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Sung-Don Choi	2 Articles
The effect of microfilament inhibitor on the Cryptosporidium infection in vitro Jae-Ran Yu, Sung-Don Choi Korean J Parasitol 2000;38(4):257-261. Published online December 31, 2000 DOI: https://doi.org/10.3347/kjp.2000.38.4.257 Abstract PDF This study was focused on the effects of microfilament inhibitor, Cytochalasin D (CD) on the invasiveness of sporozoites of Cryptosporidium spp. into the host cells. MDCK and AGS cell lines were used as host cells for C. parvum and C. muris, respectively. When MDCK cells were pretreated with CD for 1 hr before inoculation of the sporozoites, C. parvum infection was significantly inhibited when compared to the control cells. These inhibitory effects of CD on the rate of infection were dose-dependent. In addition, C. muris infection was hampered when AGS cell lines were pretreated with CD. However, the capability of invasiveness of the sporozoites into the host cells was not greatly influenced by the pretreatment of sporozoites with CD before infection. These results suggest that microfilaments of host cells, rather than parasites, play an important role for the invasion of Cryptosporidium spp. Citations Citations to this article as recorded by Transcriptome analysis of pig intestinal cell monolayers infected with Cryptosporidium parvum asexual stages Marzieh Ezzaty Mirhashemi, Farzad Noubary, Susan Chapman-Bonofiglio, Saul Tzipori, Gordon S. Huggins, Giovanni Widmer Parasites & Vectors.2018;[Epub] CrossRef Giardia and Cryptosporidium and public health: the epidemiological scenario from the Italian perspective Annunziata Giangaspero, Federica Berrilli, Olga Brandonisio Parasitology Research.2007; 101(5): 1169. CrossRef Cryptosporidium parvum regulation of human epithelial cell gene expression Mingqi Deng, Cheryl A Lancto, Mitchell S Abrahamsen International Journal for Parasitology.2004; 34(1): 73. CrossRef In vitro culture of Cryptosporidium muris in a human stomach adenocarcinoma cell line Min-Ho Choi, Sung-Tae Hong, Jong-Yil Chai, Woo-Yoon Park, Jae-Ran Yu The Korean Journal of Parasitology.2004; 42(1): 27. CrossRef 7,893 View 67 Download Crossref In vitro infection of Cryptosporidium parvum to four different cell lines Jae-Ran Yu, Sung-Don Choi, Young-Wook Kim Korean J Parasitol 2000;38(2):59-64. Published online June 30, 2000 DOI: https://doi.org/10.3347/kjp.2000.38.2.59 Abstract PDF To determine a suitable condition for in vitro infection model of Cryptosporidium parvum, four different cell lines, AGS, MDCK, HCT-8 and Caco-2, were used as host cell lines which were cultured at various concentrations of added supplements. These supplement include fetal bovine serum (FBS), sodium choleate, ascorbic acid, folic acid, calcium pantothenate, para-aminobenzoic acid and pyruvate and their effects on the cell lines which were infected with C. parvum were evaluated. The results of this study showed that the AGS cell line was most susceptible to C. parvum whereas the Caco-2 cells appeared to be least susceptible to C. parvum. In regards to the serum condition, 10% FBS was suitable for the growth of AGS and HCT-8 cells, and 1% FBS was good for the growth of the MDCK cells when they were inoculated with C. parvum. Vitamines had a positive effect on the AGS cells, and pyruvate also showed positive effects on all of the cell lines except for Caco-2. Modified medium for each cell line was prepared by adding appropriate amounts of each supplement which resulted in the highest parasite