This study was performed to observe the chronological changes in the tegumental structure of M. yokogawai using scanning electron microscope. The subjected worms were excysted metacercariae obtained from sweetfish, and 2-day, 1-week and 4-week old worms experimentally reared in albino rats. The results are as follows: The tegument of excysted metacercariae showed many transverse shallow rugae, which were more remarkable in posterior half body, i.e., posterior to ventral sucker. The whole surface was armed with many scale-like spines; 7-8 pointed ones on anterior body and 2-3 pointed on posterior body. The ciliated knob-like papillae (Type I) were abundant around oral and ventral suckers, which grouped 2, 3 or 4 in number in most cases. A few round swellings of tegument (Type II) were observed only on oral sucker. The tegumental surface of 2-day old worms showed deeper rugae, and the anterior half covered with knob-like processes of distal cytoplasm and the posterior half with cobblestone-like ones. Interspinous space became more wide and 9 pointed spines appeared on anterior dorsal surface.
The sensory papillae enlarged but not changed in their distribution. The tegument of 1-week old worms revealed knob-like cytoplasmic processes in posterior half body and velvety ones around oral sucker. The scale-Iike spines of anterior half body changed remarkably to the slender ones of posterior body at the level of ventral sucker. In dorsal surface, the arrangement of the Type I papillae were bilaterally symmetrical. The tegument of 4-week old worms were finely differentiated and the posterior tegument covered with velvety cytoplasmic processes. The spines had remarkably grown in length and width but the density remained nearly unchanged. The papillae also became larger but their morphology and distribution were not different from younger worms. However, the round elevation of cytoplasmic ridges (Type III papilla) appeared bilaterally on inner wall of oral sucker, approximately 8 in number.
From the above results, it is considered that the tegument of juvenile M. yokogawai continued to differentiate until 4 weeks after infection.
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A number of studies on the papillae of cercariae of trematodes reported that the papillar patterns (or chaetotaxy) of cercariae might be an excellent method to attain better understanding of the digenetic trematodes (Richard, 1971; Short and Cartrett, 1973; Bayssade-Dufour, 1979). The present study was aimed to determine the number, distribution pattern and structure of the sensory papillae of Metagonimus yokogawai cercariae, and to elucidate the chaetotaxy of this digenetic trematode. M. yokogawai cercariae were pipetted from a vial in which infected snails (Semisulcospira libertica) had been kept for 3 hours. The snails were collected from an endemic area of M. yokogawai, Boseong river in west-southern part of Korea. Observations of papillae were based on light microscopy of those stained with silver nitrate, and on scanning electron microscopy.
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Intestinal trematodes of humans in Korea: Metagonimus, heterophyids and echinostomes J Y Chai, S H Lee The Korean Journal of Parasitology.1990; 28(Suppl): s103. CrossRef
Identification of Stictodora lari (Heterophyidae) metacercariae encysted in the brackish water fish, Acanthogobius flavimanus J Y Chai, S K Park, S J Hong, M H Choi, S H Lee The Korean Journal of Parasitology.1989; 27(4): 253. CrossRef
A human case of Stellantchasmus falcatus infection W M Sohn, J Y Chai, S H Lee The Korean Journal of Parasitology.1989; 27(4): 277. CrossRef
Identification of Stellantchamus falcatus Metacercariae encysted in mullets in Korea Jong Yil Chai, Woon Mok Sohn The Korean Journal of Parasitology.1988; 26(1): 65. CrossRef
Stictodora sp. (Trematoda: Heterophyidae) recovered from a man in Korea Jong Yil Chai, Sung Jong Hong, Soon Hyung Lee, Byong Seol Seo The Korean Journal of Parasitology.1988; 26(2): 127. CrossRef
