RFLP analysis of this α-tubulin PCR product successfully differentiated G. lamblia into 2 distinct groups, assemblages A and B. The results indicate that α-tubulin can be used as a molecular probe to detect G. lamblia. Citations Citations to this article as recorded by Genetic diversity and molecular diagnosis of Giardia Yankai Chang, Junqiang Li, Longxian Zhang Infection, Genetics and Evolution.2023; 113: 105482. CrossRef Tubulin as a potential molecular target for resveratrol in Giardia lamblia trophozoites, in vitro and in silico approaches José Roberto Vargas-Villanueva, Filiberto Gutiérrez-Gutiérrez, Mariana Garza-Ontiveros, Sendar Daniel Nery-Flores, Lizeth Guadalupe Campos-Múzquiz, Dagoberto Vazquez-Obregón, Raul Rodriguez-Herrera, Lissethe Palomo-Ligas Acta Tropica.2023; 248: 107026. CrossRef Phosphorylation of Serine 148 in Giardia lamblia End‐binding 1 Protein is Important for Cell Division Juri Kim, Hye‐Yeon Lee, Kyu‐Ho Lee, Soon‐Jung Park Journal of Eukaryotic Microbiology.2017; 64(4): 464. CrossRef Host defences against Giardia lamblia G. Lopez‐Romero, J. Quintero, H. Astiazarán‐García, C. Velazquez Parasite Immunology.2015; 37(8): 394. CrossRef Characterization of Microtubule-Binding and Dimerization Activity of Giardia lamblia End-Binding 1 Protein Juri Kim, Sara Nagami, Kyu-Ho Lee, Soon-Jung Park, Ira J. Blader PLoS ONE.2014; 9(5): e97850. CrossRef Zoonotic potential of Giardia Una Ryan, Simone M. Cacciò International Journal for Parasitology.2013; 43(12-13): 943. CrossRef Identification of Antigenic Proteins in Trichomonas vaginalis Hye-Yeon Lee, Sujin Hyung, Jong Woong Lee, Juri Kim, Myeong Heon Shin, Jae-Sook Ryu, Soon-Jung Park The Korean Journal of Parasitology.2011; 49(1): 79. CrossRef 8,551 View 115 Download Crossref Seroprevalence of toxoplasmosis in Korean pregnant women Kyoung-Ju Song, Jong-Chul Shin, Ho-Joon Shin, Ho-Woo Nam Korean J Parasitol 2005;43(2):69-71. Published online June 20, 2005 DOI: https://doi.org/10.3347/kjp.2005.43.2.69 Abstract PDF This study was performed in order to evaluate the sero-epidemiological status of toxoplasmosis in pregnant Korean women. Among 5,175 sera and 750 amniotic fluid samples obtained from pregnant women, 41 serum samples (0.79%) and 10 (1.33%) amniotic fluid samples tested positive for IgG antibodies by ELISA. Fifty one cases showing a score more than 0.25 on ELISA were tested for PCR reaction against the SAG1 gene. Only one case of the 51 ELISA positive cases exhibited a positive reaction on all tests. This case had a history of acute nephropyelitis during early pregnancy, but fortunately, had delivered a phenotypically healthy baby. In this study, the seroprevalence of toxoplasmosis in pregnant women was found to be comparatively low, consistent with previous reports from Korea. However our trials, performed with a variety of diagnostic tools, were considered to be useful for the precise diagnosis of congenital toxoplasmosis. Citations Citations to this article as recorded by Global seroprevalence of Toxoplasma gondii in pregnant women: a systematic review and meta-analysis Nader Salari, Avijeh Rahimi, Hosna Zarei, Amir Abdolmaleki, Shabnam Rasoulpoor, Shamarina Shohaimi, Masoud Mohammadi BMC Pregnancy and Childbirth.2025;[Epub] CrossRef Toxoplasmosis as a