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Yong-Joo Kim	2 Articles
Development and Clinical Evaluation of a Rapid Serodiagnostic Test for Toxoplasmosis of Cats Using Recombinant SAG1 Antigen Chom-Kyu Chong, Wooseog Jeong, Hak-Yong Kim, Dong-Jun An, Hye-Young Jeoung, Jeong-Eun Ryu, A-Ra Ko, Yong-Joo Kim, Sung-Jong Hong, Zhaoshou Yang, Ho-Woo Nam Korean J Parasitol 2011;49(3):207-212. Published online September 30, 2011 DOI: https://doi.org/10.3347/kjp.2011.49.3.207 Abstract PDF Rapid serodiagnostic methods for Toxoplasma gondii infection in cats are urgently needed for effective control of transmission routes toward human infections. In this work, 4 recombinant T. gondii antigens (SAG1, SAG2, GRA3, and GRA6) were produced and tested for the development of rapid diagnostic test (RDT). The proteins were expressed in Escherichia coli, affinity-purified, and applied onto the nitrocellulose membrane of the test strip. The recombinant SAG1 (rSAG1) showed the strongest antigenic activity and highest specificity among them. We also performed clinical evaluation of the rSAG1-loaded RDT in 182 cat sera (55 household and 127 stray cats). The kit showed 0.88 of kappa value comparing with a commercialized ELISA kit, which indicated a significant correlation between rSAG1-loaded RDT and the ELISA kit. The overall sensitivity and specificity of the RDT were 100% (23/23) and 99.4% (158/159), respectively. The rSAG1-loaded RDT is rapid, easy to use, and highly accurate. Thus, it would be a suitable diagnostic tool for rapid detection of antibodies in T. gondii-infected cats under field conditions. Citations Citations to this article as recorded by Development and optimization of an ELISA method to detect Toxoplasma gondii oocyst infection in cats Mingfeng He, Bufan Zhang, Shuai Han, Jiahui Qian, Zhengming He, Yulian Wei, Yanqin Zhou, Bang Shen, Rui Fang Parasitology Research.2025;[Epub] CrossRef Comprehensive diagnostic approaches to feline toxoplasmosis: Bridging traditional methods and emerging technologies Dan Zhao, Yanzhen Liao, Hao Liu, Jianwei Wang, Ruiying Liang, Rongqiong Zhou, Jiabo Ding, Sixin Zhang, Xinming Tang Virulence.2025;[Epub] CrossRef Evaluation of an immunochromatographic serologic test to detect the presence of anti‐Toxoplasma gondii antibodies in cats Sergio Villanueva‐Saz, Mariví Martínez, Jacobo Giner, María Dolores Pérez, Ana Pilar Tobajas, Andrés Yzuel, María Teresa Verde, Delia Lacasta, Antonio Fernández, Diana Marteles, Héctor Ruíz Veterinary Clinical Pathology.2023; 52(2): 284. CrossRef A preliminary study to develop a lateral flow assay using recombinant GRA1 protein for the diagnosis of toxoplasmosis in stray cats Aysu Değirmenci Döşkaya, Hüseyin Can, Aytül Gül, Tuğba Karakavuk, Mervenur Güvendi, Muhammet Karakavuk, Ceren Gül, Sedef Erkunt Alak, Cemal Ün, Adnan Yüksel Gürüz, Mert Döşkaya Comparative Immunology, Microbiology and Infectious Diseases.2023; 101: 102057. CrossRef Development and evaluation of indirect enzyme-linked immunosorbent assay using recombinant dense granule antigen 7 protein for the detection of Toxoplasma gondii infection in cats in Thailand Eukote Suwan, Piangjai Chalermwong, Rucksak Rucksaken, Metita Sussadee, Sarawan