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Sang-Hwan Oh	1 Article
Monoclonal antibodies to recombinant Der p 2, a major house dust mite allergen: specificity, epitope analysis and development of two-site capture ELISA Tai-Soon Yong, Sang-Mi Lee, Gab-Man Park, In-Yong Lee, Han-Il Ree, Kyung-Sup Kim, Sang-Hwan Oh, Jung-Won Park, Chein-Soo Hong Korean J Parasitol 1999;37(3):163-169. Published online September 20, 1999 DOI: https://doi.org/10.3347/kjp.1999.37.3.163 Abstract PDF House dust mite allergens have been well established as sensitizing agents that are important in the induction of allergic diseases. In order to analyze epitopes of the allergen and to develop a quantitative method of the allergen exposure, monoclonal antibodies against a recombinant Der p 2 (rDer p 2), one of the major allergens of Dermatophagoides pteronyssinus, were produced. Four monoclonal antibodies produced were species-specific and did not cross-react to the D. farinae crude extract. Two of the monoclonal antibodies were found to be IgG1 and the others were IgM. For the analysis of epitopes, a Der p 2 cDNA encoding 126 amino acids (aa) was dissected into three fragments with several overlapping peptides, A (aa residues 1-49), B (44-93), and C fragment (84-126). Three monoclonal antibodies showed reactivities to the recombinant B fragment and to the full-length rDer p 2, but one monoclonal antibody reacted only with the full-length rDer p 2. Two-site capture ELISA was developed using two different monoclonal antibodies for quantitating Der p 2 in house dust. The sensitivity limit was 4 ng/ml with rDer p 2 and 8 ?g/ml with the D. pteronyssinus crude extract. The result suggested that the assay using monoclonal antibodies against rDer p 2 could be useful for the environmental studies and for the standardization of mite allergen extracts. Citations Citations to this article as recorded by Determination of immunogenic epitopes in major house dust mite allergen, Der p 2, via nanoallergens Jenna Sjoerdsma, Franklin Mejia, Basar Bilgicer Annals of Allergy, Asthma & Immunology.2022; 129(2): 231. CrossRef Mapping Mimotopes for House Dust Mite Allergen Der f 7 Using a Specific Monoclonal Antibody Jianglong Peng, Hao Yin, Ying Zhou, Haoyuan Jia, Yubao Cui Protein & Peptide Letters.2019; 26(3): 184. CrossRef Expression and purification of a major allergen, Pla a 1, from Platanus acerifolia pollen and the preparation of its monoclonal antibody Wei-Wei Ni, Wen Huang, De-Qin Wu, Yan-Jun Zhou, Chun-Mei Ji, Meng-Da Cao, Miao Guo, Jin-Lu Sun, Ji-Fu Wei Molecular Medicine Reports.2017; 16(3): 2887. CrossRef Review on Ecology of House Dust Mites in Korea and Suggestion of a Standard Survey Method Tai-Soon Yong, Kyoung-Yong Jeong Pediatric Allergy and Respiratory Disease.2011; 21(1): 4. CrossRef Monoclonal antibodies against recombinant Der f 3 reveal localization of Der f 3 in the gut and faecal pellets of Dermatophagoides farinae Zheng-ke Zhan, Kun-mei Ji, Xiao-yu Liu, Zhi-gang Liu, Meng Li, Jia-jie Chen, Jia-na Li, Shi Qiu Experimental and Applied Acarology.2010; 52(1): 63. CrossRef Household Arthropod Allergens in Korea Tai-Soon Yong, Kyoung Yong Jeong The Korean Journal of Parasitology.2009; 47(Suppl): S143. CrossRef Mimotopes identify conformational B-cell epitopes on the two major house dust mite allergens Der p 1 and Der p 2 Krisztina Szalai, Jan Fuhrmann, Tea Pavkov, Michael Scheidl, Julia Wallmann, Kira H. Brämswig, Susanne Vrtala, Otto Scheiner, Walter Keller, Jean-Marie Saint-Remy, Dirk Neumann, Isabella Pali-Schöll, Erika Jensen-Jarolim Molecular Immunology.2008; 45(5): 1308. CrossRef Monoclonal antibodies to recombinant Der f 2 and development of a two‐site ELISA sensitive to major Der f 2 isoallergen in Korea K.Y. Jeong, H.S. Jin, S.H. Oh, C.‐S. Hong, I.‐Y. Lee, H.‐I. Ree, T.‐S. Yong Allergy.2002; 57(1): 29. CrossRef Monoclonal antibodies to recombinant Der f 2 and development of a two-site ELISA sensitive to major Der f 2 isoallergen in Korea K.Y. Jeong, H.S. Jin, S.H. Oh, C.-S. Hong, I.-Y. Lee, H.-I. Ree, T.-S. Yong Allergy.2002; 57(1): 29. CrossRef Localization of Der f 2 in the gut and fecal pellets of Dermatophagoides farinae K. Y. Jeong, I.‐Y. Lee, H.‐I. Ree, C.‐S. Hong, T.‐S. Yong Allergy.2002; 57(8): 729. CrossRef Localization of a major allergen, Der p 2, in the gut and faecal pellets of Dermatophagoides pteronyssinus Park, Lee, Lee, Ree, Kim, Hong, Yong Clinical & Experimental Allergy.2000; 30(9): 1293. CrossRef 9,612 View 77 Download Crossref