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Yeon-Chul Hong	10 Articles
Prevalence of Trichomoniasis by PCR in Women Attending Health Screening in Korea Seung-Ryong Kim, Jung-Hyun Kim, Na-Yeong Gu, Yong-Suk Kim, Yeon-Chul Hong, Jae-Sook Ryu Korean J Parasitol 2016;54(2):187-190. Published online April 30, 2016 DOI: https://doi.org/10.3347/kjp.2016.54.2.187 Abstract PDF Trichomoniasis is the most common curable sexually-transmitted infection (STI) worldwide. There are few reports on the prevalence of Trichomonas vaginalis in Korea. The purpose of this study was to examine the prevalence of trichomoniasis by PCR in Guri city, Korea. All adult women who visited Hanyang University Guri Hospital for health screening within the National Health Care Service were invited to participate in the study, and 424 women were enrolled between March and June 2011. PCR was used to detect Trichomonas vaginalis using primers based on a repetitive sequence cloned from T. vaginalis (TV-E650). Fourteen women (3.3%) were found to have T. vaginalis. All were over 50, and they were significantly older on average than the 410 Trichomonas-negative women (mean ages 63.4 vs 55.3 years). It seems that T. vaginalis infection is not rare in women receiving health screening, especially among those over 50. Citations Citations to this article as recorded by Molecular diagnosis of Trichomonas vaginalis in liquid-based Papanicolaou samples in Shiraz, southern Iran Mohammad Saleh Bahreini, Samaneh Sedghi, Yalda Badalzadeh, Mohammad Hossein Motazedian, Manouchehr Shirani, Sareh Sami Jahromi, Aref Teimouri, Mahmoud Agholi, Qasem Asgari BMC Women's Health.2023;[Epub] CrossRef Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene Fakun Li, Yangyang Deng, Wanxin Sheng, Xihui Gao, Weijuan Wang, Zhili Chu, Xuefang Mei, Zhenke Yang, Xiaowei Tian, Shuai Wang, Zhenchao Zhang Journal of Eukaryotic Microbiology.2023;[Epub] CrossRef Molecular characterization of Trichomonas infections in women of Ilam City, southwestern Iran Maryam Alikhani, Mohammad Akhoundi, Denis Sereno, Jahangir Abdi, Razi Naserifar, Mohammad Reza Mahmoudi, Asad Mirzaei Parasitology Research.2022; 121(6): 1631. CrossRef Status of common parasitic diseases in Korea in 2019 Sun Huh Journal of the Korean Medical Association.2019; 62(8): 437. CrossRef 8,873 View 105 Download 5 Web of Science Crossref Construction of EST Database for Comparative Gene Studies of Acanthamoeba Eun-Kyung Moon, Joung-Ok Kim, Ying-Hua Xuan, Young-Sun Yun, Se Won Kang, Yong Seok Lee, Tae-In Ahn, Yeon-Chul Hong, Dong-Il Chung, Hyun-Hee Kong Korean J Parasitol 2009;47(2):103-107. Published online May 26, 2009 DOI: https://doi.org/10.3347/kjp.2009.47.2.103 Abstract PDF The genus Acanthamoeba can cause severe infections such as granulomatous amebic encephalitis and amebic keratitis in humans. However, little genomic information of Acanthamoeba has been reported. Here, we constructed Acanthamoeba expressed sequence tags (EST) database (Acanthamoeba EST DB) derived from our 4 kinds of Acanthamoeba cDNA library. The Acanthamoeba EST DB contains 3,897 EST generated from amebae under various conditions of long term in vitro culture, mouse brain passage, or encystation, and downloaded data of Acanthamoeba from National Center for Biotechnology Information (NCBI) and Taxonomically Broad EST Database (TBestDB). The almost reported cDNA/genomic sequences of Acanthamoeba provide stand alone BLAST system with nucleotide (BLAST NT) and amino acid (BLAST AA) sequence database. In BLAST results, each gene links for the significant information including sequence data, gene orthology annotations, relevant references, and a BlastX result. This is the first attempt for construction of Acanthamoeba database with genes expressed in diverse conditions. These data were integrated into a database (http://www.amoeba.or.kr). Citations Citations to this article as recorded by High-efficiency transfection of Acanthamoeba castellanii using a cationic polymer Anaísa B. Moreno, Viktor Ek, Jens Eriksson, Mikael E. Sellin, Lionel Guy Cell Reports Methods.2026; : 101269. CrossRef Recombinant protein expression in Acanthamoeba castellanii Pooja Salunke, Kiran Kondabagil, Yogesh A. Karpe Frontiers in Bioengineering and Biotechnology.2025;[Epub] CrossRef Biological characteristics and pathogenicity of Acanthamoeba Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li Frontiers in Microbiology.2023;[Epub] CrossRef Autophagy protein 12 plays an essential role in Acanthamoeba encystation So-Hee Kim, Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Hyun-Hee Kong Experimental Parasitology.2015; 159: 46. CrossRef Caspase-like proteins: Acanthamoeba castellanii metacaspase and Dictyostelium discoideum paracaspase, what are their functions? Entsar Saheb, Wendy Trzyna, John Bush Journal of Biosciences.2014; 39(5): 909. CrossRef Helminth parasites of fish and shellfish from the Santa Gilla Lagoon in southern Sardinia, Italy J. Culurgioni, A. Sabatini, R. De Murtas, S. Mattiucci, V. Figus Journal of Helminthology.2014; 88(04): 489. CrossRef Microarray and KOG analysis of Acanthamoeba healyi genes up-regulated by mouse-brain passage Eun-Kyung Moon, Ying-Hua Xuan, Hyun-Hee Kong Experimental Parasitology.2014; 143: 69. CrossRef Protein kinase C signaling molecules regulate encystation of Acanthamoeba Eun-Kyung Moon, Dong-Il Chung, Yeonchul Hong, Hyun-Hee Kong Experimental Parasitology.2012; 132(4): 524. CrossRef Microarray Analysis of Differentially Expressed Genes between Cysts and Trophozoites ofAcanthamoeba castellanii Eun-Kyung Moon, Ying-Hua Xuan, Dong-Il Chung, Yeonchul Hong, Hyun-Hee Kong The Korean Journal of Parasitology.2011; 49(4): 341. CrossRef Drug target identification, validation, characterisation and exploitation for treatment of Acanthamoeba (species) infections Craig W. Roberts, Fiona L. Henriquez Experimental Parasitology.2010; 126(1): 91. CrossRef Construction of Web-Based Database for Anisakis Research Yong-Seok Lee, Moon-Ki Baek, Yong-Hun Jo, Se-Won Kang, Jae-Bong Lee, Yeon-Soo Han, Hee-Jae Cha, Hak-Sun Yu, Mee-Sun Ock Journal of Life Science.2010; 20(3): 411. CrossRef 10,438 View 59 Download Crossref Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung Korean J Parasitol 2008;46(3):157-164. Published online September 20, 2008 DOI: https://doi.org/10.3347/kjp.2008.46.3.157 Abstract PDF Three Acanthamoeba isolates (KA/E9, KA/E17, and KA/E23) from patients with keratitis were identified as Acanthamoeba triangularis by analysis of their molecular characteristics, a species not previously recognized to be a corneal pathogen. Epidemiologic significance of A. triangularis as a keratopathogen in Korea has been discussed. Morphologic features of Acanthamoeba cysts were examined under a microscope with differential interference contrast (DIC) optics. Mitochondrial DNA (mtDNA) of the ocular isolates KA/E9, KA/E17, and KA/E23 were digested with restriction enzymes, and the restriction patterns were compared with those of reference strains. Complete nuclear 18S and mitochondrial (mt) 16S rDNA sequences were subjected to phylogenetic analysis and species identification. mtDNA RFLP of 3 isolates showed very similar patterns to those of SH621, the type strain of A. triangularis. 