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Myeong-Heon Shin	3 Articles
Excretory-secretory product of newly excysted metacercariae of Paragonimus westermani directly induces eosinophil apoptosis Myeong-Heon Shin Korean J Parasitol 2000;38(1):17-23. Published online March 31, 2000 DOI: https://doi.org/10.3347/kjp.2000.38.1.17 Abstract PDF Eosinophils are important effector cells in host defense against parasites. Excretory-secretory product (ESP) produced by helminthic worms plays important roles in the uptake of nutrients, migration in the host tissue, and in immune modulation. However, little is known about the ability of the ESP to directly trigger eosinophil apoptosis. This study investigated whether the ESP of newly excysted metacercariae of Paragonimus westermani could induce apoptosis in human eosinophils. Apoptosis was assayed by staining the cells with FITC-annexin V, and the cells were analyzed by flow cytometry. It was found that the ESP of newly excysted metacercariae of P. westermani induced a direct time- and concentration-dependent increase in the rate of constitutive apoptosis in mature human eosinophils. Eosinophil apoptosis was first apparent 3 hr after treatment with the ESP and continued to increase after 6 hr of incubation with respect to the cells cultured in the absence of the ESP. While only 2.8% of the eosinophils incubated in the medium for 3 hr were apoptotic, 7.6%, 10.9% and 22.6% of the eosinophils treated with 10, 30 and 100 ?g/ml ESP were apoptotic, respectively. This result suggests that the ESP of newly excysted metacercariae of P. westermani directly induce eosinophil apoptosis, which may be important for the survival of the parasites and the reduction of eosinophilic inflammation in vivo. Citations Citations to this article as recorded by Gastric lesions in dolphins stranded along the Eastern Adriatic coast J Hrabar, I Bočina, A Gudan Kurilj, M Đuras, I Mladineo Diseases of Aquatic Organisms.2017; 125(2): 125. CrossRef Fasciola hepatica induces eosinophil apoptosis in the migratory and biliary stages of infection in sheep A. Escamilla, M.J. Bautista, R. Zafra, I.L. Pacheco, M.T. Ruiz, S. Martínez-Cruz, A. Méndez, A. Martínez-Moreno, V. Molina-Hernández, J. Pérez Veterinary Parasitology.2016; 216: 84. CrossRef Fasciola hepatica products induce apoptosis of peritoneal macrophages Lorena Guasconi, Marianela C. Serradell, Diana T. Masih Veterinary Immunology and Immunopathology.2012; 148(3-4): 359. CrossRef Staphylococcus aureus Induces Eosinophil Cell Death Mediated by α-hemolysin Lynne R. Prince, Kirstie J. Graham, John Connolly, Sadia Anwar, Robert Ridley, Ian Sabroe, Simon J. Foster, Moira K. B. Whyte, Malcolm James Horsburgh PLoS ONE.2012; 7(2): e31506. CrossRef A 27 kDa Cysteine Protease Secreted by Newly Excysted Paragonimus westermani Metacercariae Induces Superoxide Anion Production and Degranulation of Human Eosinophils Young-Bae Chung, Hirohito Kita, Myeong Heon Shin The Korean Journal of Parasitology.2008; 46(2): 95. CrossRef Excretory-secretory products from Fasciola hepatica induce eosinophil apoptosis by a caspase-dependent mechanism Marianela C. Serradell, Lorena Guasconi, Laura Cervi, Laura S. Chiapello, Diana T. Masih Veterinary Immunology and Immunopathology.2007; 117(3-4): 197. CrossRef *Excretory-Secretory Products Produced by Paragonimus westermani* Differentially Regulate the Nitric Oxide Production and Viability of Microglial Cells** Youngnam Jin, Jae-Chul Lee, In Young Choi, Eun A. Kim, Myeong Heon Shin, Won-Ki Kim International Archives of Allergy and Immunology.2006; 139(1): 16. CrossRef Cysteine Protease Secreted byParagonimus westermaniAttenuates Effector Functions of Human Eosinophils Stimulated with Immunoglobulin G W. A. Petri, Myeong Heon Shin, Hirohito Kita, Hae Young Park, Ju Young Seoh Infection and Immunity.2001; 69(3): 1599. CrossRef Proteolytic activity of cysteine protease in excretory–secretory product of Paragonimus westermani newly excysted metacercariae pivotally regulates IL‐8 production of human eosinophils Myeong Heon Shin, Soo Young Lee Parasite Immunology.2000; 22(10): 529. CrossRef 8,534 View 71 Download Crossref Infection status of Paragonimus westermani metacercariae in crayfish (Cambaroides similis) collected from Bogildo (Islet), Wando-gun, Chollanam-do, Korea Myeong-Heon Shin, Duk-Young Min Korean J Parasitol 1999;37(1):55-57. Published online March 31, 1999 DOI: https://doi.org/10.3347/kjp.1999.37.1.55 Abstract PDF During the period from October 1996 to November 1998, the infection status of Paragonimus westermani metacercariae in freshwater crayfish (Cambaroides similis) collected from Bogildo (islet), Wando-gun, Chollanam-do, which is known for an endemic area of P. westermani in Korea, were examined. The average infection rate of Paragonimus metacercariae in crayfish was 88.6%, and mean number of metacercariae per infected crayfish was 30.2. This metacercarial density was the highest in the group of weight in 7.1-9.0 g. These results suggest that the natural life