Kaewmongkol, Ruenruetai Udonsom, Sathaporn Jittapalapong, Bandid Mangkit Veterinary World.2022; : 602. CrossRef Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals Amjad Hayat Khan, Rahmah Noordin European Journal of Clinical Microbiology & Infectious Diseases.2020; 39(1): 19. CrossRef All about toxoplasmosis in cats: the last decade J.P. Dubey, C.K. Cerqueira-Cézar, F.H.A. Murata, O.C.H. Kwok, Y.R. Yang, C. Su Veterinary Parasitology.2020; 283: 109145. CrossRef Toxoplasma gondii Recombinant Antigens in the Serodiagnosis of Toxoplasmosis in Domestic and Farm Animals Bartłomiej Ferra, Lucyna Holec-Gąsior, Weronika Grąźlewska Animals.2020; 10(8): 1245. CrossRef Fluorescent bead-based serological detection of Toxoplasma gondii infection in chickens Benedikt T. Fabian, Fatima Hedar, Martin Koethe, Berit Bangoura, Pavlo Maksimov, Franz J. Conraths, Isabelle Villena, Dominique Aubert, Frank Seeber, Gereon Schares Parasites & Vectors.2020;[Epub] CrossRef Development and Clinical Evaluation of a Rapid Diagnostic Test for Yellow Fever Non-Structural Protein 1 Yeong Hoon Kim, Tae-Yun Kim, Ji-Seon Park, Jin Suk Park, Jihoo Lee, Joungdae Moon, Chom-Kyu Chong, Ivan Neves Junior, Fernando Raphael Ferry, Hye-Jin Ahn, Lokraj Bhatt, Ho-Woo Nam The Korean Journal of Parasitology.2019; 57(3): 283. CrossRef Development of a Rapid Diagnostic Test Kit to Detect IgG/IgM Antibody against Zika Virus Using Monoclonal Antibodies to the Envelope and Non-structural Protein 1 of the Virus Yeong Hoon Kim, Jihoo Lee, Young-Eun Kim, Chom-Kyu Chong, Yanaihara Pinchemel, Francis Reisdörfer, Joyce Brito Coelho, Ronaldo Ferreira Dias, Pan Kee Bae, Zuinara Pereira Maia Gusmão, Hye-Jin Ahn, Ho-Woo Nam The Korean Journal of Parasitology.2018; 56(1): 61. CrossRef Disentangling the link between supplemental feeding, population density, and the prevalence of pathogens in urban stray cats Jusun Hwang, Nicole L. Gottdenker, Dae-Hyun Oh, Ho-Woo Nam, Hang Lee, Myung-Sun Chun PeerJ.2018; 6: e4988. CrossRef Spread and genotype of Toxoplasma gondii in naturally infected alpine chamois (Rupicapra r. rupicapra) Nicoletta Formenti, Alessandra Gaffuri, Tiziana Trogu, Roberto Viganò, Nicola Ferrari, Paolo Lanfranchi Parasitology Research.2016; 115(5): 2115. CrossRef Opportunities and challenges for the application of microfluidic technologies in point-of-care veterinary diagnostics Valentina Busin, Beth Wells, Maïwenn Kersaudy-Kerhoas, Wenmaio Shu, Stewart T.G. Burgess Molecular and Cellular Probes.2016; 30(5): 331. CrossRef Synthesis and characterization of latex-protein complexes from different antigens ofToxoplasma gondiifor immunoagglutination assays Leandro E. Peretti, Verónica D. G. Gonzalez, Juan G. Costa, Iván S. Marcipar, Luis M. Gugliotta International Journal of Polymeric Materials and Polymeric Biomaterials.2016; 65(18): 938. CrossRef Toxoplasma gondii Infection in Alpine Red Deer (Cervus elaphus): Its Spread and Effects on Fertility Nicoletta Formenti, Tiziana Trogu, Luca Pedrotti, Alessandra Gaffuri, Paolo Lanfranchi, Nicola Ferrari, Guido Favia PLOS ONE.2015; 10(9): e0138472. CrossRef Latex–protein complexes from an acute phase recombinant antigen of Toxoplasma gondii for the diagnosis of