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Sehra Kim	3 Articles
Nucleolar translocalization of GRA10 of Toxoplasma gondii transfectionally expressed in HeLa cells Hye-Jin Ahn, Sehra Kim, Ho-Woo Nam Korean J Parasitol 2007;45(3):165-174. Published online September 20, 2007 DOI: https://doi.org/10.3347/kjp.2007.45.3.165 Abstract PDF Toxoplasma gondii GRA10 expressed as a GFP-GRA10 fusion protein in HeLa cells moved to the nucleoli within the nucleus rapidly and entirely. GRA10 was concentrated specifically in the dense fibrillar component of the nucleolus morphologically by the overlap of GFP-GRA10 transfection image with IFA images by monoclonal antibodies against GRA10 (Tg378), B23 (nucleophosmin) and C23 (nucleolin). The nucleolar translocalization of GRA10 was caused by a putative nucleolar localizing sequence (NoLS) of GRA10. Interaction of GRA10 with TATA-binding protein associated factor 1B (TAF1B) in the yeast two-hybrid technique was confirmed by GST pull-down assay and immunoprecipitation assay. GRA10 and TAF1B were also co-localized in the nucleolus after co-transfection. The nucleolar condensation of GRA10 was affected by actinomycin D. Expressed GFP-GRA10 was evenly distributed over the nucleoplasm and the nucleolar locations remained as hollows in the nucleoplasm under a low dose of actinomycin D. Nucleolar localizing and interacting of GRA10 with TAF1B suggested the participation of GRA10 in rRNA synthesis of host cells to favor the parasitism of T. gondii. Citations Citations to this article as recorded by Intracellular life of protozoan Toxoplasma gondii: Parasitophorous vacuole establishment and survival strategies JULIANA A. PORTES, ROSSIANE C. VOMMARO, LUCIO AYRES CALDAS, ERICA S. MARTINS-DUARTE BIOCELL.2023; 47(4): 929. CrossRef An in silico pipeline to filter the Toxoplasma gondii proteome for proteins that could traffic to the host cell nucleus and influence host cell epigenetic regulation Genevieve Syn, Jenefer M Blackwell, Sarra E Jamieson, Richard W Francis Memórias do Instituto Oswaldo Cruz.2018;[Epub] CrossRef A novel dense granule protein NcGRA23 in Neospora caninum Weirong Wang, Pengtao Gong, Pu Wang, Jingquan Dong, Xiaocen Wang, Zhengtao Yang, Jianhua Li, Xichen Zhang Acta Biochimica et Biophysica Sinica.2018; 50(7): 727. CrossRef How pathogens use linear motifs to perturb host cell networks Allegra Via, Bora Uyar, Christine Brun, Andreas Zanzoni Trends in Biochemical Sciences.2015; 40(1): 36. CrossRef GRA 14, a novel dense granule protein from Neospora caninum Gongzhen Liu, Xia Cui, Pan Hao, Daoyu Yang, Jing Liu, Qun Liu Acta Biochimica et Biophysica Sinica.2013; 45(7): 607. CrossRef Nucleolar scaffold protein, WDR46, determines the granular compartmental localization of nucleolin and DDX21 Yuya Hirai, Emilie Louvet, Toshiyuki Oda, Masahiro Kumeta, Yuzo Watanabe, Tsuneyoshi Horigome, Kunio Takeyasu Genes to Cells.2013; 18(9): 780. CrossRef A Genome-Wide siRNA Screen to Identify Host Factors Necessary for Growth of the Parasite Toxoplasma gondii Lindsey A. Moser, Angela M. Pollard, Laura J. Knoll, Mohamed Ali Hakimi PLoS ONE.2013; 8(6): e68129. CrossRef GRA Proteins of Toxoplasma gondii: Maintenance of Host-Parasite Interactions across the Parasitophorous Vacuolar Membrane Ho-Woo Nam The Korean Journal of Parasitology.2009; 47(Suppl): S29. CrossRef Neurological and behavioral abnormalities, ventricular dilatation, altered cellular functions, inflammation, and neuronal injury in brains of mice due to common, persistent, parasitic infection Gretchen Hermes, James W Ajioka, Krystyna A Kelly, Ernest Mui, Fiona Roberts, Kristen Kasza, Thomas Mayr, Michael