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Soon Cheol Ahn	4 Articles
Trichinella spiralis Infection Suppressed Gut Inflammation with CD4⁺CD25⁺Foxp3⁺ T Cell Recruitment Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Seon Hee Choi, Soon Cheol Ahn, Hak Sun Yu Korean J Parasitol 2012;50(4):385-390. Published online November 26, 2012 DOI: https://doi.org/10.3347/kjp.2012.50.4.385 Abstract PDF In order to know the effect of pre-existing Trichinella spiralis infection on experimentally induced intestinal inflammation and immune responses, we induced colitis in T. spiralis-infected mice and observed the severity of colitis and the levels of Th1, Th2, and regulatory cytokines and recruitment of CD4⁺CD25⁺Foxp3⁺ T (regulatory T; T_reg) cells. Female C57BL/6 mice were infected with 250 muscle larvae; after 4 weeks, induction of experimental colitis was performed using 3% dextran sulfate sodium (DSS). During the induction period, we observed severity of colitis, including weight loss and status of stool, and evaluated the disease activity index (DAI). A significantly low DAI and degree of weight loss were observed in infected mice, compared with uninfected mice. In addition, colon length in infected mice was not contracted, compared with uninfected mice. We also observed a significant increase in production of pro-inflammatory cytokines, IL-6 and IFN-γ, in spleen lymphocytes treated with DSS; however, such an increase was not observed in infected mice treated with DSS. Of particular interest, production of regulatory cytokines, IL-10 and transforming growth factor (TGF)-β, in spleen lymphocytes showed a significant increase in mice infected with T. spiralis. A similar result was observed in mesenteric lymph nodes (MLN). Subsets of the population of T_reg cells in MLN and spleen showed significant increases in mice infected with T. spiralis. In conclusion, T. spiralis infection can inhibit the DSS-induced colitis in mice by enhancing the regulatory cytokine and T_reg cells recruitment. Citations Citations to this article as recorded by Mechanism of Trichinella spiralis-mediated CD4+ T cell functional imbalance via IDO-dependent tryptophan metabolism Wenhao Yu, Xuhong Yuan, Wenqi Li, Caixia Han International Immunopharmacology.2026; 177: 116517. CrossRef Helminths in alternative therapeutics of inflammatory bowel disease Himani Pandey, Daryl W. T. Tang, Sunny H. Wong, Devi Lal Intestinal Research.2025; 23(1): 8. CrossRef Cathepsin L of Fasciola hepatica meliorates colitis by altering the gut microbiome and inflammatory macrophages Jing-Zhi Gong, Jun-Jie Huang, Ming Pan, Qi-Wang Jin, Yi-Min Fan, Wen-Qian Shi, Si-Yang Huang International Journal of Biological Macromolecules.2025; 286: 138270. CrossRef Improved antiparasitic effects of mebendazole using chitosan and zinc oxide nanocomposites for drug delivery in Trichinella spiralis infected mice during the muscular phase Nabila M. Mira, Aya M. Henaish, Eman A. Moussa, Ibrahim B. Helal, Shaimaa M. Kasem Acta Tropica.2025; 263: 107565. CrossRef Therapeutic potentials of Trichinella spiralis in immune disorders: From allergy to autoimmunity Minkyoung Cho, Hak Sun Yu Parasites, Hosts and Diseases.2025; 63(2): 123. CrossRef Trichinella-derived protein ameliorates colitis by altering the gut microbiome and