Possible Cause of Chronic Granulomatous Tonsillitis: A Case Report Hae Won Choi, Min Kyu Park, Hyeon A Lee, Jae Ho Chung Journal of Clinical Otolaryngology Head and Neck Surgery.2022; 33(1): 30. CrossRef MIC17A is a novel diagnostic marker for feline toxoplasmosis Jinling Chen, Lilan Xue, Hongxia Hu, Xiaoyan Yin, Hui Cao, Bang Shen Animal Diseases.2022;[Epub] CrossRef Toxoplasmosis infection in newborn: A systematic review and meta-analysis Mehran Akbari, Davood Azadi, Danial Habibi, Sahar Khodashenas, Fakhreddin Shariatmadari, Behnam Abedi Advanced Biomedical Research.2022; 11(1): 75. CrossRef Seroprevalence of Toxoplasma gondii assayed using Rapid Diagnostic Tests among Residents in Three Counties Adjacent to The Demilitarized Zone, Korea Jeehi Jung, Jinyoung Lee, Yoon Kyung Chang, Seong Kyu Ahn, Seo Hye Park, Sung-Jong Hong, Jihoo Lee, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam, Tong-Soo Kim, Dongjae Kim The Korean Journal of Parasitology.2021; 59(1): 9. CrossRef Clusters of Toxoplasmosis in Ganghwa-gun, Cheorwon-gun, and Goseong-gun, Korea Jihye Yu, Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim The Korean Journal of Parasitology.2021; 59(3): 251. CrossRef Seroprevalence of Toxoplasma gondii and Toxocara spp. infections among pregnant women with and without previous abortions in the west of Iran Vahid Raissi, Ali Taghipour, Zahra Navi, Soudabeh Etemadi, Zahra Sohrabi, Nasrin Sohrabi, Muhammad Getso, Sina Shamsaei, Maryam Fasihi Karami, Omid Raiesi Journal of Obstetrics and Gynaecology Research.2020; 46(3): 382. CrossRef The global seroprevalence of anti-Toxoplasma gondii antibodies in women who had spontaneous abortion: A systematic review and meta-analysis Tooran Nayeri, Shahabeddin Sarvi, Mahmood Moosazadeh, Afsaneh Amouei, Zahra Hosseininejad, Ahmad Daryani, Maria Angeles Gómez-Morales PLOS Neglected Tropical Diseases.2020; 14(3): e0008103. CrossRef Seroprevalence and B1 gene Phylogeny of Toxoplasma gondii of Dogs and Cats in Republic of Korea Yeojin Park, Jinhyeong Noh, Hyun-Ji Seo, Keun-Ho Kim, Subin Min, Mi-Sun Yoo, Bo-Ram Yun, Jong-Ho Kim, Eun-Jin Choi, Doo-Sung Cheon, Sung-Jong Hong, Soon-Seek Yoon, Yun Sang Cho The Korean Journal of Parasitology.2020; 58(3): 257. CrossRef Seroprevalence of toxoplasma gondii infection: An umbrella review of updated systematic reviews and meta-analyses Vahid Rahmanian, Karamatollah Rahmanian, AbdolrezaSotoodeh Jahromi, Saied Bokaie Journal of Family Medicine and Primary Care.2020; 9(8): 3848. CrossRef Clusters of Toxoplasmosis in Gyodong-Myeon and Samsan-Myeon, Ganghwa-Gun, Korea Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim The Korean Journal of Parasitology.2020; 58(5): 493. CrossRef Distribution of Toxoplasma gondii IgM and IgG antibody seropositivity among age groups and gestational periods in pregnant women Shahida Sadiqui, Syed Rafiq Hussain Shah, Babiker Saad Almugadam, Qismat Shakeela, Shehzad Ahmad F1000Research.2019; 7: 1823. CrossRef Distribution of Toxoplasma gondii IgM and IgG antibody seropositivity among age groups and gestational periods in pregnant women Shahida Sadiqui, Syed Rafiq Hussain Shah, Babiker Saad Almugadam, Qismat Shakeela, Shehzad Ahmad F1000Research.2019; 7: 1823. CrossRef Congenital toxoplasmosis among Iranian neonates: a