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Suher Zada	2 Articles
T Regulatory Cell Responses to Immunization with a Soluble Egg Antigen in Schistosoma mansoni-Infected Mice Eman El-Ahwany, Ibrahim Rabia Bauiomy, Faten Nagy, Rabab Zalat, Ola Mahmoud, Suher Zada Korean J Parasitol 2012;50(1):29-35. Published online March 6, 2012 DOI: https://doi.org/10.3347/kjp.2012.50.1.29 Abstract PDF The aim of the study is to characterize the phenotypes of CD4⁺ CD25⁺ T regulatory cells within the liver granulomas and association with both Foxp-3 gene expression and splenic cytokines. Na?ve C57BL/6 mice were intravenously injected with multiple doses of the soluble egg antigen (SEA) 7 days before cercarial infection. The immunized and infected control groups were sacrificed 8 and 16 weeks post-infection (PI). Histopathology, parasitological parameters, splenic phenotypes for T regulatory cells, the FOXP-3 expression in hepatic granuloma using real-time PCR, and the associated splenic cytokines were studied. Histopathological examination of the liver revealed remarkable increase in degenerated ova within hepatic granuloma which decreased in diameter at weeks 8 and 16 PI (P<0.01). The percentage of T regulatory cells (CD4⁺ CD25⁺) increased significantly (P<0.01) in the immunized group compared to the infected control at weeks 8 and 16 PI. The FOXP-3 expression in hepatic granulomas increased from 10 at week 8 to 30 fold at week 16 PI in the infected control group. However, its expression in the immunized group showed an increase from 30 at week 8 to 70 fold at week 16 PI. The splenic cytokine levels of pro-inflammatory cytokines, IFN-γ, IL-4, and TNF-α, showed significant decreases (P<0.05) compared to the infected control group. In conclusion, the magnitude and phenotype of the egg-induced effects on T helper responses were found to be controlled by a parallel response within the T regulatory population which provides protection in worm parasite-induced immunopathology. Citations Citations to this article as recorded by Micromorphological Changes in the Parenchymatous Organs of Muskrats Infected with Quinqueserialis quinqueserialis (Trematoda: Notocotilidae) O. E. Mazur, A. S. Fomina Biology Bulletin.2024; 51(2): 371. CrossRef *Micromorphological changes in the parenchymatous organs of the muskrat infected with Quinqueserialis quinqueserialis* (Trematoda: Notocotilidae)** O. E. Mazur, A. S. Fomina Известия Российской академии наук. Серия биологическая.2024;[Epub] CrossRef Evaluation of schistosomula lung antigen preparation and soluble egg antigen vaccines on experimental schistosomiasis mansoni Nagwa S. M. Aly, Hye-Sook Kim, Maysa A. Eraky, Asmaa A. El Kholy, Basma T. Ali, Shin-ichi Miyoshi, Rabab E. Omar Parasites, Hosts and Diseases.2023; 61(3): 251. CrossRef Protective effect and mechanism of Schistosoma japonicum soluble egg antigen against type 1 diabetes in NOD mice Li-xia Wang, Yan-ru Gao, Qun Pan, Chun-lian Tang, Rong-hui Zhang, Yan-hong Li, Chun-lan Zheng International Journal of Diabetes in Developing Countries.2022; 42(2): 363. CrossRef Schistosoma japonicum Infection in Treg-Specific USP21 Knockout Mice Youxiang Zhang, De-Hui Xiong, Yangyang Li, Guina Xu, Baoxin Zhang, Yang Liu, Shan Zhang, Qing Huang, Simin Chen, Fansheng Zeng, Jingyi Guo, Bin Li, Zhiqiang Qin, Zuping Zhang, Luiz Felipe Domingues Passero Journal of Immunology Research.2021; 2021: 1. CrossRef Role of regulatory T cells in Schistosoma-mediated protection against type 1 diabetes Chun-lian Tang, Yan-ru Gao, Li-xia Wang, Ya-wen Zhu, Qun Pan, Rong-hui Zhang, Ying Xiong Molecular and Cellular Endocrinology.2019; 491: 110434. CrossRef Schistosoma japonicum Soluble Egg Antigen Protects Against Type 2 Diabetes in Leprdb/db Mice by Enhancing Regulatory T Cells and Th2 Cytokines Chun-lian Tang, Xiao-hong Yu, Yan Li, Rong-hui Zhang, Jun Xie, Zhi-ming Liu Frontiers in Immunology.2019;[Epub] CrossRef Maternal schistosomiasis: IL-2, IL-10 and regulatory T lymphocytes to unrelated antigen in adult offspring mice Erica de Souza Fernandes, Virgínia Maria de Barros Lorena, Iana Rafaela Fernandes Sales, Mônica Camelo Pessoa de Azevedo Albuquerque, Yara de Miranda Gomes, Vlaudia Maria Assis Costa, Valdênia Maria Oliveira de Souza Revista da Sociedade Brasileira de Medicina Tropical.2018; 51(4): 546. CrossRef Heat Shock Protein 60 in Eggs Specifically Induces Tregs and Reduces Liver Immunopathology in Mice with Schistosomiasis Japonica Sha Zhou, Xin Jin, Xiaojun Chen, Jifeng Zhu, Zhipeng Xu, Xuefeng Wang, Feng Liu, Wei Hu, Liang Zhou, Chuan Su, Susmit Suvas PLOS ONE.2015; 10(9): e0139133. CrossRef Morphological features of cellular responses to different