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Sussan K. Ardestani	3 Articles
Purification and biochemical characterization of two novel antigens from Leishmania major promastigotes Majid Zeinali, Sussan K. Ardestani, Amina Kariminia Korean J Parasitol 2007;45(4):287-293. Published online December 20, 2007 DOI: https://doi.org/10.3347/kjp.2007.45.4.287 Abstract PDF The identification and characterization of antigens that elicit human T cell responses is an important step toward understanding of Leishmania major infection and ultimately in the development of a vaccine. Micropreparative SDS-PAGE followed by electrotransfer to a PVDF membrane and elution of proteins from the PVDF, was used to separate 2 novel proteins from L. major promastigotes, which can induce antibodies of the IgG2a isotype in mice and also are recognized by antisera of recovered human cutaneous leishmaniasis subjects. Fractionation of the crude extract of L. major revealed that all detectable proteins of interest were present within the soluble Leishmania antigens (SLA). Quantitation of these proteins showed that their expression in promastigotes is relatively very low. Considering the molecular weight, immunoreactivity, chromatographic and electrophoretic behavior in reducing and non-reducing conditions, these proteins are probably 2 isoforms of a single protein. A digest of these proteins was resolved on Tricine-SDS-PAGE and immunoreactive fragments were identified by human sera. Two immunoreactive fragments (36.4 and 34.8 kDa) were only generated by endoproteinase Glu-C treatment. These immunoreactive fragments or their parent molecules may be ideal candidates for incorporation in a cocktail vaccine against cutaneous leishmaniasis. Citations Citations to this article as recorded by Highly Effective Serodiagnosis for Chagas' Disease Pilar Hernández, Michael Heimann, Cristina Riera, Marco Solano, José Santalla, Alejandro O. Luquetti, Ewald Beck Clinical and Vaccine Immunology.2010; 17(10): 1598. CrossRef 7,788 View 92 Download Crossref Identification of novel Leishmania major antigens that elicit IgG2a response in resistant and susceptible mice Mohammad Reza Mohammadi, Majid Zeinali, Sussan K. Ardestani, Amina Kariminia Korean J Parasitol 2006;44(1):43-48. Published online March 20, 2006 DOI: https://doi.org/10.3347/kjp.2006.44.1.43 Abstract PDF Experimental murine models with high, intermediate and low levels of genetically based susceptibility to Leishmania major infection reproduce almost entire spectrum of clinical manifestations of the human disease. There are increasing non-comparative studies on immune responses against isolated antigens of L. major in different murine strains. The aim of the present study was to find out whether there is an antigen that can induce protective immune response in resistant and susceptible murine strains. To do that, crude antigenic extract of procyclic and metacyclic promastigotes of L. major was prepared and subjected to SDS-PAGE electrophoresis. Western-blotting was used to search for antigen(s) capable of raising high antibody level of IgG2a versus IgG1 in the sera of both infected resistant and susceptible strains. Two novel antigens from metacyclic promastigotes of L. major (140 and 152 kDa) were potentially able to induce specific dominant IgG2a responses in BALB/c and C57BL/6 mice. The 2 antigens also reacted with IgG antibody of cutaneous leishmaniasis patients. We confirm that 140 and 152 kDa proteins of L. major promastigotes are inducing IgG production in mice and humans. Citations Citations to this article as recorded by Protective Effects of rLPG3 Plus Freund’s Incomplete Adjuvant on Parasitism, Hepatic Function, and Immune Modulation in Experimental Visceral Leishmaniasis Daniel Silva Sena Bastos, Bianca Meirelles Miranda, Caroline Itagiba Rooke, Neverton José Silva Ferreira, Luiz Otávio Guimarães Ervilha, Renner Philipe Rodrigues Carvalho, Ana Cláudia Ferreira Souza, Mariana Machado Neves, Leandro Licursi de Oliveira, Edu ACS Infectious Diseases.2026; 12(2): 794. CrossRef Evaluation of Leishmania homolog of activated C kinase (LACK) of Leishmania donovani in comparison to glycoprotein 63 as a vaccine candidate against visceral leishmaniasis Nicky Didwania, Sudipta Bhowmick, Abdus Sabur, Anirban Bhattacharya, Nahid Ali, Monica R. Mugnier Microbiology Spectrum.2025;[Epub] CrossRef Diphencyprone reduces the CD8+ lymphocytes and IL-4 and enhences IgG2a/IgG1 ratio in pathogenicity of acute leishmania major infection in BALB/c mice Pourandokht Mousavian, Vahid Mashayekhi Goyonlo, Mohammad Javanbakht, Mahmoud Reza Jafari, Hamidreza Moosavian, Monovar Afzal Aghaei, Mohammadreza Malekzadeh Cytokine.2024; 184: 156792. CrossRef Immunological characterization of rLdTCP1γ for its prophylactic potential against visceral leishmaniasis in hamster model Apeksha Anand, Deep Chandra Balodi, Karthik Ramalingam, Shailendra Yadav, Neena Goyal Molecular Immunology.2022; 141: 33. CrossRef Adjuvanted vaccines driven protection against visceral infection in BALB/c mice by Leishmania donovani Deepak Kumar Goyal, Poonam Keshav, Sukhbir Kaur Microbial Pathogenesis.2021; 151: 104733. CrossRef Immune induction by adjuvanted Leishmania donovani vaccines against the visceral leishmaniasis in BALB/c mice Deepak Kumar Goyal, Poonam Keshav, Sukhbir Kaur Immunobiology.2021; 226(2): 152057. CrossRef Adjuvant effects of TLR agonist gardiquimod admixed with Leishmania vaccine in mice model of visceral leishmaniasis Deepak Kumar Goyal, Poonam Keshav, Sukhbir Kaur Infection, Genetics and Evolution.2021; 93: 104947. CrossRef The potential of live attenuated vaccines against Cutaneous Leishmaniasis A. Zabala-Peñafiel, D. Todd, H. Daneshvar, R. Burchmore Experimental Parasitology.2020; 210: 107849. CrossRef Immunization with a recombinant fusion protein protects mice against Helicobacter pylori infection Amir Ghasemi, Nazanin Mohammad, Josef Mautner, Mehrnaz Taghipour Karsabet, Jafar Amani, Abolfazl Ardjmand, Zarichehr Vakili Vaccine.2018; 36(34): 5124. CrossRef Evaluation of vaccinal effectiveness of preparations containing membrane antigens of Leishmania (L.) amazonensis in experimental cutaneous leishmaniasis model João G. Ribeiro, Amália S. Ferreira, Sharon R.A. Macedo, Norton R.D.L.P. Rossi, Mayara C.P. da Silva, Rosane N.M. Guerra, Neuza B. de Barros, Roberto Nicolete International Immunopharmacology.2017; 47: 227. CrossRef Evaluation of the immunoprophylactic potential of a killed vaccine candidate in combination with different adjuvants against murine visceral leishmaniasis Ankita Thakur, Harpreet Kaur, Sukhbir Kaur Parasitology International.2015; 64(1): 70. CrossRef Ox40L–Ox40 pathway plays distinct roles in regulating Th2 responses but does not determine outcome of cutaneous leishmaniasis caused by Leishmania mexicana and Leishmania major Rashmi Tuladhar, Steve Oghumu, Ran Dong, Allison Peterson, Arlene H. Sharpe, Abhay R. Satoskar Experimental Parasitology.2015; 148: 49. CrossRef Identification of immunodominant Leishmania major antigenic markers of the early C57BL/6 and BALB/c mice infection stages A. Sassi, O. Kaak, A. Ben Ammar Elgaied Parasite Immunology.2015; 37(10): 544. CrossRef Th1 Platform Immune Responses Against Leishmania major Induced by Thiol-Specific Antioxidant-Based DNA Vaccines Fatemeh Tabatabaie, Mehdi Mahdavi, Sobhan Faezi, Abdolhossein Dalimi, Zohreh Sharifi, Lame Akhlaghi, Fatemeh Ghaffarifar Jundishapur Journal of Microbiology.2014;[Epub] CrossRef Studies on the protective efficacy and immunogenicity of Hsp70 and Hsp83 based vaccine formulations in Leishmania donovani infected BALB/c mice Jaspreet Kaur, Tejinder Kaur, Sukhbir Kaur Acta Tropica.2011; 119(1): 50. CrossRef Protective effect of lectin from Synadenium carinatum on Leishmania amazonensis infection in BALB/c mice Sandra R. Afonso-Cardoso, Flávio H. Rodrigues, Márcio A.B. Gomes, Adriano G. Silva, Ademir Rocha, Aparecida H.B. GuimarÃes, Ignês Candeloro, Sílvio Favoreto, Marcelo S. Ferreira, Maria A. de Souza The Korean Journal of Parasitology.2007; 45(4): 255. CrossRef Purification and biochemical characterization of two novel antigens from Leishmania major promastigotes Majid Zeinali, Sussan K. Ardestani, Amina Kariminia The Korean