16S and 18S rDNA sequence analysis confirmed 3 isolates to be A. triangularis. 18S rDNA sequence differences of the isolates were 1.3% to 1.6% and those of 16S rDNA, 0.4% to 0.9% from A. triangularis SH621. To the best of our knowledge, this is the first report, confirmed by 18S and 16S rDNA sequence analysis, of keratitis caused by A. triangularis of which the type strain was isolated from human feces. Six isolates of A. triangularis had been reported from contaminated contact lens cases in south eastern Korea. Citations Citations to this article as recorded by Inhibitory and anti-adherent effects of Piper betle L. leaf extract against Acanthamoeba triangularis in co-infection with Staphylococcus aureus and Pseudomonas aeruginosa: A sustainable one-health approach Pattamaporn Kwankaew, Suthinee Sangkanu, Watcharapong Mitsuwan, Rachasak Boonhok, Udom Lao-On, Hazel L. Tabo, Tooba Mahboob, Maria de Lourdes Pereira, Jitbanjong Tangpong, Shanmuga S. Sundar, Christophe Wiart, Veeranoot Nissapatorn Veterinary World.2024; : 848. CrossRef The Status of Molecular Analyses of Isolates of Acanthamoeba Maintained by International Culture Collections Paul A. Fuerst Microorganisms.2023; 11(2): 295. CrossRef Sub-Genotyping of Acanthamoeba T4 Complex: Experience from North India Kirti Megha, Megha Sharma, Amit Gupta, Rakesh Sehgal, Sumeeta Khurana Parasitologia.2023; 3(1): 69. CrossRef The First Case of Cutaneous Acanthamoebiasis Caused by Acanthamoeba triangularis in Korea Mi Soo Choi, Na Hye Myong, Min Seo, Sukbin Jang, Dae Kwan Yun, Kyujin Yeom, Dong-Il Chung, Byung Cheol Park, Yeonchul Hong, Myung Hwa Kim Annals of Dermatology.2023; 35(Suppl 2): S275. CrossRef Detection of Acanthamoeba spp. using carboxylesterase antibody and its usage for diagnosing Acanthamoeba-keratitis Min-Jeong Kim, Ki-Back Chu, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon, Ashok Kumar PLOS ONE.2022; 17(1): e0262223. CrossRef Report of rare genotypes of Acanthamoeba from soil source of the Payeh Maga Highland forest, North-eastern Sarawak, Malaysia Li-Li Chan, Hiu-Ching Toh, Kantiya Jaikwang, Ee-Mun Loo, Jia-Haw Wong, Yun-Khoon Liew, Kian-Huat Ong, Soo-Shen Ooi Acta Tropica.2022; 229: 106372. CrossRef Isolation and morphological and molecular characterization of waterborne free-living amoebae: Evidence of potentially pathogenic Acanthamoeba and Vahlkampfiidae in Assiut, Upper Egypt Martina M. Nageeb, Hanan E. M. Eldeek, Rasha A. H. Attia, Atef A. Sakla, Samia S. Alkhalil, Haiam Mohamed Mahmoud Farrag, Ebrahim Shokoohi PLOS ONE.2022; 17(7): e0267591. CrossRef An Insight into the Genome of Pathogenic and Non-Pathogenic Acanthamoeba Chayan Sharma, Sumeeta Khurana, Amit Arora, Alka Bhatia, Amit Gupta Pathogens.2022; 11(12): 1558. CrossRef Orthokeratology lens-related Acanthamoeba keratitis: case report and analytical review Jinfang Wu, Huatao Xie Journal of International Medical Research.2021;[Epub] CrossRef Anti-Acanthamoeba synergistic effect of chlorhexidine and Garcinia mangostana extract or α-mangostin against Acanthamoeba triangularis trophozoite and cyst forms Suthinee Sangkanu, Watcharapong Mitsuwan, Wilawan Mahabusarakam, Tajudeen O. Jimoh, Polrat Wilairatana, Ana Paula Girol, Ajoy K. Verma, Maria de Lourdes Pereira, Mohammed Rahmatullah, Christophe Wiart, Abolghasem Siyadatpanah, Roghayeh Norouzi, Polydor Ng Scientific Reports.2021;[Epub] CrossRef Amoebicidal activity of Cassia angustifolia extract and its effect on Acanthamoeba triangularis autophagy-related gene expression at the transcriptional level Rachasak Boonhok, Suthinee Sangkanu, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Nurdina Charong, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Hazel Parasitology.2021; 148(9): 1074. CrossRef Peganum harmala Extract Has Antiamoebic Activity to Acanthamoeba triangularis Trophozoites and Changes Expression of Autophagy-Related Genes Rachasak Boonhok, Suthinee Sangkanu, Julalak Chuprom, Mayuna Srisuphanunt, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Chr Pathogens.2021; 10(7): 842. CrossRef Molecular detection of free-living amoebae from Namhangang (southern Han River) in Korea Heekyoung Kang, Hae-Jin Sohn, Ga-Eun Seo, Gi-Sang Seong, A-Jeong Ham, A-Young Park, Suk-Yul Jung, Sang-Eun Lee, Shin-Hyeong Cho, Ho-Joon Shin Scientific Reports.2020;[Epub] CrossRef Description of Virulent Factors and Horizontal Gene Transfers of Keratitis-Associated Amoeba Acanthamoeba Triangularis by Genome Analysis Issam Hasni, Julien Andréani, Philippe Colson, Bernard La Scola Pathogens.2020; 9(3): 217. CrossRef Update on Acanthamoeba phylogeny Daniele Corsaro Parasitology Research.2020; 119(10): 3327. CrossRef Production of a polyclonal antibody against inosine-uridine preferring nucleoside hydrolase of Acanthamoeba castellanii and its access to diagnosis of Acanthamoeba keratitis So-Min Park, Hae-Ahm Lee, Ki-Back Chu, Fu-Shi Quan, Su-Jung Kim, Eun-Kyung Moon, Paulo Lee Ho PLOS ONE.2020; 15(9): e0239867. CrossRef Review of Successful Control of Parasitic Infections in Korea Sung-Tae Hong, Tai-Soon Yong Infection & Chemotherapy.2020; 52(3): 427. CrossRef The Spectrum of Microbial Keratitis: An Updated Review Christopher Bartimote, John Foster, Stephanie Watson The Open Ophthalmology Journal.2019; 13(1): 100. CrossRef Pathogenic waterborne free-living amoebae: An update from selected Southeast Asian countries Mohamad Azlan Abdul Majid, Tooba Mahboob, Brandon G. J. Mong, Narong Jaturas, Reena Leeba Richard, Tan Tian-Chye, Anusorn Phimphila, Panomphanh Mahaphonh, Kyaw Nyein Aye, Wai Lynn Aung, Joon Chuah, Alan D. Ziegler, Atipat Yasiri, Nongyao Sawangjaroen, Yvo PLOS ONE.2017; 12(2): e0169448. CrossRef Isolation and identification of Acanthamoeba strains from soil and tap water in Yanji, China Yinghua Xuan, Yanqin Shen, Yuxi Ge, Gen Yan, Shanzi Zheng