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Tongjit Thanchomnang	5 Articles
Molecular Variation in the Paragonimus heterotremus Complex in Thailand and Myanmar Oranuch Sanpool, Pewpan M. Intapan, Tongjit Thanchomnang, Penchom Janwan, Yukifumi Nawa, David Blair, Wanchai Maleewong Korean J Parasitol 2013;51(6):677-681. Published online December 31, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.6.677 Abstract PDF Paragonimiasis is an important food-borne parasitic zoonosis caused by infection with lung flukes of the genus Paragonimus. Of the 7 members of the genus known in Thailand until recently, only P. heterotremus has been confirmed as causing human disease. An 8th species, P. pseudoheterotremus, has recently been proposed from Thailand, and has been found in humans. Molecular data place this species as a sister species to P. heterotremus, and it is likely that P. pseudoheterotremus is not specifically distinct from P. heterotremus. In this study, we collected metacercariae of both nominal species (identification based on metacercarial morphology) from freshwater crabs from Phetchabun Province in northern Thailand, Saraburi Province in central Thailand, and Surat Thani Province in southern Thailand. In addition, we purchased freshwater crabs imported from Myanmar at Myawaddy Province, western Thailand, close to the Myanmar-Thailand border. The DNAs extracted from excysted metacercariae were PCR-amplified and sequenced for ITS2 and cox1 genes. The ITS2 sequences were nearly identical among all samples (99-100%). Phylogenies inferred from all available partial cox1 sequences contained several clusters. Sequences from Indian P. heterotremus formed a sister group to sequences from P. pseudoheterotremus-type metacercariae. Sequences of P. heterotremus from Thailand, Vietnam, and China formed a separate distinct clade. One metacercaria from Phitsanulok Province was distinct from all others. There is clearly considerable genetic variation in the P. heterotremus complex in Thailand and the form referred to as P. pseudoheterotremus is widely distributed in Thailand and the Thai-Myanmar border region. Citations Citations to this article as recorded by The global distribution of Paragonimus species Yuan-Biao Lu, Kai Hu, Mei-Lin Mao, Lin-Bo Shi, Chun-Chao Zhu, Jie-Xin Zou Infectious Diseases of Poverty.2026;[Epub] CrossRef Considerable genetic diversity within Paragonimus heterotremus in Luang Prabang, northern Lao People's Democratic Republic Alongkorn Nonthapa, Rutchanee Rodpai, Tongjit Thanchomnang, Patcharaporn Boonroumkaew, Lakkhana Sadaow, David Blair, Pewpan M. Intapan, Wanchai Maleewong, Virasack Banouvong, Sakhone Laymanivong, Oranuch Sanpool Infection, Genetics and Evolution.2025; 128: 105718. CrossRef Nuclear ribosomal transcription units in Asian Paragonimus species (Paragonimidae: Platyhelminthes): genetic characteristics, polymorphism, and implications for intersuperfamilial phylogeny Khue Thi Nguyen, Huong Thi Thanh Doan, Linh Thi Khanh Pham, Do Thi Roan, Takeshi Agatsuma, Pham Ngoc Doanh, Thanh Hoa Le Parasitology Research.2024;[Epub] CrossRef Morphological and Molecular Characterization of Paragonimus skrjabini Complex from Yunnan, China: A Brief Report Qiu-Hong Shu, Shu-De Li, Ming Tian, Yong Meng, Shu-Mei-Qi He, Min Zhu, Miao-Miao Wang, Wen-Lin Wang Acta Parasitologica.2022; 67(1): 