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Volume 45(1); March 2007

Original Articles

Molecular characterization of bacterial endosymbionts of Acanthamoeba isolates from infected corneas of Korean patients
Ying-Hua Xuan, Hak Sun Yu, Hae Jin Jeong, Sung-Yong Seol, Dong-Il Chung, Hyun-Hee Kong
Korean J Parasitol 2007;45(1):1-9.
Published online March 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.1.1

The endosymbionts of 4 strains of Acanthamoeba (KA/E9, KA/E21, KA/E22, and KA/E23) isolated from the infected corneas of Korean patients were characterized via orcein stain, transmission electron microscopic examination, and 16S rDNA sequence analysis. Double membrane-bound, rod-shaped endosymbionts were distributed randomly throughout both the trophozoites and cysts of each of Acanthamoeba isolates. The endosymbionts of KA/E9, KA/E22, and KA/E23 were surrounded by electron-translucent areas. No lacunae-like structures were observed in the endosymbionts of KA/E21, the bacterial cell walls of which were studded with host ribosomes. Comparative analyses of the 16S rDNA sequences showed that the endosymbionts of KA/E9, KA/E22 and KA/E23 were closely related to Caedibacter caryophilus, whereas the KA/E21 endosymbiont was assigned to the Cytophaga-Flavobacterium-Bacteroides (CFB) phylum. In the 4 strains of Acanthamoeba, the hosts of the endosymbionts were identified as belonging to the Acanthamoeba castellanii complex, which corresponds to the T4 genotype. Acanthamoeba KA/E21 evidenced characteristics almost identical to those of KA/E6, with the exception of the existence of endosymbionts. The discovery of these endosymbionts from Acanthamoeba may prove essential to future studies focusing on interactions between the endosymbionts and the amoebic hosts.

Citations

Citations to this article as recorded by  Crossref logo
  • Proteases of Acanthamoeba
    Behroz Mahdavi Poor, Jalil Rashedi, Vahid Asgharzadeh, Amirali Mirmazhary, Nazila Gheitarani
    Parasitology Research.2024;[Epub]     CrossRef
  • Presence and diversity of free-living amoebae and their potential application as water quality indicators
    Areum Choi, Ji Won Seong, Jeong Hyun Kim, Jun Young Lee, Hyun Jae Cho, Shin Ae Kang, Mi Kyung Park, Mi Jin Jeong, Seo Yeong Choi, Yu Jin Jeong, Hak Sun Yu
    Parasites, Hosts and Diseases.2024; 62(2): 180.     CrossRef
  • Contamination of fresh vegetables in municipal stores with pathogenic Acanthamoeba genotypes; a public health concern
    Marziye Fatemi, Maryam Niyyati, Soheila Rouhani, Seyed Ahmad Karamati, Hamed Mirjalali, Panagiotis Karanis
    International Journal of Environmental Health Research.2023; 33(10): 1010.     CrossRef
  • The life cycle-dependent transcriptional profile of the obligate intracellular amoeba symbiontAmoebophilus asiaticus
    E Selberherr, T Penz, L König, B Conrady, A Siegl, M Horn, S Schmitz-Esser
    FEMS Microbiology Ecology.2022;[Epub]     CrossRef
  • A Systematic Review of Intracellular Microorganisms within Acanthamoeba to Understand Potential Impact for Infection
    Binod Rayamajhee, Dinesh Subedi, Hari Kumar Peguda, Mark Duncan Willcox, Fiona L. Henriquez, Nicole Carnt
    Pathogens.2021; 10(2): 225.     CrossRef
  • Molecular characterization of bacterial, viral and fungal endosymbionts of Acanthamoeba isolates in keratitis patients of Iran
    Elham Hajialilo, Mostafa Rezaeian, Maryam Niyyati, Mohammad Reza Pourmand, Mehdi Mohebali, Mehdi Norouzi, Kobra Razavi Pashabeyg, Sassan Rezaie, Sadegh Khodavaisy
    Experimental Parasitology.2019; 200: 48.     CrossRef
  • Nuclear Group I introns with homing endonuclease genes in Acanthamoeba genotype T4
    Daniele Corsaro, Danielle Venditti
    European Journal of Protistology.2018; 66: 26.     CrossRef
  • Molecular identification of bacterial endosymbionts of Sappinia strains
    Daniele Corsaro, Claudia Wylezich, Julia Walochnik, Danielle Venditti, Rolf Michel
    Parasitology Research.2017; 116(2): 549.     CrossRef
  • Identification of Paenibacillus as a Symbiont in Acanthamoeba
    Vinicius José Maschio, Gertrudes Corção, Francielle Bücker, Karin Caumo, Marilise Brittes Rott
    Current Microbiology.2015; 71(3): 415.     CrossRef
  • Identifying endosymbiont bacteria associated with free‐living amoebae
    Pilar Goñi, María Teresa Fernández, Encarnación Rubio
    Environmental Microbiology.2014; 16(2): 339.     CrossRef
  • Acanthamoeba castellanii cysts: new ultrastructural findings
    Bibiana Chávez-Munguía, Lizbeth Salazar-Villatoro, Anel Lagunes-Guillén, Maritza Omaña-Molina, Martha Espinosa-Cantellano, Adolfo Martínez-Palomo
    Parasitology Research.2013; 112(3): 1125.     CrossRef
  • Functional expression and characterization of an iron-containing superoxide dismutase of Acanthamoeba castellanii
    Jung-Yeon Kim, Byoung-Kuk Na, Kyoung-Ju Song, Mi-Hyun Park, Yun-Kyu Park, Tong-Soo Kim
    Parasitology Research.2012; 111(4): 1673.     CrossRef
  • A bacterial genome in transition - an exceptional enrichment of IS elements but lack of evidence for recent transposition in the symbiont Amoebophilus asiaticus
    Stephan Schmitz-Esser, Thomas Penz, Anja Spang, Matthias Horn
    BMC Evolutionary Biology.2011;[Epub]     CrossRef
  • Is Acanthamoeba pathogenicity associated with intracellular bacteria?
    Graeme Neil Paterson, Michael Rittig, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
    Experimental Parasitology.2011; 129(2): 207.     CrossRef
  • Detection of Bacterial Endosymbionts in Clinical Acanthamoeba Isolates
    Alfonso Iovieno, Dolena R. Ledee, Darlene Miller, Eduardo C. Alfonso
    Ophthalmology.2010; 117(3): 445.     CrossRef
  • Bilateral Acanthamoeba Keratitis After Orthokeratology
    Eun Chul Kim, Man Soo Kim
    Cornea.2010; 29(6): 680.     CrossRef
  • Free-living amoebae and their intracellular pathogenic microorganisms: risks for water quality
    Vincent Thomas, Gerald McDonnell, Stephen P. Denyer, Jean-Yves Maillard
    FEMS Microbiology Reviews.2010; 34(3): 231.     CrossRef
  • Bilateral Acanthamoeba Keratitis After Orthokeratology
    Eun Chul Kim, Man Soo Kim
    Cornea.2009; 28(3): 348.     CrossRef
  • Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates
    Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung
    The Korean Journal of Parasitology.2008; 46(3): 157.     CrossRef
  • Diversity of Bacterial Endosymbionts of Environmental Acanthamoeba Isolates
    Stephan Schmitz-Esser, Elena R. Toenshoff, Susanne Haider, Eva Heinz, Verena M. Hoenninger, Michael Wagner, Matthias Horn
    Applied and Environmental Microbiology.2008; 74(18): 5822.     CrossRef
  • 10,049 View
  • 94 Download
  • Crossref
Natural occurrence of Mycobacterium as an endosymbiont of Acanthamoeba isolated from a contact lens storage case
Hak Sun Yu, Hae Jin Jeong, Yeon-Chul Hong, Seong-Yong Seol, Dong-Il Chung, Hyun-Hee Kong
Korean J Parasitol 2007;45(1):11-18.
Published online March 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.1.11

Recent in vitro studies have revealed that a certain Mycobacterium can survive and multiply within free-living amoebae. It is believed that protozoans function as host cells for the intracellular replication and evasion of Mycobacterium spp. under harmful conditions. In this study, we describe the isolation and characterization of a bacterium naturally observed within an amoeba isolate acquired from a contact lens storage case. The bacterium multiplied within Acanthamoeba, but exerted no cytopathic effects on the amoeba during a 6-year amoebic culture. Trasnmission electron microscopy showed that the bacteria were randomly distributed within the cytoplasm of trophozoites and cysts of Acanthamoeba. On the basis of the results of 18S rRNA gene analysis, the amoeba was identified as A. lugdunensis. A 16S rRNA gene analysis placed this bacterium within the genus Mycobacterium. The bacterium evidenced positive reactivity for acid-fast and fluorescent acid-fast stains. The bacterium was capable of growth on the Middlebrook 7H11-Mycobacterium-specific agar. The identification and characterization of bacterial endosymbionts of free-living protozoa bears significant implications for our understanding of the ecology and the identification of other atypical mycobacterial pathogens.