recently acquired toxoplasmosis Leandro E. Peretti, Verónica D.G. Gonzalez, Iván S. Marcipar, Luis M. Gugliotta Colloids and Surfaces B: Biointerfaces.2014; 120: 88. CrossRef A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein Kyoung Ju Song, Zhaoshou Yang, Chom-Kyu Chong, Jin-Soo Kim, Kyung Chan Lee, Tong-Soo Kim, Ho-Woo Nam The Korean Journal of Parasitology.2013; 51(5): 503. CrossRef Evaluation of an indirect ELISA using a tachyzoite surface antigen SAG1 for diagnosis of Toxoplasma gondii infection in cats M. Hosseininejad Experimental Parasitology.2012; 132(4): 556. CrossRef 10,773 View 121 Download Crossref Maintained Seroprevalence of Toxoplasmosis among the Residents of Jeju Island, Korea Sung-Jong Hong, Chom-Kyu Chong, Kyutaeg Lee, Tong-Soo Kim, Yeon-Pyo Hong, Hye-Jin Ahn, Hak-Yong Kim, A-Ra Ko, Yong-Joo Kim, Ho-Woo Nam Korean J Parasitol 2011;49(3):309-311. Published online September 30, 2011 DOI: https://doi.org/10.3347/kjp.2011.49.3.309 Abstract PDF Seroepidemiological status of toxoplasmosis among the residents of Jeju island was surveyed and evaluated by ELISA with crude extract of Toxoplasma gondii. The sera of 2,348 residents (male 1,157 and female 1,191) were collected and checked for the IgG antibody titers, which showed 13.2% positive rate (309 sera). The positive rates were increasing gradually according to the age from 4.3% in teenage to 20.6% in seventies. The positive rates were significantly different between the sex by 16.2% for male and 10.2% for female (P<0.05). This positive rate of toxoplasmosis in Jeju island residents is regarded relatively higher than any other regions of Korea. And the high positive rate may be maintained continuously among Jeju island residents without any clear reasons until now but due to some parts peculiar socio-cultural tradition of Jeju island. Therefore, it is necessary to study further the epidemiology of toxoplasmosis of Jeju island. Citations Citations to this article as recorded by Seroprevalence of Toxoplasma gondii assayed using Rapid Diagnostic Tests among Residents in Three Counties Adjacent to The Demilitarized Zone, Korea Jeehi Jung, Jinyoung Lee, Yoon Kyung Chang, Seong Kyu Ahn, Seo Hye Park, Sung-Jong Hong, Jihoo Lee, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam, Tong-Soo Kim, Dongjae Kim The Korean Journal of Parasitology.2021; 59(1): 9. CrossRef Clusters of Toxoplasmosis in Ganghwa-gun, Cheorwon-gun, and Goseong-gun, Korea Jihye Yu, Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim The Korean Journal of Parasitology.2021; 59(3): 251. CrossRef Cross-sectional survey for Toxoplasma gondii infection in humans in Fernando de Noronha island, Brazil Maria da Conceição Carvalho, Müller Ribeiro-Andrade, Renata Pimentel Bandeira de Melo, Dandara Matias Guedes, José Wilton Pinheiro Junior, Erika Fernanda Torres Samico Fernandes Cavalcanti, Fernando Jorge Rodrigues Magalhães, Rinaldo Aparecido Mota Revista Brasileira de Parasitologia Veterinária.2021;[Epub] CrossRef Seroprevalence and B1 gene Phylogeny of Toxoplasma gondii of Dogs and Cats in Republic of Korea Yeojin Park, Jinhyeong Noh, Hyun-Ji Seo, Keun-Ho Kim, Subin Min, Mi-Sun Yoo, Bo-Ram Yun, Jong-Ho Kim, Eun-Jin Choi, Doo-Sung