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Viraphong Lulitanond	4 Articles
Molecular Differentiation of Schistosoma japonicum and Schistosoma mekongi by Real-Time PCR with High Resolution Melting Analysis Amornmas Kongklieng, Worasak Kaewkong, Pewpan M. Intapan, Oranuch Sanpool, Penchom Janwan, Tongjit Thanchomnang, Viraphong Lulitanond, Pusadee Sri-Aroon, Yanin Limpanont, Wanchai Maleewong Korean J Parasitol 2013;51(6):651-656. Published online December 31, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.6.651 Abstract PDF Human schistosomiasis caused by Schistosoma japonicum and Schistosoma mekongi is a chronic and debilitating helminthic disease still prevalent in several countries of Asia. Due to morphological similarities of cercariae and eggs of these 2 species, microscopic differentiation is difficult. High resolution melting (HRM) real-time PCR is developed as an alternative tool for the detection and differentiation of these 2 species. A primer pair was designed for targeting the 18S ribosomal RNA gene to generate PCR products of 156 base pairs for both species. The melting points of S. japonicum and S. mekongi PCR products were 84.5±0.07℃ and 85.7±0.07℃, respectively. The method permits amplification from a single cercaria or an egg. The HRM real-time PCR is a rapid and simple tool for differentiation of S. japonicum and S. mekongi in the intermediate and final hosts. Citations Citations to this article as recorded by Current advances in serological and molecular diagnosis of Schistosoma mekongi infection Adrian Miki C. Macalanda, Atcharaphan Wanlop, Kevin Austin L. Ona, Eloiza May S. Galon, Virak Khieu, Somphou Sayasone, Aya Yajima, Jose Ma. M. Angeles, Shin-ichiro Kawazu Tropical Medicine and Health.2024;[Epub] CrossRef Molecular Techniques as Alternatives of Diagnostic Tools in China as Schistosomiasis Moving towards Elimination Chao Lv, Wangping Deng, Liping Wang, Zhiqiang Qin, Xiaonong Zhou, Jing Xu Pathogens.2022; 11(3): 287. CrossRef Development of a novel real-time polymerase chain reaction assay for the sensitive detection of Schistosoma japonicum in human stool Sara Halili, Jessica R. Grant, Nils Pilotte, Catherine A. Gordon, Steven A. Williams, Cinzia Cantacessi PLOS Neglected Tropical Diseases.2021; 15(10): e0009877. CrossRef Establishment and application of a novel fluorescence-based analytical method for the rapid detection of viable bacteria in different samples Qiuyue Yin, Maiqian Nie, Zhenjun Diwu, Yuting Zhang, Lei Wang, Dandan Yin, Liancheng Li Analytical Methods.2020; 12(31): 3933. CrossRef Molecular characterization and functional analysis of the Schistosoma mekongi Ca2+-dependent cysteine protease (calpain) Salisa Chaimon, Yanin Limpanont, Onrapak Reamtong, Sumate Ampawong, Orawan Phuphisut, Phiraphol Chusongsang, Jiraporn Ruangsittichai, Usa Boonyuen, Dorn Watthanakulpanich, Anthony J. O’Donoghue, Conor R. Caffrey, Poom Adisakwattana Parasites & Vectors.2019;[Epub] CrossRef Rapid detection and identification of four major Schistosoma species by high-resolution melt (HRM) analysis Juan Li, Guang-Hui Zhao, RuiQing Lin, David Blair, Hiromu Sugiyama, Xing-Quan Zhu Parasitology Research.2015; 114(11): 4225. CrossRef Detection of Schistosoma mansoni and Schistosoma haematobium by Real-Time PCR with High Resolution Melting Analysis Hany Sady, Hesham Al-Mekhlafi, Romano Ngui, Wahib Atroosh, Ahmed Al-Delaimy, Nabil Nasr, Salwa Dawaki, Awatif Abdulsalam, Init Ithoi, Yvonne Lim, Kek Chua, Johari Surin International Journal of Molecular Sciences.2015; 16(7): 16085. CrossRef Bacterial detection: From microscope to smartphone Subash