Human schistosomiasis caused by Schistosoma japonicum and Schistosoma mekongi is a chronic and debilitating helminthic disease still prevalent in several countries of Asia. Due to morphological similarities of cercariae and eggs of these 2 species, microscopic differentiation is difficult. High resolution melting (HRM) real-time PCR is developed as an alternative tool for the detection and differentiation of these 2 species. A primer pair was designed for targeting the 18S ribosomal RNA gene to generate PCR products of 156 base pairs for both species. The melting points of S. japonicum and S. mekongi PCR products were 84.5±0.07℃ and 85.7±0.07℃, respectively. The method permits amplification from a single cercaria or an egg. The HRM real-time PCR is a rapid and simple tool for differentiation of S. japonicum and S. mekongi in the intermediate and final hosts.
Citations
Citations to this article as recorded by
Current advances in serological and molecular diagnosis of Schistosoma mekongi infection Adrian Miki C. Macalanda, Atcharaphan Wanlop, Kevin Austin L. Ona, Eloiza May S. Galon, Virak Khieu, Somphou Sayasone, Aya Yajima, Jose Ma. M. Angeles, Shin-ichiro Kawazu Tropical Medicine and Health.2024;[Epub] CrossRef
Molecular Techniques as Alternatives of Diagnostic Tools in China as Schistosomiasis Moving towards Elimination Chao Lv, Wangping Deng, Liping Wang, Zhiqiang Qin, Xiaonong Zhou, Jing Xu Pathogens.2022; 11(3): 287. CrossRef
Development of a novel real-time polymerase chain reaction assay for the sensitive detection of Schistosoma japonicum in human stool Sara Halili, Jessica R. Grant, Nils Pilotte, Catherine A. Gordon, Steven A. Williams, Cinzia Cantacessi PLOS Neglected Tropical Diseases.2021; 15(10): e0009877. CrossRef
Establishment and application of a novel fluorescence-based analytical method for the rapid detection of viable bacteria in different samples Qiuyue Yin, Maiqian Nie, Zhenjun Diwu, Yuting Zhang, Lei Wang, Dandan Yin, Liancheng Li Analytical Methods.2020; 12(31): 3933. CrossRef
Molecular characterization and functional analysis of the Schistosoma mekongi Ca2+-dependent cysteine protease (calpain) Salisa Chaimon, Yanin Limpanont, Onrapak Reamtong, Sumate Ampawong, Orawan Phuphisut, Phiraphol Chusongsang, Jiraporn Ruangsittichai, Usa Boonyuen, Dorn Watthanakulpanich, Anthony J. O’Donoghue, Conor R. Caffrey, Poom Adisakwattana Parasites & Vectors.2019;[Epub] CrossRef
Rapid detection and identification of four major Schistosoma species by high-resolution melt (HRM) analysis Juan Li, Guang-Hui Zhao, RuiQing Lin, David Blair, Hiromu Sugiyama, Xing-Quan Zhu Parasitology Research.2015; 114(11): 4225. CrossRef
Detection of Schistosoma mansoni and Schistosoma haematobium by Real-Time PCR with High Resolution Melting Analysis Hany Sady, Hesham Al-Mekhlafi, Romano Ngui, Wahib Atroosh, Ahmed Al-Delaimy, Nabil Nasr, Salwa Dawaki, Awatif Abdulsalam, Init Ithoi, Yvonne Lim, Kek Chua, Johari Surin International Journal of Molecular Sciences.2015; 16(7): 16085. CrossRef
Bacterial detection: From microscope to smartphone Subash C.B. Gopinath, Thean-Hock Tang, Yeng Chen, Marimuthu Citartan, Thangavel Lakshmipriya Biosensors and Bioelectronics.2014; 60: 332. CrossRef