systematic review and meta-analysis Shahabeddin Sarvi, Tooran Nayeri Chegeni, Mehdi Sharif, Mahbobeh Montazeri, Seyed Abdollah Hosseini, Afsaneh Amouei, Zahra Hosseininejad, Davood Anvari, Reza Saberi, Shaban Gohardehi, Ahmad Daryani Epidemiology and Health.2019; 41: e2019021. CrossRef Status of common parasitic diseases in Korea in 2019 Sun Huh Journal of the Korean Medical Association.2019; 62(8): 437. CrossRef Seroprevalence of Toxoplasma gondii among pregnant women attending antenatal clinics at Hawassa University comprehensive specialized and Yirgalem General Hospitals, in Southern Ethiopia Demissie Assegu Fenta BMC Infectious Diseases.2019;[Epub] CrossRef Excretion of Toxoplasma gondii oocysts from Feral Cats in Korea Kyu-Sung Ahn, Ah-Jin Ahn, Sang-ik Park, Woon-Mok Sohn, Jae-han Shim, Sung-Shik Shin The Korean Journal of Parasitology.2019; 57(6): 665. CrossRef Seroprevalence of TORCH Infections in Pregnant Women Attending Antenatal Clinic in a Tertiary Care Hospital Kavitha Paul Konikkara, Irene Jose Manjiyil, Vimalraj Angattukuzhiyil Narayanan, Prithi Nair Kannambra Journal of Evolution of Medical and Dental Sciences.2019; 8(39): 2958. CrossRef Prevalence of Food-Borne Toxoplasma in Pregnant Women Population of Urmia, Iran A. Eshratkhah Mohammadnejad, G. Eslami, F. Shamsi, A. Pirnejad, A. Samie, J. Safabakhsh, A. Sakhavand, M. Elloumi Journal of Food Quality and Hazards Conrol.2018; 5(1): 17. CrossRef TORCH (toxoplasmosis, rubella, cytomegalovirus, and herpes simplex virus) screening of small for gestational age and intrauterine growth restricted neonates: efficacy study in a single institute in Korea Mi Hae Chung, Chan Ok Shin, Juyoung Lee Korean Journal of Pediatrics.2018; 61(4): 114. CrossRef Genotyping of Toxoplasma gondii Isolated from cat Feces in Songkhla, Southern Thailand Waenurama Chemoh, Nongyao Sawangjaroen, Veeranoot Nissapatorn, Natthawan Sermwittayawong Veterinary Parasitology: Regional Studies and Reports.2018; 13: 105. CrossRef An outbreak of toxoplasmosis in squirrel monkeys (Saimiri sciureus) in South Korea Hanseul Oh, Kyung‐Yeon Eo, Sanjeev Gumber, Jung Joo Hong, C‐Yoon Kim, Hyun‐Ho Lee, Young‐Mok Jung, Jin Kim, Gyu‐Whan Whang, Ji‐Min Lee, Yong‐Gu Yeo, Bokyeong Ryu, Ji‐Sook Ryu, Seul‐Kee Lee, Ukjin Kim, Sin‐Geun Kang, Jae‐Hak Park Journal of Medical Primatology.2018; 47(4): 238. CrossRef Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets Won Gi Yoo, Sun-Min Kim, Eun Jeong Won, Ji-Yun Lee, Fuhong Dai, Ho Choon Woo, Ho-Woo Nam, Tae Im Kim, Jeong-Hee Han, Dongmi Kwak, Yun Sang Cho, Seung-Won Kang, Tong-Soo Kim, Xing-Quan Zhu, Chunren Wang, Heejeong Youn, Sung-Jong Hong The Korean Journal of Parasitology.2018; 56(5): 437. CrossRef Distribution of Toxoplasma gondii IgM and IgG antibody seropositivity among age groups and gestational periods in pregnant women Shahida Sadiqui, Syed Rafiq Hussain Shah, Babiker Saad Almugadam, Qismat Shakeela, Shehzad Ahmad F1000Research.2018; 7: 1823. CrossRef Toxoplasmose et cancer : connaissances actuelles et perspectives de recherche M. Vittecoq, F. Thomas Bulletin de la Société de pathologie exotique.2017; 110(1): 76. CrossRef Seroprevalence and potential risk factors of T. gondii infection in pregnant women attending antenatal care at Bonga Hospital, Southwestern Ethiopia