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M. Diab	1 Article
Fasciola gigantica Fatty Acid Binding Protein (FABP) as a Prophylactic Agent against Schistosoma mansoni Infection in CD1 Mice Ibrahim Rabia Aly, M. Diab, A. M. El-Amir, M. Hendawy, S. Kadry Korean J Parasitol 2012;50(1):37-43. Published online March 6, 2012 DOI: https://doi.org/10.3347/kjp.2012.50.1.37 Abstract PDF Although schistosomicidal drugs and other control measures exist, the advent of an efficacious vaccine remains the most potentially powerful means for controlling this disease. In this study, native fatty acid binding protein (FABP) from Fasciola gigantica was purified from the adult worm's crude extract by saturation with ammonium sulphate followed by separation on DEAE-Sephadex A-50 anion exchange chromatography and gel filtration using Sephacryl HR-100, respectively. CD1 mice were immunized with the purified, native F. gigantica FABP in Freund's adjuvant and challenged subcutaneously with 120 Schistosoma mansoni cercariae. Immunization of CD1 mice with F. gigantica FABP has induced heterologous protection against S. mansoni, evidenced by the significant reduction in mean worm burden (72.3%), liver and intestinal egg counts (81.3% and 80.8%, respectively), and hepatic granuloma counts (42%). Also, it elicited mixed IgG₁/IgG_2b immune responses with predominant IgG1 isotype, suggesting that native F. gigantica FABP is mediated by a mixed Th1/Th2 response. However, it failed to induce any significant differences in the oogram pattern or in the mean granuloma diameter. This indicated that native F. gigantica FABP could be a promising vaccine candidate against S. mansoni infection. Citations Citations to this article as recorded by Investigating the Taenia solium Fatty Acid Binding Protein Superfamily for Their Immunological Outlook and Prospect for Therapeutic Targets Suraj S. Rawat, Gagandeep Singh, Amit Prasad ACS Omega.2024; 9(21): 22557. CrossRef Potential Utility of Systemic Plasma Biomarkers for Evaluation of Pediatric Schistosomiasis in Western Kenya Bartholomew N. Ondigo, Rachael E. Hamilton, Edwin O. Magomere, Isaac O. Onkanga, Pauline N. Mwinzi, Maurice R. Odiere, Lisa Ganley-Leal Frontiers in Immunology.2022;[Epub] CrossRef Effect of regulatory T cells on the efficacy of the fatty acid-binding protein vaccine against Schistosoma japonicum Chun-lian Tang, Rong-hui Zhang, Zhi-ming Liu, Huang Jin, Long He Parasitology Research.2019; 118(2): 559. CrossRef Protection against Schistosoma mansoni infection using a Fasciola hepatica-derived fatty acid binding protein from different delivery systems Belén Vicente, Julio López-Abán, Jose Rojas-Caraballo, Esther del Olmo, Pedro Fernández-Soto, Antonio Muro Parasites & Vectors.2016;[Epub] CrossRef Schistosomiasis in Egypt: A never-ending story? Ahmad A. Othman, Rasha H. Soliman Acta Tropica.2015; 148: 179. CrossRef Development of the Brazilian Anti Schistosomiasis Vaccine Based on the Recombinant Fatty Acid Binding Protein Sm14 Plus GLA-SE Adjuvant Miriam Tendler, Marilia Almeida, Andrew Simpson Frontiers in Immunology.2015;[Epub] CrossRef The transcriptome of Echinostoma caproni adults: Further characterization of the secretome and identification of new potential drug targets Gagan Garg, Dolores Bernal, Maria Trelis, Javier Forment, Javier Ortiz, M. Luz Valero, Laia Pedrola, Juan Martinez-Blanch, J. Guillermo Esteban, Shoba Ranganathan, Rafael Toledo, Antonio Marcilla Journal of Proteomics.2013; 89: 202. CrossRef Molecular characterization, functional expression, tissue localization and protective potential of a Taenia solium fatty acid-binding protein Oscar Illescas, Julio C. Carrero, Raúl J. Bobes, Ana Flisser, Gabriela Rosas, Juan P. Laclette Molecular and Biochemical Parasitology.2012; 186(2): 117. CrossRef 9,189 View 95 Download Crossref