rates of trematode Quinqueserialis quinqueserialis (Trematoda: Notocotilidae) invasion in muskrat (Ondatra zibethicus) O. E. Mazur, A. S. Fomina Biology Bulletin.2014; 41(5): 444. CrossRef 9,505 View 94 Download Crossref Protective Role of Purified Cysteine Proteinases against Fasciola gigantica Infection in Experimental Animals Eman EL-Ahwany, Ibrahim Rabia, Faten Nagy, Mona Zoheiry, Tarek Diab, Suher Zada Korean J Parasitol 2012;50(1):45-51. Published online March 6, 2012 DOI: https://doi.org/10.3347/kjp.2012.50.1.45 Abstract PDF Fascioliasis is one of the public health problems in the world. Cysteine proteinases (CP) released by Fasciola gigantica play a key role in parasite feeding, migration through host tissues, and in immune evasion. There has been some evidence from several parasite systems that proteinases might have potential as protective antigens against parasitic infections. Cysteine proteinases were purified and tested in vaccine trials of sheep infected with the liver fluke. Multiple doses (2 mg of CP in Freund's adjuvant followed by 3 booster doses 1 mg each at 4 week intervals) were injected intramuscularly into sheep 1 week prior to infect orally with 300 F. gigantica metacercariae. All the sheep were humanely slaughtered 12 weeks after the first immunization. Changes in the worm burden, ova count, and humoral and cellular responses were evaluated. Significant reduction was observed in the worm burden (56.9%), bile egg count (70.7%), and fecel egg count (75.2%). Immunization with CP was also found to be associated with increases of total IgG, IgG₁, and IgG₂ (P<0.05). Data showed that the serum cytokine levels of pro-inflammatory cytokines, IL-12, IFN-γ, and TNF-α, revealed significant decreases (P<0.05). However, the anti-inflammatory cytokine levels, IL-10, TGF-β, and IL-6, showed significant increases (P<0.05). In conclusion, it has been found that CP released by F. gigantica are highly important candidates for a vaccine antigen because of their role in the fluke biology and host-parasite relationships. Citations Citations to this article as recorded by Genetic diversity and adaptability of native sheep breeds from different climatic zones George Wanjala, Zoltán Bagi, Dinu Gavojdian, Bouabid Badaoui, Putri Kusuma Astuti, Alexandru Mizeranschi, Elena Ilisiu, Husein Ohran, Eva Pasic Juhas, Dimitrios Loukovitis, Aldona Kawęcka, Rūta Šveistienė, Zsolt Becskei, Péter Strausz, Nelly Kichamu, Szil Scientific Reports.2025;[Epub] CrossRef Serodiagnosis of Fasciola gigantica Infection in Buffaloes with Native Cathepsin-L Proteases and Recombinant Cathepsin L1-D Andleeb Aftab, Rohit Lall, Savita Bisen, Arun Anandanarayanan, Ajayta Rialch, Jayanta K. Chamuah, Shobha Yadav, M. Silamparasan, O. K. Raina Acta Parasitologica.2020; 65(2): 413. CrossRef A Survey on the Adjuvant Role of Naloxone Alone or Combined with Alum in Vaccination Against Fasciolosis in BALB/c Mice Hakim Azizi, Hadi Mirzaeei, Amin Bagheri, Ali Bazi, Ali Khamesipour, Hajar Yaghoobi, Aliyar Mirzapour, Mehrdad Khatami, Samira Elikaee Acta Parasitologica.2019;[Epub] CrossRef Oral delivery of Bacillus subtilis spores expressing cysteine protease of Clonorchis sinensis to grass carp ( Ctenopharyngodon idellus ): Induces immune responses and has no damage on liver and intestine function Zeli Tang, Hengchang Sun, TingJin Chen, Zhipeng Lin, Hongye Jiang, Xinyi Zhou, Cunbin Shi, Houjun Pan, Ouqin Chang, Pengli Ren, Jinyun Yu, Xuerong Li, Jin Xu, Yan Huang, Xinbing Yu Fish & Shellfish Immunology.2017; 64: 287. CrossRef Comparative analysis of immune effects in mice model: Clonorchis sinensis cysteine protease generated from recombinant Escherichia coli and Bacillus subtilis spores Zhanshuai Wu, Zeli Tang, Mei Shang, Lu Zhao, Lina Zhou, Xiangzhan Kong, Zhipeng Lin, Hengchang Sun, Tingjin Chen, Jin Xu, Xuerong Li, Yan Huang, Xinbing Yu Parasitology Research.2017; 116(7): 1811. CrossRef The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease Zeli Tang, Mei Shang, Tingjin Chen, Pengli Ren, Hengchang Sun, Hongling Qu, Zhipeng Lin, Lina Zhou, Jinyun Yu, Hongye Jiang, Xinyi Zhou, Xuerong Li, Yan Huang, Jin Xu, Xinbing Yu Parasites & Vectors.2016;[Epub] CrossRef Immunological features of LPS from Ochrobactrum intermedium on sheep experimentally infected with Fasciola hepatica J.M. Martínez-Pérez, D. Robles-Pérez, F.A. Rojo-Vázquez, M. Martínez-Valladares Research in Veterinary Science.2014; 97(2): 329. CrossRef Liver fluke vaccines in ruminants: strategies, progress and future opportunities Hayley Toet, David M. Piedrafita, Terry W. Spithill International Journal for Parasitology.2014; 44(12): 915. CrossRef 8,714 View 82 Download Crossref