Citations

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  • A Comparative Genomic Approach to Determine the Virulence Factors and Horizontal Gene Transfer Events of Clinical Acanthamoeba Isolates
    Xiaobin Gu, Xiuhai Lu, Shudan Lin, Xinrui Shi, Yue Shen, Qingsong Lu, Yiying Yang, Jing Yang, Jiabei Cai, Chunyan Fu, Yongliang Lou, Meiqin Zheng, Tim Downing, Kirti Megha
    Microbiology Spectrum.2022;[Epub]     CrossRef
  • A Systematic Review of Intracellular Microorganisms within Acanthamoeba to Understand Potential Impact for Infection
    Binod Rayamajhee, Dinesh Subedi, Hari Kumar Peguda, Mark Duncan Willcox, Fiona L. Henriquez, Nicole Carnt
    Pathogens.2021; 10(2): 225.     CrossRef
  • Detection of Free-Living Amoebae and Their Intracellular Bacteria in Borehole Water before and after a Ceramic Pot Filter Point-of-Use Intervention in Rural Communities in South Africa
    Clarissa van der Loo, Catheleen Bartie, Tobias George Barnard, Natasha Potgieter
    International Journal of Environmental Research and Public Health.2021; 18(8): 3912.     CrossRef
  • The key factors contributing to the risk, diagnosis and treatment of non-tuberculous mycobacterial opportunistic infections
    Anna Grzegorzewicz, Mariola Paściak
    Postępy Higieny i Medycyny Doświadczalnej.2021; 75(1): 696.     CrossRef
  • Co-Existence of Free-Living Amoebae and Potential Human Pathogenic Bacteria Isolated from Rural Household Water Storage Containers
    Natasha Potgieter, Clarissa van der Loo, Tobias George Barnard
    Biology.2021; 10(12): 1228.     CrossRef
  • The core microbiome of sessile ciliate Stentor coeruleus is not shaped by the environment
    Olivia Lanzoni, Andrey Plotnikov, Yuri Khlopko, Giulio Munz, Giulio Petroni, Alexey Potekhin
    Scientific Reports.2019;[Epub]     CrossRef
  • Repertoire of free-living protozoa in contact lens solutions
    Ibtissem Bouchoucha, Aurore Aziz, Louis Hoffart, Michel Drancourt
    BMC Ophthalmology.2016;[Epub]     CrossRef
  • Current and Past Strategies for Bacterial Culture in Clinical Microbiology
    Jean-Christophe Lagier, Sophie Edouard, Isabelle Pagnier, Oleg Mediannikov, Michel Drancourt, Didier Raoult
    Clinical Microbiology Reviews.2015; 28(1): 208.     CrossRef
  • Looking in amoebae as a source of mycobacteria
    M. Drancourt
    Microbial Pathogenesis.2014; 77: 119.     CrossRef
  • Molecular characterization and ultrastructure of a new amoeba endoparasite belonging to the Stenotrophomonas maltophilia complex
    Daniele Corsaro, Karl-Dieter Müller, Rolf Michel
    Experimental Parasitology.2013; 133(4): 383.     CrossRef
  • Identification of Free-Living Amoebae and Amoeba-Associated Bacteria from Reservoirs and Water Treatment Plants by Molecular Techniques
    Alicia Garcia, Pilar Goñi, Joanna Cieloszyk, Maria Teresa Fernandez, Laura Calvo-Beguería, Encarnacion Rubio, Maria Francisca Fillat, Maria Luisa Peleato, Antonio Clavel
    Environmental Science & Technology.2013; 47(7): 3132.     CrossRef
  • Cooccurrence of Free-Living Amoebae and Nontuberculous Mycobacteria in Hospital Water Networks, and Preferential Growth of Mycobacterium avium in Acanthamoeba lenticulata
    Alida R. Ovrutsky, Edward D. Chan, Marinka Kartalija, Xiyuan Bai, Mary Jackson, Sara Gibbs, Joseph O. Falkinham, Michael D. Iseman, Paul R. Reynolds, Gerald McDonnell, Vincent Thomas
    Applied and Environmental Microbiology.2013; 79(10): 3185.     CrossRef
  • Detection of Bacterial Endosymbionts in Clinical Acanthamoeba Isolates
    Alfonso Iovieno, Dolena R. Ledee, Darlene Miller, Eduardo C. Alfonso
    Ophthalmology.2010; 117(3): 445.     CrossRef
  • Biodiversity of amoebae and amoeba-associated bacteria in water treatment plants
    Daniele Corsaro, Gemma Saucedo Pages, Vicente Catalan, Jean-François Loret, Gilbert Greub
    International Journal of Hygiene and Environmental Health.2010; 213(3): 158.     CrossRef
  • Free-living amoebae and their intracellular pathogenic microorganisms: risks for water quality
    Vincent Thomas, Gerald McDonnell, Stephen P. Denyer, Jean-Yves Maillard
    FEMS Microbiology Reviews.2010; 34(3): 231.     CrossRef
  • Free-living amoebae, a training field for macrophage resistance of mycobacteria
    I.B. Salah, E. Ghigo, M. Drancourt
    Clinical Microbiology and Infection.2009; 15(10): 894.     CrossRef
  • Endosymbionts of Acanthamoeba Isolated from Domestic Tap Water in Korea
    Seon Hee Choi, Min Kyoung Cho, Soon Cheol Ahn, Ji Eun Lee, Jong Soo Lee, Dong-Hee Kim, Ying-Hua Xuan, Yeon Chul Hong, Hyun Hee Kong, Dong Il Chung, Hak Sun Yu
    The Korean Journal of Parasitology.2009; 47(4): 337.     CrossRef
  • Survival of amoebae on building materials
    T. Yli-Pirilä, J. Kusnetsov, M.-R. Hirvonen, M. Seuri, A. Nevalainen
    Indoor Air.2009; 19(2): 113.     CrossRef
  • Occurrence of Free-Living Amoebae in Communities of Low and High Endemicity for Buruli Ulcer in Southern Benin
    Miriam Eddyani, Johan F. De Jonckheere, Lies Durnez, Patrick Suykerbuyk, Herwig Leirs, Françoise Portaels
    Applied and Environmental Microbiology.2008; 74(21): 6547.     CrossRef
  • Relationship between mycobacteria and amoebae: ecological and epidemiological concerns
    V. Thomas, G. McDonnell
    Letters in Applied Microbiology.2007; 45(4): 349.     CrossRef
  • 10,874 View
  • 97 Download
  • Crossref
Characterization of a Toxocara canis species-specific excretory-secretory antigen (TcES-57) and development of a double sandwich ELISA for diagnosis of visceral larva migrans
R.D. Iddawela, R.P.V.J. Rajapakse, N.A.N.D. Perera, Takeshi Agatsuma
Korean J Parasitol 2007;45(1):19-26.
Published online March 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.1.19

This study describes the isolation of a Toxocara canis species-specific excretory-secretory (ES) antigen and the development of an enzyme-linked immunosorbent assay (ELISA) based on this antigen. Analysis of the ES antigens of T. canis, Toxocara vitulorum, Ascaris lumbricoides and Necator americanus larval antigen was performed by SDS-PAGE followed by western blotting. A 57 kDa T. canis-specific antibody fraction (TcES-57) was identified by western blotting and labelling with anti-Toxocara antibodies (from experimental rabbits and human patients) and tracing with anti-human or anti-rabbit peroxidase conjugate. No protein fraction of 57 kDa was detected in ES or larval antigens collected from T. canis, T. vitulorum, A. lumbricoides and N. americanus. Using TcES-57, a specific anti-serum was produced in rabbits and a double sandwich ELISA was developed. This test was validated using known seropositive sera from toxocariasis patients, sera from A. lumbricoides or N. americanus patients, and 50 serum samples from cats. These tests revealed that TcES-57 antigen is specific to T. canis infection and does not cross react with sera of other related infections. Thus, ELISA based on TcES-57 antigen was proven to be an effective tool in the diagnosis of toxocariasis and studies on the role of T. canis in the epidemiology of human toxocariasis.