Jalel Negero, Moti Yohannes, Kifle Woldemichael, Dechassa Tegegne International Journal of Infectious Diseases.2017; 57: 44. CrossRef Toxoplasma gondii survey in cats from two environments of the city of Rio de Janeiro, Brazil by Modified Agglutination Test on sera and filter-paper Paula F. Bolais, Philippe Vignoles, Pamela F. Pereira, Rafael Keim, Abdelkrim Aroussi, Khadja Ismail, Marie-Laure Dardé, Maria Regina Amendoeira, Aurélien Mercier Parasites & Vectors.2017;[Epub] CrossRef Toxoplasmosis seroprevalence in Iranian women and risk factors of the disease: a systematic review and meta-analysis Azadeh Mizani, Abbas Alipour, Mehdi Sharif, Shahabeddin Sarvi, Afsaneh Amouei, Azar Shokri, Mohammad-Taghi Rahimi, Seyed Abdollah Hosseini, Ahmad Daryani Tropical Medicine and Health.2017;[Epub] CrossRef Seroprevalence of Toxoplasmosis with ELISA and Rapid Diagnostic Test among Residents in Gyodong-do, Inchon city, Korea: A Four-Year Follow-up Yeong Hoon Kim, Ji hoo Lee, Seong kyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam The Korean Journal of Parasitology.2017; 55(3): 247. CrossRef Seroprevalence of Toxoplasmosis Detected by RDT in Residents near the DMZ (demilitarized zone) of Cheorwon-gun, Gangwon-do, Korea Yeong Hoon Kim, Jihoo Lee, Young-Eun Kim, Seongkyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam The Korean Journal of Parasitology.2017; 55(4): 385. CrossRef Some epidemiological and serological studies on schistosomiasis in Najran area, Saudi Arabia I. S. El-Shahawy, M. I. Khalil, A. S. Adail Helminthologia.2016; 53(2): 172. CrossRef Serological study of toxoplasmosis in women with previous history of abortion at Hamedan’s medical centers during 2012–2013 M. Abedi, H. Heidari, Z. Sadeghi Dehkordi, M. R. Youssefi Comparative Clinical Pathology.2015; 24(3): 589. CrossRef Seroprevalence of Toxoplasma gondii infection and associated risk factors among pregnant women in Debre Tabor, Northwest Ethiopia Birhan Agmas, Reta Tesfaye, Digsu Negese Koye BMC Research Notes.2015;[Epub] CrossRef Frequency of Toxoplasma Infection Among Pregnant Women and Their Newborn in Kashan, Iran Sima Rasti, Hossein Hooshyar, Mohsen Arbabi, Atefeh Fatahian, Mitra Behrashi, Ahmad Talebian, Mojgan Bandehpour, Seyed Gholamabbas Mousavi Zahedan Journal of Research in Medical Sciences.2015;[Epub] CrossRef Toxoplasma gondiiInfection in Pregnant Women: A Seroprevalence and Case-Control Study in Eastern China Wei Cong, Xiao-Yan Dong, Qing-Feng Meng, Na Zhou, Xiang-Yang Wang, Si-Yang Huang, Xing-Quan Zhu, Ai-Dong Qian BioMed Research International.2015; 2015: 1. CrossRef Seroprevalence of Coxiella burnetii and Toxoplasam gondii in cattle in Seoul, Korea Neung-Hee Kim, Hye-Ra Kim, Hyung-Suk Park, Young-sub Kim, Ju-Hyung Lee Korean Journal of Veterinary Service.2015; 38(4): 233. CrossRef Mesial Temporal Lobe Epilepsy in Congenital Toxoplasmosis: A Case Report Woo Kyo Jeong, Byung-Euk Joo, Ji-hye Seo, Jun Kyu Mun, Juhyeon Kim, Dae-Won Seo Journal of Epilepsy Research.2015; 5(1): 25. CrossRef Seroprevalence of Toxoplasma gondii in the Iranian general population: A systematic review and meta-analysis Ahmad Daryani, Shahabeddin Sarvi, Mohsen Aarabi, Azadeh Mizani, Ehsan Ahmadpour, Azar Shokri, Mohammad-Taghi Rahimi, Mehdi Sharif Acta Tropica.2014; 