improving intestinal barrier function Shao Rong Long, Wen Xuan Shang, Hui Ran Zhang, Miao Jiang, Jing Jing Wang, Ruo Dan Liu, Zhong Quan Wang, Jing Cui, Hualei Sun International Immunopharmacology.2024; 127: 111320. CrossRef The gut microbiota is essential for Trichinella spiralis—evoked suppression of colitis Hualei Sun, Shao Rong Long, Miao Jiang, Hui Ran Zhang, Jing Jing Wang, Zi Xuan Liao, Jing Cui, Zhong Quan Wang, Subbaraman Babu PLOS Neglected Tropical Diseases.2024; 18(11): e0012645. CrossRef The Underrated Gut Microbiota Helminths, Bacteriophages, Fungi, and Archaea Maria Jose Garcia-Bonete, Anandi Rajan, Francesco Suriano, Elena Layunta Life.2023; 13(8): 1765. CrossRef Helminth-derived biomacromolecules as therapeutic agents for treating inflammatory and infectious diseases: What lessons do we get from recent findings? Pritha Chakraborty, Vivekanandhan Aravindhan, Suprabhat Mukherjee International Journal of Biological Macromolecules.2023; 241: 124649. CrossRef Potential of human helminth therapy for resolution of inflammatory bowel disease: The future ahead Toshio Arai, Fernando Lopes Experimental Parasitology.2022; 232: 108189. CrossRef Trichinella-induced immunomodulation: Another tale of helminth success F. Bruschi, D.S. Ashour, A.A. Othman Food and Waterborne Parasitology.2022; 27: e00164. CrossRef Trichinella Infection Ameliorated Vincristine-Induced Neuroinflammation in Mice Young Rae Jo, Hwan Tae Park, Hak Sun Yu, Hyun-Hee Kong The Korean Journal of Parasitology.2022; 60(4): 247. CrossRef Immune Protection of a Helminth Protein in the DSS-Induced Colitis Model in Mice Shao Rong Long, Ruo Dan Liu, Deepak Vijaya Kumar, Zhong Quan Wang, Chien-Wen Su Frontiers in Immunology.2021;[Epub] CrossRef Trichinella spiralis:inflammation modulator Jing Ding, Xiaolei Liu, Xue Bai, Yang Wang, Jian Li, Chun Wang, Shicun Li, Mingyuan Liu, Xuelin Wang Journal of Helminthology.2020;[Epub] CrossRef Infection with Toxocara canis Inhibits the Production of IgE Antibodies to α-Gal in Humans: Towards a Conceptual Framework of the Hygiene Hypothesis? Adnan Hodžić, Lourdes Mateos-Hernández, Emilie Fréalle, Patricia Román-Carrasco, Pilar Alberdi, Muriel Pichavant, Veronica Risco-Castillo, Delphine Le Roux, Jérôme Vicogne, Wolfgang Hemmer, Herbert Auer, Ines Swoboda, Georg Duscher, José de la Fuente, Ale Vaccines.2020; 8(2): 167. CrossRef Exposure time determines the protective effect of Trichinella spiralis on experimental colitis Wenxiao Zheng, Zhenrong Ma, Xi Sun, Yehong Huang, Bin Lu, Xiaogang Chen, Xiang Xue, Xuexian Yang, Xiang Wu Microbial Pathogenesis.2020; 147: 104263. CrossRef Effect of Trichinella spp. or derived antigens on chemically induced inflammatory bowel disease (IBD) in mouse models: A systematic review and meta-analysis Jian Li, Xiaolei Liu, Jing Ding, Bin Tang, Xue Bai, Yang Wang, Shicun Li, Mingyuan Liu, Xuelin Wang International Immunopharmacology.2020; 85: 106646. CrossRef Adoptive transfer of Trichinella spiralis-activated macrophages can ameliorate both Th1- and Th2-activated inflammation in murine models Shin Ae Kang, Mi-Kyung Park, Sang Kyun Park, Jun Ho Choi, Da In Lee, So Myong Song, Hak Sun Yu Scientific Reports.2019;[Epub] CrossRef Acute infection with Strongyloides venezuelensis increases intestine production IL-10, reduces