Environmental Health and Preventive Medicine.2017;[Epub] CrossRef Acanthamoebicidal activity of periglaucine A and betulinic acid from Pericampylus glaucus (Lam.) Merr. in vitro Tooba Mahboob, Abdul-Majid Azlan, Fiona Natalia Shipton, Patcharaporn Boonroumkaew, Nadiah Syafiqah Nor Azman, Shamala Devi Sekaran, Init Ithoi, Tian-Chye Tan, Chandramathi Samudi, Christophe Wiart, Veeranoot Nissapatorn Experimental Parasitology.2017; 183: 160. CrossRef Anti-encystment and amoebicidal activity of Lonicera japonica Thunb. and its major constituent chlorogenic acid in vitro Tooba Mahboob, Abdul-Majid Azlan, Tian-Chye Tan, Chandramathi Samudi, Shamala Devi Sekaran, Veeranoot Nissapatorn, Christophe Wiart Asian Pacific Journal of Tropical Medicine.2016; 9(9): 866. CrossRef First report of an Acanthamoeba genotype T13 isolate as etiological agent of a keratitis in humans Anna-Lena Grün, Birthe Stemplewitz, Patrick Scheid Parasitology Research.2014; 113(6): 2395. CrossRef Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Acanthamoeba Hye-Won Yang, Yu-Ran Lee, Noboru Inoue, Bijay Kumar Jha, Dinzouna-Boutamba Sylvatrie Danne, Hong-Kyun Kim, Junhun Lee, Youn-Kyoung Goo, Hyun-Hee Kong, Dong-Il Chung, Yeonchul Hong The Korean Journal of Parasitology.2013; 51(3): 269. CrossRef Imported Intraocular Gnathostomiasis with Subretinal Tracks Confirmed by Western Blot Assay Ji Ho Yang, Moosang Kim, Eung Suk Kim, Byoung-Kuk Na, Seung-Young Yu, Hyung-Woo Kwak The Korean Journal of Parasitology.2012; 50(1): 73. CrossRef Morphological, physiological and molecular biological characterisation of isolates from first cases of Acanthamoeba keratitis in Slovakia Viera Nagyová, Arpád Nagy, Jozef Timko Parasitology Research.2010; 106(4): 861. CrossRef Acanthamoeba strains show reduced temperature tolerance after long-term axenic culture Wilawan Pumidonming, Martina Koehsler, Julia Walochnik Parasitology Research.2010; 106(3): 553. CrossRef Molecular Phylogeny of Acanthamoeba Hyun Hee Kong The Korean Journal of Parasitology.2009; 47(Suppl): S21. CrossRef 10,263 View 88 Download Crossref Differentially expressed genes of Acanthamoeba castellanii during encystation Eun-Kyung Moon, Dong-Il Chung, Yeon-Chul Hong, Hyun-Hee Kong Korean J Parasitol 2007;45(4):283-285. Published online December 20, 2007 DOI: https://doi.org/10.3347/kjp.2007.45.4.283 Abstract PDF To examine the expressed gene profile during encystation of Acanthamoeba castellanii Castellani, we used differentially expressed gene (DGE) screening by RT-PCR with 20 sets of random primers. From this analysis, we found that approximately 16 genes showed upregulation during encystation. We chose 6 genes, which had relatively higher expression levels, for further investigation. Based on homology search in database, DEG2 showed 55% of similarity with xylose isomerase, DEG9 showed 37% of similarity with Na P-type ATPase, and DEG14 showed 77% of similarity with subtilisin-like serine proteinase. DEG3 and DEG26 were identified as hypothetical proteins and DEG25 exhibited no significant similarity to any known protein. Encystation of Acanthamoeba has been suggested to be a process to resist adverse environmental or nutritional conditions. Further characterization studies of these genes may provide us with more information on the encystation mechanism of Acanthamoeba. Citations Citations to this article as recorded by Encystment and Excystment Processes in Acanthamoeba castellanii: An Emphasis on Cellulose Involvement Mathew Choaji, Ascel Samba-Louaka, Zineb Fechtali-Moute, Willy Aucher, Sébastien Pomel Pathogens.2025; 14(3): 268. CrossRef Oxidase enzyme genes are differentially expressed during Acanthamoeba castellanii encystment Christian Q. Scheckhuber, Rebeca Damián Ferrara, Jesús Gómez-Montalvo, Sutherland K. Maciver, Alvaro de Obeso Fernández del Valle Parasitology Research.2024;[Epub] CrossRef Ouabain, ATPase inhibitor, potentially enhances the effect of polyhexamethylene biguanide on Acanthamoeba castellanii Kuang-Yi Shih, Yao-Tsung Chang, Yu-Jen Wang, Jian-Ming Huang International Journal for Parasitology: Drugs and Drug Resistance.2024; 25: 100550. CrossRef Acanthamoeba keratitis: new hopes for potential interventions for a curable but often refractory disease Bader Saleem Alawfi, Naveed Ahmed Khan, David Lloyd, Ruqaiyyah Siddiqui Expert Review of Ophthalmology.2024; 19(4): 271. CrossRef Biological characteristics and pathogenicity of Acanthamoeba Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li Frontiers in Microbiology.2023;[Epub] CrossRef mRNA Sequencing Reveals Upregulation of Glutathione S-Transferase Genes during Acanthamoeba Encystation Alvaro de Obeso Fernández del Valle, Christian Quintus Scheckhuber, David Armando Chavaro-Pérez, Erandi Ortega-Barragán, Sutherland K. Maciver Microorganisms.2023; 11(4): 992. CrossRef Stimulation of Acanthamoeba castellanii excystment by enzyme treatment and consequences on trophozoite growth Zineb Fechtali-Moute, Philippe M. Loiseau, Sébastien Pomel Frontiers in Cell and Developmental Biology.2022;[Epub] CrossRef Peganum harmala Extract Has Antiamoebic Activity to Acanthamoeba triangularis Trophozoites and Changes Expression of Autophagy-Related Genes Rachasak Boonhok, Suthinee Sangkanu, Julalak Chuprom, Mayuna Srisuphanunt, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Chr Pathogens.2021; 10(7): 842. CrossRef Whole Organism Model to Study Molecular Mechanisms of Differentiation and Dedifferentiation Areeba Anwar, Ruqaiyyah Siddiqui, Naveed Ahmed Khan Biology.2020; 9(4): 79. CrossRef New insights into the mechanical properties of Acanthamoeba castellanii cysts as revealed by phonon microscopy Fernando Pérez-Cota, Richard J. Smith, Hany M. Elsheikha, Matt Clark Biomedical Optics Express.2019; 10(5): 2399. CrossRef Encystation: the most prevalent and underinvestigated differentiation pathway of eukaryotes Pauline Schaap, Christina Schilde Microbiology.2018; 164(5): 727. CrossRef Acanthamoeba and mimivirus interactions: the role of amoebal encystment and the expansion of the ‘Cheshire Cat’ theory Ludmila Karen dos Santos Silva, Paulo Victor Miranda Boratto, Bernard La Scola, Cláudio Antônio Bonjardim, Jônatas Santos Abrahão Current Opinion in Microbiology.2016; 31: 9. CrossRef Autophagy protein 12 plays an essential role in Acanthamoeba encystation So-Hee Kim, Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Hyun-Hee Kong Experimental Parasitology.2015; 159: 46. CrossRef Down-Regulation of Cellulose Synthase Inhibits the Formation of Endocysts in