J Kirisits, Robert Wollmann, David JP Ferguson, Craig W Roberts, Jong-Hee Hwang, Toria Trendler, Richard P Kennan, Yasuhiro Suzuki, Catherine Journal of Neuroinflammation.2008;[Epub] CrossRef 9,291 View 67 Download Crossref Interactions between secreted GRA proteins and host cell proteins across the parasitophorous vacuolar membrane in the parasitism of Toxoplasma gondii Hye-Jin Ahn, Sehra Kim, Hee-Eun Kim, Ho-Woo Nam Korean J Parasitol 2006;44(4):303-312. Published online December 20, 2006 DOI: https://doi.org/10.3347/kjp.2006.44.4.303 Abstract PDF Interactions between GRA proteins of dense granules in Toxoplasma gondii and host cell proteins were analyzed by yeast two-hybrid technique. The cMyc-GRA fusion proteins expressed from pGBKT7 plasmid in Y187 yeast were bound to host cell proteins from pGADT7-Rec-HeLa cDNA library transformed to AH109 yeast by mating method. By the selection procedures, a total of 939 colonies of the SD/-AHLT culture, 348 colonies of the X-α-gal positive and PCR, 157 colonies of the X-β-gal assay were chosen for sequencing the cDNA and finally 90 colonies containing ORF were selected to analyze the interactions. GRA proteins interacted with a variety of host cell proteins such as enzymes, structural and functional proteins of organellar proteins of broad spectrum. Several specific bindings of each GRA protein to host proteins were discussed presumptively the role of GRA proteins after secreting into the parasitophorous vacuoles (PV) and the PV membrane in the parasitism of this parasite. Citations Citations to this article as recorded by Toxoplasma–host endoplasmic reticulum interaction: How T. gondii activates unfolded protein response and modulates immune response Obed Cudjoe, Roger Afful, Tonny Abraham Hagan Current Research in Microbial Sciences.2024; 6: 100223. CrossRef Preparation and Preliminary Application of Epitope Peptide-Based Antibody against Toxoplasma gondii GRA3 Ru Wang, Minmin Wu, Haijian Cai, Ran An, Ying Chen, Jie Wang, Nan Zhou, Jian Du Tropical Medicine and Infectious Disease.2023; 8(3): 143. CrossRef Disruption of Dense Granular Protein 2 (GRA2) Decreases the Virulence of Neospora caninum Jingquan Dong, Nan Zhang, Panpan Zhao, Jianhua Li, Lili Cao, Xiaocen Wang, Xin Li, Ju Yang, Xichen Zhang, Pengtao Gong Frontiers in Veterinary Science.2021;[Epub] CrossRef Interorganellar communication and membrane contact sites in protozoan parasites Herbert J. Santos, Tomoyoshi Nozaki Parasitology International.2021; 83: 102372. CrossRef Descriptive Comparison of ELISAs for the Detection of Toxoplasma gondii Antibodies in Animals: A Systematic Review K. L. D. Tharaka D. Liyanage, Anke Wiethoelter, Jasmin Hufschmid, Abdul Jabbar Pathogens.2021; 10(5): 605. CrossRef Toxoplasma gondii GRA9 Regulates the Activation of NLRP3 Inflammasome to Exert Anti-Septic Effects in Mice Jae-Sung Kim, Seok-Jun Mun, Euni Cho, Donggyu Kim, Wooic Son, Hye-In Jeon, Hyo Keun Kim, Kiseok Jang, Chul-Su Yang International Journal of Molecular Sciences.2020; 21(22): 8437. CrossRef Identification of two novel host proteins interacting with Toxoplasma gondii 14-3-3 protein by yeast two-hybrid system Fa-Cai Li, Qing Liu, Hany M. Elsheikha, Wen-Bin Yang, Jun-Ling Hou, Xing-Quan Zhu Parasitology Research.2018; 117(4): 1291. CrossRef Toxoplasma gondii GRA8 induces ATP5A1–SIRT3-mediated mitochondrial metabolic resuscitation: a potential therapy for sepsis Ye-Ram Kim, Jae-Sung Kim, Jin-Seung Yun, Sojin Kim, Sun Young Kim, Kiseok Jang, Chul-Su Yang Experimental & Molecular Medicine.2018; 50(3): e464. CrossRef Secretory organelle trafficking in Toxoplasma gondii: A long story for a