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Sun Mi Kim	3 Articles
Expression and Characterization of α-Methylacyl CoA Racemase from Anisakis simplex Larvae Bong Jin Kim, Sun Mi Kim, Min Kyung Cho, Hak Sun Yu, Yong Seok Lee, Hee Jae Cha, Meesun Ock Korean J Parasitol 2012;50(2):165-171. Published online May 24, 2012 DOI: https://doi.org/10.3347/kjp.2012.50.2.165 Abstract PDF Larval excretory-secretory products of Anisakis simplex are known to cause allergic reactions in humans. A cDNA library of A. simplex 3rd-stage larvae (L3) was immunoscreened with polyclonal rabbit serum raised against A. simplex L3 excretory-secretory products to identify an antigen that elicits the immune response. One cDNA clone, designated as α-methylacyl CoA racemase (Amacr) contained a 1,412 bp cDNA transcript with a single open reading frame that encoded 418 amino acids. A. simplex Amacr showed a high degree of homology compared to Amacr orthologs from other species. Amacr mRNA was highly and constitutively expressed regardless of temperature (10-40℃) and time (24-48 hr). Immunohistochemical analysis revealed that Amacr was expressed mainly in the ventriculus of A. simplex larvae. The Amacr protein produced in large quantities from the ventriculus is probably responsible for many functions in the development and growth of A. simplex larvae. Citations Citations to this article as recorded by The mitochondrial genome ofAncylostoma tubaeformefrom cats in China X.L. Shi, Y.Q. Fu, A.Y. Abdullahi, M.W. Wang, F. Yang, X.G. Yu, W.D. Pan, X.X. Yan, J.X. Hang, P. Zhang, G.Q. Li Journal of Helminthology.2018; 92(1): 22. CrossRef α-Methylacyl-CoA racemase (AMACR): Metabolic enzyme, drug metabolizer and cancer marker P504S Matthew D. Lloyd, Maksims Yevglevskis, Guat Ling Lee, Pauline J. Wood, Michael D. Threadgill, Timothy J. Woodman Progress in Lipid Research.2013; 52(2): 220. CrossRef 10,030 View 51 Download Crossref Expressed sequence tags analysis of Blattella germanica Hyang Suk Chung, Tai Hyun Yu, Bong Jin Kim, Sun Mi Kim, Joo Yeong Kim, Hak Sun Yu, Hae Jin Jeong, Mee Sun Ock Korean J Parasitol 2005;43(4):149-156. Published online December 20, 2005 DOI: https://doi.org/10.3347/kjp.2005.43.4.149 Abstract PDF Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these represent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome. Citations Citations to this article as recorded by The Distribution Characteristics of Microsatellites in the Transcriptome of Periplaneta americana Lifang Zhang, Shangeng He Cytology and Genetics.2023; 57(5): 491. CrossRef Identification and characterization of microRNAs in American cockroach (Periplaneta americana) Qiao Yang, Zheng Bao, Mingyu Yang, Yongmei Shen, Xiuyue Zhang, Bisong Yue, Yang Meng, Zhenxin Fan Gene.2020; 743: 144610. CrossRef New insight into foregut functions of xenobiotic detoxification in the cockroach Periplaneta americana Jian‐Hua Zhang, Song Zhang, Yuan‐Xue Yang, Yi‐Xi Zhang, Ze‐Wen Liu Insect Science.2018; 25(6): 978. CrossRef Cockroach allergen serine proteinases: Isolation, sequencing and signalling via proteinase‐activated receptor‐2 D. J. Polley, K. Mihara, R. Ramachandran, H. Vliagoftis, B. Renaux, M. Saifeddine, M. O. Daines, S. Boitano, M. D. Hollenberg Clinical & Experimental Allergy.2017; 47(7): 946. CrossRef De Novo Transcriptome Analysis and Detection of Antimicrobial Peptides of the American Cockroach Periplaneta americana (Linnaeus) In-Woo Kim, Joon Ha Lee, Sathiyamoorthy Subramaniyam, Eun-Young Yun, Iksoo Kim, Junhyung Park, Jae Sam Hwang, Surajit Bhattacharjya PLOS ONE.2016; 11(5): e0155304. CrossRef Midgut Transcriptome of the Cockroach Periplaneta americana and Its Microbiota: Digestion, Detoxification and Oxidative Stress Response Jianhua Zhang, Yixi Zhang, Jingjing Li, Meiling Liu, Zewen Liu, Xinghui Qiu PLOS ONE.2016; 11(5): e0155254. CrossRef Analysis of German cockroach (Blattella germanica) expressed sequence tags Ji Eun Jeong, Hee Ju Hwang, Hong Seog Park, Hee Jae Cha, Yong Seok Lee, Meesun Ock Genes & Genomics.2015; 37(3): 271. CrossRef Allergenic Characterization of a Novel Allergen, Homologous to Chymotrypsin, from German Cockroach Kyoung Yong Jeong, Mina Son, Jae-Hyun Lee, Chein-Soo Hong, Jung-Won Park Allergy, Asthma & Immunology Research.2015; 7(3): 283. CrossRef De novo Assembly and Characterization of the Testis Transcriptome and Development of EST-SSR Markers in the Cockroach Periplaneta americana Wan Chen, Yu-Xiang Liu, Guo-Fang Jiang Scientific