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Selma Giorgio	2 Articles
Use of In Vivo and In Vitro Systems to Select Leishmania amazonensis Expressing Green Fluorescent Protein Solange dos Santos Costa, Marjorie de Assis Golim, Bartira Rossi-Bergmann, Fabio Trindade Maranh?o Costa, Selma Giorgio Korean J Parasitol 2011;49(4):357-364. Published online December 16, 2011 DOI: https://doi.org/10.3347/kjp.2011.49.4.357 Abstract PDF Various Leishmania species were engineered with green fluorescent protein (GFP) using episomal vectors that encoded an antibiotic resistance gene, such as aminoglycoside geneticin sulphate (G418). Most reports of GFP-Leishmania have used the flagellated extracellular promastigote, the stage of parasite detected in the midgut of the sandfly vector; fewer studies have been performed with amastigotes, the stage of parasite detected in mammals. In this study, comparisons were made regarding the efficiency for in vitro G418 selection of GFP-Leishmania amazonensis promastigotes and amastigotes and the use of in vivo G418 selection. The GFP-promastigotes retained episomal plasmid for a prolonged period and G418 treatment was necessary and efficient for in vitro selection. In contrast, GFP-amastigotes showed low retention of the episomal plasmid in the absence of G418 selection and low sensitivity to antibiotics in vitro. The use of protocols for G418 selection using infected BALB/c mice also indicated low sensitivity to antibiotics against amastigotes in cutaneous lesions. Citations Citations to this article as recorded by Nanocrystallization Effectively Improves the Oral Efficacy of an Antileishmanial Chalcone Maria Paula Gonçalves Borsodi, Wallace Pacienza-Lima, Jaqueline Correia Villaça Menezes, Douglas Escrivani-Oliveira, Natalia Arruda-Costa, Alcides José Monteiro da Silva, Lucio Mendes Cabral, Patrick G. Steel, Ariane de Jesus Sousa-Batista, Bartira Rossi- Pharmaceutics.2025; 17(4): 399. CrossRef Reporter gene systems: A powerful tool for Leishmania studies Romário Lopes Boy, Ahyun Hong, Juliana Ide Aoki, Lucile Maria Floeter-Winter, Maria Fernanda Laranjeira-Silva Current Research in Microbial Sciences.2022; 3: 100165. CrossRef In Vivo Safety and Efficacy of Chalcone-Loaded Microparticles with Modified Polymeric Matrix against Cutaneous Leishmaniasis Ariane de J. Sousa-Batista, Natalia Arruda-Costa, Wallace Pacienza-Lima, Felipe Carvalho-Gondim, Rosiane F. Santos, Silvia A. G. Da-Silva, Maria Inês Ré, Bartira Rossi-Bergmann Pharmaceutics.2022; 15(1): 51. CrossRef Antileishmanial Chemotherapy through Clemastine Fumarate Mediated Inhibition of the Leishmania Inositol Phosphorylceramide Synthase John G. M. Mina, Rebecca L. Charlton, Edubiel Alpizar-Sosa, Douglas O. Escrivani, Christopher Brown, Amjed Alqaisi, Maria Paula G. Borsodi, Claudia P. Figueiredo, Emanuelle V. de Lima, Emily A. Dickie, Wenbin Wei, Robson Coutinho-Silva, Andy Merritt, Terr ACS Infectious Diseases.2021; 7(1): 47. CrossRef Ex Vivo Phenotypic Screening of Two Small Repurposing Drug Collections Identifies Nifuratel as a Potential New Treatment against Visceral and Cutaneous Leishmaniasis Bárbara Domínguez-Asenjo, Camino Gutiérrez-Corbo, María Álvarez-Bardón, Yolanda Pérez-Pertejo, Rafael Balaña-Fouce, Rosa M. Reguera ACS Infectious Diseases.2021; 7(8): 2390. CrossRef Encapsulation in lipid-core nanocapsules improves topical treatment with the potent antileishmanial compound CH8 Douglas O Escrivani, Milene Valéria Lopes, Fernanda Poletto, Stela Regina Ferrarini, Ariane J. Sousa-Batista, Patrick G. Steel, Sílvia Stanisçuaski Guterres, Adriana Raffin Pohlmann, Bartira Rossi-Bergmann Nanomedicine: Nanotechnology, Biology and Medicine.2020; 24: 102121. CrossRef Single-dose treatment for cutaneous leishmaniasis with an easily synthesized chalcone entrapped in polymeric microparticles Ariane J. Sousa-Batista, Natalia