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Kyung-Hee Choi	1 Article
Genetic analyses of Acanthamoeba isolates from contact lens storage cases of students in Seoul, Korea Hak-Sun Yu, Kyung-Hee Choi, Hyo-Kyung Kim, Hyun-Hee Kong, Dong-Il Chung Korean J Parasitol 2001;39(2):161-170. Published online June 30, 2001 DOI: https://doi.org/10.3347/kjp.2001.39.2.161 Abstract PDF We conducted both the small subunit ribosomal DNA (SSU rDNA) polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and mitochondrial (mt) DNA RFLP analyses for a genetic characterization of Acanthamoeba isolates from contact lens storage cases of students in Seoul, Korea. Twenty-three strains of Acanthamoeba from the American Type Culture Collection and twelve clinical isolates from Korean patients were used as reference strains. Thirty-nine isolates from contact lens storage cases were classified into seven types (KA/LS1, KA/LS2, KA/LS4, KA/LS5, KA/LS7, KA/LS18, KA/LS31). Four types (KA/LS1, KA/LS2, KA/LS5, KA/LS18) including 33 isolates were regarded as A. castellanii complex by riboprints. KA/LS1 type was the most predominant (51.3%) in the present survey area, followed by KA/LS2 (20.9%), and KA/LS5 (7.7%) types. Amoebae of KA/LS1 type had the same mtDNA RFLP and riboprint patterns as KA/E2 and KA/E12 strains, clinical isolates from Korean keratitis patients. Amoebae of KA/LS2 type had the identical mtDNA RFLP patterns with A. castellanii Ma strain, a corneal isolate from an American patient as amoebae of KA/LS5 type, with KA/E3 and KA/E8 strains from other Korean keratitis patients. Amoebae of KA/LS18 type had identical patterns with JAC/E1, an ocular isolate from a Japanese patient. Three types, which remain unidentified at species level, were not corresponded with any clinical isolate in their mtDNA RFLP and riboprint patterns. Out of 39 isolates analyzed in this study, mtDNA RFLP and riboprint patterns of 33 isolates (84.6%) were identical to already known clinical isolates, and therefore, they may be regarded as potentially keratopathogenic. These results suggest that contact lens wearers in Seoul should pay more attention to hygienic maintenance of contact lens storage cases for the prevention of Acanthamoeba keratitis. Citations Citations to this article as recorded by Parasitological and microbiological assessment of contact lens storage cases: a survey of asymptomatic lens student wearers from five medical specialties in Tunisia, North Africa Sameh Belgacem, Raja Chaâbane-Banaoues, Amira Mejri, Sawsen Ben Ifa, Maha Mastouri, Hamouda Babba BMC Infectious Diseases.2025;[Epub] CrossRef The amoebicidal effect of Torreya nucifera extract on Acanthamoeba lugdunensis Min Seung Kang, Sangyoon Kim, Da Som Kim, Hak Sun Yu, Ji Eun Lee, Yi Cao PLOS ONE.2023; 18(2): e0281141. CrossRef The First Case of Cutaneous Acanthamoebiasis Caused by Acanthamoeba triangularis in Korea Mi Soo Choi, Na Hye Myong, Min Seo, Sukbin Jang, Dae Kwan Yun, Kyujin Yeom, Dong-Il Chung, Byung Cheol Park, Yeonchul Hong, Myung Hwa Kim Annals of Dermatology.2023; 35(Suppl 2): S275. CrossRef Adhesion ofAcanthamoebaon Cosmetic Contact Lenses Seung-Mok Lee, Ji-Eun Lee, Da-In Lee, Hak-Sun Yu Journal of Korean Medical Science.2018;[Epub] CrossRef Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach Angela Magnet, Thiago Santos Gomes, Carmen Pardinas, Natalia Garcia de Blas, Cruz Sadaba, Eugenia Carrillo, Fernando Izquierdo, José Manuel Benítez del Castillo, Carolina Hurtado, Carmen del Aguila, Soledad Fenoy Parasites & Vectors.2018;[Epub] CrossRef Effect of Multipurpose Solution Combined With Autophagy Inhibitors on Adhesion of Acanthamoeba trophozoites to Silicone Hydrogel Contact Lenses Seung-Mok Lee, Da-In Lee, Sung-Hee Park, Hak-Sun Yu, Ji-Eun Lee, Jong-Soo Lee Cornea.2017; 36(12): 1538. CrossRef Effects of multipurpose solutions on the