Acanthamoeba Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong The Korean Journal of Parasitology.2014; 52(2): 131. CrossRef Chloroquine Has a Cytotoxic Effect on Acanthamoeba Encystation through Modulation of Autophagy Bijay Kumar Jha, Hui-Jung Jung, Incheol Seo, Hyun Ah Kim, Seong-Il Suh, Min-Ho Suh, Won-Ki Baek Antimicrobial Agents and Chemotherapy.2014; 58(10): 6235. CrossRef Silencing of xylose isomerase and cellulose synthase by siRNA inhibits encystation in Acanthamoeba castellanii Yousuf Aqeel, Ruqaiyyah Siddiqui, Naveed Ahmed Khan Parasitology Research.2013; 112(3): 1221. CrossRef Acanthamoebadifferentiation: a two-faced drama ofDr Jekyll and Mr Hyde RUQAIYYAH SIDDIQUI, RICKY DUDLEY, NAVEED AHMED KHAN Parasitology.2012; 139(7): 826. CrossRef Protein kinase C signaling molecules regulate encystation of Acanthamoeba Eun-Kyung Moon, Dong-Il Chung, Yeonchul Hong, Hyun-Hee Kong Experimental Parasitology.2012; 132(4): 524. CrossRef Cellular, Biochemical, and Molecular Changes during Encystment of Free-Living Amoebae Emilie Fouque, Marie-Cécile Trouilhé, Vincent Thomas, Philippe Hartemann, Marie-Hélène Rodier, Yann Héchard Eukaryotic Cell.2012; 11(4): 382. CrossRef Microarray Analysis of Differentially Expressed Genes between Cysts and Trophozoites ofAcanthamoeba castellanii Eun-Kyung Moon, Ying-Hua Xuan, Dong-Il Chung, Yeonchul Hong, Hyun-Hee Kong The Korean Journal of Parasitology.2011; 49(4): 341. CrossRef Major Role for Cysteine Proteases during the Early Phase of Acanthamoeba castellanii Encystment David Leitsch, Martina Köhsler, Martina Marchetti-Deschmann, Andrea Deutsch, Günter Allmaier, Michael Duchêne, Julia Walochnik Eukaryotic Cell.2010; 9(4): 611. CrossRef Acanthamoeba castellanii: Proteins involved in actin dynamics, glycolysis, and proteolysis are regulated during encystation Sabrina Bouyer, Marie-Hélène Rodier, Alain Guillot, Yann Héchard Experimental Parasitology.2009; 123(1): 90. CrossRef Characterization of a Serine Proteinase Mediating Encystation of Acanthamoeba Eun-Kyung Moon, Dong-Il Chung, Yeon-Chul Hong, Hyun-Hee Kong Eukaryotic Cell.2008; 7(9): 1513. CrossRef 8,532 View 123 Download Crossref Natural occurrence of Mycobacterium as an endosymbiont of Acanthamoeba isolated from a contact lens storage case Hak Sun Yu, Hae Jin Jeong, Yeon-Chul Hong, Seong-Yong Seol, Dong-Il Chung, Hyun-Hee Kong Korean J Parasitol 2007;45(1):11-18. Published online March 20, 2007 DOI: https://doi.org/10.3347/kjp.2007.45.1.11 Abstract PDF Recent in vitro studies have revealed that a certain Mycobacterium can survive and multiply within free-living amoebae. It is believed that protozoans function as host cells for the intracellular replication and evasion of Mycobacterium spp. under harmful conditions. In this study, we describe the isolation and characterization of a bacterium naturally observed within an amoeba isolate acquired from a contact lens storage case. The bacterium multiplied within Acanthamoeba, but exerted no cytopathic effects on the amoeba during a 6-year amoebic culture. Trasnmission electron microscopy showed that the bacteria were randomly distributed within the cytoplasm of trophozoites and cysts of Acanthamoeba. On the basis of the results of 18S rRNA gene analysis, the amoeba was identified as A. lugdunensis. A 16S rRNA gene analysis placed this bacterium within the genus Mycobacterium. The bacterium evidenced positive reactivity for acid-fast and fluorescent acid-fast stains. The bacterium was capable of growth on the Middlebrook 7H11-Mycobacterium-specific agar. The identification and characterization of bacterial endosymbionts of free-living protozoa bears significant implications for our understanding of the ecology and the identification of other atypical mycobacterial pathogens. Citations Citations to this article as recorded by A Comparative Genomic Approach to Determine the Virulence Factors and Horizontal Gene Transfer Events of Clinical Acanthamoeba Isolates Xiaobin Gu, Xiuhai Lu, Shudan Lin, Xinrui Shi, Yue Shen, Qingsong Lu, Yiying Yang, Jing Yang, Jiabei Cai, Chunyan Fu, Yongliang Lou, Meiqin Zheng, Tim Downing, Kirti Megha Microbiology Spectrum.2022;[Epub] CrossRef A Systematic Review of Intracellular Microorganisms within Acanthamoeba to Understand Potential Impact for Infection Binod Rayamajhee, Dinesh Subedi, Hari Kumar Peguda, Mark Duncan Willcox, Fiona L. Henriquez, Nicole Carnt Pathogens.2021; 10(2): 225. CrossRef Detection of Free-Living Amoebae and Their Intracellular Bacteria in Borehole Water before and after a Ceramic Pot Filter Point-of-Use Intervention in Rural Communities in South Africa Clarissa van der Loo, Catheleen Bartie, Tobias George Barnard, Natasha Potgieter International Journal of Environmental Research and Public Health.2021; 18(8): 3912. CrossRef The key factors contributing to the risk, diagnosis and treatment of non-tuberculous mycobacterial opportunistic infections Anna Grzegorzewicz, Mariola Paściak Postępy Higieny i Medycyny Doświadczalnej.2021; 75(1): 696. CrossRef Co-Existence of Free-Living Amoebae and Potential Human Pathogenic Bacteria Isolated from Rural Household Water Storage Containers Natasha Potgieter, Clarissa van der Loo, Tobias George Barnard Biology.2021; 10(12): 1228. CrossRef The core microbiome of sessile ciliate Stentor coeruleus is not shaped by the environment Olivia Lanzoni, Andrey Plotnikov, Yuri Khlopko, Giulio Munz, Giulio Petroni, Alexey Potekhin Scientific Reports.2019;[Epub] CrossRef Repertoire of free-living protozoa in contact lens solutions Ibtissem Bouchoucha, Aurore Aziz, Louis Hoffart, Michel Drancourt BMC Ophthalmology.2016;[Epub] CrossRef Current and Past Strategies for Bacterial Culture in Clinical Microbiology Jean-Christophe Lagier, Sophie Edouard, Isabelle Pagnier, Oleg Mediannikov, Michel Drancourt, Didier Raoult Clinical Microbiology Reviews.2015; 28(1): 208. CrossRef Looking in amoebae as a source of mycobacteria M. Drancourt Microbial Pathogenesis.2014; 77: 119. CrossRef Molecular characterization and ultrastructure of a new amoeba endoparasite belonging to the Stenotrophomonas maltophilia complex Daniele Corsaro, Karl-Dieter Müller, Rolf Michel Experimental Parasitology.2013; 133(4): 383. CrossRef Identification of Free-Living Amoebae and Amoeba-Associated Bacteria from Reservoirs and Water Treatment Plants by Molecular Techniques Alicia Garcia, Pilar Goñi, Joanna Cieloszyk, Maria Teresa Fernandez, Laura Calvo-Beguería, Encarnacion Rubio, Maria Francisca Fillat, Maria Luisa Peleato, Antonio Clavel Environmental Science & Technology.2013; 