cycle of P. westermani is still well-preserved in Bogildo. Citations Citations to this article as recorded by Recent Incidence of Paragonimus westermani Metacercariae in Freshwater Crayfish, Cambaroides similis, from Two Enzootic Sites in Jeollanam-do, Korea Jin-Ho Song, Fuhong Dai, Xuelian Bai, Tae-Im Kim, Hyun-Jong Yang, Tong-Soo Kim, Shin-Hyung Cho, Sung-Jong Hong The Korean Journal of Parasitology.2017; 55(3): 347. CrossRef Northeast India Helminth Parasite Information Database (NEIHPID): Knowledge Base for Helminth Parasites Devendra Kumar Biswal, Manish Debnath, Graciously Kharumnuid, Welfrank Thongnibah, Veena Tandon, Govindhaswamy Umapathy PLOS ONE.2016; 11(6): e0157459. CrossRef Diseases of crayfish: A review Matt Longshaw Journal of Invertebrate Pathology.2011; 106(1): 54. CrossRef Infection Status of Freshwater Crabs and Crayfish with Metacercariae of Paragonimus westermani in Korea Eun-Min Kim, Jae-Lip Kim, Sung-Il Choi, Soon-Hyung Lee, Sung-Tae Hong The Korean Journal of Parasitology.2009; 47(4): 425. CrossRef North American Paragonimiasis (Caused byParagonimus kellicotti) in the Context of Global Paragonimiasis Gary W. Procop Clinical Microbiology Reviews.2009; 22(3): 415. CrossRef A 27 kDa Cysteine Protease Secreted by Newly Excysted Paragonimus westermani Metacercariae Induces Superoxide Anion Production and Degranulation of Human Eosinophils Young-Bae Chung, Hirohito Kita, Myeong Heon Shin The Korean Journal of Parasitology.2008; 46(2): 95. CrossRef Cysteine Protease Secreted byParagonimus westermaniAttenuates Effector Functions of Human Eosinophils Stimulated with Immunoglobulin G W. A. Petri, Myeong Heon Shin, Hirohito Kita, Hae Young Park, Ju Young Seoh Infection and Immunity.2001; 69(3): 1599. CrossRef 8,074 View 91 Download Crossref Genetic variance of Trichomonas vaginalis isolates by Southern hybridization Jae-Sook Ryu, Duk-Young Min, Myeong-Heon Shin, Youl-Hee Cho Korean J Parasitol 1998;36(3):207-211. Published online September 20, 1998 DOI: https://doi.org/10.3347/kjp.1998.36.3.207 Abstract PDF In the present study, genomic DNAs were purified from Korean isolates (KT8, KT6, KT-Kim and KT-Lee) and foreign strains (CDC85, IR78 and NYH 286) of Trichomonas vaginalis, and hybridized with a probe based on the repetitive sequence cloned from T. vaginalis to observe the genetic differences. By Southern hybridization, all isolates of T. vaginalis except the NYH286 strain had 11 bands. Therefore all isolates examined were distinguishable into 3 groups according to their banding patterns; i) KT8, KT6 and KT-Kim isolates had 11 identical bands such as 1 kb, 1.2 kb, 1.6 kb, 1.9 kb, 2.3 kb, 2.7 kb, 3.2 kb, 3.4 kb, 3.8 kb, 4.9 kb and 6.0 kb. ii) The metronidazole-resistant IR78 strain had the same bands as KT-Lee isolate at bands of 1 kb, 1.2 kb, 1.6 kb, 1.8 kb, 2.1 kb, 2.5 kb, 2.7 kb, 2.9 kb, 3.4 kb, 5.0 kb and 6.0 kb. Bands of CDC85, metronidazole-resistant strain, were similar to those of IR78 and KT-Lee, except that 3.2 kb replaced 2.9 kb. iii) NYH286 particularly had 12 bands and band patterns were similar to IR78 with a few exceptions as follows: i) 6.2 kb in place of 6.0 kb, ii) 2.0 kb and 2.2 kb instead of 2.1 kb. Through the results obtained, genetic variance of T. vaginalis isolates was demonstrated by Southern hybridization. Citations Citations to this article as recorded by Population structure and genetic diversity of Trichomonas vaginalis clinical isolates in Australia and Ghana Daniel S. Squire, Alan J. Lymbery, Jennifer Walters, Frances Brigg, Andrea Paparini, R.C. Andrew Thompson Infection, Genetics and Evolution.2020; 82: 104318. CrossRef Genotypic Variation in Trichomonas vaginalis Detected in South African Pregnant Women Rennisha Chetty, Nonkululeko Mabaso, Nathlee Abbai Infectious Diseases in Obstetrics and Gynecology.2020; 2020: 1. CrossRef Genetic Diversity of Trichomonas Vaginalis Clinical Isolates According to Restriction Fragment Length Polymorphism Analysis of the 60-kDa Proteinase Gene Hilda Hernández, Jorge Fraga, Ricardo Marcet, Annia Alba, Mabel Figueredo, Yenisey Alfonso, Lázara Rojas, Jorge Sarracent Acta Parasitologica.2019; 64(2): 300. CrossRef Genetic diversity inTrichomonas vaginalis John C Meade, Jane M Carlton Sexually Transmitted Infections.2013; 89(6): 444. CrossRef Genetic Characterization of Trichomonas vaginalis Isolates by Use of Multilocus Sequence Typing Denise C. Cornelius, D. Ashley Robinson, Christina A. Muzny, Leandro A. Mena, David M. Aanensen, William B. Lushbaugh, John C. Meade Journal of Clinical Microbiology.2012; 50(10): 3293. CrossRef Double-stranded RNA viral infection of Trichomonas vaginalis and correlation with genetic polymorphism of isolates Jorge Fraga, Lazara Rojas, Idalia Sariego, Ayme Fernández-Calienes Experimental Parasitology.2011; 127(2): 593. CrossRef Genetic variability between Trichomonas vaginalis isolates and correlation with clinical presentation Lazara Rojas, Jorge Fraga, Idalia Sariego Infection, Genetics and Evolution.2004; 4(1): 53. CrossRef 8,746 View 58 Download Crossref