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Mojtaba Saffari	1 Article
Overexpression of Ubiquitin and Amino Acid Permease Genes in Association with Antimony Resistance in Leishmania tropica Field Isolates Elham Kazemi-Rad, Mehdi Mohebali, Mohammad Bagher Khadem-Erfan, Homa Hajjaran, Ramtin Hadighi, Ali Khamesipour, Sassan Rezaie, Mojtaba Saffari, Reza Raoofian, Mansour Heidari Korean J Parasitol 2013;51(4):413-419. Published online August 30, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.4.413 Abstract PDF The mainstay therapy against leishmaniasis is still pentavalent antimonial drugs; however, the rate of antimony resistance is increasing in endemic regions such as Iran. Understanding the molecular basis of resistance to antimonials could be helpful to improve treatment strategies. This study aimed to recognize genes involved in antimony resistance of Leishmania tropica field isolates. Sensitive and resistant L. tropica parasites were isolated from anthroponotic cutaneous leishmaniasis patients and drug susceptibility of parasites to meglumine antimoniate (Glucantime®) was confirmed using in vitro assay. Then, complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) and real-time reverse transcriptase-PCR (RT-PCR) approaches were utilized on mRNAs from resistant and sensitive L. tropica isolates. We identified 2 known genes, ubiquitin implicated in protein degradation and amino acid permease (AAP3) involved in arginine uptake. Also, we identified 1 gene encoding hypothetical protein. Real-time RT-PCR revealed a significant upregulation of ubiquitin (2.54-fold), and AAP3 (2.86-fold) (P<0.05) in a resistant isolate compared to a sensitive one. Our results suggest that overexpression of ubiquitin and AAP3 could potentially implicated in natural antimony resistance. Citations Citations to this article as recorded by Comparative transcriptomics of naturally susceptible and resistant Trypanosoma cruzi strains in response to Benznidazole Carlos Ospina, Tatiana Cáceres, Stivenn Gutiérrez, Luz Helena Patiño, Luis David Sáenz-Pérez, Karen Moreno Medina, Juan Carlos Villar, Juan David Ramírez International Journal for Parasitology: Drugs and Drug Resistance.2025; 29: 100623. CrossRef Leishmania spp. genetic factors associated with cutaneous leishmaniasis antimony pentavalent drug resistance: a systematic review Raphaela Lisboa Andrade Nery, Thaline Mabel Sousa Santos, Luana Leandro Gois, Aldina Barral, Ricardo Khouri, Caroline Alves Feitosa, Luciane Amorim Santos Memórias do Instituto Oswaldo Cruz.2024;[Epub] CrossRef Transcriptomic analysis of benznidazole-resistant and susceptible Trypanosoma cruzi populations Davi Alvarenga Lima, Leilane Oliveira Gonçalves, João Luís Reis-Cunha, Paul Anderson Souza Guimarães, Jeronimo Conceição Ruiz, Daniel Barbosa Liarte, Silvane Maria Fonseca Murta Parasites & Vectors.2023;[Epub] CrossRef Quantitative proteomic analysis reveals differential modulation of crucial stage specific proteins during promastigote to amastigote differentiation in Leishmania donovani Chinmayee Bar Routaray, Vaibhavee Choudhari, Divya Prakash, Rajendra Patil, Surabhi Jagtap, Shakuntala Bai, Mahesh J. Kulkarni, Suresh V. Kuchipudi, Kalpana Pai Journal of Proteins and Proteomics.2022; 13(1): 17. CrossRef Leishmania infantum UBC1 in Metacyclic Promastigotes from Phlebotomus perniciosus, a Vaccine Candidate for Zoonotic Visceral Leishmaniasis Jaime Larraga, Pedro Alcolea, Ana Alonso, Luis Martins, Inmaculada Moreno, Mercedes Domínguez, Vicente Larraga Vaccines.2022; 10(2): 231. CrossRef Global