316. CrossRef The potential use of mitochondrial ribosomal genes (12S and 16S) in DNA barcoding and phylogenetic analysis of trematodes Abigail Hui En Chan, Naowarat Saralamba, Sompob Saralamba, Jiraporn Ruangsittichai, Urusa Thaenkham BMC Genomics.2022;[Epub] CrossRef General overview of the current status of human foodborne trematodiasis Jong-Yil Chai, Bong-Kwang Jung Parasitology.2022; 149(10): 1262. CrossRef Genetic differentiation of Southeast Asian Paragonimus Braun, 1899 (Digenea: Paragonimidae) and genetic variation in the Paragonimus heterotremus complex examined by nuclear DNA sequences Chairat Tantrawatpan, Sumonta Tapdara, Takeshi Agatsuma, Oranuch Sanpool, Pewpan M. Intapan, Wanchai Maleewong, Weerachai Saijuntha Infection, Genetics and Evolution.2021; 90: 104761. CrossRef An outbreak of Paragonimus westermani infection among Cambodian technical intern trainees in Japan, exhibiting various extrapulmonary lesions Yoshifumi Nakagawa, Yuki Ikematsu, Takayuki Nakanishi, Yuji Ogawa, Ryuichi Taen, Yasunori Nakashima, Hironao Okabe, Ayako Yoshida, Haruhiko Maruyama Parasitology International.2021; 81: 102279. CrossRef Development of point-of-care testing tool using immunochromatography for rapid diagnosis of human paragonimiasis Lakkhana Sadaow, Oranuch Sanpool, Hiroshi Yamasaki, Wanchai Maleewong, Pewpan M. Intapan Acta Tropica.2020; 203: 105325. CrossRef Paragonimus and paragonimiasis in Asia: An update Ayako Yoshida, Pham Ngoc Doanh, Haruhiko Maruyama Acta Tropica.2019; 199: 105074. CrossRef Morphological and molecular characterization of Paragonimus caliensis Little, 1968 (Trematoda: Paragonimidae) from Medellin and Pichinde, Colombia Carolina Lenis, Alicia Galiano, Imelda Vélez, Iván Darío Vélez, Carlos Muskus, Antonio Marcilla Acta Tropica.2018; 183: 95. CrossRef Gene diversity and genetic variation in lung flukes (genusParagonimus) David Blair, Yukifumi Nawa, Makedonka Mitreva, Pham Ngoc Doanh Transactions of The Royal Society of Tropical Medicine and Hygiene.2016; 110(1): 6. CrossRef A review on the molecular characterization of digenean parasites using molecular markers with special reference to ITS region K. Choudhary, A. Kumar Verma, S. Swaroop, N. Agrawal Helminthologia.2015; 52(3): 167. CrossRef 11,892 View 117 Download Crossref Molecular Detection of Ancylostoma duodenale, Ancylostoma ceylanicum, and Necator americanus in Humans in Northeastern and Southern Thailand Issarapong Phosuk, Pewpan M. Intapan, Tongjit Thanchomnang, Oranuch Sanpool, Penchom Janwan, Porntip Laummaunwai, Witthaya Aamnart, Nimit Morakote, Wanchai Maleewong Korean J Parasitol 2013;51(6):747-749. Published online December 31, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.6.747 Abstract PDF The 2 principal species of hookworms infecting humans are Necator americanus and Ancylostoma duodenale. Case studies on zoonotic hookworm infections with Ancylostoma ceylanicum and/or Ancylostoma caninum are known mainly from Asian countries. Of these 2 zoonotic species, only A. ceylanicum can develop to adulthood in humans. In the present study, we report a molecular-based survey of human hookworm infections present in southern and northeastern Thailand. Thirty larval hookworm samples were obtained from fecal agar plate cultures of 10 patients in northeastren Thailand and 20 in southern Thailand. Partial ITS1, 5.8S, and ITS2 