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  • Two trichinellosis outbreaks in Serbia – challenging diagnosis due to a potential co-infection with Toxocara spp.
    I. Mitic, M. Gnjatovic, S. Vasilev, N. Ristovic, N. Miladinovic-Tasic, Lj. Sofronic-Milosavljevic
    Journal of Helminthology.2022;[Epub]     CrossRef
  • Cysticercus cellulosae Regulates T-Cell Responses and Interacts With the Host Immune System by Excreting and Secreting Antigens
    Xianmin Fan, Yue Zhang, Renhui Ouyang, Bo Luo, Lizhu Li, Wei He, Meichen Liu, Nan Jiang, Fengjiao Yang, Lingjun Wang, Biying Zhou
    Frontiers in Cellular and Infection Microbiology.2021;[Epub]     CrossRef
  • Frequency ofToxocara canisantibodies in Mexican paediatric patients with epilepsy
    M.d.L. Caballero-García, J. Simón-Salvador, J.C. Hernández-Aguilar, A. Reyes-Lopez, B. Nogueda-Torres, E. Jiménez-Cardoso
    Journal of Helminthology.2020;[Epub]     CrossRef
  • Immunodiagnosis of anti-Toxocara vitulorum IgG antibodies by using commercially available bovine ELISA Kit in bovine of Potohar region Pakistan
    Samina Asghar Abbasi, Mazhar Qayum, Ruqayya Mehmood Baig, Mehvish Naseer Ahmad, Akbar Shah, Mujeeb ur Rahman, Haroon
    Acta Ecologica Sinica.2020; 40(1): 97.     CrossRef
  • Toxocara canis infection may impair bovine herpesvirus type 5 immunization
    Y.A. Menegon, N.B. Pinheiro, L.M. Santos, P.R.C. Rodrigues, L.F.C. Avila, F.R. Conceição, F.P.L. Leite
    Research in Veterinary Science.2020; 132: 268.     CrossRef
  • An innovative approach in the detection of Toxocara canis excretory/secretory antigens using specific nanobodies
    Francisco J. Morales-Yanez, Idalia Sariego, Cécile Vincke, Gholamreza Hassanzadeh-Ghassabeh, Katja Polman, Serge Muyldermans
    International Journal for Parasitology.2019; 49(8): 635.     CrossRef
  • Toxocara related peritonitis: A case report and review of literature
    Ferhat Arslan, Naciye Betul Baysal, Ahmet Aslan, Bengu Cobanoglu Simsek, Haluk Vahaboglu
    Parasitology International.2019; 73: 101950.     CrossRef
  • Toxocara Seroprevalence and Associated Risk Factors Among Ilam Children, West of Iran
    Saleh Khoshnood, Nader Pestehchian, Seyed Hossein Hejazi, Abdollah Rafiei, Ali Soleimani, Mahmoud Ahmadi, Zahra Jabalameli, Mohammad Ali Mohaghegh
    Archives of Pediatric Infectious Diseases.2018;[Epub]     CrossRef
  • Proteomic analysis of Toxocara canis excretory and secretory (TES) proteins
    Rita Leal Sperotto, Frederico Schmitt Kremer, Maria Elisabeth Aires Berne, Luciana F. Costa de Avila, Luciano da Silva Pinto, Karina Mariante Monteiro, Karin Silva Caumo, Henrique Bunselmeyer Ferreira, Natália Berne, Sibele Borsuk
    Molecular and Biochemical Parasitology.2017; 211: 39.     CrossRef
  • Sero-detection of Toxocara canis infection in human with T.canis recombinant arginine kinase, cathepsin L-1 and TES-26 antigens
    Anju Varghese, Opinder K. Raina, Dinesh Chandra, Bijay R. Mirdha, Naresh H. Kelawala, Jayesh B. Solanki, Niranjan Kumar, Reghu Ravindran, Anandanarayanan Arun, Ajayta Rialch, Hniang Lalrinkima, Rohan N. Kelawala, Subhamoy Samanta
    Acta Parasitologica.2017;[Epub]     CrossRef
  • In vitro production of Toxocara canis excretory-secretory (TES) antigen
    Divyamol Thomas, N. Jeyathilakan, S. Abdul Basith, T. M. A. Senthilkumar
    Journal of Parasitic Diseases.2016; 40(3): 1038.     CrossRef
  • Development of a Luminex Bead Based Assay for Diagnosis of Toxocariasis Using Recombinant Antigens Tc-CTL-1 and Tc-TES-26
    John P. Anderson, Lisa N. Rascoe, Keith Levert, Holly M. Chastain, Matthew S. Reed, Hilda N. Rivera, Isabel McAuliffe, Bin Zhan, Ryan E. Wiegand, Peter J. Hotez, Patricia P. Wilkins, Jan Pohl, Sukwan Handali, Gustavo Schmidt Moreira
    PLOS Neglected Tropical Diseases.2015; 9(10): e0004168.     CrossRef
  • Characterization of excretory-secretory antigens of adult Toxocara canis by western blotting
    N. R. Sudhakar, S. Samanta, Shivani Sahu, O. K. Raina, S. C. Gupta, T. K. Goswami, K. M. Lokesh, Ashok Kumar
    Journal of Parasitic Diseases.2014; 38(2): 166.     CrossRef
  • Bladder and Liver Involvement of Visceral Larva Migrans May Mimic Malignancy
    Eun Joo Kang, Yoon Ji Choi, Jung Sun Kim, Byung Hyun Lee, Ka-Won Kang, Hong Jun Kim, Eun Sang Yu, Yeul Hong Kim
    Cancer Research and Treatment.2014; 46(4): 419.     CrossRef
  • In vitro culture of Parascaris equorum larvae and initial investigation of parasite excretory-secretory products
    Steffanie V. Burk, Sriveny Dangoudoubiyam, Tammy Brewster-Barnes, Uneeda K. Bryant, Daniel K. Howe, Craig N. Carter, Eric S. Vanzant, Robert J. Harmon, Kevin R. Kazacos, Mary G. Rossano
    Parasitology Research.2014; 113(11): 4217.     CrossRef
  • Evaluación de un antígeno purificado para el diagnóstico de toxocariosis
    Graciela Santillán, Vanesa Bastin, Graciela Céspedes, Adriana Monkiewicz
    Revista Argentina de Microbiología.2013; 45(2): 80.     CrossRef
  • Seroprevalence and risk factors for Toxocara infection in children from an urban large setting in Northeast Brazil
    Lívia R. Mendonça, Camila A. Figueiredo, Renata Esquivel, Rosemeire L. Fiaccone, Lain Pontes-de-Carvalho, Phillip Cooper, Maurício L. Barreto, Neuza M. Alcantara-Neves
    Acta Tropica.2013; 128(1): 90.     CrossRef
  • Seroprevalence ofToxocaraInfection in Children from Southern Brazil
    Elizandra R. Schoenardie, Carlos J. Scaini, Claudiomar S. Brod, Michele S. Pepe, Marcos M. Villela, Alan J. A. McBride, Sibele Borsuk, Maria E. A. Berne
    Journal of Parasitology.2013; 99(3): 537.     CrossRef
  • Evaluation of an enzyme-linked immunoelectrotransfer blot test for the confirmatory serodiagnosis of human toxocariasis
    William H Roldán, Yrma A Espinoza
    Memórias do Instituto Oswaldo Cruz.2009; 104(3): 411.     CrossRef
  • Update on Eosinophilic Meningoencephalitis and Its Clinical Relevance
    Carlos Graeff-Teixeira, Ana Cristina Arámburu da Silva, Kentaro Yoshimura
    Clinical Microbiology Reviews.2009; 22(2): 322.     CrossRef
  • Seroprevalence of Toxocariasis among Healthy People with Eosinophilia
    Yong-Hun Kim, Sun Huh, Young-Bae Chung
    The Korean Journal of Parasitology.2008; 46(1): 29.     CrossRef
  • Serodiagnosis of Human Toxocariasis Using Adult Somatic and Excretory-Secretory Antigens of Toxocara canis
    Nawal A. Hassanain ., Mona S. Mahmoud .
    Research Journal of Parasitology.2008; 3(3): 85.     CrossRef
  • Development of a highly sensitive IgG-ELISA based on recombinant arginine kinase of Toxocara canis for serodiagnosis of visceral larva migrans in the murine model
    Susiji Wickramasinghe, Lalani Yatawara, Mitsuru Nagataki, Misa Takamoto, Yoshiya Watanabe, R. P. V. J. Rajapakse, Kouji Uda, Tomohiko Suzuki, Takeshi Agatsuma
    Parasitology Research.2008; 103(4): 853.     CrossRef
  • A case of adult hepatic toxocariasis
    Jared Hossack, Paul Ricketts, Helen S Te, John Hart
    Nature Clinical Practice Gastroenterology & Hepatology.2008; 5(6): 344.     CrossRef
  • An improved method to obtain antigen-excreting Toxocara canis larvae
    Neuza Maria Alcântara-Neves, Alex Barbosa dos Santos, Lívia Ribeiro Mendonça, Camila A.V. Figueiredo, Lain Pontes-de-Carvalho
    Experimental Parasitology.2008; 119(3): 349.     CrossRef
  • 9,671 View
  • 114 Download
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A serological survey of Dirofilaria immitis infection in pet dogs of Busan, Korea, and effects of chemoprophylaxis
Kang Hyun Byeon, Bong Jin Kim, Sun-Mi Kim, Hak Sun Yu, Hae Jin Jeong, Mee-Sun Ock
Korean J Parasitol 2007;45(1):27-32.
Published online March 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.1.27