distribution of treatment resistance gene markers for leishmaniasis Samira Salari, Mehdi Bamorovat, Iraj Sharifi, Pooya Ghasemi Nejad Almani Journal of Clinical Laboratory Analysis.2022;[Epub] CrossRef Elucidation of the Flavor Aspects and Flavor-Associated Genomic Regions in Bottle Gourd (Lagenaria siceraria) by Metabolomic Analysis and QTL-seq Ying Wang, Yanwei Li, Xiaohua Wu, Xinyi Wu, Zishan Feng, Jian Wang, Baogen Wang, Zhongfu Lu, Guojing Li Foods.2022; 11(16): 2450. CrossRef Effect of the variation in the extracellular concentration of l-arginine in the physiology of Leishmania (Viannia) braziliensis and its susceptibility to some antileishmanial drugs Manuela Giraldo, Yulieth A. Upegui, Jorge L. Higuita-Castro, Luis A. Gonzalez, Sneider Gutierrez, Sergio A. Pulido, Sara M. Robledo Experimental Parasitology.2022; 242: 108395. CrossRef HAS 1: A natural product from soil-isolated Streptomyces species with potent activity against cutaneous leishmaniasis caused by Leishmania tropica Bassel Awada, Maguy Hamie, Rana El Hajj, Ghada Derbaj, Rania Najm, Perla Makhoul, Dima Hajj Ali, Antoine G. Abou Fayad, Hiba El Hajj Frontiers in Pharmacology.2022;[Epub] CrossRef Metabolite Biomarkers of Leishmania Antimony Resistance Sneider Alexander Gutierrez Guarnizo, Zemfira N. Karamysheva, Elkin Galeano, Carlos E. Muskus Cells.2021; 10(5): 1063. CrossRef Voriconazole resistance genes in Aspergillus flavus clinical isolates F. Zaini, E. Lotfali, A. Fattahi, E. Siddig, S. Farahyar, E. Kouhsari, M. Saffari Journal de Mycologie Médicale.2020; 30(2): 100953. CrossRef Comparative transcriptomic analysis of antimony resistant and susceptible Leishmania infantum lines Juvana Moreira Andrade, Leilane Oliveira Gonçalves, Daniel Barbosa Liarte, Davi Alvarenga Lima, Frederico Gonçalves Guimarães, Daniela de Melo Resende, Ana Maria Murta Santi, Luciana Marcia de Oliveira, João Paulo Linhares Velloso, Renato Guimarães Delfin Parasites & Vectors.2020;[Epub] CrossRef An overview of leishmanization experience: A successful control measure and a tool to evaluate candidate vaccines Mehdi Mohebali, Abolhassan Nadim, Ali Khamesipour Acta Tropica.2019; 200: 105173. CrossRef Comparison of Cysteine Protease B Gene Expression between Clinical Isolates of Leishmania tropica, Leishmania major and Leishmania infantum Elham Kazemirad, Hossien Reisi Nafchi, Alireza Latifi, Reza Raoofian, Mehdi Mohebali, Homa Hajjaran Journal of Medical Microbiology and Infectious Diseases.2019; 7(3): 72. CrossRef Use of AFLP for the study of eukaryotic pathogens affecting humans Carlos M. Restrepo, Alejandro Llanes, Ricardo Lleonart Infection, Genetics and Evolution.2018; 63: 360. CrossRef Expression analysis of viscerotropic leishmaniasis gene in Leishmania species by real-time RT-PCR Hossein Reisi Nafchi, Elham Kazemi-Rad, Mehdi Mohebali, Reza Raoofian, Niloofar Bavarsad Ahmadpour, Mohammad Ali Oshaghi, Homa Hajjaran Acta Parasitologica.2016;[Epub] CrossRef Expression analysis of activated protein kinase C gene (LACK1) in antimony sensitive and resistant Leishmania tropica clinical isolates using real‐time RT‐PCR Homa Hajjaran, Elham Kazemi‐Rad, Mehdi Mohebali, Mohammad A. Oshaghi, Mohammad B. Khadem‐Erfan, Elham Hajaliloo, Hossein Reisi Nafchi, Reza Raoofian International Journal of Dermatology.2016; 55(9): 1020. CrossRef Visceral Leishmaniasis without Fever in an 11-Month-Old Infant: a Rare Clinical Feature of Kala-azar Shirin Sayyahfar, Shahla Ansari, Mehdi Mohebali, Babak Behnam The Korean Journal of Parasitology.2014; 52(2): 189. CrossRef 8,989 View 90 Download Crossref