C.B. Gopinath, Thean-Hock Tang, Yeng Chen, Marimuthu Citartan, Thangavel Lakshmipriya Biosensors and Bioelectronics.2014; 60: 332. CrossRef 10,628 View 104 Download Crossref Rapid Detection and Identification of Wuchereria bancrofti, Brugia malayi, B. pahangi, and Dirofilaria immitis in Mosquito Vectors and Blood Samples by High Resolution Melting Real-Time P Tongjit Thanchomnang, Pewpan M. Intapan, Chairat Tantrawatpan, Viraphong Lulitanond, Sudchit Chungpivat, Piyanan Taweethavonsawat, Worasak Kaewkong, Oranuch Sanpool, Penchom Janwan, Wej Choochote, Wanchai Maleewong Korean J Parasitol 2013;51(6):645-650. Published online December 31, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.6.645 Abstract PDF A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at 81.5±0.2℃, 79.0±0.3℃, 76.8±0.1℃, and 79.9±0.1℃, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors. Citations Citations to this article as recorded by Beyond Tradition: Exploring Cutting-Edge Approaches for Accurate Diagnosis of Human Filariasis Damian Pietrzak, Julia Weronika Łuczak, Marcin Wiśniewski Pathogens.2024; 13(6): 447. CrossRef Detecting Dirofilaria immitis: Current Practices and Novel Diagnostic Methods Damian Pietrzak, Julia Weronika Łuczak, Marcin Wiśniewski Pathogens.2024; 13(11): 950. CrossRef IDENTIFICATION OF MICROFILARIAE USING CONVENTIONAL POLYMERASE CHAIN REACTION AND QPCR-HRM Bagus Muhammad Ihsan, Widyana Lakshmi Puspita, Linda Triana, Wahdaniah, Khayan, Cecep Dani Sucipto Journal of Vocational Health Studies.2024; 8(1): 42. CrossRef Mosquitoes, Lymphatic Filariasis, and Public Health: A Systematic Review of Anopheles and Aedes Surveillance Strategies Arumugam Bhuvaneswari, Ananganallur Nagarajan Shriram, Kishan Hari K. Raju, Ashwani Kumar Pathogens.2023; 12(12): 1406. CrossRef Microfilaria Positification Test Using Real-Time PCR Technique with HRM (High-Resolution Melting) Bagus Muhammad Ihsan, Afiat Berbudi, Ridad Agoes, Muhammad Ifham Hanif Acta Parasitologica.2022; 67(1): 496. CrossRef Collection and DNA Detection of Dirofilaria immitis (Rhabditida Onchocercidae), Using a Novel Primer Set, in Wild-Caught Mosquitoes From Gainesville, FL Chris Holderman, Nicole O Abruzzo, Noor A Abdelsamad, Phillip E Kaufman, Peter M DiGennaro, Stephen Rich Journal of Medical Entomology.2021; 58(3): 1429. CrossRef The use of molecular xenomonitoring for surveillance of mosquito-borne diseases Mary M. Cameron, Anita Ramesh Philosophical Transactions of the Royal Society B: Biological Sciences.2021; 376(1818): 20190816. CrossRef Canine and feline vector-borne diseases of zoonotic concern in Southeast Asia Viet-Linh Nguyen, Filipe Dantas-Torres, Domenico Otranto Current Research in Parasitology & Vector-Borne Diseases.2021; 1: 100001. CrossRef The Incidence of Dirofilaria immitis in Shelter Dogs and Mosquitoes in Austria Karin Sonnberger, Hans-Peter Fuehrer, Bernhard Werner Sonnberger, Michael Leschnik Pathogens.2021; 10(5): 550. CrossRef Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases Madeleine J. Rogers, Donald P. McManus, Stephen Muhi, Catherine A. Gordon Clinical Microbiology Reviews.2021;[Epub] CrossRef PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION Bagus Muhammad Ihsan, Cecep Dani Sucipto, Khayan Khayan Journal of Vocational Health Studies.2021; 5(1): 26. CrossRef Molecular detection of Trichostrongylus species through PCR followed by high resolution melt analysis of ITS-2 rDNA sequences Mohsen Arbabi, Hossein Hooshyar, Majid Lotfinia, Mohamad Ali Bakhshi Molecular and Biochemical Parasitology.2020; 236: 111260. CrossRef Prevalence and risk factors associated with Dirofilaria