Arruda-Costa, Douglas O. Escrivani, Franceline Reynaud, Patrick G. Steel, Bartira Rossi-Bergmann Parasitology.2020; 147(9): 1032. CrossRef Broad Spectrum and Safety of Oral Treatment with a Promising Nitrosylated Chalcone in Murine Leishmaniasis Ariane J. Sousa-Batista, Douglas Escrivani-Oliveira, Camila Alves Bandeira Falcão, Cintia Iana Monteiro da Silva Philipon, Bartira Rossi-Bergmann Antimicrobial Agents and Chemotherapy.2018;[Epub] CrossRef EGFP reporter protein: its immunogenicity in Leishmania-infected BALB/c mice Samira Seif, Fereshteh Kazemi, Elham Gholami, Negar Seyed, Yasaman Taslimi, Sima Habibzadeh, Bahareh Azarian, Shahram Jamshidi, Mehrdad Hashemi, Sima Rafati, Tahereh Taheri Applied Microbiology and Biotechnology.2016; 100(9): 3923. CrossRef Development of Leishmania donovani stably expressing DsRed for flow cytometry-based drug screening using chalcone thiazolyl-hydrazone as a new antileishmanial target Anil Kumar Jaiswal, K. Bhaskara Rao, Pragati Kushwaha, Keerti Rawat, Ram K. Modukuri, Prashant Khare, Sumit Joshi, Shikha Mishra, Ambak Rai, Koneni V. Sashidhara, Anuradha Dube International Journal of Antimicrobial Agents.2016; 48(6): 695. CrossRef Evaluation of the leishmanicidal and cytotoxic effects of inhibitors for microorganism metabolic pathway enzymes Ademar de Mesquita Barbosa, Solange dos Santos Costa, Josmar Rodrigues da Rocha, Carlos Alberto Montanari, Selma Giorgio Biomedicine & Pharmacotherapy.2015; 74: 95. CrossRef An alternative in vitro drug screening test using Leishmania amazonensis transfected with red fluorescent protein Marcele N. Rocha, Célia M. Corrêa, Maria N. Melo, Stephen M. Beverley, Olindo Assis Martins-Filho, Ana Paula Madureira, Rodrigo P. Soares Diagnostic Microbiology and Infectious Disease.2013; 75(3): 282. CrossRef 9,283 View 84 Download Crossref A Novel Organotellurium Compound (RT-01) as a New Antileishmanial Agent Camila B?rbara Cantalupo Lima, Wagner Welber Arrais-Silva, Rodrigo Luiz Oliveira Rodrigues Cunha, Selma Giorgio Korean J Parasitol 2009;47(3):213-218. Published online August 28, 2009 DOI: https://doi.org/10.3347/kjp.2009.47.3.213 Abstract PDF Leishmaniasis is a neglected disease and endemic in developing countries. A lack of adequate and definitive chemotherapeutic agents to fight against this infection has led to the investigation of numerous compounds. The aim of this study was to investigate the effect of RT-01, an organotellurane compound presenting biological activities, in 2 experimental systems against Leishmania amazonensis. The in vitro system consisted of promastigotes and amastigotes forms of the parasite, and the in vivo system consisted of L. amazonensis infected BALB/c mice, an extremely susceptible mouse strain. The compound proved to be toxic against promastigotes and amastigotes. The study also showed that treatment with RT-01 produces an effect similar to that treatment with the reference antimonial drug, Glucantime, in L. amazonensis infected mice. The best results were obtained following RT-01 intralesional administration (720 ?g/kg/day); mice showed significant delay in the development of cutaneous lesions and decreased numbers of parasites obtained from the lesions. Significant differences in tissue pathology consisted mainly of no expressive accumulation of inflammatory cells and well-preserved structures in the skin tissue of RT-01-treated mice compared with expressive infiltration of infected cells replacing the skin tissue in lesions of untreated mice. These findings highlight the fact that the apparent potency of organotellurane compounds, together with their relatively simple structure, may represent a new avenue for the development of novel drugs to combat parasitic diseases. Citations Citations to this article as recorded by A blending-based therapeutic strategy using diaryl dichalcogenides