regions of the ribosomal DNA genes were amplified using PCR. The amplicons were sequenced, aligned, and compared with other hookworm sequences in GenBank database. The results showed that, in Thailand, N. americanus is more prevalent than Ancylostoma spp. and is found in both study areas. Sporadic cases of A. ceylanicum and A. duodenale infection were seen in northeastern Thailand. Citations Citations to this article as recorded by Poor Response to Albendazole in Hookworm‐Infected Abnormal Haemoglobin Carriers Attarat Pattanawongsa, Manit Nuinoon, Prasit Na‐ek, Patthanasak Khammaneechan, Blego Sedionoto, Witthaya Anamnart Tropical Medicine & International Health.2026;[Epub] CrossRef Prevalence, risk factors, and zoonotic potential of gastrointestinal helminths in cats: A participatory epidemiological study S. Avachare, M. Singh, M. Shakya, A.K. Sharma, N. Bhangale, A. Mourya, G.P. Jatav, N.S. Choudhary Journal of Helminthology.2026;[Epub] CrossRef Efficacy of Simparica Trio® against induced infections of Ancylostoma braziliense and Ancylostoma ceylanicum in dogs Raj Packianathan, Andrew Hodge, Natalie Bruellke, Michael Pearce, Frans Selepe, Piyanan Taweethavonsawat, Thomas Geurden Parasites & Vectors.2025;[Epub] CrossRef The occurrence of cross-host species soil-transmitted helminth infections in humans and domestic/livestock animals: A systematic review Uniqueky Gratis Mawrie, Riviarynthia Kharkongor, María Martínez Valladares, Stella Kepha, Sitara S. R. Ajjampur, Rajiv Sarkar, Rachel Pullan, Muhammad Asaduzzaman PLOS Global Public Health.2025; 5(8): e0004614. CrossRef Development of loop mediated isothermal amplification assay for rapid detection and its comparison with PCR techniques for Ancylostoma duodenale Mamta Thakur, Abhishek Mewara, P. V. M. Lakshmi, Sucheta Guleria, Sumeeta Khurana Scientific Reports.2025;[Epub] CrossRef PERFIL PARASITOLÓGICO DE HORTALIÇAS CULTIVADAS EM HORTAS TRADICIONAIS Maria Beatriz Neves Nascimento, Monalissa Dias de Souza, Alexandro de Andrade de Lima, Gislaine Cristina de Souza Melanda, Renato Juciano Ferreira Revista Saúde - UNG-Ser.2025;[Epub] CrossRef Ancylostoma ceylanicum and other zoonotic canine hookworms: neglected public and animal health risks in the Asia–Pacific region Jan Clyden B. Tenorio, Ian Kim B. Tabios, Tawin Inpankaew, Adrian P. Ybañez, Saruda Tiwananthagorn, Sirikachorn Tangkawattana, Sutas Suttiprapa Animal Diseases.2024;[Epub] CrossRef Molecular identification and genetic diversity of zoonotic hookworm infections in domestic dogs from northeastern, Thailand Rutchanee Rodpai, Oranuch Sanpool, Lakkhana Sadaow, Patcharaporn Boonroumkaew, Pewpan M. Intapan, Wanchai Maleewong, Manachai Yingklang, Penchom Janwan, Kotchaphon Vaisusuk, Wasupon Chatan, Supawadee Piratae, Tongjit Thanchomnang Parasitology Research.2024;[Epub] CrossRef The enhancive effect of the 2014–2016 El Niño-induced drought on the control of soil-transmitted helminthiases without anthelmintics: A longitudinal study Attarat Pattanawongsa, Pattanasak Kammaneechan, Prasit Na-ek, Blego Sedionoto, Witthaya Anamnart, Qu Cheng PLOS Neglected Tropical Diseases.2024; 18(7): e0012331. CrossRef Assessment of an Immuno-Diagnostic Method for Hookworm-Related Cutaneous Larva Migrans Using Crude Extracts of Ancylostoma caninum Sitthithana Adam, Paron Dekumyoy, Duangporn Nacapunchai, Thawatchai Ketboonlue, Prakaykaew