immitis infection in dogs and cats in Songkhla and Satun provinces, Thailand Ketsarin Kamyingkird, Witchuta Junsiri, Witsanuwat Chimnoi, Chanya Kengradomkij, Sinsamut Saengow, Keplee Sangchuto, Wicha Kajeerum, Decha Pangjai, Burin Nimsuphan, Tawin Inpankeaw, Sathaporn Jittapalapong Agriculture and Natural Resources.2017; 51(4): 299. CrossRef Is molecular xenomonitoring of mosquitoes for Dirofilaria repens suitable for dirofilariosis surveillance in endemic regions? Aleksander Masny, Rusłan Sałamatin, Wioletta Rozej-Bielicka, Elzbieta Golab Parasitology Research.2016; 115(2): 511. CrossRef Pyrosequencing Using SL and 5S rRNA as Molecular Markers for Identifying Zoonotic Filarial Nematodes in Blood Samples and Mosquitoes Oranuch Sanpool, Chairat Tantrawatpan, Tongjit Thanchomnang, Penchom Janwan, Pewpan M. Intapan, Rutchanee Rodpai, Viraphong Lulitanond, Piyanan Taweethavonsawat, Wanchai Maleewong Vector-Borne and Zoonotic Diseases.2016; 16(5): 326. CrossRef Detection and quantification of Wuchereria bancrofti and Brugia malayi DNA in blood samples and mosquitoes using duplex droplet digital polymerase chain reaction Jurairat Jongthawin, Pewpan M. Intapan, Viraphong Lulitanond, Oranuch Sanpool, Tongjit Thanchomnang, Lakkhana Sadaow, Wanchai Maleewong Parasitology Research.2016; 115(8): 2967. CrossRef Trypanosome infection rates in tsetse flies in the “silent” sleeping sickness focus of Bafia in the Centre Region in Cameroon Gustave Simo, Pierre Fongho, Oumarou Farikou, Prosper Innocent Ndjeuto Ndjeuto-Tchouli, Judith Tchouomene-Labou, Flobert Njiokou, Tazoacha Asonganyi Parasites & Vectors.2015;[Epub] CrossRef 12,262 View 139 Download Crossref Molecular Differentiation of Opisthorchis viverrini and Clonorchis sinensis Eggs by Multiplex Real-Time PCR with High Resolution Melting Analysis Worasak Kaewkong, Pewpan M. Intapan, Oranuch Sanpool, Penchom Janwan, Tongjit Thanchomnang, Porntip Laummaunwai, Viraphong Lulitanond, Pham Ngoc Doanh, Wanchai Maleewong Korean J Parasitol 2013;51(6):689-694. Published online December 31, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.6.689 Abstract PDF Opisthorchis viverrini and Clonorchis sinensis are parasites known to be carcinogenic and causative agents of cholangiocarcinoma in Asia. The standard method for diagnosis for those parasite infections is stool examination to detect parasite eggs. However, the method has low sensitivity, and eggs of O. viverrini and C. sinensis are difficult to distinguish from each other and from those of some other trematodes. Here, we report a multiplex real-time PCR coupled with high resolution melting (HRM) analysis for the differentiation of O. viverrini and C. sinensis eggs in fecal samples. Using 2 pairs of species-specific primers, DNA sequences from a portion of the mitochondrial NADH dehydrogenase subunit 2 (nad 2) gene, were amplified to generate 209 and 165 bp products for O. viverrini and C. sinensis, respectively. The distinct characteristics of HRM patterns were analyzed, and the melting temperatures peaked at 82.4±0.09℃ and 85.9±0.08℃ for O. viverrini and C. sinensis, respectively. This technique was able to detect as few as 1 egg of O. viverrini and 2 eggs of C. sinensis in a 150 mg fecal sample, which is equivalent to 7 and 14 eggs per gram of feces, respectively. The method is species-specific, rapid, simple, and does not require fluorescent probes or post-PCR processing for discrimination of eggs of the 2 species. It offers a new tool for differentiation and detection of Asian liver fluke infections in stool specimens. Citations Citations to this article as recorded by