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Sejoung Yoon	3 Articles
Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP Hyeng-Il Cheun, Kyungjin Kim, Sejoung Yoon, Won-Ja Lee, Woo-Yoon Park, Seobo Sim, Jae-Ran Yu Korean J Parasitol 2013;51(3):353-355. Published online June 30, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.3.353 Abstract PDF There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorphism (RFLP) method to distinguish between C. parvum and C. hominis. Using the CP2 gene as the target, we found that both Cryptosporidium species yielded 224 bp products. In the subsequent RFLP method using TaqI, 2 bands (99 and 125 bp) specific to C. hominis were detected. Using this method, we detected C. hominis infection in 1 of 21 patients with diarrhea, suggesting that this method could facilitate the detection of C. hominis infections. Citations Citations to this article as recorded by Causes of acute gastroenteritis in Korean children between 2004 and 2019 Eell Ryoo Clinical and Experimental Pediatrics.2021; 64(6): 260. CrossRef Cryptosporidium spp. Diagnosis and Research in the 21st Century Jennifer K. O'Leary, Roy D. Sleator, Brigid Lucey Food and Waterborne Parasitology.2021; 24: e00131. CrossRef Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis of Cryptosporidium spp. in Stool Samples Felix Weinreich, Andreas Hahn, Kirsten Alexandra Eberhardt, Torsten Feldt, Fred Stephen Sarfo, Veronica Di Cristanziano, Hagen Frickmann, Ulrike Loderstädt Pathogens.2021; 10(9): 1131. CrossRef Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers: Detection ofCryptosporidiumSpecies in Kenyan Children Presenting with Diarrhea Timothy S. Mamba, Cecilia K. Mbae, Johnson Kinyua, Erastus Mulinge, Gitonga Nkanata Mburugu, Zablon K. Njiru Journal of Tropical Medicine.2018; 2018: 1. CrossRef Expression and Purification of gp40/15 Antigen of Cryptosporidium parvum Parasite in Escherichia coli: an Innovative Approach in Vaccine Production Hossein Sobati, Habib Jasor-Gharebagh, Hossein Honari Iranian Red Crescent Medical Journal.2017;[Epub] CrossRef Detection of Cryptosporidium parvum in Environmental Soil and Vegetables Semie Hong, Kyungjin Kim, Sejoung Yoon, Woo-Yoon Park, Seobo Sim, Jae-Ran Yu Journal of Korean Medical Science.2014; 29(10): 1367. CrossRef 10,769 View 148 Download Crossref Comparison of Resistance to γ-Irradiation between Cryptosporidium parvum and Cryptosporidium muris Using In Vivo Infection Sejoung Yoon, Jae-Ran Yu Korean J Parasitol 2011;49(4):423-426. Published online December 16, 2011 DOI: https://doi.org/10.3347/kjp.2011.49.4.423 Abstract PDF In the genus Cryptosporidium, there are more than 14 species with different sizes and habitats, as well as different hosts. Among these, C. parvum and C. hominis are known to be human pathogens. As C. parvum can survive exposure to harsh environmental conditions, including various disinfectants or high doses of radiation, it is considered to be an important environmental pathogen that may be a threat to human health. However, the resistance of other Cryptosporidium species to various environmental conditions is unknown. In this study, resistance against γ-irradiation was compared between C. parvum and C. muris using in vivo infection in mice. The capability of C. muris to infect mice could be eliminated with 1,000 Gy of γ-irradiation, while C. parvum remained infective in mice after up to 1,000 Gy of γ-irradiation, although the peak number of oocysts per gram of feces decreased to 