adhesion of Acanthamoeba to rigid gas permeable contact lenses Ga‐Hyun Lee, Hak‐Sun Yu, Ji‐Eun Lee Ophthalmic and Physiological Optics.2016; 36(2): 93. CrossRef Adhesion of Acanthamoeba on Silicone Hydrogel Contact Lenses Ga-Hyun Lee, Ji-Eun Lee, Mi-Kyung Park, Hak-Sun Yu Cornea.2016; 35(5): 663. CrossRef Acanthamoeba spp. in Contact Lenses from Healthy Individuals from Madrid, Spain Thiago dos Santos Gomes, Angela Magnet, Fernando Izquierdo, Lucianna Vaccaro, Fernando Redondo, Sara Bueno, Maria Luisa Sánchez, Santiago Angulo, Soledad Fenoy, Carolina Hurtado, Carmen del Aguila, Graham R. Wallace PLOS ONE.2016; 11(4): e0154246. CrossRef 18S ribosomal DNA genotypes of Acanthamoeba species isolated from contact lens cases in the Philippines Windell L. Rivera, Davin Edric V. Adao Parasitology Research.2009; 105(4): 1119. CrossRef Endosymbionts of Acanthamoeba Isolated from Domestic Tap Water in Korea Seon Hee Choi, Min Kyoung Cho, Soon Cheol Ahn, Ji Eun Lee, Jong Soo Lee, Dong-Hee Kim, Ying-Hua Xuan, Yeon Chul Hong, Hyun Hee Kong, Dong Il Chung, Hak Sun Yu The Korean Journal of Parasitology.2009; 47(4): 337. CrossRef Molecular Phylogeny of Acanthamoeba Hyun Hee Kong The Korean Journal of Parasitology.2009; 47(Suppl): S21. CrossRef Detection of Acanthamoeba in Tap Water and Contact Lens Cases Using Polymerase Chain Reaction Maureen Boost, Pauline Cho, Sindy Lai, Wing-Man Sun Optometry and Vision Science.2008; 85(7): 526. CrossRef Acanthamoeba spp. and bacterial contamination in contact lens storage cases and the relationship to user profiles Claiton José Pens, Marisa da Costa, Cristina Fadanelli, Karin Caumo, MariliseBrittes Rott Parasitology Research.2008; 103(6): 1241. CrossRef Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung The Korean Journal of Parasitology.2008; 46(3): 157. CrossRef Distribution of Acanthamoeba spp. in Raw Water and Water Treatment Process Journal of the Environmental Sciences.2008; 17(10): 1121. CrossRef Molecular characterization of bacterial endosymbionts of Acanthamoeba isolates from infected corneas of Korean patients Ying-Hua Xuan, Hak Sun Yu, Hae Jin Jeong, Sung-Yong Seol, Dong-Il Chung, Hyun-Hee Kong The Korean Journal of Parasitology.2007; 45(1): 1. CrossRef Cysticidal Effect on Acanthamoeba and Toxicity on Human Keratocytes by Polyhexamethylene Biguanide and Chlorhexidine Ji-Eun Lee, Boo Sup Oum, Hee Young Choi, Hak Sun Yu, Jong Soo Lee Cornea.2007; 26(6): 736. CrossRef Acanthamoeba: Keratopathogenicity of isolates from domestic tap water in Korea Hae Jin Jeong, Sun Joo Lee, Jeong Hwan Kim, Ying Hua Xuan, Keun Hee Lee, Sang Kyun Park, Sun Hee Choi, Dong Il Chung, Hyun Hee Kong, Mee Sun Ock, Hak Sun Yu Experimental Parasitology.2007; 117(4): 357. CrossRef Factors Affecting the Epidemiology ofAcanthamoebaKeratitis Youhanna W. Ibrahim, David L. Boase, Ian A. Cree Ophthalmic Epidemiology.2007; 14(2): 53. CrossRef Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear Sun Joo Lee, Hae Jin Jeong, Ji Eun Lee, Jong Soo Lee, Ying Hua Xuan, Hyun-Hee Kong, Dong-Il Chung, Mee-Sun Ock, Hak Sun Yu The Korean Journal of Parasitology.2006; 44(4): 313. CrossRef The role of domestic tap water in Acanthamoeba contamination in contact lens storage cases in Korea Hae Jin Jeong, Hak Sun Yu The Korean Journal of Parasitology.2005; 43(2): 47. CrossRef Acanthamoeba keratitis update—incidence, molecular epidemiology and new drugs for treatment D V Seal Eye.2003; 17(8): 893. CrossRef Mitochondrial DNA Restriction Fragment Length Polymorphism (RFLP) and 18S Small-Subunit Ribosomal DNA PCR-RFLP Analyses ofAcanthamoebaIsolated from Contact Lens Storage Cases of Residents in Southwestern Korea Hyun-Hee Kong, Ji-Yeol Shin, Hak-Sun Yu, Jin Kim, Tae-Won Hahn, Young-Ho Hahn, Dong-Il Chung Journal of Clinical Microbiology.2002; 40(4): 1199. 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