Charunwatthana, Jittima Dhitavat, Khuanchai Koompapong, Putza Chonsawat, Dorn Watthanakulpanich Tropical Medicine and Infectious Disease.2023; 8(4): 209. CrossRef Global distribution of human hookworm species and differences in their morbidity effects: a systematic review Archie C A Clements, Kefyalew Addis Alene The Lancet Microbe.2022; 3(1): e72. CrossRef Comparison of percutaneous vs oral infection of hamsters with the hookworm Ancylostoma ceylanicum: Parasite development, pathology and primary immune response Richard D. Bungiro, Lisa M. Harrison, Blaise Dondji, Michael Cappello, Maria Angeles Gómez-Morales PLOS Neglected Tropical Diseases.2022; 16(1): e0010098. CrossRef Prevalence of intestinal parasitic infections and genetic differentiation of Strongyloides stercoralis among migrant workers from Myanmar, Lao PDR and Cambodia in northeastern Thailand Wararat Sangwalee, Jun Norkaew, Sengchoy Inthachak, Penchom Janwan, Rutchanee Rodpai, Oranuch Sanpool, Lakkhana Sadaow, Patcharaporn Boonroumkaew, Pewpan M. Intapan, Wanchai Maleewong, Tongjit Thanchomnang, Raffi V. Aroian PLOS ONE.2022; 17(12): e0279754. CrossRef Copro-molecular identification of intestinal nematode infections in a rural community in East Malaysia Nur-Amirah Hassan, Autchariya Thodsapol, Yvonne Ai Lian Lim, Wan Yusoff Wan Sulaiman, Siti Farah Norasyikeen Sidi Omar, Kamontam Umsakul, Romano Ngui Parasitology International.2021; 80: 102237. CrossRef Soil-transmitted helminths, intestinal protozoa and Clonorchis sinensis infections in southeast China Yan Feng, Kegen Yu, Hualiang Chen, Xuan Zhang, Qiaoyi Lu, Xiaoxiao Wang, Xueying Zhang, Linong Yao, Wei Ruan BMC Infectious Diseases.2021;[Epub] CrossRef Zoonotic hookworms of dogs and cats – lessons from the past to inform current knowledge and future directions of research Rebecca J. Traub, Patsy A. Zendejas-Heredia, Luca Massetti, Vito Colella International Journal for Parasitology.2021; 51(13-14): 1233. CrossRef Molecular identification of hookworm isolates from stray dogs, humans and selected wildlife from South Africa P. I. Ngcamphalala, J. Lamb, S. Mukaratirwa Journal of Helminthology.2020;[Epub] CrossRef Semi-domesticated dogs as a potential reservoir for zoonotic hookworms in Bangkok, Thailand Jutamas Wongwigkan, Tawin Inpankaew Veterinary World.2020; 13(5): 909. CrossRef The increased sensitivity of qPCR in comparison to Kato-Katz is required for the accurate assessment of the prevalence of soil-transmitted helminth infection in settings that have received multiple rounds of mass drug administration Julia C. Dunn, Marina Papaiakovou, Kay Thwe Han, Darren Chooneea, Alison A. Bettis, Nay Yee Wyine, Aye Moe Moe Lwin, Nay Soe Maung, Raju Misra, D. T. J. Littlewood, Roy M. Anderson Parasites & Vectors.2020;[Epub] CrossRef Zoonotic Ancylostomiasis: An Update of a Continually Neglected Zoonosis Katharina Stracke, Aaron R. Jex, Rebecca J. Traub The American Journal of Tropical Medicine and Hygiene.2020; 103(1): 64. CrossRef Of dogs and hookworms: man’s best friend and his parasites as a model for translational biomedical research Catherine Shepherd, Phurpa Wangchuk, Alex Loukas Parasites & Vectors.2018;[Epub] CrossRef Prevalence of Hookworm infection and Strongyloidiasis in Cats and Potential Risk Factor of Human Diseases Blego Sedionoto, Witthaya Anamnart, Hadiyanto, Sudarno, Maryono