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Sung Won Jang	1 Article
Parasitic Helminth Cystatin Inhibits DSS-Induced Intestinal Inflammation Via IL-10⁺F4/80⁺ Macrophage Recruitment Sung Won Jang, Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Byoung-Kuk Na, Soon Cheol Ahn, Dong-Hee Kim, Hak Sun Yu Korean J Parasitol 2011;49(3):245-254. Published online September 30, 2011 DOI: https://doi.org/10.3347/kjp.2011.49.3.245 Abstract PDF Many immune down-regulatory molecules have been isolated from parasites, including cystatin (cystain protease inhibitor). In a previous study, we isolated and characterized Type I cystatin (CsStefin-1) of the liver fluke, Clonorchis sinensis. To investigate whether the CsStefin-1 might be a new host immune modulator, we induced intestinal inflammation in mice by dextran sodium sulfate (DSS) and treated them with recombinant CsStefin-1 (rCsStefin-1). The disease activity index (DAI) increased in DSS only-treated mice. In contrast, the DAI value was significantly reduced in rCsStefin-1-treated mice than DSS only-treated mice. In addition, the colon length of DSS only-treated mice was shorter than that of rCsStefin-1 treated mice. The secretion levels of IFN-γ and TNF-α in the spleen and mesenteric lymph nodes (MLNs) were significantly increased by DSS treatment, but the level of TNF-α in MLNs was significantly decreased by rCsStefin-1 treatment. IL-10 production in both spleen and MLNs was significantly increased, and IL-10⁺F4/80⁺ macrophage cells were significantly increased in the spleen and MLNs of rCsStefin-1 treated mice after DSS treatment. In conclusion, rCsStefin-1 could reduce the intestinal inflammation occurring after DSS treatment, these effects might be related with recruitment of IL-10 secreting macrophages. Citations Citations to this article as recorded by Crystal structure of Echinococcus multilocularis cystatin B reveals a novel feature in classical stefins Wenbin Hong, Zhe Cheng, Zhijian Xu, Shukun Zhong, Xianshu Liu, Nouhoum Dibo, Ziyi Dai, Yuzhou Lin, Wenchang Lai, HuaRui Jia, Xiaomin Shang, Shuaiqin Huang Scientific Reports.2026;[Epub] CrossRef Schistosoma japonicum cystatin attenuated CLP-induced sepsis in mice though inducing tolerogenic dendritic cells and regulatory T cells Yi-Xiang Wang, Yi-Dan Ma, Hui-Hui Li, Wen-Juan Duo, Qi-Wang Jin, Kai-Jun Zhou, Yan-Ran Gao, Jun-Nan He, Yu-Jie Xie, Liang Chu, Xiao-Di Yang Comparative Immunology, Microbiology and Infectious Diseases.2025; 120: 102345. 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