Ten human cases of Fibricola seoulensis infection and mixed one with Stellantchasmus and Metagonimus Sung Tae Hong, Jong Yil Chai, Soon Hyung Lee The Korean Journal of Parasitology.1986; 24(1): 95. CrossRef
Heterophyid trematodes (Heterophyopsis continua, Pygidiopsis summa and Heterophyes heterophyes nocens) from domestic cats in Korea Kee Seon Eom, So Yeob Son, Joon Sang Lee, Han Jong Rim The Korean Journal of Parasitology.1985; 23(2): 197. CrossRef
Comparative morphology of eggs of heterophyids and Clonorchis sinensis causing human infections in Korea Soon Hyung Lee, Soon Wook Hwang, Jong Yil Chai, Byong Seol Seo The Korean Journal of Parasitology.1984; 22(2): 171. CrossRef
Studies on intestinal trematodes in Korea XIII. Two cases of natural human infection by Heterophyopsis continua and the status of metacercarial infection in brackish water fishes Byong Seol Seo, Soon Hyung Lee, Jong Yil Chai, Sung Jong Hong The Korean Journal of Parasitology.1984; 22(1): 51. CrossRef
Two cases of natural human infection by Heterophyopsis continua (Heterophyidae) were identified by collection of adult worms in 1983 in Korea. And in order to know the source of infection a study on fish intermediate hosts was performed along the southwestern coastal areas. The cases were 24 and 50-year old males residing in southern coastal areas. They had the clinical complaints of non-specific gastrointestinal symptoms such as epigastric pain and one of them also complained the discharge of tapeworm segments.
Praziquantel in single dose of 10~15 mg/kg was given followed by purgation with mangnesium salt and 2 and 46 specimens of H. continua were collected from the diarrheal stools. They said to have eaten raw flesh of several kinds of brackish water fishes. The results of fish examination for metacercarial infection were as follows: Seventeen (63.0%) out of 27 perches (Lateolabrax japonicus) and 10 (29.4%) out of 34 gobies (Acanthogobius flavimanus) harboured the metacercariae but none of 42 mullets (Mugil cephalus) were found to harbour them. The average metacercarial density in perches was 55.4 and 18.7 per fish respectively. And the majority of the metacercariae were collected from the muscle of body portions in these fishes.
After the metacercariae were experimentally fed to two rats and one young dog, two adult worms were recovered from the dog while none from the rats. These worms were also indentified as H. continua. From the results it was concluded that the brackish water fishes which the human cases said to have eaten were the source of infection.
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A new endemic focus of Heterophyes nocens, Pygidiopsis summa, and other intestinal flukes in a coastal area of Muan-gun, Chollanam-do J Y Chai, I M Kim, M Seo, S M Guk, J L Kim, W M Sohn, S H Lee The Korean Journal of Parasitology.1997; 35(4): 233. CrossRef
One human case of natural infection by Heterophyopsis continua and three other species of intestinal trematodes S J Hong, C K Chung, D H Lee, H C Woo The Korean Journal of Parasitology.1996; 34(1): 87. CrossRef
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Conger myriaster, a new second intermediate host of Heterophyopsis continua (Digenea: Heterophyidae) Ki Hong Kim, Eun Seok Choi, Han Jong Rim The Korean Journal of Parasitology.1996; 34(4): 282. CrossRef
Infection status with the metacercariae of heterophyid flukes in the brackish water fish from Haenam-gun, Chollanam-do, Korea W M Sohn, G G Han, W G Kho, J Y Chai, S H Lee The Korean Journal of Parasitology.1994; 32(3): 163. CrossRef
Stictodora fuscatum (Heterophyidae) metacercariae encysted in gobies, Acanthogobius flavimanus W M Sohn, J Y Chai, S H Lee The Korean Journal of Parasitology.1994; 32(3): 143. CrossRef
A numerical taxonomic study on heterophyid trematodes K H Kim, Y M Yoon, J S Lee, H J Rim The Korean Journal of Parasitology.1991; 29(1): 55. CrossRef
Intestinal trematodes of humans in Korea: Metagonimus, heterophyids and echinostomes J Y Chai, S H Lee The Korean Journal of Parasitology.1990; 28(Suppl): s103. CrossRef