E3S Web of Conferences.2018; 31: 06002. CrossRef Molecular characterization of hookworm spp. isolated from food handlers, Khartoum, Sudan: A cross-sectional study Tarig A. Gamar, Hassan H. Musa, Hisham N. Altayb, Mohamed H. Mohamed, Adam D. Abakar F1000Research.2018; 7: 662. CrossRef Ancylostoma ceylanicum Hookworm in Myanmar Refugees, Thailand, 2012–2015 Elise M. O’Connell, Tarissa Mitchell, Marina Papaiakovou, Nils Pilotte, Deborah Lee, Michelle Weinberg, Potsawin Sakulrak, Dilok Tongsukh, Georgiette Oduro-Boateng, Sarah Harrison, Steven A. Williams, William M. Stauffer, Thomas B. Nutman Emerging Infectious Diseases.2018;[Epub] CrossRef Development and Evaluation of a Multiplex Quantitative Real-Time Polymerase Chain Reaction for Hookworm Species in Human Stool Sze Fui Hii, Dammika Senevirathna, Stacey Llewellyn, Tawin Inpankaew, Peter Odermatt, Virak Khieu, Sinoun Muth, James McCarthy, Rebecca J. Traub The American Journal of Tropical Medicine and Hygiene.2018; 99(5): 1186. CrossRef Hookworm infections among migrant workers in Malaysia: Molecular identification of Necator americanus and Ancylostoma duodenale Norhidayu Sahimin, Yvonne Ai Lian Lim, Benacer Douadi, Mohd Khairul Nizam Mohd Khalid, John-James Wilson, Jerzy M. Behnke, Siti Nursheena Mohd Zain Acta Tropica.2017; 173: 109. CrossRef Prevalence of intestinal parasitic infection and associated risk factors among village health volunteers in rural communities of southern Thailand Chuchard Punsawad, Nonthapan Phasuk, Suchirat Bunratsami, Kanjana Thongtup, Niramon Siripakonuaong, Somchok Nongnaul BMC Public Health.2017;[Epub] CrossRef Soil-Transmitted Helminths in Tropical Australia and Asia Catherine Gordon, Johanna Kurscheid, Malcolm Jones, Darren Gray, Donald McManus Tropical Medicine and Infectious Disease.2017; 2(4): 56. CrossRef Restoration of hookworm egg development after prolonged storage in stool suspension Prasit Na-Ek, Oranuch Sanpool, Jurairat Jongthawin, Witthaya Anamnart, Pewpan M. Intapan, Pennapa Chamavit, Wanchai Maleewong Parasitology Research.2016; 115(7): 2817. CrossRef Prevalence of gastrointestinal helminth parasites of zoonotic significance in dogs and cats in lower Northern Thailand Wilawan PUMIDONMING, Doaa SALMAN, Dulyatad GRONSANG, Abdelbaset E. ABDELBASET, Khamphon SANGKAEO, Shin-ichiro KAWAZU, Makoto IGARASHI Journal of Veterinary Medical Science.2016; 78(12): 1779. CrossRef 12,343 View 305 Download Crossref Molecular Differentiation of Schistosoma japonicum and Schistosoma mekongi by Real-Time PCR with High Resolution Melting Analysis Amornmas Kongklieng, Worasak Kaewkong, Pewpan M. Intapan, Oranuch Sanpool, Penchom Janwan, Tongjit Thanchomnang, Viraphong Lulitanond, Pusadee Sri-Aroon, Yanin Limpanont, Wanchai Maleewong Korean J Parasitol 2013;51(6):651-656. Published online December 31, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.6.651 Abstract PDF Human schistosomiasis caused by Schistosoma japonicum and Schistosoma mekongi is a chronic and debilitating helminthic disease still prevalent in several countries of Asia. Due to morphological similarities of cercariae and eggs of these 2 species, microscopic differentiation is difficult. High resolution melting (HRM) real-time PCR is developed as an alternative tool for the detection and differentiation of these 2 species. A primer pair was designed for targeting the 