47(7): 3132. CrossRef Cooccurrence of Free-Living Amoebae and Nontuberculous Mycobacteria in Hospital Water Networks, and Preferential Growth of Mycobacterium avium in Acanthamoeba lenticulata Alida R. Ovrutsky, Edward D. Chan, Marinka Kartalija, Xiyuan Bai, Mary Jackson, Sara Gibbs, Joseph O. Falkinham, Michael D. Iseman, Paul R. Reynolds, Gerald McDonnell, Vincent Thomas Applied and Environmental Microbiology.2013; 79(10): 3185. CrossRef Detection of Bacterial Endosymbionts in Clinical Acanthamoeba Isolates Alfonso Iovieno, Dolena R. Ledee, Darlene Miller, Eduardo C. Alfonso Ophthalmology.2010; 117(3): 445. CrossRef Biodiversity of amoebae and amoeba-associated bacteria in water treatment plants Daniele Corsaro, Gemma Saucedo Pages, Vicente Catalan, Jean-François Loret, Gilbert Greub International Journal of Hygiene and Environmental Health.2010; 213(3): 158. CrossRef Free-living amoebae and their intracellular pathogenic microorganisms: risks for water quality Vincent Thomas, Gerald McDonnell, Stephen P. Denyer, Jean-Yves Maillard FEMS Microbiology Reviews.2010; 34(3): 231. CrossRef Free-living amoebae, a training field for macrophage resistance of mycobacteria I.B. Salah, E. Ghigo, M. Drancourt Clinical Microbiology and Infection.2009; 15(10): 894. CrossRef Endosymbionts of Acanthamoeba Isolated from Domestic Tap Water in Korea Seon Hee Choi, Min Kyoung Cho, Soon Cheol Ahn, Ji Eun Lee, Jong Soo Lee, Dong-Hee Kim, Ying-Hua Xuan, Yeon Chul Hong, Hyun Hee Kong, Dong Il Chung, Hak Sun Yu The Korean Journal of Parasitology.2009; 47(4): 337. CrossRef Survival of amoebae on building materials T. Yli-Pirilä, J. Kusnetsov, M.-R. Hirvonen, M. Seuri, A. Nevalainen Indoor Air.2009; 19(2): 113. CrossRef Occurrence of Free-Living Amoebae in Communities of Low and High Endemicity for Buruli Ulcer in Southern Benin Miriam Eddyani, Johan F. De Jonckheere, Lies Durnez, Patrick Suykerbuyk, Herwig Leirs, Françoise Portaels Applied and Environmental Microbiology.2008; 74(21): 6547. CrossRef Relationship between mycobacteria and amoebae: ecological and epidemiological concerns V. Thomas, G. McDonnell Letters in Applied Microbiology.2007; 45(4): 349. CrossRef 11,058 View 97 Download Crossref Molecular and biochemical characterization of a novel actin bundling protein in Acanthamoeba Joanna It-itan Alafag, Eun-Kyung Moon, Yeon-Chul Hong, Dong-Il Chung, Hyun-Hee Kong Korean J Parasitol 2006;44(4):331-341. Published online December 20, 2006 DOI: https://doi.org/10.3347/kjp.2006.44.4.331 Abstract PDF Actin binding proteins play key roles in cell structure and movement particularly as regulators of the assembly, stability and localization of actin filaments in the cytoplasm. In the present study, a cDNA clone encoding an actin bundling protein named as AhABP was isolated from Acanthamoeba healyi, a causative agent of granulomatous amebic encephalitis. This clone exhibited high similarity with genes of Physarum polycephalum and Dictyostelium discoideum, which encode actin bundling proteins. Domain search analysis revealed the presence of essential conserved regions, i.e., an active actin binding site and 2 putative calcium binding EF-hands. Transfected amoeba cells demonstrated that AhABP is primarily localized in phagocytic cups, peripheral edges, pseudopods, and in cortical cytoplasm where actins are most abundant. Moreover, AhABP after the deletion of essential regions formed ellipsoidal inclusions within transfected cells. High-speed co-sedimentation assays revealed that AhABP directly interacted with actin in the presence of up to 10 ?M of calcium. Under the electron microscope, thick parallel bundles were formed by full length AhABP, in contrast to the thin actin bundles formed by constructs with deletion sites. In the light of these results, we conclude that AhABP is a novel actin bundling protein that is importantly associated with actin filaments in the cytoplasm. Citations Citations to this article as recorded by GILT in tumor cells improves T cell-mediated anti-tumor immune surveillance Hongshuai Li, Yuan Wang, Mengchu Ma, Lihong Hu, Xinxin Zhang, Lingbiao Xin, Wei Zhang, Xiaoming Sun, Yuanyuan Ren, Xinting Wang, Jie Yang Immunology Letters.2021; 234: 1. CrossRef Acanthamoeba castellanii cysts: new ultrastructural findings Bibiana Chávez-Munguía, Lizbeth Salazar-Villatoro, Anel Lagunes-Guillén, Maritza Omaña-Molina, Martha Espinosa-Cantellano, Adolfo Martínez-Palomo Parasitology Research.2013; 112(3): 1125. CrossRef In Vitro Efficacies of Clinically Available Drugs against Growth and Viability of an Acanthamoeba castellanii Keratitis Isolate Belonging to the T4 Genotype Abdul Mannan Baig, Junaid Iqbal, Naveed Ahmed Khan Antimicrobial Agents and Chemotherapy.2013; 57(8): 3561. CrossRef 9,879 View 57 Download Crossref Comparison of specific activity and cytopathic effects of purified 33 kDa serine proteinase from Acanthamoeba strains with different degree of virulence Won-Tae Kim, Hyun-Hee Kong, Young-Ran Ha, Yeon-Chul Hong, Hae Jin Jeong, Hak Sun Yu, Dong-Il Chung Korean J Parasitol 2006;44(4):321-330. Published online December 20, 2006 DOI: https://doi.org/10.3347/kjp.2006.44.4.321 Abstract PDF The pathogenic mechanism of granulomatous amebic encephalitis (GAE) and amebic keratitis (AK) by Acanthamoeba has yet to be clarified. Protease has been recognized to play an important role in the pathogenesis of GAE and AK. In the present study, we have compared specific activity and cytopathic effects (CPE) of purified 33 kDa serine proteinases from Acanthamoeba strains with different degree of virulence (A. healyi OC-3A, A. lugdunensis KA/E2, and A. castellanii Neff). Trophozoites of the 3 strains revealed different degrees of CPE on human corneal epithelial (HCE) cells. The effect was remarkably reduced by adding phenylmethylsulfonylfluoride (PMSF), a serine proteinase inhibitor. This result indicated that PMSF-susceptible proteinase is the main component causing cytopathy to HCE cells by Acanthamoeba. The purified 33 kDa serine proteinase showed strong activity toward HCE cells and extracellular matrix proteins. The purified proteinase from OC-3A, the most virulent strain, demonstrated the highest enzyme activity compared to KA/E2, an ocular isolate, and Neff, a soil isolate. Polyclonal antibodies against the purified 33 kDa serine proteinase inhibit almost completely the proteolytic activity of culture supernatant of Acanthamoeba. In line with these results, the 33 kDa serine proteinase is suggested to play an important role in pathogenesis and to be