Th1/Th2/Th17 induction in colon and attenuates Dextran Sulfate Sodium-induced colitis in BALB/c mice Vanessa Fernandes Rodrigues, Márcia Paulliny Soares Bahia, Núbia Rangel Cândido, João Marcelo Peixoto Moreira, Vinicius Gustavo Oliveira, Emília Souza Araújo, Jailza Lima Rodrigues Oliveira, Michelle de Carvalho Rezende, Ary Correa, Deborah Negrão-Corrêa Cytokine.2018; 111: 72. CrossRef Regulatory T Cell and Forkhead Box Protein 3 as Modulators of Immune Homeostasis Leonn Mendes Soares Pereira, Samara Tatielle Monteiro Gomes, Ricardo Ishak, Antonio Carlos Rosário Vallinoto Frontiers in Immunology.2017;[Epub] CrossRef Ascaris suum infection modulates inflammation: Implication of CD4+ CD25high Foxp3+ T cells and IL‐10 T. de O. Titz, C. A. A. de Araújo, C. S. Enobe, P. O. Rigato, T. M. Oshiro, M. F. de Macedo‐Soares Parasite Immunology.2017;[Epub] CrossRef Biodegradable Chitosan Decreases the Immune Response to Trichinella spiralis in Mice Klaudia Brodaczewska, Natalia Wolaniuk, Katarzyna Lewandowska, Katarzyna Donskow-Łysoniewska, Maria Doligalska Molecules.2017; 22(11): 2008. CrossRef *Activation and Recruitment of Regulatory T Cells via Chemokine Receptor Activation in Trichinella spiralis-Infected Mice* Jeong-Bin Ahn, Shin Ae Kang, Dong-Hee Kim, Hak Sun Yu The Korean Journal of Parasitology.2016; 54(2): 163. CrossRef Regulatory parameters of the lung immune response during the early phase of experimental trichinellosis Guido H. Falduto, Cecilia C. Vila, María P. Saracino, María V. Gentilini, Stella M. Venturiello Veterinary Parasitology.2016; 231: 47. CrossRef Helminth Regulation of Immunity Fernando Lopes, Chelsea Matisz, José L. Reyes, Humberto Jijon, Ahmed Al-Darmaki, Gilaad G. Kaplan, Derek M. McKay Inflammatory Bowel Diseases.2016; 22(10): 2499. CrossRef Immunomodulatory potential of particular Trichinella spiralis muscle larvae excretory–secretory components J. Cvetkovic, Lj. Sofronic-Milosavljevic, N. Ilic, M. Gnjatovic, I. Nagano, A. Gruden-Movsesijan International Journal for Parasitology.2016; 46(13-14): 833. CrossRef Allergenicity of two Anisakis simplex allergens evaluated in vivo using an experimental mouse model Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Maria Luisa Caballero, Teresa Perez-Pinar, Rosa Rodriguez-Perez, Mee Sun Ock, Hee Jae Cha, Yeon Chul Hong, Hak Sun Yu Experimental Parasitology.2014; 146: 71. CrossRef Excretory/Secretory Products from Trichinella spiralis Adult Worms Ameliorate DSS-Induced Colitis in Mice Xiaodi Yang, Yaping Yang, Yunyun Wang, Bin Zhan, Yuan Gu, Yuli Cheng, Xinping Zhu, Udai P. Singh PLoS ONE.2014; 9(5): e96454. CrossRef Trichinella spiralisimmunomodulation: an interactive multifactorial process Dalia S Ashour Expert Review of Clinical Immunology.2013; 9(7): 669. CrossRef 10,501 View 94 Download Crossref Protease-Activated Receptor 2 Is Involved in Th2 Responses against Trichinella spiralis Infection Mi Kyung Park, Min Kyoung Cho, Shin Ae Kang, Hye-Kyung Park, Yun Seong Kim, Ki Uk Kim, Soon Cheol Ahn, Dong-Hee Kim, Hak Sun Yu Korean J Parasitol 2011;49(3):235-243. Published online September 30, 2011 DOI: https://doi.org/10.3347/kjp.2011.49.3.235 Abstract PDF In order to get a better understanding of the role of protease-activated receptor 2 (PAR2) in type 2 helper T (Th2) cell responses against