137: 185. CrossRef Seroprevalence and Risk Factors for Toxoplasma gondii Infection among Pregnant Women in Northeast Iran Jalal Babaie, Samira Amiri, Ehsan Mostafavi, Nayereh Hassan, Peyman Lotfi, Ahmad Reza Esmaeili Rastaghi, Majid Golkar Clinical and Vaccine Immunology.2013; 20(11): 1771. CrossRef Seroprevalence of Toxoplasmosis in the Residents of Cheorwon-gun, Gangwon-do, Korea Hye-Jin Ahn, Pyo Yun Cho, Seong Kyu Ahn, Tong-Soo Kim, Chom-Kyu Chong, Sung-Jong Hong, Seok Ho Cha, Ho-Woo Nam The Korean Journal of Parasitology.2012; 50(3): 225. CrossRef Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea Hyemi Lim, Sang-Eun Lee, Bong-Kwang Jung, Min-Ki Kim, Mi Youn Lee, Ho-Woo Nam, Jong-Gyun Shin, Cheong-Ha Yun, Han-Ik Cho, Eun-Hee Shin, Jong-Yil Chai The Korean Journal of Parasitology.2012; 50(4): 287. CrossRef A Surge in the Seroprevalence of Toxoplasmosis among the Residents of Islands in Gangwha-gun, Incheon, Korea Zhaoshou Yang, Pyo Yun Cho, Seong Kyu Ahn, Hye-Jin Ahn, Tong-Soo Kim, Chom-Kyu Chong, Sung-Jong Hong, Seok Ho Cha, Ho-Woo Nam The Korean Journal of Parasitology.2012; 50(3): 191. CrossRef Prevalence of Toxoplasma gondii infection in antenatal population in Menoufia governorate, Egypt Hala K. El Deeb, Hesham Salah-Eldin, Seham Khodeer, Azza Abdu Allah Acta Tropica.2012; 124(3): 185. CrossRef Epidemiology of Ocular Toxoplasmosis Eskild Petersen, Aize Kijlstra, Miles Stanford Ocular Immunology and Inflammation.2012; 20(2): 68. CrossRef Seroprevalence of Toxoplasmosis in Pregnant Women in Daejeon, Korea Young Hyun Ko, Mina Lee, So Young Shin, Sun Hoe Koo, Jeong Hoon Song, Jinsook Lim, Kye Chul Kwon Laboratory Medicine Online.2011; 1(4): 190. CrossRef Maintained Seroprevalence of Toxoplasmosis among the Residents of Jeju Island, Korea Sung-Jong Hong, Chom-Kyu Chong, Kyutaeg Lee, Tong-Soo Kim, Yeon-Pyo Hong, Hye-Jin Ahn, Hak-Yong Kim, A-Ra Ko, Yong-Joo Kim, Ho-Woo Nam The Korean Journal of Parasitology.2011; 49(3): 309. CrossRef A Case of Cerebral Toxoplasmosis Following Tandem Autologous Stem Cell Transplantation in a Multiple Myeloma Patient Kye-Hyung Kim, Kyoung-Ho Song, Jae Hyun Jeon, Wan Beom Park, Sang-Won Park, Hong Bin Kim, Nam Joong Kim, Inho Kim, Myoung-don Oh Infection and Chemotherapy.2010; 42(3): 181. CrossRef What Do We Know About Risk Factors for Infection in Humans withToxoplasma gondiiand How Can We Prevent Infections? E. Petersen, G. Vesco, S. Villari, W. Buffolano Zoonoses and Public Health.2010; 57(1): 8. CrossRef Spectrum of Intracranial Parenchymal Lesions in Patients with Human Immunodeficiency Virus Infection in the Republic of Korea Pyoeng Gyun Choe, Wan Beom Park, Jin Su Song, Kyoung-Ho Song, Jae Hyun Jeon, Sang-Won Park, Hong Bin Kim, Kee-Hyun Chang, Myoung-don Oh, Kang Won Choe, Nam Joong Kim Journal of Korean Medical Science.2010; 25(7): 1005. CrossRef Toxoplasmosis among the pregnant women attending a Saudi maternity hospital: seroprevalence and possible risk factors H. I. AL Mohammad, T. T. Amin, M. H. Balaha, M. S. AL Moghannum Annals of Tropical Medicine & Parasitology.2010; 104(6): 493. CrossRef Toxoplasmosis snapshots: Global status of Toxoplasma gondii seroprevalence and implications for pregnancy and congenital toxoplasmosis Georgios Pappas, Nikos Roussos, Matthew E. Falagas International