Cercariae of Clonorchis sinensis were attempted to penetrate into various fry of fresh water fishes in order to examine the conditions of infection and the further development in the fish. The following conclusions were made. Cercariae of C. sinensis penetrated into the muscle of the fry of Pseudorasbora parva were found to be able to develop.
Cercariae penetrated into the fry of Pseudorasbora parva migrated into the organs of the fry to kill their host. From each dead individual of P. parva, more than 10 larvae of C.
sinensis could be discovered. Cercariae attached on the Cyprinus carpio, Carassius and gold fish were almost killed before penetration into the body and even one or two cercariae penetrated were also killed as time passed.
Cercariae of C. sinensis infected on the fry of Cyprinus Carpio, Carassius auratus and gold fish could not survive more than 60 days. Cercariae penetrated into Cyprinus carpio and Carassius auratus could not develop into complete metacercariae even after 3O days. Cercariae of Clonorchis sinensis penetrated into the fry of Aplocheilus latipes were killed within 10 hours and the cercariae attached on the caudal fin were destroyed in 2 hours. It was found that no cercariae of Clonorchis sinensis could penetrate into the body of the fry of Misgurnus anguillicaudatus.
Citations
Citations to this article as recorded by
Comparison of infectivity, metacercarial burden and host mortality induced byOpisthorchis viverrinisensu lato cercariae from Lao PDR compared with Thailand in cyprinid fish,Barbonymus gonionotus Kulthida Kopolrat, Paiboon Sithithaworn, Nadda Kiatsopit, Opal Pitaksakulrat, Smarn Tesana, Ross H. Andrews, Trevor N. Petney Transactions of The Royal Society of Tropical Medicine and Hygiene.2016; 110(1): 46. CrossRef
Susceptibility, metacercarial burden, and mortality of juvenile silver barb, common carp, mrigal, and tilapia following exposure to Haplorchis taichui Kulthida Kopolrat, Paiboon Sithithaworn, Smarn Tesana, Ross H. Andrews, Trevor N. Petney Parasitology Research.2015; 114(4): 1433. CrossRef
The wormicidal substances of fresh water fishes on Clonorchis sinensis VII. The effect of linoleic acid and ethyl linoleate on parasite viability Jae Ku Rhee, Sang Bork Lee, Pyung Gil Kim The Korean Journal of Parasitology.1988; 26(3): 175. CrossRef
Experimental studies on the second intermediate hosts of Clonorchis sinensis IV. Observations on the fate of metacercariae of Clonorchis sinensis in the fish host, Cultriculus eigenmanni Jae Ku Rhee, Byeong Kirl Baek, Ho Il Lee The Korean Journal of Parasitology.1985; 23(1): 79. CrossRef
The wormicidal substances of fresh water fishes on Clonorchis sinensis I Preliminary research on the wormicidal substance from mucous substance of Carassius carassius Jae Ku Rhee, Byeong Kirl Baek, Byung Zun Ahn, Young Jun Park The Korean Journal of Parasitology.1979; 17(2): 121. CrossRef
Experimental studies on the second intermediate hosts of Clonorchis sinensis. III. Observations on the relationship between clavate cells of epidermis and infectivity of metacercariae of Clonorchis sinensis in fresh-water fish Jae Ku Rhee The Korean Journal of Parasitology.1974; 12(2): 101. CrossRef
Studies on the experimental mode of infections of Clonorchis sinensis. III. Studies on the wormicidal effect of external mucous substance of some fresh water fish on the larva of Clonorchis sinensis Seh Kyu Chun The Korean Journal of Parasitology.1964; 2(3): 148. CrossRef
The author reported in the previous pager on his observation of sudden death of Clonorchis sinensis cercaria before penetration into the body of fry such as Cyprinus carpio and Carassius auratus. And in these studies, the reason behind the fact was investigated and it was found that the mucous substances on the surface of a certain fishes have a strong wormicidal effect to the larva of C. sinensis. When cercariae of C. sinensis were immersed into the mucous substance of gold fish for three minutes 36 seconds to 4 minutes 20 seconds, into that of Aplocheilus latipes for 4 minutes 30 seconds to 5 minutes 20 seconds, into that of Cyprinus carpio for 5 minutes 15 seconds to 6 minutes l6 seconds, and into that of Carassius auratus for 5 minutes 20 seconds to 7 minutes 30 seconds, it was observed that they were all killed. No wormicidal effect against C. sinensis could be observed with the mucous substance obtained from Pseudorasbora parva, Misgurnus anguillicaudatus and Ophicephalus argus. No wormicidal effect against Metagonimus sp. cercaria is observed with the mucous substance obtained from Cyprinus carpio, Carassius auratus. The wormicidal effect of the mucous substances obtained from gold fish was destroyed when heated over 60 C. The movements of metacercaria of C. sinensis stopped after 10 minutes application of the mucous substance obtained from Cyprinus carpio and they were killed within 30 minutes. When the adult worms collected from the bile duct of experimentally infected rabbits for 20 to 60 days, were immersed into mucous substance from Cyprinus carpio, they were killed between 25 minutes and 35 minutes. No wormicidal effect could be observed with the mucous substance obtained from dead Cyprinus carpio, Carassius auratus, gold fish, when applied to the adult worms and larvae of C. sinensis.