Trichinella spiralis infection, we analyzed Th2 responses in T. spiralis-infected PAR2 knockout (KO) mice. The levels of the Th2 cell-secreted cytokines, IL-4, IL-5, and IL-13 were markedly reduced in the PAR2 KO mice as compared to the wild type mice following infection with T. spiralis. The serum levels of parasite-specific IgE increased significantly in the wild type mice as the result of T. spiralis infection, but this level was not significantly increased in PAR2 KO mice. The expression level of thymic stromal lymphopoietin, IL-25, and eotaxin gene (the genes were recently known as Th2 response initiators) of mouse intestinal epithelial cells were increased as the result of treatment with T. spiralis excretory-secretory proteins. However, the expression of these chemokine genes was inhibited by protease inhibitor treatments. In conclusion, PAR2 might involve in Th2 responses against T. spiralis infection. Citations Citations to this article as recorded by Innate Immune Recognition of Helminths: TLRs and Beyond Camila de Almeida Lopes, Linda Djune Yemeli, Pedro Gazzinelli‐Guimaraes Parasite Immunology.2025;[Epub] CrossRef A novel trypsin of Trichinella spiralis mediates larval invasion of gut epithelium via binding to PAR2 and activating ERK1/2 pathway Lu Lu Han, Qi Qi Lu, Wen Wen Zheng, Yang Li Li, Yan Yan Song, Xin Zhuo Zhang, Shao Rong Long, Ruo Dan Liu, Zhong Quan Wang, Jing Cui, Krystyna Cwiklinski PLOS Neglected Tropical Diseases.2024; 18(1): e0011874. CrossRef Pathogenesis ofBrucellaepididymoorchitis-game ofBrucelladeath Jiuwang Yu, Sha Li, Lu Wang, Zhiheng Dong, Lengge Si, Lidao Bao, Lan Wu Critical Reviews in Microbiology.2022; 48(1): 96. CrossRef Cytokines and beyond: Regulation of innate immune responses during helminth infection Oyebola O. Oyesola, Simon P. Früh, Lauren M. Webb, Elia D. Tait Wojno Cytokine.2020; 133: 154527. CrossRef Animal Models for Functional Gastrointestinal Disorders Alison Accarie, Tim Vanuytsel Frontiers in Psychiatry.2020;[Epub] CrossRef Adoptive transfer of Trichinella spiralis-activated macrophages can ameliorate both Th1- and Th2-activated inflammation in murine models Shin Ae Kang, Mi-Kyung Park, Sang Kyun Park, Jun Ho Choi, Da In Lee, So Myong Song, Hak Sun Yu Scientific Reports.2019;[Epub] CrossRef The role of rare innate immune cells in Type 2 immune activation against parasitic helminths LAUREN M. WEBB, ELIA D. TAIT WOJNO Parasitology.2017; 144(10): 1288. CrossRef The induction of the collagen capsule synthesis by Trichinella spiralis is closely related to protease-activated receptor 2 Mi Kyung Park, Min Kyoung Cho, Shin Ae Kang, Bo Young Kim, Hak Sun Yu Veterinary Parasitology.2016; 230: 56. CrossRef Human Helminths and Allergic Disease: The Hygiene Hypothesis and Beyond Thomas B. Nutman, Helton C. Santiago The American Journal of Tropical Medicine and Hygiene.2016; 95(4): 746. CrossRef The role of infection in the pathogenesis of allergodermatoses E. V. Svirshchevskaya, E. V. Matushevskaya, D. B. Chudakov, Yu. I. Matushevskaya Klinicheskaya dermatologiya i venerologiya.2015; 14(2): 4. CrossRef Genetic regulation of immunoglobulin E level in different pathological states: integration of mouse and human genetics Elena S. Gusareva, Iryna Kurey, Igor Grekov, Marie Lipoldová Biological Reviews.2014; 89(2): 