The status of Dirofilaria immitis infection was assessed in pet dogs of Busan, Korea, and chemoprophylactic effects of microfilaricidal medication were evaluated. A total of 294 pet dogs older than 6 mo were examined, 217 of which had been maintained indoors, and 77 had been kept outdoors. The SnapR kit and direct microscopic examinations of the peripheral blood were used. The mean overall parasite positive rates were 10.2% and 6.5%, respectively. Outdoor dogs evidenced adult worm infection rate of 31.2% and microfilaria infection rate of 18.2%. The indoor dogs, however, evidenced adult worm infection rate of 2.8% and microfilaria infection rate of 2.3%. The prevalence in males was more than 2 times that of females. The changing pattern of infection rates by age evidenced a gradual increase, from 2- to 6-year-old dogs, after which, a decrease in infection rates was noted. With regard to chemoprophylaxis, the infection rates of complete and incomplete chemoprophylaxis groups were found to be 2-3 times lower than that of the non-chemoprophylaxis group. The results of the present study indicate that the risk of exposure to D. immitis in pet dogs is quite high, particularly in male outdoor dogs, and chemoprophylactic measures were quite effective.

Citations

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  • Filariasis in dogs brought to the Veterinary Teaching Hospital, University of Peradeniya, Sri Lanka
    Sandani S. Thilakarathne, Nayana Wijayawardhane, Piyumali K. Perera, Chandima Mallawa, Rupika S. Rajakaruna
    Parasitology Research.2023; 122(2): 461.     CrossRef
  • Molecular detection of Dirofilaria immitis and its Wolbachia endosymbionts in dogs from Myanmar
    Saw Bawm, Yadanar Khaing, Hla Myet Chel, Myint Myint Hmoon, Shwe Yee Win, Min Bo, Tint Naing, Lat Lat Htun
    Current Research in Parasitology & Vector-Borne Diseases.2023; 4: 100148.     CrossRef
  • Detection of Dirofilaria immitis using microscopic, serological and molecular techniques among dogs in Cabo Frio, RJ, Brazil
    Taíssa Angélica Lemos Trancoso, Nathália da Conceição Lima, Alynne Silva Barbosa, Daniela Leles, Ana Beatriz Monteiro Fonseca, Norma Vollmer Labarthe, Otilio Machado Pereira Bastos, Claudia Maria Antunes Uchôa
    Revista Brasileira de Parasitologia Veterinária.2020;[Epub]     CrossRef
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  • Incidence of Dirofilaria immitis in dogs presented at University of Nigeria, Nsukka Veterinary Teaching Hospital using wet smear and buffy coat techniques
    Chukwuebuka Iniobong Ikenna Ugochukwu, Nebolisah Omekam, Emmanuel Ikenna Ugochukwu
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  • Prevalence ofDirofilaria immitisinfection in dogs in Henan province, central China
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  • Epidemiological aspects of Dirofilaria immitis infection in dogs from Assam of Northeast India
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A new endemic focus of Heterophyes nocens and other heterophyid infections in a coastal area of Gangjin-gun, Jeollanam-do
Jae-Hwan Park, Jae-Lip Kim, Eun-Hee Shin, Sang-Mee Guk, Yun-Kyu Park, Jong-Yil Chai
Korean J Parasitol 2007;45(1):33-38.
Published online March 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.1.33

To know the prevalence of heterophyid trematodes among inhabitants of a southern coastal village, i.e., Sacho-ri, Gangjin-gun, Jeollanam-do (Province), 82 stool samples were examined on helminth eggs and protozoan cysts using Kato-Katz and formalin-ether sedimentation techniques. Total 33 people (40.2%) were positive for trematodes (Heterophyes nocens; 15 people, Pygidiopsis summa; 3, Metagonimus sp.; 4, Clonorchis sinensis; 7, Gymnophalloides seoi; 6) and/or protozoa (Entamoeba coli; 3). Among intestinal trematode egg positive cases, 17 were treated with praziquantel and their whole diarrheic stools were collected after purgation. Adult flukes of H. nocens (number of specimens = 1,294), P. summa (386), Stellantchasmus falcatus (5), Stictodora lari (4), and Heterophyopsis continua (1) were collected using a stereomicroscope. To know the source of human H. nocens infections in this village, metacercarial infections in mullets (10) were examined and most H. nocens metacercariae (101/105, 96.2%) were found in the trunk portion. From above results, the surveyed coastal village has been newly known as an endemic focus of human H. nocens infection and consuming raw mullets was the presumable source of human heterophyid infections.

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  • Infection characteristics of Metagonimus species (Digenea: Heterophyidae) metacercariae in fish from major rivers of Korea
    Woon-Mok Sohn
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    Woon-Mok Sohn
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  • Infection Status with Clonorchis sinensis Metacercariae in Fish from Tamjin-gang (River) in Jeollanam-do, Republic of Korea
    Ki-Bok Yoon, Hyun-Cheol Lim, Doo Young Jeon, Sook Park, Shin-Hyeong Cho, Jung-Won Ju, Sung-Shik Shin, Byoung-Kuk Na, Woon-Mok Sohn
    The Korean Journal of Parasitology.2018; 56(2): 183.     CrossRef
  • Infections with Zoonotic Trematode Metacercariae in Yellowfin Goby, Acanthogobius flavimanus, from Coastal Areas of Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Jung-Won Ju, Soon-Won Lee, Won-Seok Seok
    The Korean Journal of Parasitology.2018; 56(3): 259.     CrossRef
  • Infection Status with Metagonimus spp. Metacercariae in Fishes from Seomjin-gang and Tamjin-gang in Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Jung-Won Ju, Cheon-Hyeon Kim, Ki-Bok Yoon
    The Korean Journal of Parasitology.2018; 56(4): 351.     CrossRef
  • Fishborne zoonotic heterophyid infections: An update
    Jong-Yil Chai, Bong-Kwang Jung
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  • Pygidiopsis summa (Digenea: Heterophyidae): Status of Metacercarial Infection in Mullets from Coastal Areas in the Republic of Korea
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    The Korean Journal of Parasitology.2016; 54(4): 497.     CrossRef
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  • Infection Status of Estuarine Fish and Oysters with Intestinal Fluke Metacercariae in Muan-gun, Jeollanam-do, Korea
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    The Korean Journal of Parasitology.2010; 48(2): 133.     CrossRef
  • Foodborne Intestinal Flukes in Southeast Asia
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    The Korean Journal of Parasitology.2009; 47(Suppl): S69.     CrossRef
  • Trends in parasitic diseases in the Republic of Korea
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    Trends in Parasitology.2008; 24(3): 143.     CrossRef
  • Emerging Parasitic Diseases in Korea
    Jong-Yil Chai
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A new endemic focus of Gymnophalloides seoi infection on Aphae Island, Shinan-gun, Jeollanam-do
Jae-Hwan Park, Sang-Mee Guk, Eun-Hee Shin, Hyo-Jin Kim, Jae-Lip Kim, Min Seo, Yun-Kyu Park, Jong-Yil Chai
Korean J Parasitol 2007;45(1):39-44.
Published online March 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.1.39