Reports.2015;[Epub] CrossRef De Novo Transcriptome of the Hemimetabolous German Cockroach (Blattella germanica) Xiaojie Zhou, Kun Qian, Ying Tong, Junwei Jerry Zhu, Xinghui Qiu, Xiaopeng Zeng, Yara M. Traub-Csekö PLoS ONE.2014; 9(9): e106932. CrossRef Identification of differentially expressed genes in American cockroach ovaries and testes by suppression subtractive hybridization and the prediction of its miRNAs Wan Chen, Guo-Fang Jiang, Shu-Hong Sun, Yong Lu, Fei Ma, Bin Li Molecular Genetics and Genomics.2013; 288(11): 627. CrossRef 9,829 View 78 Download Crossref Cloning and expression of trypsin-encoding cDNA from Blattella germanica and its possibility as an allergen Mee Sun Ock, Bong Jin Kim, Sun Mi Kim, Kang Hyun Byun Korean J Parasitol 2005;43(3):101-110. Published online September 20, 2005 DOI: https://doi.org/10.3347/kjp.2005.43.3.101 Abstract PDF In this study, the trypsin gene (bgtryp-1) from the German cockroach, Blattella germanica, was cloned via the immunoscreening of patients with allergies to cockroaches. Nucleotide sequence analysis predicted an 863 bp open reading frame which encodes for 257 amino acids. The deduced amino acid sequence exhibited 42-57% homology with the serine protease from dust mites, and consisted of a conserved catalytic domain (GDSGGPLV). bgtryp-1 was determined by both Northern and Southern analysis to be a 0.9 kb, single-copy gene. SDS-PAGE and Western blotting analyses of the recombinant protein (Bgtryp-1) over-expressed in Escherichia coli revealed that the molecular mass of the expressed protein was 35 kDa, and the expressed protein was capable of reacting with the sera of cockroach allergy patients. We also discussed the possibility that trypsin excreted by the digestive system of the German cockroach not only functions as an allergen, but also may perform a vital role in the activation of PAR-2. Citations Citations to this article as recorded by Proteinase‐activated receptor‐2 antagonist C391 inhibits Alternaria‐induced airway epithelial signalling and asthma indicators in acute exposure mouse models Candy M. Rivas, Michael C. Yee, Kenneth J. Addison, Marissa Lovett, Kasturi Pal, Julie G. Ledford, Gregory Dussor, Theodore J. Price, Josef Vagner, Kathryn A. DeFea, Scott Boitano British Journal of Pharmacology.2022; 179(10): 2208. CrossRef Insect Allergens on the Dining Table Kyoung Yong Jeong, Jung-Won Park Current Protein & Peptide Science.2020; 21(2): 159. CrossRef Protease-activated receptor-2 signaling through β-arrestin-2 mediates Alternaria alkaline serine protease-induced airway inflammation Michael C. Yee, Heddie L. Nichols, Danny Polley, Mahmoud Saifeddine, Kasturi Pal, Kyu Lee, Emma H. Wilson, Michael O. Daines, Morley D. Hollenberg, Scott Boitano, Kathryn A. DeFea American Journal of Physiology-Lung Cellular and Molecular Physiology.2018; 315(6): L1042. CrossRef Cockroach allergen serine proteinases: Isolation, sequencing and signalling via proteinase‐activated receptor‐2 D. J. Polley, K. Mihara, R. Ramachandran, H. Vliagoftis, B. Renaux, M. Saifeddine, M. O. Daines, S. Boitano, M. D. Hollenberg Clinical & Experimental Allergy.2017; 47(7): 946. CrossRef Allergenic Characterization of a Novel Allergen, Homologous to Chymotrypsin, from German Cockroach Kyoung Yong Jeong, Mina Son, Jae-Hyun Lee, Chein-Soo Hong, Jung-Won Park Allergy, Asthma & Immunology Research.2015; 7(3): 283. CrossRef Characterization of Recombinant Per a 10 from Periplaneta americana Dhanapal Govindaraj, Shailendra Nath Gaur, Naveen Arora Clinical and Vaccine Immunology.2013; 20(2): 262. CrossRef Characterization of a chemostable serine alkaline protease from Periplaneta americana Prashant T Sanatan, Purushottam R Lomate, Ashok P Giri, Vandana K Hivrale BMC Biochemistry.2013;[Epub] CrossRef Household Arthropod Allergens in Korea Tai-Soon Yong, Kyoung Yong Jeong The Korean Journal of Parasitology.2009; 47(Suppl): S143. CrossRef Identification of a serine protease as a major allergen (Per a 10) of Periplaneta americana V. T. Sudha, N. Arora, S. N. Gaur, S. Pasha, B. P. Singh Allergy.2008; 63(6): 768. CrossRef Inflammatory effect of environmental proteases on airway mucosa Charles E. Reed Current Allergy and Asthma Reports.2007; 7(5): 368. CrossRef Expressed sequence tags analysis of Blattella germanica Hyang Suk Chung, Tai Hyun Yu, Bong Jin Kim, Sun Mi Kim, Joo Yeong Kim, Hak Sun Yu, Hae Jin Jeong, Mee Sun Ock The Korean Journal of Parasitology.2005; 43(4): 149. CrossRef 10,143 View 66 Download Crossref