Recovery rate, growth and development of Heterophyopsis continua in experimental chicks S J Hong, S H Lee, J Y Chai, B S Seo The Korean Journal of Parasitology.1990; 28(1): 53. CrossRef
Identification of Stictodora lari (Heterophyidae) metacercariae encysted in the brackish water fish, Acanthogobius flavimanus J Y Chai, S K Park, S J Hong, M H Choi, S H Lee The Korean Journal of Parasitology.1989; 27(4): 253. CrossRef
Stictodora sp. (Trematoda: Heterophyidae) recovered from a man in Korea Jong Yil Chai, Sung Jong Hong, Soon Hyung Lee, Byong Seol Seo The Korean Journal of Parasitology.1988; 26(2): 127. CrossRef
Plecoglossus altivelis as a new fish intermediate host of Heterophyopsis continua Seung Yull Cho, Suk Il Kim The Korean Journal of Parasitology.1985; 23(1): 173. CrossRef
Heterophyid trematodes (Heterophyopsis continua, Pygidiopsis summa and Heterophyes heterophyes nocens) from domestic cats in Korea Kee Seon Eom, So Yeob Son, Joon Sang Lee, Han Jong Rim The Korean Journal of Parasitology.1985; 23(2): 197. CrossRef
Transmission experiments on the host specificity ofHeterophyes species in 16 potential definitive hosts H. Taraschewski Zeitschrift f�r Parasitenkunde Parasitology Research.1985; 71(4): 505. CrossRef
Comparative morphology of eggs of heterophyids and Clonorchis sinensis causing human infections in Korea Soon Hyung Lee, Soon Wook Hwang, Jong Yil Chai, Byong Seol Seo The Korean Journal of Parasitology.1984; 22(2): 171. CrossRef
A mass fecal examination was performed in Seoul, Korea from December 1983 to March 1984 and 15 egg positive cases of Fibricola seoulensis were discovered. They were treated with praziquantel(Distocide) 20 mg/kg single dose and magnesium salt for purgation. The adult worms of F. seoulensis were collected from 6 of them. The number of the flukes ranged 1-78 by the case. The cases had no clinical mainfestation at all. They were all Korean males in twenties, and had the histories of eating the roast or raw snakes or frogs.
Fibricoliasis is expected to be prevalent among the snake-eaters in Korea. Further cases will be detected by sedulous differentiation of thin-shelled immature operculated eggs of 0.081-0.102mmn length. Praziquantel seems to be an excellent chemotherapeutic for fibricoliasis.
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Comparative growth and development of the metacercariae of Fibricola seoulensis (Trematoda: Diplostomidae) in vitro, in vivo and on the chick chorioallantois B S Seo The Korean Journal of Parasitology.1989; 27(4): 231. CrossRef
Clinical and histopathological findings in mice heavily infected with Fibricola seoulensis Sun Huh, Jong Yil Chai, Sung Tae Hong, Soon Hyung Lee The Korean Journal of Parasitology.1988; 26(1): 45. CrossRef
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Ten human cases of Fibricola seoulensis infection and mixed one with Stellantchasmus and Metagonimus Sung Tae Hong, Jong Yil Chai, Soon Hyung Lee The Korean Journal of Parasitology.1986; 24(1): 95. CrossRef
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Two cases of human infection by adult Spirometra erinacei were proved by collection of worms in Korea. The patients were 24 and 21-year old males residing in a mountainous village in Kangwon-do. They had the clinical complaints of abdominal pain and epigastric discomfort, revealing the diphyllobothriid eggs, 53~59 x 37~42 micrometer in size, in their stools. They were treated with 500 mg atabrine and purgated with magnesium salts, and 3~4 hours later several chains of tapeworm segments were recovered from the diarrheal stools. The recovered worms revealed the morphological characteristics of spiral-form or coiled uteri, separated vaginal opening from the cirrus sac, incorporated seminal vesicle into the cirrus sac, distribution of testes at the junction between proglottids, asymmetrical eggs with one pointed eggs, etc. and were identified as Spirometra erinacei(Rudolphi, 1819). The cases had the history of eating raw flesh of the snakes and these are considered the source of infection.