A new endemic focus of Gymnophalloides seoi infection has been discovered on Aphae Island (Shinan-gun, Jeollanam-do), Republic of Korea. This area, which is referred to as Bokyong-ri, is a small seashore village located in the northern portion of the island. Fecal samples were collected from a total of 57 residents and examined by the Kato-Katz and formalin-ether sedimentation techniques. Helminth eggs were detected in 37 samples (64.9%); 33 samples were positive for G. seoi eggs (57.9%), 4 for Pygidiopsis summa (7.0%), 13 for other heterophyids (22.8%), 1 for Clonorchis sinensis (1.7%), and 1 for Trichuris trichiura (1.7%). Women (70.4%) revealed higher rates of G. seoi infection than did men (46.7%), and individuals older than 50 years of age also evidenced higher rates of G. seoi infection than in other age groups (P< 0.05). In worm collection from 13 G. seoi egg positive cases, G. seoi (total 86,558 specimens), Heterophyes nocens (278), Stictodora sp. (10), Heterophyopsis continua (3), P. summa (3), and unidentified flukes (42) were collected. Oysters, the source of G. seoi infection, were collected from an area proximal to the village and 50 were examined for metacercariae; 47 (94%) were found infected and the observed metacercarial density was 9.5 ± 8.9 per oyster. The results of this study indicate that Bokyong-ri is a new endemic area of G. seoi infection, with high rates of infection in humans and oysters.

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  • Survey of Gymnophalloides seoi Metacercariae in Natural and Cultured Oysters from Several Western Coastal Areas, Korea
    Taehee Chang, Bong-Kwang Jung, Hyemi Song, Jaeeun Cho, Sooji Hong, Keon-Hoon Lee, Eui-Hyug Hoang, Jisu Kang, Jini Lim, Hana Lee, Jong-Yil Chai
    The Korean Journal of Parasitology.2019; 57(6): 705.     CrossRef
  • Pygidiopsis summa (Digenea: Heterophyidae): Status of Metacercarial Infection in Mullets from Coastal Areas in the Republic of Korea
    Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Won-Ja Lee, Mi-Yeoun Park, Soon-Won Lee, Seung-Bong Choi, Beom-Nyung Huh, Won-Seok Seok
    The Korean Journal of Parasitology.2016; 54(4): 497.     CrossRef
  • Korean molluscs as auxiliary hosts for parasites: A study of implications for pathogen transmission in a changing climate
    Gab-Man Park
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  • Emerging Parasitic Diseases in Korea
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Survey for zoonotic liver and intestinal trematode metacercariae in cultured and wild fish in An Giang Province, Vietnam
Nguyen Diem Thu, Anders Dalsgaard, Ly Thi Thanh Loan, K. Darwin Murrell
Korean J Parasitol 2007;45(1):45-54.
Published online March 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.1.45

Although Vietnam has a high risk of fishborne zoonotic trematode (FZT) infections for humans, little information exists on the epidemiology of these infections in the country's fish. Because of the importance of cultured catfish and snakehead production in An Giang province, a major production area in the Mekong Delta of Vietnam, a survey for FZTs was carried out in randomly selected fish farms between June 2005 and March 2006. For comparison, wild fish from the same area were also surveyed. A total of 852 cultured fish from 4 districts were collected and examined by pepsin digestion to determine their FZT infection status. In Tra catfish, the prevalence of all types of metacercariae was 2.6%, of which the prevalence of Haplorchis pumilio was 0.7%. The overall prevalence of metacercariae in wild fish was 30.6%, of which 10.3% harbored zoonotic species: H. pumilio (2.8%) and Procerovum sp. (5.6%). The prevalence of Opisthorchis metacercariae, which were diagnosed as O. viverrini, was 1.9%. No metacercariae were found in cultured snakehead fish, although wild-caught snakehead fish had a FZT prevalence of 10.3%: 5.1% were O. viverrini; 2.6% H. pumilio; and 2.6% were Procerovum sp. These are the first reports of H. pumilio, Procerovum sp., and O. viverrini metacercariae in Vietnamese fish. These results indicate that consumption of improperly prepared fish represents a significant risk of acquiring FZTs in this south Vietnam region.

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  • Three species of Exorchis Kobayashi, 1921 (Digenea: Cryptogonimidae) in the East-Asian region: morphological and molecular data
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  • Fish-borne trematode infections in wild fishes in Bangladesh
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  • Seasonal prevalence of zoonotic trematode parasites in commercial catfish species in the Ho Chi Minh City area
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  • Fish substitutions which may increase human health risks from zoonotic seafood borne parasites: A review
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  • High Prevalence of Clonorchis sinensis and Other Zoonotic Trematode Metacercariae in Fish from a Local Market in Yen Bai Province, Northern Vietnam
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    The Korean Journal of Parasitology.2020; 58(3): 333.     CrossRef
  • Zoonotic parasites carried by invasive alien species in China
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  • Infections by Hepatic Trematodes in Cats from Slaughterhouses in Vietnam
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  • Clonorchis sinensisandOpisthorchisspp. in Vietnam: current status and prospects
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    James V. Conlan, Banchob Sripa, Stephen Attwood, Paul N. Newton
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    Van Thi Phan, Annette Kjær Ersbøll, Te Quang Bui, Hang Thi Nguyen, Darwin Murrell, Anders Dalsgaard
    Vector-Borne and Zoonotic Diseases.2010; 10(9): 861.     CrossRef
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    Acta Tropica.2010; 116(1): 15.     CrossRef
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    Dinh Thi Thuy, Per Kania, Kurt Buchmann
    Aquaculture.2010; 302(1-2): 19.     CrossRef
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    The Korean Journal of Parasitology.2010; 48(3): 225.     CrossRef
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    Nguyen Thi Lan Anh, Henry Madsen, Anders Dalsgaard, Nguyen Thi Phuong, Dao Thi Ha Thanh, K. Darwin Murrell
    Veterinary Parasitology.2010; 169(3-4): 391.     CrossRef
  • Freshwater Aquaculture Nurseries and Infection of Fish with Zoonotic Trematodes, Vietnam
    Van Thi Phan, Annette Kjær Ersbøll, Thanh Thi Nguyen, Khue Viet Nguyen, Ha Thi Nguyen, Darwin Murrell, Anders Dalsgaard
    Emerging Infectious Diseases.2010; 16(12): 1905.     CrossRef
  • Farm-Level Risk Factors for Fish-Borne Zoonotic Trematode Infection in Integrated Small-Scale Fish Farms in Northern Vietnam
    Van Thi Phan, Annette Kjær Ersbøll, Khue Viet Nguyen, Henry Madsen, Anders Dalsgaard, Banchob Sripa
    PLoS Neglected Tropical Diseases.2010; 4(7): e742.     CrossRef
  • Survey for Fishborne Zoonotic Metacercariae in Farmed Grouper in Vietnam
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    Foodborne Pathogens and Disease.2009; 6(8): 1037.     CrossRef
  • Prevalence and risks for fishborne zoonotic trematode infections in domestic animals in a highly endemic area of North Vietnam
    Nguyen Thi Lan Anh, Nguyen Thi Phuong, Maria Vang Johansen, K. Darwin Murrell, Phan Thi Van, Anders Dalsgaard, Luong To Thu, Stig Milan Thamsborg
    Acta Tropica.2009; 112(2): 198.     CrossRef
  • Prevalence of zoonotic trematode parasites in fish fry and juveniles in fish farms of the Mekong Delta, Vietnam
    Cu Pham Thien, Anders Dalsgaard, Nguyen Thanh Nhan, Annette Olsen, K. Darwin Murrell
    Aquaculture.2009; 295(1-2): 1.     CrossRef
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    Veterinary Parasitology.2009; 160(1-2): 66.     CrossRef
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    Sarun Touch, Chalit Komalamisra, Prayong Radomyos, Jitra Waikagul
    Acta Tropica.2009; 111(2): 108.     CrossRef
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    Kim Van Van, Anders Dalsgaard, David Blair, Thanh Hoa Le
    Experimental Parasitology.2009; 123(2): 146.     CrossRef
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    Helle Kay, K. Darwin Murrell, Axel Kornerup Hansen, Henry Madsen, Nguyễn Thi Thu Trang, Nguyen Manh Hung, Anders Dalsgaard
    Journal of Parasitology.2009; 95(3): 629.     CrossRef
  • Fishborne Zoonotic Trematodes in Raw Fish Dishes Served in Restaurants in Nam Dinh Province and Hanoi, Vietnam
    Tran Thi Kim Chi, K.Darwin Murrell, Henry Madsen, Nguyen Viet Khue, Anders Dalsgaard
    Journal of Food Protection.2009; 72(11): 2394.     CrossRef
  • Food-Borne Trematodiases
    Jennifer Keiser, Jürg Utzinger
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  • Prevalence of Zoonotic Trematodes in Fish from a Vietnamese Fish-Farming Community
    Tran T. K. Chi, Anders Dalsgaard, James F. Turnbull, Pham A. Tuan, K. Darwin Murrell
    Journal of Parasitology.2008; 94(2): 423.     CrossRef
  • Prevalence of Zoonotic Metacercariae in Two Species of Grouper, Epinephelus coioides and Epinephelus bleekeri, and Flathead Mullet, Mugil cephalus, in Vietnam
    Dung The Vo, Darwin Murrell, Anders Dalsgaard, Glenn Bristow, Dung Huu Nguyen, Thanh Ngoc Bui, Dung Thi Vo
    The Korean Journal of Parasitology.2008; 46(2): 77.     CrossRef
  • Prevalence of fishborne zoonotic parasites in important cultured fish species in the Mekong Delta, Vietnam
    Pham Cu Thien, Anders Dalsgaard, Bui Ngoc Thanh, Annette Olsen, K. Darwin Murrell
    Parasitology Research.2007; 101(5): 1277.     CrossRef
  • Occurrence and species distribution of fishborne zoonotic trematodes in wastewater‐fed aquaculture in northern Vietnam
    Nguyen Thi Hop, Nguyen Van De, Darwin Murrell, Anders Dalsgaard
    Tropical Medicine & International Health.2007; 12(s2): 66.     CrossRef
  • Fishborne Zoonotic Intestinal Trematodes, Vietnam
    Do Trung Dung, Nguyen Van De, Jitra Waikagul, Anders Dalsgaard, Jong-Yil Chai, Woon-Mok Sohn, K. Darwin Murrell
    Emerging Infectious Diseases.2007; 13(12): 1828.     CrossRef
  • 12,340 View
  • 128 Download
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Case Report