Multiparasitism in Spain in a Korean Visiting Friends and Relatives: Case Report and Review of Imported Cases of Clonorchis sinensis in Europe Paola Cociancic, Jacklyn Comas, J. Guillermo Esteban Clinical Case Reports.2025;[Epub] CrossRef Study of the population genetic structure of Opisthorchis-like eggs in northern Thailand using mitochondrial genes Picha Suwannahitatorn, Mathirut Mungthin, Ittisak Subrungruang, Lakhanawan Charoensuk, Nithikoon Aksorn, Saiwasan Buathong, Krystyna Cwiklinski PLOS Neglected Tropical Diseases.2024; 18(8): e0012445. CrossRef An Approach for Egg Parasite Classification Based on Ensemble Deep Learning Narut Butploy, Wanida Kanarkard, Pewpan M. Intapan, Oranuch Sanpool Journal of Advanced Computational Intelligence and Intelligent Informatics.2023; 27(6): 1113. CrossRef Fish and Food-Fatale: Food-borne Trematode Opisthorchis viverrini and Cholangiocarcinoma S. Tan, M. Machrumnizar Helminthologia.2023; 60(4): 287. CrossRef Are Melanoides tuberculata and Tarebia granifera (Gastropoda, Thiaridae), suitable first intermediate hosts of Clonorchis sinensis in Vietnam? Hung Manh Nguyen, Hien Hoang Van, Loan Thi Ho, Yulia V. Tatonova, Henry Madsen, Xiao-Nong Zhou PLOS Neglected Tropical Diseases.2021; 15(1): e0009093. CrossRef Clonorchis sinensis and clonorchiasis Byoung-Kuk Na, Jhang Ho Pak, Sung-Jong Hong Acta Tropica.2020; 203: 105309. CrossRef Serodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics Pyo Yun Cho, Ji-Yun Lee, Tae Im Kim, Jin-Ho Song, Sung-Jong Hong, Won Gi Yoo, Takafumi Tsuboi, Kwon-Soo Ha, Jae-Wan Jung, Satoru Takeo, Eun-Taek Han, Banchob Sripa, Sung-Tae Hong, Jong-Yil Chai, Ho-Woo Nam, Jhang Ho Pak, Tong-Soo Kim, Krystyna Cwiklinski PLOS Neglected Tropical Diseases.2020; 14(12): e0008998. CrossRef Clonorchiasis sinensis detected by laparoscopic exploration of biliary tracts in two patients with obstructive jaundice Xialei Liu, Genglong Zhu, Chaonong Cai, Zhiyue Lv, Jian Li BMC Infectious Diseases.2019;[Epub] CrossRef Development of Conventional Multiplex PCR: A Rapid Technique for Simultaneous Detection of Soil-Transmitted Helminths Vivornpun Sanprasert, Ruthairat Kerdkaew, Siriporn Srirungruang, Sarit Charuchaibovorn, Kobpat Phadungsaksawasdi, Surang Nuchprayoon Pathogens.2019; 8(3): 152. CrossRef Performance evaluation of existing immunoassays for Clonorchis sinensis infection in China Hong-Mei Li, Men-Bao Qian, Yi-Chao Yang, Zhi-Hua Jiang, Kang Wei, Jia-Xu Chen, Jun-Hu Chen, Ying-Dan Chen, Xiao-Nong Zhou Parasites & Vectors.2018;[Epub] CrossRef Molecular characterization of the unique Mesostephanus appendiculatus (Trematoda: Cyathocotylidae) by small ribosomal RNA from Egypt Nasr M. El-Bahy, Eman K. Bazh, Shimaa S. Sorour, Nagwa M. Elhawary Parasitology Research.2017; 116(4): 1129. CrossRef LAMPhimerus: A novel LAMP assay for detecting Amphimerus sp. DNA in human stool samples William Cevallos, Pedro Fernández-Soto, Manuel Calvopiña, Cristina Fontecha-Cuenca, Hiromu Sugiyama, Megumi Sato, Julio López Abán, Belén Vicente, Antonio Muro, David Blair PLOS Neglected Tropical Diseases.2017; 11(6): e0005672. CrossRef Molecular discrimination of Opisthorchis-like eggs from residents in a rural community of central Thailand Saiwasan Buathong, Saovanee Leelayoova, Mathirut Mungthin, Toon Ruang-areerate, Tawee Naaglor, Picha Suwannahitatorn, Phunlerd Piyaraj, Paanjit Taamasri, Peerapan Tan-ariya, Edoardo Pozio PLOS Neglected Tropical Diseases.2017; 11(11): e0006030. CrossRef Current status ofClonorchis sinensisand clonorchiasis in China De-Hua Lai, Xiao-Kun Hong, Bi-Xiu Su, Chi Liang, Geoff Hide, Xiaoli Zhang, Xinbing Yu, Zhao-Rong Lun