The numbers of individual Setaria species in the peritoneal cavities of Korean cattle were estimated. The worms were tentatively identified under light microscopy, and then precisely classified by SEM on the basis of unique features at the anterior and posterior ends of the adult worms. The positive rate of Setaria species was 34.2% out of 1,074 Korean cattle surveyed; that of S. digitata was 25.1%, S.
marshalli 2.9% and both species 6.2%, respectively. Out of a total of 1,254 worms collected, 66.8% were female and 19.1% were male S. digitata ; 9.2% were female and 5.0% were male S. marshalli , respectively. The average length of S.
marshalli was somewhat longer than that of S. digitata. S.
marshalli was documented for the first time in Korea.
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A calf and 50 mice were infected with Cryptosporidium parvum , and their fecal materials were collected and treated with ether extraction (EE), followed by discontinuous sucrose gradients (DSG) method. EE method was to remove some of fat or lipid from feces. Sediments were washed by centrifugation (1,500 x g for 10 min., 3 times) in phosphate-buffered saline and then these washed sediments were sieved sequentially through stainless steel screens with a final mesh of 250 (61 microns porosity) to remove other debris.
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An in vitro culturing to examine the cyst stage of Toxoplasma gondii (ME49 strain) was investigated using murine peritoneal macrophages, and we also examined the effect of cAMP or DHFR inhibitors on the growth of bradyzoites. For experiments ICR mice were injected i.p.
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gondii (ME49 strain) could be maintained and cultivated in vitro by use of murine peritoneal macrophages. In vivo 3 and 5 days and then in vitro 5 and 10 days conditions appeared to be suitable for culturing of bradyzoites. cAMP and pyrimethamine had an effect of stimulation and inhibition on the growth of bradyzoite, respectively.
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This study was designed to evaluate the humoral immune reactions in clonorchiasis before and after praziquantel treatment. Rabbits were infected with 150 or 450 metacercariae, treated on 4 and 8 1/2 months after infection, and observed for 13 months of posttreatment.
Infection controls were maintained for 22 months. Antigen was the metabolic product of worms incubated in physiologic saline. The immune reactions of anti- Clonorchis IgG were observed using SDS-PAGE/immunoblot. During the infection and posttreatment, the antigenic proteins of 66, 63, 54, 52, 50, 47, 42, 40, 38, 34, 33, 30, 27, 25, 23, 20, 19, 18, 17, 16, 15, 14, 13, 12.5 and 11.5 kDa were detected. Of them, 33, 27, 13, and 12.5-kDa antigens were highly antigenic and observed predominantly in infection controls. After the treatment, 13 and 12.5-kDa antigens faded in 6 months after the second treatment, but 33 and 27-kDa antigens were detected until 13 months of posttreatment. The results clearly demonstrate that 13 and 12.5-kDa antigens represent attenuated host immune reactions by praziquantel treatment.
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EXPRESSION OF CYSTEINE PROTEINASE OF CLONORCHIS SINENSIS AND ITS USE IN SERODIAGNOSIS OF CLONORCHIASIS Byoung-Kuk Na, Hye-Jeong Lee, Shin-Hyeong Cho, Hyeong-Woo Lee, Jung-Hwa Cho, Weon-Gyu Kho, Joon-Sang Lee, Jong-Soo Lee, Kyoung-Ju Song, Po-Hyun Park, Chul-Yong Song, Tong-Soo Kim Journal of Parasitology.2002; 88(5): 1000. CrossRef
PURIFICATION AND CHARACTERIZATION OF A 7-KDA PROTEIN FROM CLONORCHIS SINENSIS ADULT WORMS Hye-Jeong Lee, Chang-Seok Lee, Beom-Su Kim, Kyoung-Hwan Joo, Joon-Sang Lee, Tong-Soo Kim, Hak R. Kim Journal of Parasitology.2002; 88(3): 499. CrossRef
Antigenic profile and localization of Clonorchis sinensis proteins in the course of infection Sung-Jong Hong, Tae Yun Kim, Kye-Yong Song, Woon-Mok Sohn, Shin-Yong Kang The Korean Journal of Parasitology.2001; 39(4): 307. CrossRef
Molecular cloning and immunological characterization of phosphoglycerate kinase from Clonorchis sinensis Sung-Jong Hong, Kee-Young Seong, Woon-Mok Sohn, Kye-Yong Song Molecular and Biochemical Parasitology.2000; 108(2): 207. CrossRef
Immunodiagnosis of clonorchiasis using a recombinant antigen T S Yong, H J Yang, S J Park, Y K Kim, D H Lee, S M Lee The Korean Journal of Parasitology.1998; 36(3): 183. CrossRef
A Clonorchis sinensis-specific antigen that detects active human clonorchiasis S I Kim The Korean Journal of Parasitology.1998; 36(1): 37. CrossRef
Immunoblot patterns of clonorchiasis S T Hong, W G Kho, M Lee, J S Lee, S H Lee The Korean Journal of Parasitology.1997; 35(2): 87. CrossRef
The distribution of intestinal capillariasis has been extended continuously through Asian countries. The first case in Korea was reported in 1993, and here we add 2 more cases. One case is a 41-year old man who was diagnosed by both eggs in the feces and worms in the biopsy specimen of the ileum. This case was supposedly infected in Indonesia.
The other is a 78-year old man, who has not been abroad, and suffered from intractable diarrhea. He was diagnosed by eggs in the feces, and several juvenile worms were collected after anthelmintic treatment. In both cases the treatment was successful by albendazole.
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