375. CrossRef Thymic stromal lymphopoietin and atopic diseases J.M. Leyva-Castillo, M. Li Revue Française d'Allergologie.2014; 54(5): 364. CrossRef Parasitic Nematode-Induced CD4+Foxp3+T Cells Can Ameliorate Allergic Airway Inflammation Shin Ae Kang, Mi-Kyung Park, Min Kyoung Cho, Sang Kyun Park, Min Seong Jang, Bo-Gie Yang, Myoung Ho Jang, Dong-Hee Kim, Hak Sun Yu, Edward Mitre PLoS Neglected Tropical Diseases.2014; 8(12): e3410. CrossRef Coinfection with Clonorchis sinensis modulates murine host response against Trichinella spiralis infection Ying Chen, Bo Huang, Shiguang Huang, Xinbing Yu, Yonglong Li, Wenjian Song, Yongxiang Li, Fangli Lu Parasitology Research.2013; 112(9): 3167. CrossRef Alteration of Cytokine Production during Visceral Larva Migrans by Toxascaris leonina in Mice Shin Ae Kang, Mi-Kyung Park, Min Kyoung Cho, Hak Sun Yu The Korean Journal of Parasitology.2013; 51(5): 583. CrossRef Toward Drugs for Protease-Activated Receptor 2 (PAR2) Mei-Kwan Yau, Ligong Liu, David P. Fairlie Journal of Medicinal Chemistry.2013; 56(19): 7477. CrossRef IL-32-PAR2 axis is an innate immunity sensor providing alternative signaling for LPS-TRIF axis Masanori Nakayama, Yasuo Niki, Toshiki Kawasaki, Yuki Takeda, Hiroyasu Ikegami, Yoshiaki Toyama, Takeshi Miyamoto Scientific Reports.2013;[Epub] CrossRef Protease‐activated receptor‐2 signalling by tissue factor on dendritic cells suppresses antigen‐specific CD4+ T‐cell priming Seema Shrivastava, Liang Ma, El‐Li Tham, John H. McVey, Daxin Chen, Anthony Dorling Immunology.2013; 139(2): 219. CrossRef Molecular characterization of 45kDa aspartic protease of Trichinella spiralis Jong Nam Park, Sang Kyun Park, Min Kyoung Cho, Mi-Kyung Park, Shin Ae Kang, Dong-Hee Kim, Hak Sun Yu Veterinary Parasitology.2012; 190(3-4): 510. CrossRef Regulation and dysregulation of innate immunity by NFAT signaling downstream of pattern recognition receptors (PRRs) Ivan Zanoni, Francesca Granucci European Journal of Immunology.2012; 42(8): 1924. CrossRef TSLP Expression: Cellular Sources, Triggers, and Regulatory Mechanisms Toshiro Takai Allergology International.2012; 61(1): 3. CrossRef Regulation of epithelial immunity by IL-17 family cytokines Rajita Pappu, Sascha Rutz, Wenjun Ouyang Trends in Immunology.2012; 33(7): 343. CrossRef Regulation of type 2 immunity to helminths by mast cells Matthew R. Hepworth, Marcus Maurer, Susanne Hartmann Gut Microbes.2012; 3(5): 476. CrossRef 10,737 View 86 Download Crossref Parasitic Helminth Cystatin Inhibits DSS-Induced Intestinal Inflammation Via IL-10⁺F4/80⁺ Macrophage Recruitment Sung Won Jang, Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Byoung-Kuk Na, Soon Cheol Ahn, Dong-Hee Kim, Hak Sun Yu Korean J Parasitol 2011;49(3):245-254. Published online September 30, 2011 DOI: https://doi.org/10.3347/kjp.2011.49.3.245 Abstract PDF Many immune down-regulatory molecules have been isolated from parasites, including cystatin (cystain protease inhibitor). In a previous study, we isolated and characterized Type I cystatin (CsStefin-1) of the liver fluke, Clonorchis sinensis. To investigate whether the CsStefin-1 might be a new host immune modulator, we induced intestinal inflammation in mice by dextran sodium sulfate (DSS) and treated them with recombinant CsStefin-1 (rCsStefin-1). The disease