the main component of virulence factor of Acanthamoeba. Citations Citations to this article as recorded by Calcium ions in tap water may increase the adhesion ability of Acanthamoeba, potentially enhancing its cytopathic effects on corneal cells Yu-Jen Wang, Yao-Tsung Chang, Tsun-Hsien Hsiao, Chun-Hsien Chen, Chih-Ming Tsai, Jian-Ming Huang Parasite.2025; 32: 71. CrossRef Proteases of Acanthamoeba Behroz Mahdavi Poor, Jalil Rashedi, Vahid Asgharzadeh, Amirali Mirmazhary, Nazila Gheitarani Parasitology Research.2024;[Epub] CrossRef Characterization of novel extracellular proteases produced by Acanthamoeba castellanii after contact with human corneal epithelial cells and their relevance to pathogenesis Alvie Loufouma-Mbouaka, Tania Martín-Pérez, Martina Köhsler, Zeynep Danisman, Maya Schwarz, Rounik Mazumdar, Ascel Samba-Louaka, Julia Walochnik Parasites & Vectors.2024;[Epub] CrossRef Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells Abdullah Alhazmi, Laura E. Sidney, Andy Hopkinson, Hany M. Elsheikha Acta Tropica.2024; 257: 107288. CrossRef Assessment of in vitro dynamics of pathogenic environmental Acanthamoeba T4 and T9 genotypes isolated from three recreational lakes in Klang Valley, Malaysia over the HaCaT cell monolayer Rohaya Abdul Halim, Hasseri Halim, Rosnani Hanim Mohd Hussain, Shafiq Aazmi, Naveed Ahmed Khan, Ruqaiyyah Siddiqui, Tengku Shahrul Anuar Journal of Water and Health.2024; 22(12): 2289. CrossRef Identification of an Antimicrobial Protease from Acanthamoeba via a Novel Zymogram Alvaro de Obeso Fernández del Valle, Luis Javier Melgoza-Ramírez, María Fernanda Esqueda Hernández, Alfonso David Rios-Pérez, Sutherland K. Maciver Processes.2023; 11(9): 2620. CrossRef Acanthamoeba Mannose and Laminin Binding Proteins Variation across Species and Genotypes Daniele Corsaro Microorganisms.2022; 10(11): 2162. CrossRef In Vitro Cytopathogenic Activities of Acanthamoeba T3 and T4 Genotypes on HeLa Cell Monolayer Rosnani Hanim Mohd Hussain, Mohamed Kamel Abdul Ghani, Naveed Ahmed Khan, Ruqaiyyah Siddiqui, Shafiq Aazmi, Hasseri Halim, Tengku Shahrul Anuar Pathogens.2022; 11(12): 1474. CrossRef Differential expression of Acanthamoeba castellanii proteins during amoebic keratitis in rats Ana Carolina Carvalho-Silva, Camila H. Coelho, Cecília Cirelli, Frederico Crepaldi, Isabela Aurora Rodrigues-Chagas, Cinthia Furst, Daniel Carvalho Pimenta, Juliano Simões de Toledo, Ana Paula Fernandes, Adriana Oliveira Costa Experimental Parasitology.2021; 221: 108060. CrossRef Peganum harmala Extract Has Antiamoebic Activity to Acanthamoeba triangularis Trophozoites and Changes Expression of Autophagy-Related Genes Rachasak Boonhok, Suthinee Sangkanu, Julalak Chuprom, Mayuna Srisuphanunt, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Chr Pathogens.2021; 10(7): 842. CrossRef Extracellular protease profile of Acanthamoeba after prolonged axenic culture and after interaction with MDCK cells Cecília Cirelli, Elaine Isabela Soares Mesquita, Isabela Aurora Rodrigues Chagas, Cinthia Furst, Cynara Oliveira Possamai, Jonatas Santos Abrahão, Ludmila Karen dos Santos Silva, Marina Felipe Grossi, Carlos Alberto Tagliati, Adriana Oliveira Costa Parasitology Research.2020; 119(2): 659. CrossRef Busting biofilms: free-living amoebae disrupt preformed methicillin-resistant Staphylococcus aureus (MRSA) and Mycobacterium bovis biofilms Kevin H. Martin, Grace I. Borlee, William H. Wheat, Mary Jackson, Bradley R. Borlee Microbiology .2020; 166(8): 695. CrossRef Anti-amoebic potential of azole scaffolds and nanoparticles against pathogenic Acanthamoeba Shweta Walvekar, Ayaz Anwar, Areeba Anwar, Nanthini Sridewi, Mohammad Khalid, Yoon Yen Yow, Naveed Ahmed Khan Acta Tropica.2020; 211: 105618. CrossRef Identification and biochemical characterisation of Acanthamoeba castellanii cysteine protease 3 Zhixin Wang, Duo Wu, Hiroshi Tachibana, Meng Feng, Xun-jia Cheng Parasites & Vectors.2020;[Epub] CrossRef Extracellular vesicles and vesicle-free secretome of the protozoa Acanthamoeba castellanii under homeostasis and nutritional stress and their damaging potential to host cells Diego de Souza Gonçalves, Marina da Silva Ferreira, Susie Coutinho Liedke, Kamilla Xavier Gomes, Gabriel Afonso de Oliveira, Pedro Ernesto Lopes Leão, Gabriele Vargas Cesar, Sergio H. Seabra, Juliana Reis Cortines, Arturo Casadevall, Leonardo Nimrichter, Virulence.2018; 9(1): 818. CrossRef Characterization of extracellular proteases of Acanthamoeba genotype T4 isolated from different sources in Iran Behroz Mahdavi Poor, Abdolhossein Dalimi, Fatemeh Ghafarifar, Fariba Khoshzaban, Jalal Abdolalizadeh Parasitology Research.2017; 116(12): 3373. CrossRef Characterisation and expression analysis of trophozoite and cyst proteins of Acanthamoeba spp. isolated from Acanthamoeba keratitis (AK) patient Himansu Sekhar Behera, Gita Satpathy Molecular and Biochemical Parasitology.2016; 205(1-2): 29. CrossRef Microbial collagenases: challenges and prospects in production and potential applications in food and nutrition Gaurav Kumar Pal, Suresh PV RSC Advances.2016; 6(40): 33763. CrossRef Acanthamoeba culbertsoni: Electron‐Dense Granules in a Highly Virulent Clinical Isolate Bibiana Chávez‐Munguía, Lizbeth Salazar‐Villatoro, Maritza Omaña‐Molina, Martha Espinosa‐Cantellano, Elizabeth Ramírez‐Flores, Jacob Lorenzo‐Morales, Adolfo Martínez‐Palomo Journal of Eukaryotic Microbiology.2016; 63(6): 744. CrossRef Diversity, Structures, and Collagen-Degrading Mechanisms of Bacterial Collagenolytic Proteases Yu-Zhong Zhang, Li-Yuan Ran, Chun-Yang Li, Xiu-Lan Chen, F. E. Löffler Applied and Environmental Microbiology.2015; 81(18): 6098. CrossRef Acanthamoeba Protease Activity Promotes Allergic Airway Inflammation via Protease-Activated Receptor 2 Mi Kyung Park, Min Kyoung Cho, Shin Ae Kang, Hye-Kyung Park, Dong-Hee Kim, Hak Sun Yu, Venuprasad K. Poojary PLoS ONE.2014; 9(3): e92726. CrossRef Characterization of a Novel Subtilisin-like Protease Myroicolsin from Deep Sea Bacterium Myroides profundi D25 and Molecular Insight into Its Collagenolytic Mechanism Li-Yuan Ran, Hai-Nan Su, Ming-Yang Zhou, Lei Wang, Xiu-Lan Chen, Bin-Bin Xie, Xiao-Yan Song, Mei Shi, Qi-Long Qin, Xiuhua Pang, Bai-Cheng Zhou, Yu-Zhong Zhang, Xi-Ying Zhang Journal of Biological Chemistry.2014; 289(9): 6041. CrossRef Structural and mechanistic insights into collagen degradation by a bacterial collagenolytic serine protease in the subtilisin family Li‐Yuan Ran, Hai‐Nan Su, Guo‐Yan Zhao, Xiang Gao, Ming‐Yang Zhou, Peng Wang, Hui‐Lin Zhao, Bin‐Bin