Journal for Parasitology.2009; 39(12): 1385. CrossRef Seroprevalence of Toxoplasma gondii Infection and Characteristics of Seropositive Patients in General Hospitals in Daejeon, Korea Dae-Whan Shin, Dong-Yeub Cha, Quan Juan Hua, Guang-Ho Cha, Young-Ha Lee The Korean Journal of Parasitology.2009; 47(2): 125. CrossRef Nested PCR-based detection of Toxoplasma gondii in German shepherd dogs and stray cats in South Korea J.Y. Lee, S.E. Lee, E.G. Lee, K.H. Song Research in Veterinary Science.2008; 85(1): 125. CrossRef Genotyping of a Korean isolate of Toxoplasma gondii by multilocus PCR-RFLP and microsatellite analysis Juan-Hua Quan, Tae Yun Kim, In-Uk Choi, Young-Ha Lee The Korean Journal of Parasitology.2008; 46(2): 105. CrossRef Seroprevalence of Toxoplasma gondii Infection and Risk Factors Associated with Seropositivity of Pregnant Women in Korea Kyun Han, Dae-Whan Shin, Tae-Yong Lee, Young-Ha Lee Journal of Parasitology.2008; 94(4): 963. CrossRef Seroepidemiology of Toxoplasma gondii infection in pregnant women in a public hospital in northern Mexico Cosme Alvarado-Esquivel, Antonio Sifuentes-Álvarez, Sergio Guadalupe Narro-Duarte, Sergio Estrada-Martínez, Juan Humberto Díaz-García, Oliver Liesenfeld, Sergio Arturo Martínez-García, Arturo Canales-Molina BMC Infectious Diseases.2006;[Epub] CrossRef 10,018 View 128 Download Crossref Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against experimental Neospora caninum infection Jung-Hwa Cho, Woo-Suk Chung, Kyoung-Ju Song, Byoung-Kuk Na, Seung-Won Kang, Chul-Yong Song, Tong-Soo Kim Korean J Parasitol 2005;43(1):19-25. Published online March 20, 2005 DOI: https://doi.org/10.3347/kjp.2005.43.1.19 Abstract PDF Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against N. caninum infection was evaluated in vitro and in vivo. Two major immunodominant surface antigens (NcSAG1 and NcSRS2) and two dense granule proteins (NcDG1 and NcDG2) of N. caninum tachyzoites were expressed in E. coli, respectively. An in vitro neutralization assay using polyclonal antisera raised against each recombinant antigen showed inhibitory effects on the invasion of N. caninum tachyzoites into host cells. Separate groups of gerbils were immunized with the purified recombinant proteins singly or in combinations and animals were then challenged with N.caninum. Following these experimental challenges, the protective efficacy of each vaccination was determined by assessing animal survival rate. All experimental groups showed protective effects of different degrees against experimental infection. The highest protection efficacy was observed for combined vaccination with NcSRS2 and NcDG1. Our results indicate that combined vaccination with the N. caninum recombinant antigens, NcSRS2 and NcDG1, induces the highest protective effect against N. caninum infection in vitro and in vivo. Citations Citations to this article as recorded by Molecular characterization of Neospora caninum major antigens NcSAG1 and NcSRS2 Soledad Echeverría, Federico Carrión, Martín Soñora, Andrés Cabrera, Carlos Robello Royal Society Open Science.2025;[Epub] CrossRef A Listeria monocytogenes-Based Vaccine Formulation Reduces Vertical Transmission and Leads to Enhanced Pup Survival in a Pregnant Neosporosis Mouse Model Dennis Imhof, William Robert Pownall, Camille Monney, Anna Oevermann, Andrew Hemphill Vaccines.2021; 9(12): 1400. 