activity index (DAI) increased in DSS only-treated mice. In contrast, the DAI value was significantly reduced in rCsStefin-1-treated mice than DSS only-treated mice. In addition, the colon length of DSS only-treated mice was shorter than that of rCsStefin-1 treated mice. The secretion levels of IFN-γ and TNF-α in the spleen and mesenteric lymph nodes (MLNs) were significantly increased by DSS treatment, but the level of TNF-α in MLNs was significantly decreased by rCsStefin-1 treatment. IL-10 production in both spleen and MLNs was significantly increased, and IL-10⁺F4/80⁺ macrophage cells were significantly increased in the spleen and MLNs of rCsStefin-1 treated mice after DSS treatment. In conclusion, rCsStefin-1 could reduce the intestinal inflammation occurring after DSS treatment, these effects might be related with recruitment of IL-10 secreting macrophages. Citations Citations to this article as recorded by Crystal structure of Echinococcus multilocularis cystatin B reveals a novel feature in classical stefins Wenbin Hong, Zhe Cheng, Zhijian Xu, Shukun Zhong, Xianshu Liu, Nouhoum Dibo, Ziyi Dai, Yuzhou Lin, Wenchang Lai, HuaRui Jia, Xiaomin Shang, Shuaiqin Huang Scientific Reports.2026;[Epub] CrossRef Schistosoma japonicum cystatin attenuated CLP-induced sepsis in mice though inducing tolerogenic dendritic cells and regulatory T cells Yi-Xiang Wang, Yi-Dan Ma, Hui-Hui Li, Wen-Juan Duo, Qi-Wang Jin, Kai-Jun Zhou, Yan-Ran Gao, Jun-Nan He, Yu-Jie Xie, Liang Chu, Xiao-Di Yang Comparative Immunology, Microbiology and Infectious Diseases.2025; 120: 102345. 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Published online December 1, 2009 DOI: https://doi.org/10.3347/kjp.2009.47.4.337 Abstract PDF In a previous study, we reported our discovery of Acanthamoeba contamination in domestic tap water; in that study, we determined that some Acanthamoeba strains harbor endosymbiotic bacteria, via our molecular characterization by mitochondrial DNA restriction fragment length polymorphism (Mt DNA RFLP). Five (29.4%) among 17 Acanthamoeba isolates contained endosymbionts in their cytoplasm, as demonstrated via orcein staining. In order to estimate their pathogenicity, we conducted a genetic characterization of the endosymbionts in Acanthamoeba isolated from domestic tap water via 16S rDNA sequencing. The endosymbionts of Acanthamoeba sp. KA/WP3 and KA/WP4 evidenced the highest level of similarity, at 97% of the recently published 16S rDNA sequence of the bacterium, Candidatus Amoebophilus asiaticus. The endosymbionts of Acanthamoeba sp. KA/WP8 and KA/WP12 shared a 97% sequence similarity with each other, and were also highly similar to Candidatus Odyssella thessalonicensis, a member of the α-proteobacteria. The endosymbiont of Acanthamoeba sp. KA/WP9 exhibits a high degree of similarity (85-95%) with genus Methylophilus, which is not yet known to harbor any endosymbionts. This is the first report, to the best of our knowledge, to show that Methylophilus spp. can live in the cytoplasm of Acanthamoeba. Citations Citations to this article as recorded by An Evolutionary-Focused Review of the Holosporales (Alphaproteobacteria): Diversity, Host Interactions, and Taxonomic Re-ranking as Holosporineae Subord. 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