18S ribosomal RNA gene to generate PCR products of 156 base pairs for both species. The melting points of S. japonicum and S. mekongi PCR products were 84.5±0.07℃ and 85.7±0.07℃, respectively. The method permits amplification from a single cercaria or an egg. The HRM real-time PCR is a rapid and simple tool for differentiation of S. japonicum and S. mekongi in the intermediate and final hosts. Citations Citations to this article as recorded by Current advances in serological and molecular diagnosis of Schistosoma mekongi infection Adrian Miki C. Macalanda, Atcharaphan Wanlop, Kevin Austin L. Ona, Eloiza May S. Galon, Virak Khieu, Somphou Sayasone, Aya Yajima, Jose Ma. M. Angeles, Shin-ichiro Kawazu Tropical Medicine and Health.2024;[Epub] CrossRef Molecular Techniques as Alternatives of Diagnostic Tools in China as Schistosomiasis Moving towards Elimination Chao Lv, Wangping Deng, Liping Wang, Zhiqiang Qin, Xiaonong Zhou, Jing Xu Pathogens.2022; 11(3): 287. CrossRef Development of a novel real-time polymerase chain reaction assay for the sensitive detection of Schistosoma japonicum in human stool Sara Halili, Jessica R. Grant, Nils Pilotte, Catherine A. Gordon, Steven A. Williams, Cinzia Cantacessi PLOS Neglected Tropical Diseases.2021; 15(10): e0009877. CrossRef Establishment and application of a novel fluorescence-based analytical method for the rapid detection of viable bacteria in different samples Qiuyue Yin, Maiqian Nie, Zhenjun Diwu, Yuting Zhang, Lei Wang, Dandan Yin, Liancheng Li Analytical Methods.2020; 12(31): 3933. CrossRef Molecular characterization and functional analysis of the Schistosoma mekongi Ca2+-dependent cysteine protease (calpain) Salisa Chaimon, Yanin Limpanont, Onrapak Reamtong, Sumate Ampawong, Orawan Phuphisut, Phiraphol Chusongsang, Jiraporn Ruangsittichai, Usa Boonyuen, Dorn Watthanakulpanich, Anthony J. O’Donoghue, Conor R. Caffrey, Poom Adisakwattana Parasites & Vectors.2019;[Epub] CrossRef Rapid detection and identification of four major Schistosoma species by high-resolution melt (HRM) analysis Juan Li, Guang-Hui Zhao, RuiQing Lin, David Blair, Hiromu Sugiyama, Xing-Quan Zhu Parasitology Research.2015; 114(11): 4225. CrossRef Detection of Schistosoma mansoni and Schistosoma haematobium by Real-Time PCR with High Resolution Melting Analysis Hany Sady, Hesham Al-Mekhlafi, Romano Ngui, Wahib Atroosh, Ahmed Al-Delaimy, Nabil Nasr, Salwa Dawaki, Awatif Abdulsalam, Init Ithoi, Yvonne Lim, Kek Chua, Johari Surin International Journal of Molecular Sciences.2015; 16(7): 16085. CrossRef Bacterial detection: From microscope to smartphone Subash C.B. Gopinath, Thean-Hock Tang, Yeng Chen, Marimuthu Citartan, Thangavel Lakshmipriya Biosensors and Bioelectronics.2014; 60: 332. CrossRef 10,628 View 104 Download Crossref Rapid Detection and Identification of Wuchereria bancrofti, Brugia malayi, B. pahangi, and Dirofilaria immitis in Mosquito Vectors and Blood Samples by High Resolution Melting Real-Time P Tongjit Thanchomnang, Pewpan M. Intapan, Chairat Tantrawatpan, Viraphong Lulitanond, Sudchit Chungpivat, Piyanan Taweethavonsawat, Worasak Kaewkong, Oranuch Sanpool, Penchom Janwan, Wej Choochote, Wanchai Maleewong Korean J Parasitol 2013;51(6):645-650. Published online December 31, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.6.645 Abstract PDF A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at 