Cheon, Sung-Jong Hong, Soon-Seek Yoon, Yun Sang Cho The Korean Journal of Parasitology.2020; 58(3): 257. CrossRef Clusters of Toxoplasmosis in Gyodong-Myeon and Samsan-Myeon, Ganghwa-Gun, Korea Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim The Korean Journal of Parasitology.2020; 58(5): 493. CrossRef Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets Won Gi Yoo, Sun-Min Kim, Eun Jeong Won, Ji-Yun Lee, Fuhong Dai, Ho Choon Woo, Ho-Woo Nam, Tae Im Kim, Jeong-Hee Han, Dongmi Kwak, Yun Sang Cho, Seung-Won Kang, Tong-Soo Kim, Xing-Quan Zhu, Chunren Wang, Heejeong Youn, Sung-Jong Hong The Korean Journal of Parasitology.2018; 56(5): 437. CrossRef Seroprevalence of Toxoplasmosis with ELISA and Rapid Diagnostic Test among Residents in Gyodong-do, Inchon city, Korea: A Four-Year Follow-up Yeong Hoon Kim, Ji hoo Lee, Seong kyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam The Korean Journal of Parasitology.2017; 55(3): 247. CrossRef Seroprevalence of Toxoplasmosis Detected by RDT in Residents near the DMZ (demilitarized zone) of Cheorwon-gun, Gangwon-do, Korea Yeong Hoon Kim, Jihoo Lee, Young-Eun Kim, Seongkyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam The Korean Journal of Parasitology.2017; 55(4): 385. CrossRef Koki Yakushiji JOURNAL OF WATER AND ENVIRONMENTAL ISSUES.2015; 28(1): 82. CrossRef Mesial Temporal Lobe Epilepsy in Congenital Toxoplasmosis: A Case Report Woo Kyo Jeong, Byung-Euk Joo, Ji-hye Seo, Jun Kyu Mun, Juhyeon Kim, Dae-Won Seo Journal of Epilepsy Research.2015; 5(1): 25. CrossRef Toxoplasma gondii Infection: Seroprevalence and Associated Risk Factors among Primary School Children in the Capital Area of the Republic of the Marshall Islands Chung-Jung Fu, Ting-Wu Chuang, Huei-Shan Lin, Chih-Hsiung Wu, Yung-Ching Liu, Mailynn K. Langinlur, Min-Yun Lu, Wesley Wei-Wen Hsiao, Chia-Kwung Fan Japanese Journal of Infectious Diseases.2014; 67(5): 405. CrossRef A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein Kyoung Ju Song, Zhaoshou Yang, Chom-Kyu Chong, Jin-Soo Kim, Kyung Chan Lee, Tong-Soo Kim, Ho-Woo Nam The Korean Journal of Parasitology.2013; 51(5): 503. CrossRef Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea Hyemi Lim, Sang-Eun Lee, Bong-Kwang Jung, Min-Ki Kim, Mi Youn Lee, Ho-Woo Nam, Jong-Gyun Shin, Cheong-Ha Yun, Han-Ik Cho, Eun-Hee Shin, Jong-Yil Chai The Korean Journal of Parasitology.2012; 50(4): 287. CrossRef A Surge in the Seroprevalence of Toxoplasmosis among the Residents of Islands in Gangwha-gun, Incheon, Korea Zhaoshou Yang, Pyo Yun Cho, Seong Kyu Ahn, Hye-Jin Ahn, Tong-Soo Kim, Chom-Kyu Chong, Sung-Jong Hong, Seok Ho Cha, Ho-Woo Nam The Korean Journal of Parasitology.2012; 50(3): 191. CrossRef Seroprevalence of Toxoplasmosis in the Residents of Cheorwon-gun, Gangwon-do, Korea Hye-Jin Ahn, Pyo Yun Cho, Seong Kyu Ahn, Tong-Soo Kim, Chom-Kyu Chong, Sung-Jong Hong, Seok Ho Cha, Ho-Woo Nam The Korean Journal of Parasitology.2012; 50(3): 225. CrossRef Acute Toxoplasmosis Mimicking Melanoma Metastases: Review of Conditions Causing False-Positive Results on ¹⁸F-FDG PET/CT K. Ivanova, K. Glatz, A. Zippelius, G. Nicolas, P. Itin Dermatology.2012; 225(4): 349. CrossRef 8,742 View 82 Download Crossref