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CrossRef Immunization with native surface protein NcSRS2 induces a Th2 immune response and reduces congenital Neospora caninum transmission in mice G.J. Haldorson, B.A. Mathison, K. Wenberg, P.A. Conrad, J.P. Dubey, A.J. Trees, I. Yamane, T.V. Baszler International Journal for Parasitology.2005; 35(13): 1407. CrossRef 9,128 View 94 Download Crossref Decreasing effect of an anti-Nfa1 polyclonal antibody on the in vitro cytotoxicity of pathogenic Naegleria fowleri Seok-Ryoul Jeong, Su-Yeon Kang, Sang-Chul Lee, Kyoung-Ju Song, Kyung-il Im, Ho-Joon Shin Korean J Parasitol 2004;42(1):35-40. Published online March 20, 2004 DOI: https://doi.org/10.3347/kjp.2004.42.1.35 Abstract PDF The nfa1 gene was cloned from a cDNA library of pathogenic Naegleria fowleri by immunoscreening; it consisted of 360 bp and produced a 13.1 kDa recombinant protein (rNfa1) that showed the pseudopodia-specific localization by immunocytochemistry in the previous study. Based on the idea that the pseudopodia-specific Nfa1 protein mentioned above seems to be involved in the pathogenicity of N. fowleri, we observed the effect of an anti-Nfa1 antibody on the proliferation of N. fowleri trophozoites and the cytotoxicity of N. fowleri trophozoites on the target cells. The proliferation of N. fowleri trophozoites was inhibited after being treated with an anti-Nfa1 polyclonal antibody in a dose-dependent manner for 48 hrs. By a light microscope, CHO cells co-cultured with N. fowleri trophozoites (group I) for 48 hrs showed severe morphological destruction. On the contrary, CHO cells co-cultured with N. fowleri trophozoites and anti-Nfa1 polyclonal antibody (1:100 dilution) (group II) showed less destruction. In the LDH release assay results, group I showed 50.6% cytotoxicity, and group II showed 39.3%. Consequently, addition of an anti-Nfa1 polyclonal antibody produced a decreasing effect of in vitro cytotoxicity of N. fowleri in a dosedependent manner. Citations Citations to this article as recorded by A review of the mechanism, diagnosis, and treatment of Naegleria fowleri infection Ling Dai, Xin-Ru Guo, Xu-Rui Chen, Ming-Hao Ma, Zi-Han Liu, Juan Lai, Jun Lu, Ming Feng, Xi-Xia Liu, Sheng-Hui Yang Frontiers in Microbiology.2025;[Epub] CrossRef Understanding the pathogenicity of Naegleria fowleri in association with N. fowleri antigen-1 (Nfa1) Jong-Hyun Kim, Hae-Jin Sohn, Ho-Joon Shin, Stacy E. Walz, Suk-Yul Jung Parasites, Hosts and Diseases.2024; 62(4): 385. CrossRef Role of cathepsin B of Naegleria fowleri during primary amebic meningoencephalitis Itzel Berenice Rodríguez-Mera, María Maricela Carrasco-Yépez, Ismael Vásquez-Moctezuma, José Correa-Basurto, Gema Ramírez- Salinas, Diego Arturo Castillo-Ramírez, Érika Rosales-Cruz, Saúl Rojas-Hernández Parasitology Research.2022; 121(11): 3287. CrossRef Opinion: Iron, Climate Change and the ‘Brain Eating Amoeba’ Naegleria fowleri Sutherland K. Maciver, Paul J. McLaughlin, David K. 