A case of symptomatic splenic infarction in vivax malaria
Areum Kim, Yun-Kyu Park, Jin-Soo Lee, Moon-Hyun Chung, Eun Sil Kim
Korean J Parasitol 2007;45(1):55-58.
Published online March 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.1.55

Splenic infarction is a rare complication in malaria cases, and is caused primarily by Plasmodium falciparum. Recently in South Korea, only P. vivax has prevailed since 1993. Although the probability that symptomatic splenic infarction may occur in vivax malaria cases is considered relatively high, there have never been any case reports describing the occurrence of symptomatic splenic infarction in cases of vivax malaria. A 34-year-old man presented with fever that had persisted for 5 days. P. vivax infection was verified using a peripheral blood smear, and chloroquine was utilized to treat the fever successfully. Six days later, the patient developed pain in the left upper abdomen, which was diagnosed as splenic infarction by computed tomography.

Citations

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  • Splenic Infarction in Plasmodium vivax Infection in South Korea
    Jae Hyoung Im, Moon-Hyun Chung, Areum Durey, Hea Yoon Kwon, Jin-Soo Lee, Man-Jong Lee, Ji Hyeon Baek
    The American Journal of Tropical Medicine and Hygiene.2019; 101(4): 803.     CrossRef
  • Severe Plasmodium vivax infection in Korea
    Jae Hyoung Im, Hea Yoon Kwon, JiHyeon Baek, Seong Wook Park, Areum Durey, Kyung Hee Lee, Moon-Hyun Chung, Jin-Soo Lee
    Malaria Journal.2017;[Epub]     CrossRef
  • Acute necrotizing hemorrhagic pancreatitis in falciparum malaria
    JOSÉ FELIPE REOYO PASCUAL
    Revista Española de Enfermedades Digestivas.2015;[Epub]     CrossRef
  • Imaging of the spleen in malaria
    Mireia Ferrer, Lorena Martin-Jaular, Mariana De Niz, Shahid M. Khan, Chris J. Janse, Maria Calvo, Volker Heussler, Hernando A. del Portillo
    Parasitology International.2014; 63(1): 195.     CrossRef
  • Splenic infarction and malaria
    Francesca F. Norman, Jorge Rojas-Marcos, José-Manuel Hermida-Donate, Begoña Monge-Maillo, Jose Antonio Perez-Molina, Rogelio López-Vélez
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2014; 108(8): 455.     CrossRef
  • Plasmodium vivaxmalaria complicated by splenic infarct
    Varun Aggarwal, Anjali Nagpal, Yatendra Agrawal, Virendra Kumar, Sandeep Kumar Kanwal, Bhavna Dhingra
    Paediatrics and International Child Health.2014; 34(1): 63.     CrossRef
  • Malaria-Induced Splenic Infarction
    Jeong-Hwan Hwang, Chang-Seop Lee
    The American Journal of Tropical Medicine and Hygiene.2014; 91(6): 1094.     CrossRef
  • Massive splenic infarction in children with sickle cell anemia and the role of splenectomy
    Ahmed H. Al-Salem
    Pediatric Surgery International.2013; 29(3): 281.     CrossRef
  • A case of vivax malaria with splenic infarction
    MollyMary Thabah, Mritunjai Kumar, Ananthakrishnan Ramesh, DharanipragadaKrishna Suri Subrahmanyam, Sundararajan Elangovan
    Journal of Vector Borne Diseases.2013; 50(1): 74.     CrossRef
  • The role of the spleen in malaria
    Hernando A. del Portillo, Mireia Ferrer, Thibaut Brugat, Lorena Martin-Jaular, Jean Langhorne, Marcus V. G. Lacerda
    Cellular Microbiology.2012; 14(3): 343.     CrossRef
  • Rare splenic complications and specific serology: decisive diagnostic tools in two cases of visceral leishmaniasis
    Andrea Celestini, Federica Paglia, Orlando Dell’ Unto, Riccardo Guarisco, Claudio Puoti
    Italian Journal of Medicine.2011; 5(4): 274.     CrossRef
  • Population dynamics of a pathogen: the conundrum of vivax malaria
    Philip G. McQueen
    Biophysical Reviews.2010; 2(3): 111.     CrossRef
  • A Case of Myocarditis Associated WithPlasmodium vivaxMalaria
    Soon Ae Kim, Eu Suk Kim, Moo Yong Rhee, Sang Il Choi, Hee Jin Huh, Seok Lae Chae
    Journal of Travel Medicine.2009; 16(2): 138.     CrossRef
  • Pathological rupture of the spleen in malaria: Analysis of 55 cases (1958–2008)
    Patrick Imbert, Christophe Rapp, Pierre A. Buffet
    Travel Medicine and Infectious Disease.2009; 7(3): 147.     CrossRef
  • Resistance to Therapies for Infection byPlasmodium vivax
    J. Kevin Baird
    Clinical Microbiology Reviews.2009; 22(3): 508.     CrossRef
  • Two Cases of Vivax Malaria Accompanied by Splenic Complications (such as splenic rupture and splenic infarction)
    Eun Jung Jung, Eun Ju Choo, Tae Hyong Kim, Min Hyok Jeon, Eun Jeung Lee, Young Sin Cho, Ho Young Lee, Ji Yon Kim
    Infection and Chemotherapy.2008; 40(3): 179.     CrossRef
  • Host Control of Malaria Infections: Constraints on Immune and Erythropoeitic Response Kinetics
    Philip G. McQueen, F. Ellis McKenzie, Rob J. De Boer
    PLoS Computational Biology.2008; 4(8): e1000149.     CrossRef
  • Acute pancreatitis and subdural haematoma in a patient with severe falciparum malaria: Case report and review of literature
    Pratibha Seshadri, Anand Vimal Dev, Surekha Viggeswarpu, Sowmya Sathyendra, John Victor Peter
    Malaria Journal.2008;[Epub]     CrossRef
  • 9,154 View
  • 86 Download
  • Crossref
Brief Communications
Genetic diversity in merozoite surface protein (MSP)-1 and MSP-2 genes of Plasmodium falciparum in a major endemic region of Iran
Aliehsan Heidari, Hossein Keshavarz, Mohammad B Rokni, Tomas Jelinek
Korean J Parasitol 2007;45(1):59-63.
Published online March 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.1.59