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She was successfully treated with quinine sulfate (25 mg/kg/day in three doses for 5 days) and trimethoprim/sulfamethoxazole (8 mg/kg/day in two doses for 5 days) orally, and repeated blood smear had been negative for malaria. This report also signifies the frst description of congenital malaria in Korea imported from Uppervolta in Africa. A brief review of related literature was made.
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In order to determine the infestation patterns for metacercaria of digenetic trematodes, several kinds of fresh-water and brackish-water fish were caught in River Hyungsan, Kyungpook Province, Korea and examined from March to October in 1983. They were collected by netting, fishig with rod and line, or using the bait of crushed oil cake in transparent plastic bowls, and were dissected into the flesh, scales and fins with tail, and then examined for the presence and infestation density of larval trematodes. Of the 11 kinds of fish examined, the encysted larvae of Exorchis oviformis were found all of them, Metagonimus yokogawai from 9 kinds, Cyathocotyle orientalis, Metacercaria hasegawai and Metorchis orientalis from 6 kinds, and Clonorchis sinensis from 4 kinds in the present study. In the intensity of infestation with Clonorchis sinensis, the Korean shiner, Gnathopogon atromaculatus Nichols et Pope, was the most heavily infected species, the average number of metacercariae per gram of fish flesh being 45.5, followed by the striped shiner, Puntungia herzi Herzenstein 11.6, and Southern top-mouthed minnow, Pseudorasbora parva (Temminck et Shlegel), 9.7. The intensity of infestation with the Metagonimus larvae in 8 species of fresh-water fish was relatively low, and the average number per gram of flesh varied from 0.23 to 9.1. In comparison, the brackish-water fish were more heavily infected and the mean number of cysts per gram of flesh in the sweetfish, Plecoglossus altivelis, was 32.9. The results of this study generally indicate that Clonorchis sinensis and Metagonimus yokogawai infection may occur by consuming raw fresh-water fish caught in the river Hyungsan, Kyungpook Province, Korea.
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Epidemiological survey on Metagonimus yokogawai infection in the eastern coast area of Kangwon-province, Korea Yung Kyum Ahn, Pyung Rim Chung, Keun Tae Lee, Chin Thack Soh The Korean Journal of Parasitology.1987; 25(1): 59. CrossRef
Metacercariae of Echinochasmus japonicus encysted in a fresh water fish, Pseudorasbora parva, and their development in experimental mice Jong Yil Chai, Song Jong Hong, Dong Woo Son, Soon Hyung Lee, Byong Seol Seo The Korean Journal of Parasitology.1985; 23(2): 221. CrossRef
Studies on intestinal trematodes in Korea XX. Four cases of natural human infection by Echinochasmus japonicus Byong Seol Seo, Soon Hyung Lee, Jong Yil Chai, Sung Jong Hong The Korean Journal of Parasitology.1985; 23(2): 214. CrossRef
Epidemiological studies on Metagonimus yokogawai infection in Samcheok-gun, Kangwon-do, Korea Yung Kyum Ahn The Korean Journal of Parasitology.1984; 22(2): 161. CrossRef
This study was performed to investigate the intestinal parasites (especially cestoda) in the guts of Theragra chalcogramma in Korea. The cestode larvae collected were morphologically observed and measured, and at the same time, microscopical study was done with the section slides of the cestode larvae. They were identified on the basis of the descriptions by Schmidt and Yamaguti. The results obtained in this study are as follows: The cestode larvae were identified as Khawia sinesis, Eulacistorhynchus chiloscyllius, Tentacularia coryphaenea, Pseudonybelinia odontacantha and Nybelinia lingualis. These are new species reported in Korea. The characteristics of Khawia sinesis are its scolex lacking loculi and broad, flat and fimbriate shape. Its neck is not separated from the body but a little constricted. Eulacistorhynchus chiloscyllius is characterized by fairly long and acraspedote scolex. The bulbs are long; retractor muscles are attached to bases of the bulbs. The two bothridia are oval or round in shape and lacking posterior notch. The tentacles are long and poeciloacanthus; double chainette present. Tentacularia coryphaenea is characterized by its long craspedote and subcylindrical scolex. The bothridia are separated, without free borders and spinous. The tentacles are short, slender, armed with solid hooks in spirals, similar except the base of tentacle. The tentacle sheaths are not twisted. The bulbs are ellipsoidal. Pseudonybelinia odontacantha is characterized by its craspedote scolex. The tentacles are inserted near anterior margin of bothridia and armed with hooks in spirals ascending left to right. The hooks are similar, throughout tentacle, with feeble curve and provided with a tooth-like protuberance on the ventral side. The bulbs are three times longer than its width. The posterior margins of bothridia have a pair of eversible ciliated pits; or fossettes. Nybelinia lingualis is characterized by its short and craspedote scolex. The bothridia are separated, with free boarders. The tentacles are cylindrical, armed with solid and similar hooks in quincunxes.