and ivermectin against gastrointestinal nematodes of small ruminants Irineu Romero-Neto, Júlia Dall’Anese, Tay Zugman, Eduarda Proença, Alda Lúcia Gomes Monteiro, Leandro Piovan, Marcelo Beltrão Molento Veterinary Parasitology.2025; 338: 110529. CrossRef Indazole Derivatives Against Murine Cutaneous Leishmaniasis Niurka Mollineda-Diogo, Yunierkis Pérez-Castillo, Sergio Sifontes-Rodríguez, Osmani Marrero-Chang, Alfredo Meneses-Marcel, Alma Reyna Escalona-Montaño, María Magdalena Aguirre-García, Teresa Espinosa-Buitrago, Yeny Morales-Moreno, Vicente Arán-Redó Pharmaceuticals.2025; 18(8): 1107. CrossRef Analysis of thermomechanical coupled accelerated aging of HTPB propellants Yi Zeng, Wei Huang, Jia‐Xing Chen, Jin‐Sheng Xu, Xiong Chen, Rui Wu, Qi‐Xuan Song Propellants, Explosives, Pyrotechnics.2024;[Epub] CrossRef Synthesis and application of organotellurium compounds Biswa Mohan Sahoo, Bimal Krishna Banik, Abhishek Tiwari, Varsha Tiwari, Adya Jain, Preetismita Borah Physical Sciences Reviews.2023; 8(12): 4435. CrossRef Synthesis of Tellurium Oxide (TeO2) Nanorods and Nanoflakes and Evaluation of Its Efficacy Against Leishmania major In Vitro and In Vivo Pooya Tavakoli, Fatemeh Ghaffarifar, Hamid Delavari, Amir KarimiPourSaryazdi, Mohammad Saaid Dayer, Vahid Nasiri, Salimeh Ahmadi Acta Parasitologica.2022; 67(1): 143. CrossRef Equilibrium between tri‐ and tetra‐coordinate chalcogenuranes is critical for cysteine protease inhibition Gabriela Dias‐da‐Silva, Rodrigo L. O. R. Cunha, Mauricio D. Coutinho‐Neto Journal of Computational Chemistry.2021; 42(17): 1225. CrossRef Synthesis, Mechanism Elucidation and Biological Insights of Tellurium(IV)‐Containing Heterocycles João Pedro A. Souza, Leociley R. A. Menezes, Francielle P. Garcia, Débora B. Scariot, Pamela T. Bandeira, Mateus B. Bespalhok, Siddhartha O. K. Giese, David L. Hughes, Celso V. Nakamura, Andersson Barison, Alfredo R. M. Oliveira, Renan B. Campos, Leandro Chemistry – A European Journal.2021; 27(58): 14427. CrossRef Stereoselective Bioreduction of Telluro‐Acetophenones to Optically Active Hydroxy Tellurides Pamela Taisline Bandeira, Vicente Gotor‐Fernández, Leandro Piovan European Journal of Organic Chemistry.2020; 2020(9): 1129. CrossRef Tellurides Bearing Sulfonamides as Novel Inhibitors of Leishmanial Carbonic Anhydrase with Potent Antileishmanial Activity Andrea Angeli, Mikel Etxebeste-Mitxeltorena, Carmen Sanmartín, Socorro Espuelas, Esther Moreno, Amaya Azqueta, Seppo Parkkila, Fabrizio Carta, Claudiu T. Supuran Journal of Medicinal Chemistry.2020; 63(8): 4306. CrossRef preADMET analysis and clinical aspects of dogs treated with the Organotellurium compound RF07: A possible control for canine visceral leishmaniasis? Adriana Maria Viana Nunes, Francisco das Chagas Pereira de Andrade, Lívia Alves Filgueiras, Octávio Augusto de Carvalho Maia, Rodrigo L.O.R. Cunha, Sindy V.A. Rodezno, Antônio Luiz Martins Maia Filho, Fernando Aécio de Amorim Carvalho, Debora Cavalcante B Environmental Toxicology and Pharmacology.2020; 80: 103470. CrossRef Metal Compounds against Neglected Tropical Diseases Yih Ching Ong, Saonli Roy, Philip C. Andrews, Gilles Gasser Chemical Reviews.2019; 119(2): 730. CrossRef Antitumor effect of chiral organotelluranes elicited in a murine melanoma model Thaysa Paschoalin, Adam A. Martens, Álvaro T. Omori, Felipe V. Pereira, Luiz Juliano, Luiz R. Travassos, Glaucia M. Machado-Santelli, Rodrigo L.O.R. Cunha Bioorganic & Medicinal Chemistry.2019; 27(12): 2537. CrossRef Diacetal Ditellurides as Highly Active and Selective Antiparasitic Agents toward Leishmania amazonensis Pamela T. Bandeira, João Pedro A. Souza, Débora B. Scariot, Francielle P. Garcia, Celso V. Nakamura, Alfredo R. M. de Oliveira, Leandro Piovan ACS Medicinal Chemistry Letters.2019; 10(5): 806. CrossRef Ammonium trichloro [1,2-ethanediolato-O,O′]-tellurate