16% that of non-irradiated oocysts. The difference in radioresistance between these 2 Cryptosporidium species should be investigated by further studies. Citations Citations to this article as recorded by Apicomplexan parasites are attenuated by low-energy electron irradiation in an automated microfluidic system and protect against infection with Toxoplasma gondii Julia Finkensieper, Florian Mayerle, Zaida Rentería-Solís, Jasmin Fertey, Gustavo R. Makert, Franziska Lange, Joana Besecke, Simone Schopf, Andre Poremba, Ulla König, Bastian Standfest, Martin Thoma, Arwid Daugschies, Sebastian Ulbert Parasitology Research.2023; 122(8): 1819. CrossRef Recombinant thioredoxin peroxidase from Cryptosporidium parvum has more powerful antioxidant activity than that from Cryptosporidium muris Sejoung Yoon, Woo-Yoon Park, Jae-Ran Yu Experimental Parasitology.2012; 131(3): 333. CrossRef 8,588 View 64 Download Crossref Multiplex PCR Detection of Waterborne Intestinal Protozoa: Microsporidia, Cyclospora, and Cryptosporidium Seung-Hyun Lee, Migyo Joung, Sejoung Yoon, Kyoungjin Choi, Woo-Yoon Park, Jae-Ran Yu Korean J Parasitol 2010;48(4):297-301. Published online December 16, 2010 DOI: https://doi.org/10.3347/kjp.2010.48.4.297 Abstract PDF Recently, emerging waterborne protozoa, such as microsporidia, Cyclospora, and Cryptosporidium, have become a challenge to human health worldwide. Rapid, simple, and economical detection methods for these major waterborne protozoa in environmental and clinical samples are necessary to control infection and improve public health. In the present study, we developed a multiplex PCR test that is able to detect all these 3 major waterborne protozoa at the same time. Detection limits of the multiplex PCR method ranged from 10¹ to 10² oocysts or spores. The primers for microsporidia or Cryptosporidium used in this study can detect both Enterocytozoon bieneusi and Encephalitozoon intestinalis, or both Cryptosporidium hominis and Cryptosporidium parvum, respectively. Restriction enzyme digestion of PCR products with BsaBI or BsiEI makes it possible to distinguish the 2 species of microsporidia or Cryptosporidium, respectively. This simple, rapid, and cost-effective multiplex PCR method will be useful for detecting outbreaks or sporadic cases of waterborne protozoa infections. Citations Citations to this article as recorded by Development of a new multiplex PCR to detect fecal coccidian parasite Manish Katiyar, Reena Gulati, Nonika Rajkumari, Rakesh Singh Indian Journal of Gastroenterology.2023; 42(2): 241. CrossRef Use of DNA nanosensors based on upconverting nanoparticles for detection of Nosema bombycis by fluorescence resonance energy transfer Ping He, Hongli Chen, Jiancheng Sun, Qiang Wang, Xudong Tang, Yiling Zhang, Feng Zhu, Zhongyuan Shen Folia Microbiologica.2022; 67(3): 419. CrossRef Human Microsporidiosis: A Narrative Review of Serodiagnostic Tests Samaneh Abdolahi Khabisi International Journal of Infection.2022;[Epub] CrossRef First Identification and Genotyping of Enterocytozoon bieneusi and Prevalence of Encephalitozoon intestinalis in Patients with Acute Diarrhea in the Republic of Korea Ji-Young Kwon, Ji-Ye Seo, Tae-Yun Kim, Hee-Il Lee, Jung-Won Ju Pathogens.2021; 10(11): 1424. CrossRef Rapid diagnosis of parasitic diseases: current scenario and future needs S. Momčilović, C. Cantacessi, V. Arsić-Arsenijević, D. Otranto, S. Tasić-Otašević Clinical Microbiology and Infection.2019; 25(3): 290. CrossRef Assessment of the first