Antigenic components in the crude extracts of Spirometra mansoni plerocercoid were analyzed in early experimental infections and in IgG subclass observed in clinical sparganosis. By IgG immunoblot, sera obtained serially from experimental mice, fed 5 spargana each, were reacted with the crude extracts. Protein bands at 36-26 kDa and 103 kDa showed positive reactions since two weeks after infection. In a differential immunoblot, in which a monospecific antibody against sparganum chymase at 36 kDa was pre-treated, the reactions at 36-26 kDa disappeared, indicating that the sparganum chymase and its degradation products invoked IgG antibody reactions. When 69 patients sera of human sparganosis were examined for their IgG subclass responses, IgG4 levels showed the highest reaction which was followed by IgG1. The IgG4 antibody also reacted mainly with 36-31 kDa protease. These results indicate that 36 kDa chymase of S. mansoni plerocercoid is the main antigenic component inducing IgG antibody response in early stage of experimental sparganosis and for specific IgG subclass reactions in human sparganosis.
Citations
Citations to this article as recorded by
Sparganosis S. B. Chuelov, A. L. Rossina CHILDREN INFECTIONS.2024; 23(1): 44. CrossRef
Establishment of Animal Infection Model of Spirometra Mansoni and Identification of Spirometra Mansoni by Enzyme-Linked Immunosorbent Assay Anqi Luo, Shuyu Chen, Mingye He, Xiaoruo Tan, Zhikang Li, Wei Liu, Yisong Liu Vector-Borne and Zoonotic Diseases.2024;[Epub] CrossRef
Evaluation of serum protein electrophoresis and immunofixation in dogs seropositive for various vector‐borne pathogens Oriol Jornet‐Rius, Nida Chornarm, Niki Skeldon, Ashley McGrew, Michael Lappin, Laia Solano‐Gallego, A Russell Moore Veterinary Clinical Pathology.2024; 53(4): 420. CrossRef
Immunoglobulin G4‐related disease in a dog Lydia J. Colopy, Kai‐Biu Shiu, Laura A. Snyder, Anne C. Avery, Emily D. Rout, A R. Moore Journal of Veterinary Internal Medicine.2019; 33(6): 2732. CrossRef
A Case of Sparganosis by Ingestion of Undercooked Snakes That Mimicked Peritoneal Carcinomatosis Yun Ku Kim, Myung Hwan Kim, Han Seoung Park, Ji Woong Jang, Yil Sik Hyun, Ji Hun Kim Korean Journal of Medicine.2012; 82(3): 331. CrossRef
A Protein Microarray for the Rapid Screening of Patients Suspected of Infection with Various Food-Borne Helminthiases Jia-Xu Chen, Mu-Xin Chen, Lin Ai, Jun-Hu Chen, Shao-Hong Chen, Yong-Nian Zhang, Yu-Chun Cai, Xing-Quan Zhu, Xiao-Nong Zhou, Patrick J. Lammie PLoS Neglected Tropical Diseases.2012; 6(11): e1899. CrossRef
Two-dimensional immunoblot analysis of antigenic proteins of Spirometra plerocercoid recognized by human patient sera Moizur Rahman, Eung-Goo Lee, Young-An Bae Parasitology International.2011; 60(2): 139. CrossRef
Production of Polyclonal Antibodies against the Tegument of Sparganum (Plerocercoid of Spirometra mansoni) and Its Immunolocalization Hyun-Jong Yang The Korean Journal of Parasitology.2010; 48(2): 183. CrossRef
Diagnostic values of IgG4 in human gnathostomiasis Malinee T. Anantaphruti, S. Nuamtanong, P. Dekumyoy Tropical Medicine & International Health.2005; 10(10): 1013. CrossRef
