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Sudchit Chungpivat	2 Articles
Rapid Detection and Identification of Wuchereria bancrofti, Brugia malayi, B. pahangi, and Dirofilaria immitis in Mosquito Vectors and Blood Samples by High Resolution Melting Real-Time P Tongjit Thanchomnang, Pewpan M. Intapan, Chairat Tantrawatpan, Viraphong Lulitanond, Sudchit Chungpivat, Piyanan Taweethavonsawat, Worasak Kaewkong, Oranuch Sanpool, Penchom Janwan, Wej Choochote, Wanchai Maleewong Korean J Parasitol 2013;51(6):645-650. Published online December 31, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.6.645 Abstract PDF A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at 81.5±0.2℃, 79.0±0.3℃, 76.8±0.1℃, and 79.9±0.1℃, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors. Citations Citations to this article as recorded by Beyond Tradition: Exploring Cutting-Edge Approaches for Accurate Diagnosis of Human Filariasis Damian Pietrzak, Julia Weronika Łuczak, Marcin Wiśniewski Pathogens.2024; 13(6): 447. CrossRef Detecting Dirofilaria immitis: Current Practices and Novel Diagnostic Methods Damian Pietrzak, Julia Weronika Łuczak, Marcin Wiśniewski Pathogens.2024; 13(11): 950. CrossRef IDENTIFICATION OF MICROFILARIAE USING CONVENTIONAL POLYMERASE CHAIN REACTION AND QPCR-HRM Bagus Muhammad Ihsan, Widyana Lakshmi Puspita, Linda Triana, Wahdaniah, Khayan, Cecep Dani Sucipto Journal of Vocational Health Studies.2024; 8(1): 42. CrossRef Mosquitoes, Lymphatic Filariasis, and Public Health: A Systematic Review of Anopheles and Aedes Surveillance Strategies Arumugam Bhuvaneswari, Ananganallur Nagarajan Shriram, Kishan Hari K. Raju, Ashwani Kumar Pathogens.2023; 12(12): 1406. CrossRef Microfilaria Positification Test Using Real-Time PCR Technique with HRM (High-Resolution Melting) Bagus Muhammad Ihsan, Afiat Berbudi, Ridad Agoes, Muhammad Ifham Hanif Acta Parasitologica.2022; 67(1): 496. CrossRef Collection and DNA Detection of Dirofilaria immitis (Rhabditida Onchocercidae), Using a Novel Primer Set, in Wild-Caught Mosquitoes From Gainesville, FL Chris Holderman, Nicole O Abruzzo, Noor A Abdelsamad, Phillip E Kaufman, Peter M DiGennaro, Stephen Rich Journal of Medical Entomology.2021; 58(3): 1429. CrossRef The use of molecular xenomonitoring for surveillance of mosquito-borne diseases Mary M. Cameron, Anita Ramesh Philosophical Transactions of the Royal Society B: Biological Sciences.2021; 376(1818): 20190816. CrossRef Canine and feline vector-borne diseases of zoonotic concern in Southeast Asia Viet-Linh Nguyen, Filipe Dantas-Torres, Domenico Otranto Current Research in Parasitology & Vector-Borne Diseases.2021; 1: 100001. CrossRef The Incidence of Dirofilaria immitis in Shelter Dogs and Mosquitoes in Austria Karin Sonnberger, Hans-Peter Fuehrer, Bernhard Werner Sonnberger, Michael Leschnik Pathogens.2021; 10(5): 550. CrossRef Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases Madeleine J. Rogers, Donald P. McManus, Stephen Muhi, Catherine A. Gordon Clinical Microbiology Reviews.2021;[Epub] CrossRef PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION Bagus Muhammad Ihsan, Cecep Dani Sucipto, Khayan Khayan Journal of Vocational Health Studies.2021; 5(1): 26. CrossRef Molecular detection of Trichostrongylus species through PCR followed by high resolution melt analysis of ITS-2 rDNA sequences Mohsen Arbabi, Hossein Hooshyar, Majid Lotfinia, Mohamad Ali Bakhshi Molecular and Biochemical Parasitology.2020; 236: 111260. CrossRef Prevalence and risk factors associated with Dirofilaria immitis infection in dogs and cats in Songkhla and Satun provinces, Thailand