Citations
Citations to this article as recorded by
The wormicidal substances of fresh water fishes on Clonorchis sinensis VII. The effect of linoleic acid and ethyl linoleate on parasite viability Jae Ku Rhee, Sang Bork Lee, Pyung Gil Kim The Korean Journal of Parasitology.1988; 26(3): 175. CrossRef
Larvicidal action of liquid nitrogen against metacercariae of Clonorchis sinensis Soo Bok Song The Korean Journal of Parasitology.1987; 25(2): 123. CrossRef
Experimental studies on the second intermediate hosts of Clonorchis sinensis IV. Observations on the fate of metacercariae of Clonorchis sinensis in the fish host, Cultriculus eigenmanni Jae Ku Rhee, Byeong Kirl Baek, Ho Il Lee The Korean Journal of Parasitology.1985; 23(1): 79. CrossRef
The wormicidal substances of fresh water fishes on Clonorchis sinensis I Preliminary research on the wormicidal substance from mucous substance of Carassius carassius Jae Ku Rhee, Byeong Kirl Baek, Byung Zun Ahn, Young Jun Park The Korean Journal of Parasitology.1979; 17(2): 121. CrossRef
Experimental studies on the second intermediate hosts of Clonorchis sinensis. III. Observations on the relationship between clavate cells of epidermis and infectivity of metacercariae of Clonorchis sinensis in fresh-water fish Jae Ku Rhee The Korean Journal of Parasitology.1974; 12(2): 101. CrossRef
A comparison of the absorption and incorporation of C(14)-proline into protein by the 7 kinds of helminth parasites is presented. The radioactivity of free amino acid fraction is greater than that of protein fraction in all the worms, and only a small amount of exogenous labeled proline is incorporated into tissue protein. In general, the pattern of C(14)-proline uptake and its incorporation into protein shows rapid linear increase during the period of 15 to 30 min and reaches the maximum at 60 min after incubation, and then the equilibrium state was maintained throughout further incubation.
Citations
Citations to this article as recorded by
Autoradiographic studies on the uptake of C(14)-succinic acid by Clonorchis sinensis Seung Soo Han The Korean Journal of Parasitology.1971; 9(1): 17. CrossRef
Metabolism of C(14)-glycine by Clonorchis sinensis Soo Hyun Seong, Byong Seol Seo The Korean Journal of Parasitology.1966; 4(2): 14. CrossRef
Studies on transaminase reactions in some parasitic helminths Yong Ok Min, Byong Seol Seo The Korean Journal of Parasitology.1966; 4(2): 7. CrossRef
Autoradiographic studies on some parasitic helminth Joo Soo Yoon, Soon Hyung Lee, Byong Seol Seo The Korean Journal of Parasitology.1964; 2(3): 165. CrossRef
Direct contact method of autoradiography was utilized in studying the distribution of exogenous C(14)-proline in Clonorchis sinensis, Eurytrema pancreaticum, Hymenolepis diminuta and Dipylidium caninum. The most distinct autoradiogram were obtained after 60 min of incubation, corresponding to the maximal absorption of C(14)-proline in these parasite. The radioactivitity of this labeled amino acid was chiefly concentrated in reproductive organs, especially egg-containing uterine tubules.