short travel Kannan Venugopal, Sabrina Marion International Journal of Medical Microbiology.2018; 308(7): 751. CrossRef Measurement of binding strength between prey proteins interacting with Toxoplasma gondii SAG1 and SAG2 using isothermal titration calorimetry (ITC) Meng-Yee Lai, Yee-Ling Lau Acta Parasitologica.2018; 63(1): 106. CrossRef Toxoplasma gondii GRA7-Targeted ASC and PLD1 Promote Antibacterial Host Defense via PKCα Hyun-Jung Koh, Ye-Ram Kim, Jae-Sung Kim, Jin-Seung Yun, Kiseok Jang, Chul-Su Yang, Sarah M. Fortune PLOS Pathogens.2017; 13(1): e1006126. CrossRef The Toxoplasma Parasitophorous Vacuole: An Evolving Host–Parasite Frontier Barbara Clough, Eva-Maria Frickel Trends in Parasitology.2017; 33(6): 473. CrossRef Perturbed human sub-networks by Fusobacterium nucleatum candidate virulence proteins Andreas Zanzoni, Lionel Spinelli, Shérazade Braham, Christine Brun Microbiome.2017;[Epub] CrossRef Screening and identification of host proteins interacting with Toxoplasma gondii SAG2 by yeast two-hybrid assay Meng-Yee Lai, Yee-Ling Lau Parasites & Vectors.2017;[Epub] CrossRef Transcriptional changes of mouse splenocyte organelle components following acute infection with Toxoplasma gondii Jun-Jun He, Jun Ma, Fa-Cai Li, Hui-Qun Song, Min-Jun Xu, Xing-Quan Zhu Experimental Parasitology.2016; 167: 7. CrossRef Toxoplasma gondii GRA7-Induced TRAF6 Activation Contributes to Host Protective Immunity Chul-Su Yang, Jae-Min Yuk, Young-Ha Lee, Eun-Kyeong Jo, J. H. Adams Infection and Immunity.2016; 84(1): 339. CrossRef Evaluation of an indirect ELISA using recombinant granule antigen GRA1, GRA7 and soluble antigens for serodiagnosis of Toxoplasma gondii infection in chickens Xuefei Sun, Zedong Wang, Jiping Li, Feng Wei, Quan Liu Research in Veterinary Science.2015; 100: 161. CrossRef Comparative mapping of host–pathogen protein–protein interactions Priya S Shah, Jason A Wojcechowskyj, Manon Eckhardt, Nevan J Krogan Current Opinion in Microbiology.2015; 27: 62. CrossRef Selective and strain-specific NFAT4 activation by the Toxoplasma gondii polymorphic dense granule protein GRA6 Ji Su Ma, Miwa Sasai, Jun Ohshima, Youngae Lee, Hironori Bando, Kiyoshi Takeda, Masahiro Yamamoto Journal of Experimental Medicine.2014; 211(10): 2013. CrossRef Recombinant Dense Granular Protein (GRA5) for Detection of Human Toxoplasmosis by Western Blot Xiao Teng Ching, Yee Ling Lau, Mun Yik Fong, Veeranoot Nissapatorn, Hemah Andiappan BioMed Research International.2014; 2014: 1. CrossRef A novel dense granule protein, GRA22, is involved in regulating parasite egress in Toxoplasma gondii Tadashi Okada, Dini Marmansari, Zeng-mei Li, Altanchimeg Adilbish, Shishenkov Canko, Akio Ueno, Haruhi Shono, Hidefumi Furuoka, Makoto Igarashi Molecular and Biochemical Parasitology.2013; 189(1-2): 5. CrossRef GRA 14, a novel dense granule protein from Neospora caninum Gongzhen Liu, Xia Cui, Pan Hao, Daoyu Yang, Jing Liu, Qun Liu Acta Biochimica et Biophysica Sinica.2013; 45(7): 607. CrossRef Transcriptomic Analysis of Toxoplasma Development Reveals Many Novel Functions and Structures Specific to Sporozoites and Oocysts Heather M. Fritz, Kerry R. Buchholz, Xiucui Chen, Blythe Durbin-Johnson, David M. Rocke, Patricia A. Conrad, John C. Boothroyd, Silvia N. Moreno PLoS ONE.2012; 7(2): e29998. CrossRef Toxoplasma gondii: Expression of GRA1 gene in endoplasmic reticulum promotes both growth and adherence and modulates intracellular calcium release in macrophages Jie Lin, Xu Lin, Guo-hua Yang, Yong Wang, Bi-wen Peng, Jian-yin Lin Experimental Parasitology.2010; 125(2): 165. CrossRef Schizophrenia Susceptibility Genes Directly Implicated