81.5±0.2℃, 79.0±0.3℃, 76.8±0.1℃, and 79.9±0.1℃, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors. Citations Citations to this article as recorded by Beyond Tradition: Exploring Cutting-Edge Approaches for Accurate Diagnosis of Human Filariasis Damian Pietrzak, Julia Weronika Łuczak, Marcin Wiśniewski Pathogens.2024; 13(6): 447. CrossRef Detecting Dirofilaria immitis: Current Practices and Novel Diagnostic Methods Damian Pietrzak, Julia Weronika Łuczak, Marcin Wiśniewski Pathogens.2024; 13(11): 950. CrossRef IDENTIFICATION OF MICROFILARIAE USING CONVENTIONAL POLYMERASE CHAIN REACTION AND QPCR-HRM Bagus Muhammad Ihsan, Widyana Lakshmi Puspita, Linda Triana, Wahdaniah, Khayan, Cecep Dani Sucipto Journal of Vocational Health Studies.2024; 8(1): 42. CrossRef Mosquitoes, Lymphatic Filariasis, and Public Health: A Systematic Review of Anopheles and Aedes Surveillance Strategies Arumugam Bhuvaneswari, Ananganallur Nagarajan Shriram, Kishan Hari K. Raju, Ashwani Kumar Pathogens.2023; 12(12): 1406. CrossRef Microfilaria Positification Test Using Real-Time PCR Technique with HRM (High-Resolution Melting) Bagus Muhammad Ihsan, Afiat Berbudi, Ridad Agoes, Muhammad Ifham Hanif Acta Parasitologica.2022; 67(1): 496. CrossRef Collection and DNA Detection of Dirofilaria immitis (Rhabditida Onchocercidae), Using a Novel Primer Set, in Wild-Caught Mosquitoes From Gainesville, FL Chris Holderman, Nicole O Abruzzo, Noor A Abdelsamad, Phillip E Kaufman, Peter M DiGennaro, Stephen Rich Journal of Medical Entomology.2021; 58(3): 1429. CrossRef The use of molecular xenomonitoring for surveillance of mosquito-borne diseases Mary M. Cameron, Anita Ramesh Philosophical Transactions of the Royal Society B: Biological Sciences.2021; 376(1818): 20190816. CrossRef Canine and feline vector-borne diseases of zoonotic concern in Southeast Asia Viet-Linh Nguyen, Filipe Dantas-Torres, Domenico Otranto Current Research in Parasitology & Vector-Borne Diseases.2021; 1: 100001. CrossRef The Incidence of Dirofilaria immitis in Shelter Dogs and Mosquitoes in Austria Karin Sonnberger, Hans-Peter Fuehrer, Bernhard Werner Sonnberger, Michael Leschnik Pathogens.2021; 10(5): 550. CrossRef Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases Madeleine J. Rogers, Donald P. McManus, Stephen Muhi, Catherine A. Gordon Clinical Microbiology Reviews.2021;[Epub] CrossRef PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION Bagus Muhammad Ihsan, Cecep Dani Sucipto, Khayan Khayan Journal of Vocational Health Studies.2021; 5(1): 26. CrossRef Molecular detection of Trichostrongylus species through PCR followed by high resolution melt analysis of ITS-2 rDNA sequences Mohsen Arbabi, Hossein Hooshyar, Majid Lotfinia, Mohamad Ali Bakhshi Molecular and Biochemical Parasitology.2020; 236: 111260. CrossRef Prevalence and risk factors associated with Dirofilaria immitis infection in dogs and cats in Songkhla and Satun provinces, Thailand Ketsarin Kamyingkird, Witchuta Junsiri, Witsanuwat Chimnoi, Chanya Kengradomkij, Sinsamut Saengow, Keplee Sangchuto, Wicha Kajeerum, Decha Pangjai, Burin Nimsuphan, Tawin Inpankeaw, Sathaporn Jittapalapong Agriculture and Natural Resources.2017; 51(4): 299. CrossRef Is molecular xenomonitoring of mosquitoes for Dirofilaria repens suitable for dirofilariosis surveillance in endemic regions? Aleksander