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Luiz Claudio Miletti	1 Article
First Evaluation of an Outbreak of Bovine Babesiosis and Anaplasmosis in Southern Brazil Using Multiplex PCR Mariana Feltrin Canever, Luisa Lemos Vieira, Carolina Reck, Luisa Richter, Luiz Claudio Miletti Korean J Parasitol 2014;52(5):507-511. Published online October 22, 2014 DOI: https://doi.org/10.3347/kjp.2014.52.5.507 Abstract PDF Outbreaks of tick-borne disease cases in Santa Catarina, Brazil are known, but the presence of the pathogen DNA has never been determined. In this study, the first survey of Anaplasma marginale, Babesia bigemina, and Babesia bovis DNA on blood samples of 33 cattle from an outbreak in Ponte Alta Municipality, Santa Catarina, Brazil, has been carried out. A multiplex PCR detected 54.5% of animals were co-infected with 2 or 3 parasites, while 24.2% were infected with only 1 species. The most prevalent agent was B. bigemina (63.6%) followed by A. marginale (60.6%). This is the first report of tick-borne disease pathogens obtained by DNA analysis in Southern Brazil. Citations Citations to this article as recorded by Prevalence and factors associated with Babesia bigemina infection in Crioula Lageana cattle breed Mariana da Silva Casa, Julio de Mattos Vettori, Ketriane Mota de Souza, Paulo Ricardo Benetti Todeschini, Luiz Cláudio Miletti, Carla Ivane Ganz Vogel, André Luís Ferreira Lima, Joandes Henrique Fonteque Revista Brasileira de Parasitologia Veterinária.2025;[Epub] CrossRef Revisão sistemática sobre diagnóstico molecular da tristeza parasitária bovina no Brasil: ocorrência e oportunidades futuras Natália Machado Rahal, Kauê Rodriguez Martins, Josiane de Oliveira Feijó, Marco Antonio Tonus Marinho, Marcio Nunes Corrêa Semina: Ciências Agrárias.2024; 45(3): 835. CrossRef Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing Mariana da Silva Casa, Guillermo Giovambattista, Graziela Vieira Fonteque, Ellen Lara Miguel, Carla Ivane Ganz Vogel, Luiz Claudio Miletti, Shin-Nosuke Takeshima, Joandes Henrique Fonteque Frontiers in Veterinary Science.2023;[Epub] CrossRef Proportion and seasonality of blood parasites in animals in Mosul using the Veterinary Teaching Hospital Lab data Hussam M. S. Alimam, Dhiyaa A. Moosa, Eva A. Ajaj, Mohammad O. Dahl, Israa A. Al-Robaiee, Semaa F. Hasab Allah, Zahraa M. Al-Jumaa, Eman D. Hadi, Simon Clegg PLOS ONE.2022; 17(2): e0264121. CrossRef Prevalence of bovine Babesia spp., Anaplasma marginale, and their co-infections in Latin America: Systematic review-meta-analysis Gabriella Carvalho Mattos Ferreira, Maria Eugênia Andrighetto Canozzi, Vanessa Peripolli, Gabriely de Paula Moura, Javier Sánchez, Carlos Eduardo Nogueira Martins Ticks and Tick-borne Diseases.2022; 13(4): 101967. CrossRef Validation of a multiplex PCR assay to detect Babesia spp. and Anaplasma marginale in cattle in Uruguay in the absence of a gold standard test Pablo Parodi, Luis G. Corbellini, Vanessa B. Leotti, Rodolfo Rivero, Cecilia Miraballes, Franklin Riet-Correa, José M. Venzal, María T. Armúa-Fernández Journal of Veterinary Diagnostic Investigation.2021; 33(1): 73. CrossRef Low level of genetic diversity and high occurrence of vector-borne protozoa in water buffaloes in Thailand based on 18S ribosomal RNA and mitochondrial cytochrome b genes Anh H.L. Nguyen, Sonthaya Tiawsirisup, Morakot Kaewthamasorn Infection, Genetics and Evolution.2020; 82: 104304. CrossRef Molecular diagnosis and biochemical studies of tick-borne diseases (anaplasmosis and babesiosis) in Aberdeen Angus Cattle in New Valley, Egypt Nani Nasreldin, Rania M. Ewida, Hatem Hamdon, Yasser F. Elnaker Veterinary World.2020; 13(9): 1884. CrossRef Prevalence of Anaplasma marginale, Babesia bovis, and Babesia bigemina in cattle in the Campos de Lages region, Santa Catarina state, Brazil, estimated by multiplex-PCR Luisa Lemos Vieira, Mariana Feltrin Canever, Leonardo Leite Cardozo, Cristina Perito Cardoso, Marcos Edgar Herkenhoff, André Thaler Neto, Carla Ivane Ganz Vogel, Luiz Claudio Miletti Parasite Epidemiology and Control.2019; 6: e00114. CrossRef Molecular surveillance and phylogenetic traits of Babesia bigemina and Babesia bovis in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) from Colombia Jeiczon Jaimes-Dueñez, Omar Triana-Chávez, Andrés Holguín-Rocha, Alberto Tobon-Castaño, Ana M. Mejía-Jaramillo Parasites & Vectors.2018;[Epub] CrossRef Phylogenetic relationship and genotypic variability in Anaplasma marginale strains causing anaplasmosis in India Neena George, Vasundhra Bhandari, Paresh Sharma Infection, Genetics and Evolution.2017; 48: 71. CrossRef Parasitological and molecular surveys reveal high rates of infection with vector-borne pathogens and clinical anemia signs associated with infection in cattle from two important livestock areas in Colombia Jeiczon Jaimes-Dueñez, Omar Triana-Chávez, Ana M. Mejía-Jaramillo Ticks and Tick-borne Diseases.2017; 8(2): 290. CrossRef High-resolution melting PCR assay, applicable for diagnostics and screening studies, allowing detection and differentiation of several Babesia spp. infecting humans and animals Wioletta Rozej-Bielicka, Aleksander Masny, Elzbieta Golab Parasitology Research.2017; 116(10): 2671. CrossRef First molecular survey and novel genetic variants’ identification of Anaplasma marginale, A. centrale and A. bovis in cattle from Tunisia Hanène Belkahia, Mourad Ben Said, Alberto Alberti, Khaoula Abdi, Zakia Issaoui, Dorra Hattab, Mohamed Gharbi, Lilia Messadi Infection, Genetics and Evolution.2015; 34: 361. CrossRef 10,172 View 114 Download 16 Web of Science Crossref