in the Life Cycles of Pathogens: Cytomegalovirus, Influenza, Herpes simplex, Rubella, and Toxoplasma gondii C.J. Carter Schizophrenia Bulletin.2009; 35(6): 1163. CrossRef GRA Proteins of Toxoplasma gondii: Maintenance of Host-Parasite Interactions across the Parasitophorous Vacuolar Membrane Ho-Woo Nam The Korean Journal of Parasitology.2009; 47(Suppl): S29. CrossRef Interaction between Parasitophorous Vacuolar Membrane-associated GRA3 and Calcium Modulating Ligand of Host Cell Endoplasmic Reticulum in the Parasitism of Toxoplasma gondii Ji Yeon Kim, Hye-Jin Ahn, Kyung Ju Ryu, Ho-Woo Nam The Korean Journal of Parasitology.2008; 46(4): 209. CrossRef Nucleolar translocalization of GRA10 of Toxoplasma gondii transfectionally expressed in HeLa cells Hye-Jin Ahn, Sehra Kim, Ho-Woo Nam The Korean Journal of Parasitology.2007; 45(3): 165. CrossRef 12,174 View 108 Download Crossref Molecular cloning of a rhoptry protein (ROP6) secreted from Toxoplasma gondii Hye-Jin Ahn, Sehra Kim, Ho-Woo Nam Korean J Parasitol 2006;44(3):251-254. Published online September 20, 2006 DOI: https://doi.org/10.3347/kjp.2006.44.3.251 Abstract PDF Monoclonal antibody (mAb) Tg786 against Toxoplasma gondii has been found to detect a 42-kDa rhoptry protein (ROP6) which showed protease activity and host cell binding characteristics after secretion. Using the mAb, a colony containing a 3'-UTR was probed in a T. gondii cDNA expression library. A full length cDNA sequence of the rhoptry protein was completed after 5'-RACE, which consisted of 1,908 bp with a 1,443 bp ORF. The deduced amino acid sequence of ROP6 consisted of a polypeptide of 480 amino acids without significant homology to any other known proteins. This sequence contains an amino terminal stop transfer sequence downstream of a short neutral sequence, hydrophilic middle sequence, and hydrophobic carboxy terminus. It is suggested that the ROP6 is inserted into the rhoptry membrane with both N- and C-termini. Citations Citations to this article as recorded by A systematic review of Toxoplasma gondii antigens to find the best vaccine candidates for immunization Fatemeh Rezaei, Shahabeddin Sarvi, Mahdi Sharif, Seyed Hossein Hejazi, Abdol sattar Pagheh, Sargis A. Aghayan, Ahmad Daryani Microbial Pathogenesis.2019; 126: 172. CrossRef Toxoplasma gondii Secretory Proteins and their Role in Invasion and Pathogenesis Yang Zhang, Bo Shiun Lai, Mario Juhas, Yun Zhang Microbiological Research.2019;[Epub] CrossRef A systematic review on the role of GRA proteins of Toxoplasma gondii in host immunization Fatemeh Rezaei, Mahdi Sharif, Shahabeddin Sarvi, Seyed Hossein Hejazi, Sargis Aghayan, Abdol Sattar Pagheh, Samira Dodangeh, Ahmad Daryani Journal of Microbiological Methods.2019; 165: 105696. CrossRef Rhoptry protein 6 from Toxoplasma gondii is an intrinsically disordered protein Won-Kyu Lee, Hye-Jin Ahn, Yeon Gyu Yu, Ho-Woo Nam Protein Expression and Purification.2014; 101: 146. CrossRef Electrophoretic Patterns of Toxoplasma gondii Excreted/Secreted Antigens and Their Role in Induction of the Humoral Immune Response Ahmad Daryani, Mehdi Sharif, Hamed Kalani, Alireza Rafiei, Farzad Kalani, Ehsan Ahmadpour Jundishapur Journal of Microbiology.2014;[Epub] CrossRef Kiss and spit: the dual roles of Toxoplasma rhoptries John C. Boothroyd, Jean-Francois Dubremetz Nature Reviews Microbiology.2008; 6(1): 79. CrossRef Characterization of a New Gene <italic>wx2</italic> in <italic>Toxoplasma gondii</italic> Xiang Wu, Qiong Zhang, Kui Tan, Ronghua Xie, Jiubo Fan, Hengping Shu, Shiping Wang Acta Biochimica et Biophysica Sinica.2007; 39(7): 475. CrossRef 7,620 View 52 Download Crossref