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Mohammad Saiful slam	1 Article
Proteomic Screening of Antigenic Proteins from the Hard Tick, Haemaphysalis longicornis (Acari: Ixodidae) Young-Ha Kim, Mohammad Saiful slam, Myung-Jo You Korean J Parasitol 2015;53(1):85-93. Published online February 27, 2015 DOI: https://doi.org/10.3347/kjp.2015.53.1.85 Abstract PDF Proteomic tools allow large-scale, high-throughput analyses for the detection, identification, and functional investigation of proteome. For detection of antigens from Haemaphysalis longicornis, 1-dimensional electrophoresis (1-DE) quantitative immunoblotting technique combined with 2-dimensional electrophoresis (2-DE) immunoblotting was used for whole body proteins from unfed and partially fed female ticks. Reactivity bands and 2-DE immunoblotting were performed following 2-DE electrophoresis to identify protein spots. The proteome of the partially fed female had a larger number of lower molecular weight proteins than that of the unfed female tick. The total number of detected spots was 818 for unfed and 670 for partially fed female ticks. The 2-DE immunoblotting identified 10 antigenic spots from unfed females and 8 antigenic spots from partially fed females. Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF) of relevant spots identified calreticulin, putative secreted WC salivary protein, and a conserved hypothetical protein from the National Center for Biotechnology Information and Swiss Prot protein sequence databases. These findings indicate that most of the whole body components of these ticks are non-immunogenic. The data reported here will provide guidance in the identification of antigenic proteins to prevent infestation and diseases transmitted by H. longicornis. Citations Citations to this article as recorded by Developmental Proteomics Reveals the Dynamic Expression Profile of Global Proteins of Haemaphysalis longicornis (Parthenogenesis) Min-Xuan Liu, Xiao-Pei Xu, Fan-Ming Meng, Bing Zhang, Wei-Gang Li, Yuan-Yuan Zhang, Qiao-Ying Zen, Wen-Ge Liu Life.2025; 15(1): 59. CrossRef Haemaphysalis longicornis calreticulin is not an effective molecular tool for tick bite diagnosis and disruption of tick infestations Weiqing Zheng, Haijun Hu, Jiafu Jiang, Xiangrong Sun, Renlong Fu, Huiying Tao, Yangqing Liu, Haiying Chen, Hongmei Ma, Shengen Chen Veterinary Parasitology.2022; 309: 109775. CrossRef Trypanosoma cruzi Calreticulin: Immune Evasion, Infectivity, and Tumorigenesis Galia Ramírez-Toloza, Eduardo Sosoniuk-Roche, Carolina Valck, Lorena Aguilar-Guzmán, Viviana P. Ferreira, Arturo Ferreira Trends in Parasitology.2020; 36(4): 368. CrossRef Catalogue of stage-specific transcripts in Ixodes ricinus and their potential functions during the tick life-cycle Pavlina Vechtova, Zoltan Fussy, Radim Cegan, Jan Sterba, Jan Erhart, Vladimir Benes, Libor Grubhoffer Parasites & Vectors.2020;[Epub] CrossRef Comparative Tandem Mass Tag-Based Quantitative Proteomic Analysis of Tachaea chinensis Isopod During Parasitism Yingdong Li, Xin Li, Zhibin Han, Weibin Xu, Xiaodong Li, Qijun Chen Frontiers in Cellular and Infection Microbiology.2019;[Epub] CrossRef Applying proteomics to tick vaccine development: where are we? Margarita Villar, Anabel Marina, José de la Fuente Expert Review of Proteomics.2017; 14(3): 211. CrossRef Screening and Identification of Antigenic Proteins from the Hard Tick <i>Dermacentor silvarum</i> (Acari: Ixodidae) Tiantian Zhang, Xuejiao Cui, Jincheng Zhang, Hui Wang, Meng Wu, Hua Zeng, Yuanyuan Cao, Jingze Liu, Yonghong Hu The Korean Journal of Parasitology.2015; 53(6): 789. CrossRef 10,707 View 108 Download 7 Web of Science Crossref