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This paper is the result of adult female mosquito and larval collection in U.S. Army Installations in Korea from 1979 to 1983. New Jersey light traps ere operated for ault collection from May to October. The primary concern of this surveillance is to determine when to recommend insecticide spraying for mosquito control in the Army areas. The 5th Preventive Medicine Unit have developed an "index" level of female mosquitoes in a light trap similar to other U.S.Army Agencies in other parts of the world. When 10 female mosquitoes are reached on two consecutive trap-night, or 5 known vector females are collected, fogging is recommended in the trap areas. Mosquito collections were conducted in 12 U.S. Army areas by operating 39 New Jersey light traps.
Mosquitoes collected from the areas were identified to be 17 species comprising 3 genera. Anopheles sinensis(40%). Culex tritaeniorhyncus(31%), Aedes vexans nipponii(19%), and Culex pipiens pallens(10%) appeared to be the most common species in the area. The species, population density and monthly appearance of adult mosquitoes were found to be almost the same in the all provinces involved. And Japanese encephalitis vector mosquitoes, Culex tritaeniorhyncus, showed their seasonal fluctuation from July to September with a peak in August each year. 3. Larval habitats confirmed in the Army areas were categorized into 16 types as shown in Table The mosquito larvae collected in those habitats were identified to be 15 species representign 4 genera. Most breeding sites in the Army areas were those which are activated during the wet season. More mosquitoes were collected from the Kyungki province than from the other provinces. The reason for more collection of mosquitoes from military installations in the Kyungki provinces appears to be the geographic characteritics surrounded by rice fields, marshes and other stagnant water areas.
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CDC Bottle Bioassays for Detection of Insecticide Resistance in Culex pipiens, Aedes albopictus, and Aedes koreicus Collected on US Army Garrisons, Republic of Korea Craig A. Stoops, Myong Sun Kim, Sanjeev Mahabir, Sung Tae Chong, Stephanie S. Cinkovich, Jennifer B. Carder Journal of the American Mosquito Control Association.2023; 39(3): 208. CrossRef
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Spatial-Temporal Epidemiological Analyses of Two Sympatric, Co-Endemic Alphaviral Diseases in Queensland, Australia Anita M. Pelecanos, Peter A. Ryan, Michelle L. Gatton Vector-Borne and Zoonotic Diseases.2011; 11(4): 375. CrossRef
Late season commercial mosquito trap and host seeking activity evaluation against mosquitoes in a malarious area of the Republic of Korea Douglas A. Burkett, Won-Ja Lee, Kwan-Woo Lee, Heung-Chul Kim, Hee-Il Lee, Jong-Soo Lee, E-Hyun Shin, Robert A. Wirtz, Hae-Wol Cho, David M. Claborn, Russel E. Coleman, Wan Y Kim, Terry A. Klein The Korean Journal of Parasitology.2002; 40(1): 45. CrossRef
Epidemio-entomological survey of Japanese encephalitis in Korea D H Baik, C Y Joo The Korean Journal of Parasitology.1991; 29(1): 67. CrossRef
Seasonal prevalence of the vector mosquitoes of Japanese encephalitis virus in Kyungpook Province, Korea Chong Yoon Joo, Yoshita Wada The Korean Journal of Parasitology.1985; 23(1): 139. CrossRef
In order to estimate the recent pattens of intestinal helminth infections among the residents in Taege City, Korea, a survey based on discovery of helminth eggs by formalin-ether sedimentation and modified Graham's scotch-tape anal swab techniques, were performed during the period from March to September in 1983. A total of 1,697 fecal specimens were collected from 854 male and 843 female residents from 5 villages(Dong), each of whom were selected randomly to represent 1,000 persons referred to the census in 1982. Among the specimens examined, one or more species of helminth parasites were found in 473, revealing the overall positive rate of 27.9 pre cent. Of them, Trichuris trichiura was found most frequently, in 13.2 percent, followed by Enterobius vermicularis in 7.0 per cent.