Merozoite surface protein-1 (MSP-1) and merozoite surface protein-2 (MSP-2) were used to develop vaccines and to investigate the genetic diversity in Plasmodium falciparum malaria in Iran. Nested polymerase chain reaction amplification was used to determine polymorphisms of block 2 of the MSP-1 and the central domain of MSP-2 genes. A total of 67 microscopically positive P. falciparum infected individuals from a major endemic region, southeast Iran, were included in this trial. Nine alleles of MSP-1 and 11 alleles of MSP-2 were identified. The results showed that amplified product from these surface antigen genes varied in size and there was specific pattern for each isolate. Besides, regarding this pattern, 23 multiple infections with at least 2 alleles were observed. While the endemic regions of malaria in Iran is classified in low to moderate group, but extensive polymorphism was observed for each marker and the MSP-2 central repeat was the most diverse that could be considered in designing malaria vaccine.

Citations

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  • Genetic diversity of Plasmodium falciparum and Plasmodium vivax field isolates from the Nowshera district of Pakistan
    Chandni Hayat, Atif Kamil, Asifullah Khan, Aniqa Sayed, Kehkashan Akbar, Sahib Gul Afridi
    Malaria Journal.2024;[Epub]     CrossRef
  • Population genetic analyses inferred a limited genetic diversity across the pvama-1 DI domain among Plasmodium vivax isolates from Khyber Pakhtunkhwa regions of Pakistan
    Ibrar Ullah, Sahib Gul Afridi, Muhammad Israr, Hizbullah Khan, Sulaiman Shams, Komal Zaib, Huong Giang Le, Jung-Mi Kang, Byoung-Kuk Na, Asifullah Khan
    BMC Infectious Diseases.2022;[Epub]     CrossRef
  • Allelic diversity of MSP1 and MSP2 repeat loci correlate with levels of malaria endemicity in Senegal and Nigerian populations
    Mary A. Oboh, Tolla Ndiaye, Khadim Diongue, Yaye D. Ndiaye, Mouhamad Sy, Awa B. Deme, Mamadou A. Diallo, Mamadou S. Yade, Sarah K. Volkman, Aida S. Badiane, Alfred Amambua-Ngwa, Daouda Ndiaye
    Malaria Journal.2021;[Epub]     CrossRef
  • Prediction of malaria cases in the southeastern Iran using climatic variables
    Hamid Reza Tohidinik, Hossein Keshavarz, Mehdi Mohebali, Mandana Sanjar, Gholamreza Hassanpour
    Asian Pacific Journal of Tropical Medicine.2021; 14(10): 463.     CrossRef
  • Genetic Diversity of Polymorphic Marker Merozoite Surface Protein 1 (Msp-1) and 2 (Msp-2) Genes of Plasmodium falciparum Isolates From Malaria Endemic Region of Pakistan
    Shahid Niaz Khan, Rehman Ali, Sanaullah Khan, Muhammad Rooman, Sadia Norin, Shehzad Zareen, Ijaz Ali, Sultan Ayaz
    Frontiers in Genetics.2021;[Epub]     CrossRef
  • Characterization of drug resistance and genetic diversity of Plasmodium falciparum parasites from Tripura, Northeast India
    S. J. Patgiri, K. Sarma, N. Sarmah, N. Bhattacharyya, D. K. Sarma, T. Nirmolia, D. R. Bhattacharyya, P. K. Mohapatra, D. Bansal, P. K. Bharti, R. Sehgal, J. Mahanta, A. A. Sultan
    Scientific Reports.2019;[Epub]     CrossRef
  • Genetic diversity of merozoite surface protein-2 inPlasmodium falciparumisolates from Aceh province, Indonesia
    K F Jamil, S Supargiyono, D Syafruddin, N Pratama, S Silvy
    IOP Conference Series: Earth and Environmental Science.2018; 125: 012036.     CrossRef
  • The genetic polymorphism of merozoite surface protein-1 inPlasmodium falciparumisolates from Aceh province, Indonesia
    K F Jamil, S Supargiyono, D Syafruddin, N Pratama, S Silvy
    IOP Conference Series: Earth and Environmental Science.2018; 125: 012037.     CrossRef
  • Plasmodium falciparum merozoite surface protein 1 block 2 gene polymorphism in field isolates along the slope of mount Cameroon: a cross – sectional study
    Tobias O. Apinjoh, Rolland B. Tata, Judith K. Anchang-Kimbi, Hanesh F. Chi, Eleanor M. Fon, Regina N. Mugri, Delphine A. Tangoh, Robert V. Nyingchu, Stephen M. Ghogomu, Theresa Nkuo-Akenji, Eric A. Achidi
    BMC Infectious Diseases.2015;[Epub]     CrossRef
  • Genetic polymorphism of merozoite surface protein-1 and merozoite surface protein-2 inPlasmodium falciparumisolates from children in South of Benin
    Aurore Ogouyèmi-Hounto, Dorothée Kinde Gazard, Nicaise Ndam, Elsa Topanou, Olivia Garba, Pancras Elegbe, Tatiana Hountohotegbe, Achille Massougbodji
    Parasite.2013; 20: 37.     CrossRef
  • Genetic diversity of Plasmodium vivax and Plasmodium falciparum in Honduras
    Ana Cecilia Lopez, Andres Ortiz, Jorge Coello, Wilfredo Sosa-Ochoa, Rosa E Mejia Torres, Engels I Banegas, Irina Jovel, Gustavo A Fontecha
    Malaria Journal.2012;[Epub]     CrossRef
  • Genetic diversity of Plasmodium vivax and Plasmodium falciparum in Kohat District, Pakistan
    Lubna Khatoon, Inam Ullah Khan, Shahid Ali Shah, Muhammad Ishtiaq Jan, Farhat Ullah, Salman Akbar Malik
    The Brazilian Journal of Infectious Diseases.2012; 16(2): 184.     CrossRef
  • Reduction of multiplicity of infections but no change in msp2 genetic diversity in Plasmodium falciparum isolates from Congolese children after introduction of artemisinin-combination therapy
    Rod Ibara-Okabande, Felix Koukouikila-Koussounda, Mathieu Ndounga, Jeannhey Vouvoungui, Vladimir Malonga, Prisca Nadine Casimiro, Jean Rosaire Ibara, Anissa Sidibe, Francine Ntoumi
    Malaria Journal.2012;[Epub]     CrossRef
  • Genetic Polymorphism of Plasmodium falciparum Merozoite Surface Protein-1 and -2 and Diversity of Drug Resistance Genes in Blood Donors from Bamako, Mali
    Bolaji N. Thomas, Carlyn R. Petrella, Stephanie R. Crespo, Tanya J. Thakur, Joann M. Moulds, Dapa A. Diallo
    Infectious Diseases: Research and Treatment.2012;[Epub]     CrossRef
  • Genetic polymorphism of merozoite surface protein-1 and merozoite surface protein-2 in Plasmodium falciparum isolates from Brazzaville, Republic of Congo
    Pembe Issamou Mayengue, Mathieu Ndounga, Freddy Vladimir Malonga, Michel Bitemo, Francine Ntoumi
    Malaria Journal.2011;[Epub]     CrossRef
  • Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum
    Eva M. Moyano, Luis Miguel González, Laureano Cuevas, Esperanza Perez-Pastrana, Ysmael Santa-Maria, Agustín Benito
    Molecular Biology Reports.2010; 37(6): 2893.     CrossRef
  • Genetic diversity among Plasmodium falciparum field isolates in Pakistan measured with PCR genotyping of the merozoite surface protein 1 and 2
    Najia K Ghanchi, Andreas Mårtensson, Johan Ursing, Sana Jafri, Sándor Bereczky, Rabia Hussain, Mohammad A Beg
    Malaria Journal.2010;[Epub]     CrossRef
  • Genetic structure of Plasmodium vivax and Plasmodium falciparum in the Bannu district of Pakistan
    Lubna Khatoon, Frederick N Baliraine, Mariangela Bonizzoni, Salman A Malik, Guiyun Yan
    Malaria Journal.2010;[Epub]     CrossRef
  • Genetic polymorphism of merozoite surface protein-1 and merozoite surface protein-2 in Plasmodium falciparum field isolates from Myanmar
    Jung-Mi Kang, Sung-Ung Moon, Jung-Yeon Kim, Shin-Hyeong Cho, Khin Lin, Woon-Mok Sohn, Tong-Soo Kim, Byoung-Kuk Na
    Malaria Journal.2010;[Epub]     CrossRef
  • Dynamics of Plasmodium falciparum alleles in children with normal haemoglobin and with sickle cell trait in western Uganda
    Gertrude N. Kiwanuka, Hema Joshi, William K. Isharaza, Klaus Eschrich
    Transactions of the Royal Society of Tropical Medicine and Hygiene.2009; 103(1): 87.     CrossRef
  • 8,258 View
  • 89 Download
  • Crossref
Application of Giemsa stain for easy detection of Trichinella spiralis muscle larvae
Carmen Ram?rez-Melgar, Alberto G?mez-Priego, Jorge-Luis De-La-Rosa
Korean J Parasitol 2007;45(1):65-68.
Published online March 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.1.65