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Published online September 20, 2003 DOI: https://doi.org/10.3347/kjp.2003.41.3.165 Abstract PDF This study describes the characterization of 80 kDa protease showing gelationlytic property among three proteases in the excretory/secretory proteins (ESP) from Toxoplasma gondii. The protease activity was detected in the ESP but not in the somatic extract of RH tachyzoites. This protease was active only in the presence of calcium ion but not other divalent cationic ions such as Cu²⁺, Zn²⁺, Mg²⁺, and Mn²⁺, implying that Ca²⁺ is critical factor for the activation of the protease. The 80 kDa protease was optimally active at pH 7.5. Its gelatinolytic activity was maximal at 37℃, and significant level of enzyme activity of the protease remained after heat treatment at 56℃ for 30 min or 100℃ for 10 min. This thermostable enzyme was strongly inhibited by metal chelators, i.e., EDTA, EGTA, and 1,10-phenanthroline. Thus, the 80 kDa protease n the ESP secreted by T. gondii was classified as a calcium dependent neutral metalloprotease. Citations Citations to this article as recorded by Purification and characterization of cysteine protease of Sarcocystis fusiformis from infected Egyptian water buffaloes Amal Z. Barakat, Azza M. Abdel-Aty, Marwa K. Ibrahim, Hala A. Salah, Usama M. Hegazy, Rasha A. M. Azouz, Roqaya I. Bassuiny, Raafat M. Shaapan, Saleh A. Mohamed Scientific Reports.2023;[Epub] CrossRef In vitro activity of N-phenyl-1,10-phenanthroline-2-amines against tachyzoites and bradyzoites of Toxoplasma gondii Erica S. Martins-Duarte, Juliana de Araujo Portes, Ramon Borges da Silva, Hugo Silva Pires, Simon J. Garden, Wanderley de Souza Bioorganic & Medicinal Chemistry.2021; 50: 116467. CrossRef Characterization of metalloproteases and serine proteases of Toxoplasma gondii tachyzoites and their effect on epithelial cells Carlos J. Ramírez-Flores, Rosalba Cruz-Mirón, Rossana Arroyo, Mónica E. 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CrossRef 8,585 View 68 Download Crossref Molecular cloning and characterization of peroxiredoxin from Toxoplasma gondii Eui-Sun Son, Kyoung-Ju Song, Jong-Chul Shin, Ho-Woo Nam Korean J Parasitol 2001;39(2):133-141. Published online June 30, 2001 DOI: https://doi.org/10.3347/kjp.2001.39.2.133 Abstract PDF A cDNA of 1.1 kb comprising the gene encoding the peroxiredoxin of Toxoplasma gondii (TgPrx) has been cloned. The open reading frame of 591 bp was translated into a protein of 196 amino acids with a molecular mass of 25 kDa. Conserved 2 cysteine domains of Phe-Val-Cys-Pro and Glu-Val-Cys-Pro indicated TgPrx belonged to 2-Cys Prx families. TgPrx showed the highest homology with that of Arabidopsis thaliana by 53.9% followed by Entamoeba histolytica with 39.5% by the amino acid sequence alignment. Polyclonal antibody against recombinant TgPrx detected 25 kDa band in T. gondii without binding to host cell proteins. TgPrx was located in the cytoplasm of T. gondii extracellularly or intracellularly by immunofluorescence assay. The expression of TgPrx was increased as early as 30 min after the treatment with artemisinin in the intracellular stage, while no changes in those of host Prx I and TgSOD. This result implies that TgPrx may function as an antioxidant protecting the cell from the attack of reactive oxygen intermediates. It is also suggested that TgPrx is a possible target of chemotherapy. 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