Ketsarin Kamyingkird, Witchuta Junsiri, Witsanuwat Chimnoi, Chanya Kengradomkij, Sinsamut Saengow, Keplee Sangchuto, Wicha Kajeerum, Decha Pangjai, Burin Nimsuphan, Tawin Inpankeaw, Sathaporn Jittapalapong Agriculture and Natural Resources.2017; 51(4): 299. CrossRef Is molecular xenomonitoring of mosquitoes for Dirofilaria repens suitable for dirofilariosis surveillance in endemic regions? Aleksander Masny, Rusłan Sałamatin, Wioletta Rozej-Bielicka, Elzbieta Golab Parasitology Research.2016; 115(2): 511. CrossRef Pyrosequencing Using SL and 5S rRNA as Molecular Markers for Identifying Zoonotic Filarial Nematodes in Blood Samples and Mosquitoes Oranuch Sanpool, Chairat Tantrawatpan, Tongjit Thanchomnang, Penchom Janwan, Pewpan M. Intapan, Rutchanee Rodpai, Viraphong Lulitanond, Piyanan Taweethavonsawat, Wanchai Maleewong Vector-Borne and Zoonotic Diseases.2016; 16(5): 326. CrossRef Detection and quantification of Wuchereria bancrofti and Brugia malayi DNA in blood samples and mosquitoes using duplex droplet digital polymerase chain reaction Jurairat Jongthawin, Pewpan M. Intapan, Viraphong Lulitanond, Oranuch Sanpool, Tongjit Thanchomnang, Lakkhana Sadaow, Wanchai Maleewong Parasitology Research.2016; 115(8): 2967. CrossRef Trypanosome infection rates in tsetse flies in the “silent” sleeping sickness focus of Bafia in the Centre Region in Cameroon Gustave Simo, Pierre Fongho, Oumarou Farikou, Prosper Innocent Ndjeuto Ndjeuto-Tchouli, Judith Tchouomene-Labou, Flobert Njiokou, Tazoacha Asonganyi Parasites & Vectors.2015;[Epub] CrossRef 11,856 View 137 Download Crossref Successful Treatment of Brugia pahangi in Naturally Infected Cats with Ivermectin Piyanan Taweethavonsawat, Sudchit Chungpivat Korean J Parasitol 2013;51(6):759-761. Published online December 31, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.6.759 Abstract PDF Lymphatic filariasis is a common parasitic disease of cats in tropical regions including Thailand. The objective of this study was to determine the efficacy of ivermectin against microfilariae of Brugia pahangi in naturally infected cats. Eight cats naturally infected with B. pahangi were divided into control (untreated) and treated groups. Cats in the latter group were given ivermectin injection at 400 ?g/kg weekly for 2 months. Microfilariae were counted every week until 48 weeks. Microfilaremia was significantly decreased in the treated group 4 weeks after starting the treatment and become zero at week 9 and afterwards. On the other hand, cats in the control group had high microfilaremia throughout the study. It was successful to treat and control B. pahangi infection in naturally infected cats using ivermectin. Citations Citations to this article as recorded by Filariasis of the breast caused by Brugia pahangi: A concomitant finding with invasive ductal carcinoma Jerapas Thongpiya, Doonyapat Sa-nguanraksa, Norasate Samarnthai, Patsharaporn T. Sarasombath Parasitology International.2021; 80: 102203. CrossRef Comparison of efficacy of ivermectin and diethylcarbamazine against naturally infected Brugia malayi microfilaria in dogs Poojary Vineeta Sadarama, Deepa Chirayath, Usha Narayana Pillai, Bindu Lakshmanan Journal of Parasitic Diseases.2019; 43(4): 554. CrossRef Therapeutic trial of doxycyclin plus ivermectin for the treatment of Brugia malayi naturally infected cats Ladawan Khowawisetsut, Patsharaporn T. Sarasombath, Suwich Thammapalo, Sumart Loymek, Therayot Korbarsa, Hathai Nochote, Achinya Phuakrod, Wej Choochote, Sirichit Wongkamchai Veterinary Parasitology.2017; 245: 42. CrossRef 7,840 View 88 Download Crossref