Transactions of The Royal Society of Tropical Medicine and Hygiene.2016; 110(1): 21. CrossRef 11,355 View 131 Download Crossref A Recombinant Matrix Metalloproteinase Protein from Gnathostoma spinigerum for Serodiagnosis of Neurognathostomiasis Penchom Janwan, Pewpan M. Intapan, Hiroshi Yamasaki, Porntip Laummaunwai, Kittisak Sawanyawisuth, Chaisiri Wongkham, Chatchai Tayapiwatana, Amnat Kitkhuandee, Viraphong Lulitanond, Yukifumi Nawa, Wanchai Maleewong Korean J Parasitol 2013;51(6):751-754. Published online December 31, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.6.751 Abstract PDF Neurognathostomiasis is a severe form of human gnathostomiasis which can lead to disease and death. Diagnosis of neurognathostomiasis is made presumptively by using clinical manifestations. Immunoblotting, which recognizes antigenic components of molecular mass 21 kDa and 24 kDa in larval extracts of Gnathostoma spinigerum (Gs 21/24), has high sensitivity and specificity for diagnosis of neurognathostomiasis. However, only very small amounts of the Gs 21/24 antigens can be prepared from parasites harvested from natural or experimental animals. To overcome this problem, we recently produced a recombinant matrix metalloproteinase (rMMP) protein from G. spinigerum. In this study, we evaluated this rMMP alongside the Gs 21/24 antigens for serodiagnosis of human neurognathostomiasis. We studied sera from 40 patients from Srinagarind Hospital, Khon Kaen University, Thailand, with clinical criteria consistent with those of neurognathostomiasis, and sera from 30 healthy control adults from Thailand. All sera were tested for specific IgG antibodies against both G. spinigerum crude larval extract and rMMP protein using immunoblot analysis. The sensitivity and specificity for both antigenic preparations were all 100%. These results show that G. spinigerum rMMP protein can be used as an alternative diagnostic antigen, in place of larval extract, for serodiagnosis of neurognathostomiasis. Citations Citations to this article as recorded by Protein and antigen profiles of third-stage larvae of Gnathostoma spinigerum assessed with next-generation sequencing transcriptomic information Kathyleen Nogrado, Tipparat Thiangtrongjit, Poom Adisakwattana, Paron Dekumyoy, Sant Muangnoicharoen, Charin Thawornkuno, Onrapak Reamtong Scientific Reports.2022;[Epub] CrossRef Development of Immunochromatographic Test Kit for Rapid Detection of Specific IgG4 Antibody in Whole-Blood Samples for Diagnosis of Human Gnathostomiasis Penchom Janwan, Pewpan M. Intapan, Lakkhana Sadaow, Rutchanee Rodpai, Hiroshi Yamasaki, Patcharaporn Boonroumkaew, Oranuch Sanpool, Tongjit Thanchomnang, Phuangphaka Sadee, Wanchai Maleewong Diagnostics.2021; 11(5): 862. CrossRef Proteomics of Gnathostomiasis: A Way Forward for Diagnosis and Treatment Development Tipparat Thiangtrongjit, Kathyleen Nogrado, Thawatchai Ketboonlue, Preeyarat Malaitong, Poom Adisakwattana, Onrapak Reamtong Pathogens.2021; 10(9): 1080. CrossRef Combining lexical and context features for automatic ontology extension Sara Althubaiti, Şenay Kafkas, Marwa Abdelhakim, Robert Hoehndorf Journal of Biomedical Semantics.2020;[Epub] CrossRef Human gnathostomiasis: a neglected food-borne zoonosis Guo-Hua Liu, Miao-Miao Sun, Hany M. Elsheikha, Yi-Tian Fu, Hiromu Sugiyama, Katsuhiko Ando, Woon-Mok Sohn, Xing-Quan Zhu, Chaoqun Yao Parasites & Vectors.2020;[Epub] CrossRef Surveillance and diagnosis of zoonotic foodborne parasites Reza Zolfaghari Emameh, Sami Purmonen, Antti Sukura, Seppo Parkkila Food Science & Nutrition.2018; 6(1): 3. CrossRef 9,295 View 103 Download Crossref