Hookworm was the least prevalent. The sex-specific rate of overall helminth infections was a little higher in females than in males, while in cases of Clonorchis sinensis, Metagonimus yokogawai and Taenia species, the infection rates in males was higher than in females. T.trichiura was most prevalent in the 20~29 age group and showed about the same prevalence in males and females of all age groups. C.
sinensis infection rate increased with age while those of Ascaris lumbricoides decreased. Single infections were 82.5 per cent of all positive cases. Of cource, T. trichiura revealed the highest prevalence in 31.7 per cent followed by E. vermicularis in 20.1 per cent and A. lumbricoides in 14.8 per cent. Of the double infections, the percentage of T.
trichiura in combination with other intestinal helminths was higher than that of A. lumbricoides. It was concluded that although the infection rates of intestinal helminths among the residents in Taegu City, Korea is still high, eradication of these parasitic diseases seems to be possible with twice-a-year administration of specific anthelmintics, in combination with extensive public health education and improvement of the dietary life.
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Enterobius vermicularis Infection among Preschool Children: A 12-Year (2008-2019) Survey in Large Cities and Provinces of the Republic of Korea Hyejoo Shin, Bong-Kwang Jung, Seungwan Ryoo, Sooji Hong, Taehee Chang, Jiyeon Park, Keon Hoon Lee, Jeonggyu Lee, Jae Young Park, Hoo-Gn Jeoung, Jae Hyun Cho, Jong-Yil Chai The Korean Journal of Parasitology.2021; 59(4): 421. CrossRef
Changing patterns of Clonorchis sinensis infections in Kyongbuk, Korea C Y Joo, M S Chung, S J Kim, C M Kang The Korean Journal of Parasitology.1997; 35(3): 155. CrossRef
The present study was designed to elucidate the recent prevalence of the intestinal protozoa and to demonstrate the relationship on prevalence of the surveyed population with their academic career and living conditions. During the period from May to July 1981, total 2,735 fecal specimens were collected from the inhabitants of 11 areas in Gyeong-gi Do (province) near by Seoul City, and 6 areas in Jeonra Bug Do, southwestern part of Korea. The samples were fixed with SAF solution and were examined by formalin-ether sedimentation technique. The results are summarized as follows: Overall positive rate of intestinal protozoa was 11.1 percent: Gyeong-gi Do, 10.7 percent; Jeonra Bug Do, 11.7 percent. Five species of intestinal protozoa were detected: Entamoeba histolytica 0.69 percent, Entamoeba coli 5.52 percent, Endolimax nana 2.71 percent, Giardia lamblia 2.23 percent, Iodamoeba butschlii 0.l8 percent. Highest positive rates among the examined areas were recognized: Incheon City of Gyeong-gi Do, 26.7 percent; Im-sil Gun(county) of Jeonra Bug Do, 19.9 percent. By species, E.
nana showed the highest prevalence rate (13.74 percent) in Incheon City, and E. histolytica (2.37 percent) and E. coli (9.95 percent) in Im-sil Gun. Prevalence of E. histolytica in rural areas was higher than in urban areas. No significant difference in the prevalence rate by sex and age was recognized, although the group under 10 years and over 60 years were lower than in other age groups. The highest positive rate of E. histolytica was observed in pauper group and significant linearity of prevalence rate of E.
histolytica by monthly income was observed. The inhabitants who used conventional latrine showed higher prevalence, 11.5 percent, than the group of inhabitants who used modified privy. Although no significant difference of positive rate was recognized by academic career, but the lowest prevalence rate was observed in college graduates. Over half of the surveyed population, 64.8 percent has no experience on anthelmintic practice. Number of anthelmintic application resulted no difference in prevalence. In conclusion, prevalences of intestinal protozoa in Gyeong-gi Do and Jeonra Bug Do decreased remarkably in comparison with those of one decade before. However, economic level, academic career and anthelmintic administration seemed no essential reasons for such declination.