The application of Giemsa technique to stain compressed diaphragm samples obtained from rodents experimentally infected with Trichinella spiralis is described. Diaphragm samples from rats heavily infected with 20 muscle larvae per gram of body weight (20 ML/gbw) were cut into several pieces and stained with Giemsa; on the other hand, whole diaphragms from slightly infected mice (1 ML/gbw) were also stained with Giemsa. Besides, muscle samples were also stained with Giemsa. Observation at 10 × magnification revealed that both ML and nurse cells (NC) look as bluish structures clearly contrasting with the pinkish color of the non-infected muscle fibers. NC in the diaphragms of mice could be easily observed at naked eye as blue points contrasting with the pink surrounding areas formed by the non-infected muscle fibers. Among NC observed in the diaphragms of rats infected with 20 ML/gbw, 4.4% was multiple infection. These findings were confirmed in sectioned and hematoxylin-eosin stained specimens. This data could be usefulness for a rapid diagnosis of trichinellosis in post-mortem mammals without magnification procedures.

Citations

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  • Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of Trichinella spiralis in Experimentally Infected Mice
    Vicente Vega-Sánchez, Fabián-Ricardo Gómez-De-Anda, Georgina Calderón-Domínguez, Mary-Carmen-del-Sol Ramírez-y-Ramírez, Nydia-E. Reyes-Rodríguez, Andrea-P. Zepeda-Velázquez, Raquel Tapia-Romero, Jorge-Luis de-la-Rosa-Arana
    Pathogens.2021; 10(11): 1382.     CrossRef
  • Immune response to aTrichinella spiralisinfection in house mice from lines selectively bred for high voluntary wheel running
    Elizabeth M. Dlugosz, Heidi Schutz, Thomas H. Meek, Wendy Acosta, Cynthia J. Downs, Edward G. Platzer, Mark A. Chappell, Theodore Garland
    Journal of Experimental Biology.2013;[Epub]     CrossRef
  • Comparative effects of levamisole, Staphylococcus, and Freund's adjuvant on rat immunization with excretory and secretory antigens of Trichinella spiralis muscle larvae
    Jorge-Luis de-la-Rosa-Arana, Rafael Campos-Rodríguez, Víctor Rivera-Aguilar, Alejandro Escobar-Gutiérrez, Ángel Miliar-García, Norma-Elena Herrera-González, Rosa-Adriana Jarillo-Luna
    Parasitology Research.2012; 111(4): 1599.     CrossRef
  • Influence of different processing procedures on the reproductive capacity of Trichinella spiralis in pork meat
    M. S. Medina-Lerena, A. Ramirez-Álvarez, M. Kühne, A. Gómez-Priego, J.-L. de-la-Rosa
    Tropical Animal Health and Production.2009; 41(4): 437.     CrossRef
  • 8,809 View
  • 119 Download
  • Crossref
Differential diagnosis of Trichostrongylus and hookworm eggs via PCR using ITS-1 sequence
Tai-Soon Yong, Jong-Ho Lee, Seobo Sim, Jongweon Lee, Duk-Young Min, Jong-Yil Chai, Keeseon S. Eom, Woon-Mok Sohn, Soon-Hyung Lee, Han-Jong Rim
Korean J Parasitol 2007;45(1):69-74.
Published online March 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.1.69

Trichostrongylus eggs observed in cellophane-thick smears are difficult, in practice, to distinguish from hookworm eggs. In order to overcome these limitations, a molecular approach was conducted. A Trichostrongylus colubriformis adult worm was obtained from a human in Laos, which was identified morphologically. ITS-1 sequence of this worm was determined, and found to be most similar with that of T. colubriformis among the Trichostrongylus spp. reported so far. Then, this sequence was compared with those of human hookworm species, Ancylostoma duodenale and Necator americanus, and species-specific oligonucleotide primers were designed. Polymerase chain reaction (PCR) using these primers evidenced specifically amplified PCR products of Trichostrongylus sp., A. duodenale and N. americanus from the eggs of each (520 bp, 690 bp, and 870 bp, respectively). A species-specific PCR technique can be developed in order to study the epidemiology of Trichostrongylus spp. and hookworms in endemic areas.

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  • Molecular Characterization and Analysis of Human Trichostrongylus Species in an Endemic Region of Iran Based on COX 1 Gene; A Cross‐Sectional Study
    Sara Nemati, Hanieh Mohammad Rahimi, Meysam Sharifdini, Hamed Mirjalali
    Health Science Reports.2025;[Epub]     CrossRef
  • Trichostrongylosis: a zoonotic disease of small ruminants
    A.H. Bhat, H. Tak, I.M. Malik, B.A. Ganai, N. Zehbi
    Journal of Helminthology.2023;[Epub]     CrossRef
  • Performance of microscopy compared to conventional PCR in identification of soil-transmitted helminth infections among antenatal women in a low-prevalence setting
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    Indian Journal of Medical Microbiology.2023; 46: 100427.     CrossRef
  • Prevalence and risk factors associated with gastrointestinal parasites in goats (Capra hircus) and sheep (Ovis aries) from three provinces of China
    Weimin Cai, Cheng Cheng, Qianqian Feng, Yifei Ma, Enyu Hua, Shimin Jiang, Zhaofeng Hou, Dandan Liu, Anlong Yang, Darong Cheng, Jinjun Xu, Jianping Tao
    Frontiers in Microbiology.2023;[Epub]     CrossRef
  • Prevalence of human trichostrongyliasis in Iran: a systematic review and meta-analysis
    Bahman Rahimi-Esboei, Maryam Pourhajibagher, Abbas Bahador
    Reviews in Medical Microbiology.2022; 33(1): e16.     CrossRef
  • Morphologic Characterization of Strongylida Larvae from Human and Swine Coprocultures in Rural Communities in the State of Piauí, Northeastern Brazil
    Polyanna A. A. Bacelar, Kerla J. L. Monteiro, Jéssica P. dos Santos, Denilson de A. e Silva, Daniella N. Leal, Mayron M. Almeida, Brenda B. C. Evangelista, Francisco M. de Oliveira-Neto, Filipe A. Carvalho-Costa, Pedro P. Chieffi
    Journal of Tropical Medicine.2022; 2022: 1.     CrossRef
  • Assessing the efficacy of albendazole against hookworm in Vietnam using quantitative PCR and sodium nitrate flotation
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