commercial multiplex PCR kit (ParaGENIE Crypto-Micro Real-Time PCR) for the detection of Cryptosporidium spp., Enterocytozoon bieneusi, and Encephalitozoon intestinalis from fecal samples Florent Morio, Philippe Poirier, Yohann Le Govic, Adrien Laude, Stéphane Valot, Guillaume Desoubeaux, Nicolas Argy, Céline Nourrisson, Christelle Pomares, Marie Machouart, Frédéric Dalle, Françoise Botterel, Nathalie Bourgeois, Estelle Cateau, Marion Lete Diagnostic Microbiology and Infectious Disease.2019; 95(1): 34. CrossRef Microfluidic multi-angle laser scattering system for rapid and label-free detection of waterborne parasites Wei Huang, Limei Yang, Gang Yang, Feng Li Biomedical Optics Express.2018; 9(4): 1520. CrossRef Opportunistic and non-opportunistic intestinal parasites in HIV/ AIDS patients in relation to their clinical and epidemiological status in a specialized medical service in Goiás, Brazil Natane Barbosa Barcelos, Lorena de Freitas e Silva, Regyane Ferreira Guimarães Dias, Hélio Ranes de Menezes Filho, Rosângela Maria Rodrigues Revista do Instituto de Medicina Tropical de São Paulo.2018;[Epub] CrossRef Detection Methodologies for Pathogen and Toxins: A Review Md Alahi, Subhas Mukhopadhyay Sensors.2017; 17(8): 1885. CrossRef Detection and Molecular Characterization of <i>Cryptosporidium</i> spp. from Wild Rodents and Insectivores in South Korea Juha Song, C-Yoon Kim, Seo-Na Chang, Tamer Said Abdelkader, Juhee Han, Tae-Hyun Kim, Hanseul Oh, Ji Min Lee, Dong-Su Kim, Jong-Taek Kim, Hong-Shik Oh, Moonsuk Hur, Jae-Hwa Suh, Jae-Hak Park The Korean Journal of Parasitology.2015; 53(6): 737. CrossRef Detection of Nosema bombycis by FTA Cards and Loop-Mediated Isothermal Amplification (LAMP) Wei Yan, Zhongyuan Shen, Xudong Tang, Li Xu, Qianlong Li, Yajie Yue, Shengyan Xiao, Xuliang Fu Current Microbiology.2014; 69(4): 532. CrossRef Molecular Analysis of the Alpha-Tubulin Gene from the Microsporidium, Endoreticulatus sp. Zhenjiang, Isolated from Bombyx mori Shengyan Xiao, Zhongyuan Shen, Xudong Tang, Li Xu, Xuliang Fu, Yajie Yue, Nan Li, Wei Wang Agricultural Sciences.2014; 05(10): 882. CrossRef Molecular Testing for Clinical Diagnosis and Epidemiological Investigations of Intestinal Parasitic Infections Jaco J. Verweij, C. Rune Stensvold Clinical Microbiology Reviews.2014; 27(2): 371. CrossRef Intestinal protozoan infections in the immunocompromised host Luis A. Marcos, Eduardo Gotuzzo Current Opinion in Infectious Diseases.2013; 26(4): 295. CrossRef Evaluation of formalin-ether sedimentation and trichrome staining techniques: Its effectiveness in detecting Entamoeba histolytica/dispar/moshkovskii in stool samples Tengku Shahrul Anuar, Hesham M. Al-Mekhlafi, Mohamed Kamel Abdul Ghani, Edariah Abu Bakar, Siti Nor Azreen, Fatmah Md Salleh, Norhayati Moktar Journal of Microbiological Methods.2013; 92(3): 344. CrossRef Epidemiological Characteristics of the First Water-Borne Outbreak of Cryptosporidiosis in Seoul, Korea Shinje Moon, Wooseok Kwak, Sangwon Lee, Won Kim, Jaeyeon Oh, Seung-Ki Youn Journal of Korean Medical Science.2013; 28(7): 983. CrossRef Zinc PVA versus potassium dichromate for preservation of microsporidian spores of human origin Hend Aly El-Taweel, Mona Mohammed Tolba, Hayam Abdelmonem Sadaka, Lobna Abdelaziz El-Zawawy, Mervat Mostafa Osman Parasitology Research.2012; 111(2): 689. CrossRef Microsporidiosis Elizabeth S. Didier, Louis M. Weiss Current Opinion in Infectious Diseases.2011; 24(5): 490. 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