Xie, Xi‐Ying Zhang, Xiu‐Lan Chen, Bai‐Cheng Zhou, Yu‐Zhong Zhang Molecular Microbiology.2013; 90(5): 997. CrossRef Pathological characteristics of the different stages of Acanthamoeba keratitis Yuzhao Sun, Jing Hong, Pei Zhang, Rongmei Peng, Gege Xiao Histopathology.2013; 63(6): 862. CrossRef Proteases fromEntamoebaspp. and Pathogenic Free-Living Amoebae as Virulence Factors Jesús Serrano-Luna, Carolina Piña-Vázquez, Magda Reyes-López, Guillermo Ortiz-Estrada, Mireya de la Garza Journal of Tropical Medicine.2013; 2013: 1. CrossRef Acanthamoeba interactions with the blood–brain barrier under dynamic fluid flow James Edwards-Smallbone, Richard J. Pleass, Naveed A. Khan, Robin J. Flynn Experimental Parasitology.2012; 132(3): 367. CrossRef Host-Parasite Interaction: Parasite-Derived and -Induced Proteases That Degrade Human Extracellular Matrix Carolina Piña-Vázquez, Magda Reyes-López, Guillermo Ortíz-Estrada, Mireya de la Garza, Jesús Serrano-Luna Journal of Parasitology Research.2012; 2012: 1. CrossRef Serine protease activities in Leishmania (Leishmania) chagasi promastigotes Raquel Elisa da Silva-López, Tatiana Resende dos Santos, José Andrés Morgado-Díaz, Marcelo Neves Tanaka, Salvatore Giovanni de Simone Parasitology Research.2010; 107(5): 1151. CrossRef Pathogenesis of Acanthamoeba Keratitis Noorjahan Panjwani The Ocular Surface.2010; 8(2): 70. CrossRef Acanthamoeba culbertsoni Elicits Soluble Factors That Exert Anti-Microglial Cell Activity Jenica L. Harrison, Gabriela A. Ferreira, Erinn S. Raborn, Audrey D. Lafrenaye, Francine Marciano-Cabral, Guy A. Cabral Infection and Immunity.2010; 78(9): 4001. CrossRef Differential effects of α-helical and β-hairpin antimicrobial peptides against Acanthamoeba castellanii R. S. SACRAMENTO, R. M. MARTINS, A. MIRANDA, A. S. S. DOBROFF, S. DAFFRE, A. S. FORONDA, D. DE FREITAS, S. SCHENKMAN Parasitology.2009; 136(8): 813. CrossRef Elastase secretion in Acanthamoeba polyphaga Gabriela A. Ferreira, Ana C.M. Magliano, Elizabeth M.F. Pral, Silvia C. Alfieri Acta Tropica.2009; 112(2): 156. CrossRef Characterization of a Serine Proteinase Mediating Encystation of Acanthamoeba Eun-Kyung Moon, Dong-Il Chung, Yeon-Chul Hong, Hyun-Hee Kong Eukaryotic Cell.2008; 7(9): 1513. CrossRef Differentially expressed genes of Acanthamoeba castellanii during encystation Eun-Kyung Moon, Dong-Il Chung, Yeon-Chul Hong, Hyun-Hee Kong The Korean Journal of Parasitology.2007; 45(4): 283. CrossRef 11,248 View 88 Download Crossref Molecular and biochemical characterization of hemoglobinase, a cysteine proteinase, in Paragonimus westermani Joon-Hyuck Choi, Jae-Hyuk Lee, Hak-Sun Yu, Hae-Jin Jeong, Jin Kim, Yeon-Chul Hong, Hyun-Hee Kong, Dong-Il Chung Korean J Parasitol 2006;44(3):187-196. Published online September 20, 2006 DOI: https://doi.org/10.3347/kjp.2006.44.3.187 Abstract PDF The mammalian trematode Paragonimus westermani is a typical digenetic parasite, which can cause paragonimiasis in humans. Host tissues and blood cells are important sources of nutrients for development, growth and reproduction of P. westermani. In this study, a cDNA clone encoding a 47 kDa hemoglobinase of P. westermani was characterized by sequencing analysis, and its localization was investigated immunohistochemically. The phylogenetic tree prepared based on the hemoglobinase gene showed high homology with hemoglobinases of Fasciola hepatica and Schistosoma spp. Moreover, recombinant P. westermani hemoglobinase degradaded human hemoglobin at acidic pH (from 3.0 to 5.5) and its activity was almost completely inhibited by E-64, a cysteine proteinase inhibitor. Immunohistochemical studies showed that P. westermani hemoglobinase was localized in the epithelium of the adult worm intestine implying that the protein has a specific function. These observations suggest that hemoglobinase may act as a digestive enzyme for acquisition of nutrients from host hemoglobin. Further investigations may provide insights into hemoglobin catabolism in P. westermani. Citations Citations to this article as recorded by Multi-stage transcriptome profiling of the neglected food-borne echinostome Artyfechinostomum sufrartyfex reveal potential diagnostic and drug targets Suman Dahal, Pratibha Gour, Saurabh Raghuvanshi, Yugal Kishore Prasad, Dipshikha Saikia, Sudeep Ghatani Acta Tropica.2022; 233: 106564. CrossRef Characterization and localization of antigens for serodiagnosis of human paragonimiasis Kurt C. Curtis, Kerstin Fischer, Young-Jun Choi, Makedonka Mitreva, Gary J. Weil, Peter U. Fischer Parasitology Research.2021; 120(2): 535. CrossRef Cysteine proteases as digestive enzymes in parasitic helminths Conor R. Caffrey, Louise Goupil, Karina M. Rebello, John P. Dalton, David Smith, Aaron R. Jex PLOS Neglected Tropical Diseases.2018; 12(8): e0005840. CrossRef Adult Opisthorchis felineus major protein fractions deduced from transcripts: Comparison with liver flukes Opisthorchis viverrini and Clonorchis sinensis Mikhail Pomaznoy, Sergey Tatkov, Alexey Katokhin, Dmitry Afonnikov, Vladimir Babenko, Dagmara Furman, Ilya Brusentsov, Pavel Belavin, Alexandr Najakshin, Sergey Guselnikov, Gennady Vasiliev, Anton Sivkov, Egor Prokhortchouk, Konstantin Skryabin, Viatchesl Experimental Parasitology.2013; 135(2): 297. CrossRef Paragonimus worm from a New Guinea native in 1926 Wenlin Wang, David Blair, Tian Min, Fang Li, Dianhua Wang Asian Pacific Journal of Tropical Medicine.2011; 4(1): 76. CrossRef Asparaginyl endopeptidase from the carcinogenic liver fluke, Opisthorchis viverrini, and its potential for serodiagnosis Thewarach Laha, Jittiyawadee Sripa, Banchob Sripa, Mark Pearson, Leon Tribolet, Sasithorn Kaewkes, Paiboon Sithithaworn, Paul J. Brindley, Alex Loukas International Journal of Infectious Diseases.2008; 12(6): e49. CrossRef 9,947 View 79 Download Crossref Evaluation of taxonomic validity of four species of Acanthamoeba: A. divionensis, A. paradivionensis, A. mauritaniensis, and A. rhysodes, inferred from molecular analyses Hua Liu, Eun-Kyung Moon, Hak-Sun Yu, Hae-Jin Jeong, Yeon-Chul Hong, Hyun-Hee Kong, Dong-Il Chung Korean J Parasitol 2005;43(1):7-13. Published online March 20, 2005 DOI: https://doi.org/10.3347/kjp.2005.43.1.7 Abstract PDF The taxonomy of Acanthamoeba spp., an amphizoic amoeba which causes granulomatous amoebic encephalitis and chronic amoebic keratitis, has been revised many times. The taxonomic validity of some species has yet to be assessed. In this paper, we analyzed the morphological characteristics, nuclear 18s rDNA and mitochondrial 16s rDNA