Masny, Rusłan Sałamatin, Wioletta Rozej-Bielicka, Elzbieta Golab Parasitology Research.2016; 115(2): 511. CrossRef Pyrosequencing Using SL and 5S rRNA as Molecular Markers for Identifying Zoonotic Filarial Nematodes in Blood Samples and Mosquitoes Oranuch Sanpool, Chairat Tantrawatpan, Tongjit Thanchomnang, Penchom Janwan, Pewpan M. Intapan, Rutchanee Rodpai, Viraphong Lulitanond, Piyanan Taweethavonsawat, Wanchai Maleewong Vector-Borne and Zoonotic Diseases.2016; 16(5): 326. CrossRef Detection and quantification of Wuchereria bancrofti and Brugia malayi DNA in blood samples and mosquitoes using duplex droplet digital polymerase chain reaction Jurairat Jongthawin, Pewpan M. Intapan, Viraphong Lulitanond, Oranuch Sanpool, Tongjit Thanchomnang, Lakkhana Sadaow, Wanchai Maleewong Parasitology Research.2016; 115(8): 2967. CrossRef Trypanosome infection rates in tsetse flies in the “silent” sleeping sickness focus of Bafia in the Centre Region in Cameroon Gustave Simo, Pierre Fongho, Oumarou Farikou, Prosper Innocent Ndjeuto Ndjeuto-Tchouli, Judith Tchouomene-Labou, Flobert Njiokou, Tazoacha Asonganyi Parasites & Vectors.2015;[Epub] CrossRef 12,262 View 139 Download Crossref Molecular Differentiation of Opisthorchis viverrini and Clonorchis sinensis Eggs by Multiplex Real-Time PCR with High Resolution Melting Analysis Worasak Kaewkong, Pewpan M. Intapan, Oranuch Sanpool, Penchom Janwan, Tongjit Thanchomnang, Porntip Laummaunwai, Viraphong Lulitanond, Pham Ngoc Doanh, Wanchai Maleewong Korean J Parasitol 2013;51(6):689-694. Published online December 31, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.6.689 Abstract PDF Opisthorchis viverrini and Clonorchis sinensis are parasites known to be carcinogenic and causative agents of cholangiocarcinoma in Asia. The standard method for diagnosis for those parasite infections is stool examination to detect parasite eggs. However, the method has low sensitivity, and eggs of O. viverrini and C. sinensis are difficult to distinguish from each other and from those of some other trematodes. Here, we report a multiplex real-time PCR coupled with high resolution melting (HRM) analysis for the differentiation of O. viverrini and C. sinensis eggs in fecal samples. Using 2 pairs of species-specific primers, DNA sequences from a portion of the mitochondrial NADH dehydrogenase subunit 2 (nad 2) gene, were amplified to generate 209 and 165 bp products for O. viverrini and C. sinensis, respectively. The distinct characteristics of HRM patterns were analyzed, and the melting temperatures peaked at 82.4±0.09℃ and 85.9±0.08℃ for O. viverrini and C. sinensis, respectively. This technique was able to detect as few as 1 egg of O. viverrini and 2 eggs of C. sinensis in a 150 mg fecal sample, which is equivalent to 7 and 14 eggs per gram of feces, respectively. The method is species-specific, rapid, simple, and does not require fluorescent probes or post-PCR processing for discrimination of eggs of the 2 species. It offers a new tool for differentiation and detection of Asian liver fluke infections in stool specimens. Citations Citations to this article as recorded by Multiparasitism in Spain in a Korean Visiting Friends and Relatives: Case Report and Review of Imported Cases of Clonorchis sinensis in Europe Paola Cociancic, Jacklyn Comas, J. 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