cures experimental visceral leishmaniasis by redox modulation of Leishmania donovani trypanothione reductase and inhibiting host integrin linked PI3K/Akt pathway Preeti Vishwakarma, Naveen Parmar, Pragya Chandrakar, Tanuj Sharma, Manoj Kathuria, Pramod K. Agnihotri, Mohammad Imran Siddiqi, Kalyan Mitra, Susanta Kar Cellular and Molecular Life Sciences.2018; 75(3): 563. CrossRef Resuspendable Powders of Lyophilized Chalcogen Particles with Activity against Microorganisms Sharoon Griffin, Muhammad Sarfraz, Steffen Hartmann, Shashank Pinnapireddy, Muhammad Nasim, Udo Bakowsky, Cornelia Keck, Claus Jacob Antioxidants.2018; 7(2): 23. CrossRef Synthesis, spectral characterization, DFT studies and biological activity of novel Ligand 1‐(2‐cyclohexyl thioethyl) piperidine and its complexes with group 12 metal chlorides Muzzaffar A. Bhat, Shabir H. Lone, Muzzaffar A. Mir, Sheikh A. Majid, Sanjay K. Srivastava Applied Organometallic Chemistry.2018;[Epub] CrossRef Cysteine proteases in protozoan parasites Jair L. Siqueira-Neto, Anjan Debnath, Laura-Isobel McCall, Jean A. Bernatchez, Momar Ndao, Sharon L. Reed, Philip J. Rosenthal, Photini Sinnis PLOS Neglected Tropical Diseases.2018; 12(8): e0006512. CrossRef Glucose-6-phosphate dehydrogenase and Trypanothione reductase interaction protects Leishmania donovani from metalloid mediated oxidative stress Ayan Kumar Ghosh, Savita Saini, Sushmita Das, Abhishek Mandal, Abul Hasan Sardar, Md. Yousuf Ansari, Kumar Abhishek, Ajay Kumar, Ruby Singh, Sudha Verma, Asif Equbal, Vahab Ali, Pradeep Das Free Radical Biology and Medicine.2017; 106: 10. CrossRef Stability Study of Hypervalent Tellurium Compounds in Aqueous Solutions Cleverson R. Princival, Marcos V. L. R. Archilha, Alcindo A. Dos Santos, Maurício P. Franco, Ataualpa A. C. Braga, André F. Rodrigues-Oliveira, Thiago C. Correra, Rodrigo L. O. R. Cunha, João V. Comasseto ACS Omega.2017; 2(8): 4431. CrossRef Thioridazine inhibits gene expression control of the cell wall signaling pathway (CWI) in the human pathogenic fungus Paracoccidioides brasiliensis Daniela Leite Jabes, Ana Claudia de Freitas Oliveira, Valquíria Campos Alencar, Fabiano Bezerra Menegidio, Débora Liliane Souza Reno, Daiene Souza Santos, David Aciole Barbosa, Renata Ozelami Vilas Boas, Rodrigo Luiz de Oliveira Rodrigues Cunha, Tiago Rod Molecular Genetics and Genomics.2016; 291(3): 1347. CrossRef The Anticancer Activity of Organotelluranes: Potential Role in Integrin Inactivation Alon Silberman, Yona Kalechman, Shira Hirsch, Ziv Erlich, Benjamin Sredni, Amnon Albeck ChemBioChem.2016; 17(10): 918. CrossRef Synthesis and Characterization of 2-Fluoro-3-Pyridyl Tellurium Compounds: X-Ray Crystal Structure of Bis(2-Fluoro-3-Pyridyltelluro)Methane Shivani Gulati (nee Doomra), Shikha Pundir, Sanjeev Kumar, Kuldip K. Bhasin Phosphorus, Sulfur, and Silicon and the Related Elements.2015; 190(9): 1509. CrossRef Evaluation of the leishmanicidal and cytotoxic effects of inhibitors for microorganism metabolic pathway enzymes Ademar de Mesquita Barbosa, Solange dos Santos Costa, Josmar Rodrigues da Rocha, Carlos Alberto Montanari, Selma Giorgio Biomedicine & Pharmacotherapy.2015; 74: 95. CrossRef Synthesis, antimicrobial activity, computational and modeling studies of some new organotellurium compounds containing azo moities Wasfi Aboud Al-Masoudi, Rafid Hmedan Al-Asadi, Rasha Munther Othman, Najim Aboud Al‐Masoudi European Journal of Chemistry.2015; 6(4): 374. CrossRef Antileishmanial activity of essential oil from Chenopodium ambrosioides and its main components against experimental cutaneous leishmaniasis in BALB/c mice L. Monzote, J. Pastor, R. Scull, L. Gille Phytomedicine.2014; 21(8-9): 1048. CrossRef Triphenyltellurium chloride Ambika Chopra, Shalini Jain, Sanjay K. Srivastava, Sushil K. Gupta, Ray J. 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