sequences and the Mt DNA RFLP of the type strains of four Acanthamoeba species, which had been previously designated as A. divionensis, A. parasidionensis, A. mauritaniensis, and A. rhysodes. The four isolates revealed characteristic group II morphology. They exhibited 18S rDNA sequence differences of 0.2-1.1% with each other, but more than 2% difference from the other compared reference strains. Four isolates formed a different clade from that of A. castellanii Castellani and the other strains in morphological group II on the phylogenetic tree. In light of these results, A. paradivionensis, A. divionensis, and A. mauritaniensis should be regarded as synonyms for A. rhysodes. Citations Citations to this article as recorded by The Status of Molecular Analyses of Isolates of Acanthamoeba Maintained by International Culture Collections Paul A. Fuerst Microorganisms.2023; 11(2): 295. CrossRef On the diversity and clinical importance of Acanthamoeba spp. from Group 1 Daniele Corsaro Parasitology Research.2021; 120(6): 2057. CrossRef Isolates from ancient permafrost help to elucidate species boundaries in Acanthamoeba castellanii complex (Amoebozoa: Discosea) Stas Malavin, Lyubov Shmakova European Journal of Protistology.2020; 73: 125671. CrossRef Update on Acanthamoeba phylogeny Daniele Corsaro Parasitology Research.2020; 119(10): 3327. CrossRef Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA Md Moshiur Rahman, Kenji Yagita, Akira Kobayashi, Yosaburo Oikawa, Amjad I.A. Hussein, Takahiro Matsumura, Masaharu Tokoro The Korean Journal of Parasitology.2013; 51(4): 401. CrossRef Isolation and characterization of Acanthamoeba spp. from air-conditioners in Kuala Lumpur, Malaysia Li-Li Chan, Joon-Wah Mak, Yoon-Tong Low, Thuan-Tzen Koh, Init Ithoi, Shar Mariam Mohamed Acta Tropica.2011; 117(1): 23. CrossRef Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung The Korean Journal of Parasitology.2008; 46(3): 157. CrossRef Acanthamoeba keratitis due to Acanthamoeba genotype T4 in a non-contact-lens wearer in Turkey Hatice Ertabaklar, Meral Türk, Volkan Dayanir, Sema Ertuğ, Julia Walochnik Parasitology Research.2007; 100(2): 241. CrossRef 8,958 View 78 Download Crossref Isolation and characterization of a cDNA encoding a mammalian cathepsin L-like cysteine proteinase from Acanthamoeba healyi Yeon-Chul Hong, Mi-Yul Hwang, Ho-Cheol Yun, Hak-Sun Yu, Hyun-Hee Kong, Tai-Soon Yong, Dong-Il Chung Korean J Parasitol 2002;40(1):17-24. Published online March 31, 2002 DOI: https://doi.org/10.3347/kjp.2002.40.1.17 Abstract PDF We have cloned a cDNA encoding a cysteine proteinase of the Acanthamoeba healyi OC-3A strain isolated from the brain of a granulomatous amoebic encephalitis patient. A DNA probe for an A. healyi cDNA library screening was amplified by PCR using degenerate oligonucleotide primers designed on the basis of conserved amino acids franking the active sites of cysteine and asparagine residues that are conserved in the eukaryotic cysteine proteinases. Cysteine proteinase gene of A. healyi (AhCP1) was composed of 330 amino acids with signal sequence, a proposed pro-domain and a predicted active site made up of the catalytic residues, Cys²⁵, His¹⁵⁹, and Asn¹⁷⁵. Deduced amino acid sequence analysis indicates that AhCP1 belong to ERFNIN subfamily of C1 peptidases. By Northern blot analysis, no direct correlation was observed between AhCP1 mRNA expression and virulence of Acanthamoeba, but the gene was expressed at higher level in amoebae isolated from soil than amoeba from clinical samples. These findings raise the possibility that Ahcp1 protein may play a role in protein metabolism and digestion of phagocytosed bacteria or host tissue debris rather than in invasion of amoebae into host tissue. Citations Citations to this article as recorded by Amoebic encephalitis within Australia Samantha C. Carija, Avram Levy, Graham Weaire‐Buchanan, Terence Lee, Robin Woodward, Jake Gazeley, Troy A. Edwards, Jason M. Dyke, Vicki Fabian, Katherine Norton, Andrew Chapman, Paul R. Ingram Internal Medicine Journal.2025; 55(1): 143. CrossRef Biological characteristics and pathogenicity of Acanthamoeba Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li Frontiers in Microbiology.2023;[Epub] CrossRef Identification and biochemical characterisation of Acanthamoeba castellanii cysteine protease 3 Zhixin Wang, Duo Wu, Hiroshi Tachibana, Meng Feng, Xun-jia Cheng Parasites & Vectors.2020;[Epub] CrossRef Anti-amoebic potential of azole scaffolds and nanoparticles against pathogenic Acanthamoeba Shweta Walvekar, Ayaz Anwar, Areeba Anwar, Nanthini Sridewi, Mohammad Khalid, Yoon Yen Yow, Naveed Ahmed Khan Acta Tropica.2020; 211: 105618. CrossRef Host Invasion by Pathogenic Amoebae: Epithelial Disruption by Parasite Proteins Abigail Betanzos, Cecilia Bañuelos, Esther Orozco Genes.2019; 10(8): 618. CrossRef Molecular and Biochemical Properties of a Cysteine Protease of Acanthamoeba castellanii Yeonchul Hong, Jung-Mi Kang, So-Young Joo, Su-Min Song, Hương Giang Lê, Thị Lam Thái, Jinyoung Lee, Youn-Kyoung Goo, Dong-Il Chung, Woon-Mok Sohn, Byoung-Kuk Na The Korean Journal of Parasitology.2018; 56(5): 409. CrossRef Cysteine protease involving in autophagosomal degradation of mitochondria during encystation of Acanthamoeba Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Hyun-Hee Kong Molecular and Biochemical Parasitology.2012; 185(2): 121. CrossRef Acanthamoeba culbertsoni Elicits Soluble Factors That Exert Anti-Microglial Cell Activity Jenica L. Harrison, Gabriela A. Ferreira, Erinn S. Raborn, Audrey D. Lafrenaye, Francine Marciano-Cabral, Guy A. Cabral Infection and Immunity.2010; 78(9): 4001. CrossRef Characterization of a Serine Proteinase Mediating Encystation of Acanthamoeba Eun-Kyung Moon, Dong-Il Chung, Yeon-Chul Hong, Hyun-Hee Kong Eukaryotic Cell.2008; 7(9): 1513. CrossRef Differentially expressed genes of Acanthamoeba castellanii during encystation Eun-Kyung Moon, Dong-Il Chung, Yeon-Chul Hong, Hyun-Hee Kong The Korean Journal of Parasitology.2007; 45(4): 283. CrossRef Acanthamoeba: biology and increasing importance in human health Naveed Ahmed Khan FEMS Microbiology Reviews.2006; 30(4): 564. CrossRef Intracellular Localization and Trafficking of Serine Proteinase AhSub and Cysteine Proteinase AhCP of Acanthamoeba healyi E.-K. Moon, S.-T. Lee, D.-I. Chung, H.-H. Kong Eukaryotic Cell.2006; 5(1): 125. CrossRef Pathogenic free-living amoebae in Korea Ho-Joon Shin, Kyung-il Im The Korean Journal of Parasitology.2004; 42(3): 93. CrossRef 8,305 View 96 Download Crossref