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Second report on intestinal parasites among the patients of Seoul Paik Hospital (1984-1992) S K Lee, B M Shin, N S Chung, J Y Chai, S H Lee The Korean Journal of Parasitology.1994; 32(1): 27. CrossRef
Intestinal parasite infections among inhabitants in two islands of Tongyeong-gun, Kyeongsangnam-do S J Hong, H C Woo, J H Han, Y K Seong The Korean Journal of Parasitology.1990; 28(1): 63. CrossRef
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As a series of studies to clarify clonorchicidal substances in body surface mucus of some fresh-water fishes, the substance in the epidermal mucus of Cyprinus carpio was isolated by silica gel column and thin layer chromatography and analyzed for its chemical nature. Wormicidal trial was done in vitro, and the results obtained are summarized as follows: The mucus was extracted by ethyl ether and separated into 4 fractions by column chromatography using benzene as solvent. The second fraction with yellowish red colour among them showed the strongest clonorchicidal effect. The yellowish red fraction obtained by column chromatography was then fractionated into 6 spots by thin layer chromatography with petrol. ether/chloroform(30/70, v/v), and the Rf. 0.714 spot among the 6 spots showed the strongest effect. The Rf. 0.714 spot was further fractionated into 6 spots by thin layer chromatography with benzene/acetone (90/10, v/v), and the Rf. 0.800 spot among the later 6 spots revealed the strongest effect. The Rf.
0.800 spot was chromatographed on column with benzene and 2 fractions were obtained. The second fraction of light brown colour represented the final purified fraction. By these purification procedures, clonorchicidal substance was purified 15-fold with 0.03 percent yield from the mucus of C. carpio, and 10mg of the final fraction killed the cercaria in 26 min, the metacercaria in 115 min, and the adult in 443 min. Infra red and nuclear magnetic resonance spectrometric analysis of the purified substance revealed that the substance belongs to an ethyl ester of unsaturated fatty acid with 2 double bonds, 15 methylene groups and l methyl group.
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An epidemiological study was undertaken to estimate the prevalence of Enterobius vermicularis infection among the primary (and infant) school children in Yongyang-gun, Kyongsangbuk-do. During the period from 2 to 14 December 1983, a total of 2,227 school children in 13 schools were examined one time by means of scotch-tape anal swab technique with a simple questionaire study on perianal itching, enuresis and school record. The results are as follows:The overall egg positive rate was 64.1 percent and the rate ranged from 46.6 to 86.7 percent by schools. The egg positive rates of boys and girls were 60.5 and 68.0 percent respectively and the difference was statistically significant (p<0.005). There was noted a tendency of decrease in prevalence by increase of the age of children.
It was revealed that perianal itching and school record were significantly correlated with E. vermicularis infection (p<0.05) while not in case of enuresis (p>0. l).
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The head louse infestation had been no problem since 1960s in Korea. However, the present study revealed highest infestation rate among the primary school children in Seosan-gun, Chungnam Province in July 1983. The cases of louse positive were determined by identifying the adult worms and/or their nits on scalp and hairs. The overall louse positive rate among 615 children examined was 73.5 percent. The rate was higher in girls (78.8 percent) than in boys (67.6 percent) and was highest in 3rd-4th school grades. It was observed that the more the number of family members the higher the infestation rate of children. The positive children were treated with 20 percent benzyl benzoate solution after the test for louscidal effect in petri dish. However, establishment of intensive control measure is needed to prevent further infestation.
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