infection number. Modified media increased the number of parasites infected on AGS cells to 2.3-fold higher when compared to the control media. In this study, we found that the AGS cell line was a suitable host model for evaluating C. parvum in vitro study and the media contents for the optimal infection conditions were suggested. Citations Citations to this article as recorded by Novel organoids and ex vivo models for advancing poultry coccidiosis research Phoebe Yuen Ka Chan, Bernat Marti-Garcia, Virginia Marugan-Hernandez Parasitology.2025; 152(11): 1094. CrossRef In vitro and in vivo anti-Cryptosporidium and anti-inflammatory effects of Aloe vera gel in dexamethasone immunosuppressed mice Alyaa Farid, Aya Tawfik, Basil Elsioufy, Gehan Safwat International Journal for Parasitology: Drugs and Drug Resistance.2021; 17: 156. CrossRef Response of cell lines to actual and simulated inoculation with Cryptosporidium proliferans Janka Melicherová, Lada Hofmannová, Andrea Valigurová European Journal of Protistology.2018; 62: 101. CrossRef A new in vitro model using small intestinal epithelial cells to enhance infection of Cryptosporidium parvum Eunice A. Varughese, Christina L. Bennett-Stamper, Larry J. Wymer, Jagjit S. Yadav Journal of Microbiological Methods.2014; 106: 47. CrossRef Apicomplexan infections in the gut C. L. Wilhelm, F. Yarovinsky Parasite Immunology.2014; 36(9): 409. CrossRef Evolution of Cryptosporidium in vitro culture P. Karanis, H.M. Aldeyarbi International Journal for Parasitology.2011; 41(12): 1231. CrossRef Morphology and infectivity of virus that persistently caused infection in an AGS cell line Yukimasa Ooi, Eriko Daikoku, Hong Wu, Hiroaki Aoki, Chizuko Morita, Takashi Nakano, Takehiro Kohno, Tomohiko Takasaki, Kouichi Sano Medical Molecular Morphology.2011; 44(4): 213. CrossRef Determining GFP Activity in HCT-8 Cells Infected withCryptosporidium Parvumby Reverse Transcription Polymerase Chain Reaction and Nested PCR Z. Koloren Biotechnology & Biotechnological Equipment.2010; 24(3): 2009. CrossRef Transient expression of red and yellow fluorescent protein vectors in HCT-8 cells ınfected with Cryptosporidium parvum Zeynep Koloren, Sadik Dinçer Parasitology Research.2009; 105(4): 1023. CrossRef In vitro development of Cryptosporidium parvum in serum-free media K.M. Woods, S.J. Upton Letters in Applied Microbiology.2007; 44(5): 520. CrossRef Cryptosporidium p30, a Galactose/N-Acetylgalactosamine-specific Lectin, Mediates Infection in Vitro Najma Bhat, Angela Joe, Mercio PereiraPerrin, Honorine D. Ward Journal of Biological Chemistry.2007; 282(48): 34877. CrossRef In vitro culture of Cryptosporidium muris in a human stomach adenocarcinoma cell line Min-Ho Choi, Sung-Tae Hong, Jong-Yil Chai, Woo-Yoon Park, Jae-Ran Yu The Korean Journal of Parasitology.2004; 42(1): 27. CrossRef MICROBIAL ADHESION OF CRYPTOSPORIDIUM PARVUM SPOROZOITES: PURIFICATION OF AN INHIBITORY LIPID FROM BOVINE MUCOSA Julie K. Johnson, Joann Schmidt, Howard B. Gelberg, Mark S. Kuhlenschmidt Journal of Parasitology.2004; 90(5): 980. CrossRef IcsB, secreted via the type III secretion system, is chaperoned by IpgA and required at the post‐invasion stage of Shigella pathogenicity Michinaga Ogawa, Toshihiko Suzuki, Ichiro Tatsuno, Hiroyuki Abe, Chihiro Sasakawa Molecular Microbiology.2003; 48(4): 913. CrossRef The effect of microfilament inhibitor on the Cryptosporidium infection in vitro Jae-Ran Yu, Sung-Don Choi The Korean Journal of Parasitology.2000; 38(4): 257. CrossRef 9,411 View 80 Download Crossref