Journal of Parasitology.2007; 45(4): 287. CrossRef 8,132 View 73 Download Crossref Production of nitric oxide by murine macrophages induced by lipophosphoglycan of Leishmania major Gholamreza Kavoosi, Sussan K. Ardestani, Amina Kariminia, Zahra Tavakoli Korean J Parasitol 2006;44(1):35-41. Published online March 20, 2006 DOI: https://doi.org/10.3347/kjp.2006.44.1.35 Abstract PDF Protozoan parasites of the genus Leishmania cause a number of important human diseases. One of the key determinants of parasite infectivity and survival is the surface glycoconjugate lipophosphoglycan (LPG). In addition, LPG is shown to be useful as a transmission blocking vaccine. Since culture supernatant of parasite promastigotes is a good source of LPG, we made attempts to characterize functions of the culture supernatant, and membrane LPG isolated from metacyclic promastigotes of Leishmania major. The purification scheme included anion-exchange chromatography, hydrophobic interaction chromatography and cold methanol precipitation. The purity of supernatant LPG (sLPG) and membrane LPG (mLPG) was determined by SDS-PAGE and thin layer chromatography. The effect of mLPG and sLPG on nitric oxide (NO) production by murine macrophages cell line (J774.1A) was studied. Both sLPG and mLPG induced NO production in a dose dependent manner but sLPG induced significantly higher amount of NO than mLPG. Our results show that sLPG is able to promote NO production by murine macrophages. Citations Citations to this article as recorded by Review on the Role of Host Immune Response in Protection and Immunopathogenesis during Cutaneous Leishmaniasis Infection Teshager Dubie, Yasin Mohammed, Patrice Xavier Petit Journal of Immunology Research.2020;[Epub] CrossRef Metformin partially reverses the inhibitory effect of co-culture with ER-/PR-/HER2+ breast cancer cells on biomarkers of monocyte antitumor activity Zoheir Dahmani, Lynda Addou-Klouche, Florence Gizard, Sara Dahou, Aida Messaoud, Nihel Chahinez Djebri, Mahmoud Idris Benaissti, Meriem Mostefaoui, Hadjer Terbeche, Wafa Nouari, Marwa Miliani, Gérard Lefranc, Anne Fernandez, Ned J. Lamb, Mourad Aribi, Sal PLOS ONE.2020; 15(10): e0240982. CrossRef Future Prospects in the Treatment of Parasitic Diseases: 2-Amino-1,3,4-Thiadiazoles in Leishmaniasis Georgeta Serban Molecules.2019; 24(8): 1557. CrossRef A new approach for development of vaccine against visceral leishmaniasis: Lipophosphoglycan and polyacrylic acid conjugates Adil M. Allahverdiyev, Rabia Cakir Koc, Melahat Bagirova, Serhat Elcicek, Serap Yesilkir Baydar, Olga Nehir Oztel, Emrah Sefik Abamor, Sezen Canim Ates, Murat Topuzogullari, Sevil Isoglu Dincer, Zeynep Akdeste Asian Pacific Journal of Tropical Medicine.2017; 10(9): 877. CrossRef Reactive Oxygen Species and Nitric Oxide in Cutaneous Leishmaniasis Maria Fátima Horta, Bárbara Pinheiro Mendes, Eric Henrique Roma, Fátima Soares Motta Noronha, Juan Pereira Macêdo, Luciana Souza Oliveira, Myrian Morato Duarte, Leda Quercia Vieira Journal of Parasitology Research.2012; 2012: 1. CrossRef DNA damage and nitric oxide production in mice following infection with L. chagasi Larissa Ragozo Cardoso de Oliveira, Gláucia Aparecida Gomes Cezário, Carlos Roberto Gonçalves de Lima, Vanessa Cristina Nicolete, Eliana Peresi, Maria Tereza de Síbio, Mariele Cristina Modolo Picka, Sueli Aparecida Calvi Mutation Research/Genetic Toxicology and Environmental Mutagenesis.2011; 723(2): 177. CrossRef Antileishmanial Activity of 1,3,4-Thiadiazolium-2-Aminide in Mice Infected withLeishmania amazonensis Raquel F. Rodrigues, Karen S. Charret, Edson F. da Silva, Áurea Echevarria, Verônica F. Amaral, Leonor L. Leon, Marilene M. Canto-Cavalheiro Antimicrobial Agents and Chemotherapy.2009; 53(2): 839. CrossRef Pharmacological evaluation of anti-leishmanial activity by in vivo nitric oxide modulation in Balb/c mice infected with Leishmania major MRHO/IR/75/ER: An Iranian strain of cutaneous leishmaniasis Hossein Nahrevanian, Mahin Farahmand, Zohreh Aghighi, Mehdi Assmar, Aref Amirkhani Experimental Parasitology.2007; 116(3): 233. CrossRef 8,714 View 86 Download Crossref