Citations
Citations to this article as recorded by
Autoradiographic study on the uptake of (3)H-6-thymidine by Fasciola hepatica Joong Ho Kim The Korean Journal of Parasitology.1973; 11(1): 13. CrossRef
Autoradiographic studies on the uptake of C(14)-succinic acid by Clonorchis sinensis Seung Soo Han The Korean Journal of Parasitology.1971; 9(1): 17. CrossRef
Metabolism of C(14)-glycine by Clonorchis sinensis Soo Hyun Seong, Byong Seol Seo The Korean Journal of Parasitology.1966; 4(2): 14. CrossRef
Studies on the metabolism of C(14)-proline in some parasitic helminths Joo Soo Yoon The Korean Journal of Parasitology.1964; 2(3): 159. CrossRef
The glucose uptake rate by Fasciola hepatica was a mean value of 9.62 +/- 0.54 micro-mole/hr/g, and total CO(2) production rate by the flukes averaged 24.28 +/- 4.26 micro-mole/hr/g wet wt. The relative specific activity of respiratory CO(2) showed a mean value of 79.89 +/- 1.78 per cent. The rate of CO(2) production derived from medium C(14)-glucose was a mean of 19.55 +/- 3.56 micro-mole/hr/g of we wt. Therefore, the average value of 32.72 +/- 4.8 percent of glucose utilized by the flukes from the medium C(14)-glucose was oxidized to respiratory CO(2). The tissue concentration of glycogen in F. hepatica was a mean of 38.36 +/- 2.91 mg/g or 3.84 +/- 0.29 %/g of wet wt, and the turnover rate of glycogen pool was a mean of 1.6+/-0.22 %/hr or 0.65 +/- 0.13 mg/hr/g. The average value of 37.26 +/- 3.86 per cent of glucose utilized by the fluke from the medium C(4)-glucose was incorporated to the glycogen. These data account for that approximately 70 per cent of the utilized glucose by the flukes participated in furnishing the oxidation into respiratory CO(2) and the synthetic process into glycogen.
Citations
Citations to this article as recorded by
Autoradiographic study on the uptake of (3)H-6-thymidine by Fasciola hepatica Joong Ho Kim The Korean Journal of Parasitology.1973; 11(1): 13. CrossRef
Study on the (14)C-glucose metabolism by Clonorchis sinensis Il Kwon Kang, Soon Hyung Lee, Byong Seol Seo The Korean Journal of Parasitology.1969; 7(3): 143. CrossRef
Metabolism of C(14)-glycine by Clonorchis sinensis Soo Hyun Seong, Byong Seol Seo The Korean Journal of Parasitology.1966; 4(2): 14. CrossRef
Metabolism of C(14)-acetate by cestodes Han Jong Rim, Chung Jai Park, Yong Ok Min, Byong Jong On, Hyun Kyo Lee, Myong Soon Yun The Korean Journal of Parasitology.1965; 3(3): 122. CrossRef
Metabolism of C(14)-glucose by Ascaridia galli Han Jong Rim, Kwang Soo Kim, Soo Hyun Seong, Sang Don Rhee, Byong Jong On, Hyun Kyo Lee The Korean Journal of Parasitology.1965; 3(3): 107. CrossRef
Metabolism of C(14)-acetate by some trematodes Byong Seol Seo, Han Jong Rim, Yong Ok Min, Sang Don Rhee, Tong Hoon Lee, Myong Soon Yun The Korean Journal of Parasitology.1965; 3(3): 117. CrossRef
Metabolism of C(14)-glucose by Moniezia expansa and Diphyllobothrium mansoni Han Jong Rim, Soo Hyun Seong, Chung Jai Park, Sang Don Rhee, Byong Jong Oh, Hyu Kyo Lee The Korean Journal of Parasitology.1965; 3(3): 112. CrossRef
Metabolism of C(14)-glucose by Eurytrema pancreaticum Byong Seol Seo, Han Jong Rim, Kwang Soo Kim, Myung Sang Lee, Yeong Uhn Kim, Hi Yong Song The Korean Journal of Parasitology.1964; 2(3): 175. CrossRef
The glucose uptake rate by Eurytrema pancreaticum was a mean value of 16.44 +/- 2.42 micro-mole/hr/g, and total CO(2) production rate by the fluke averaged 5.82 +/- 0.97 micro-mole/hr/g. The relative specific activity of respiratory CO(2) showed a mean value of 5.75 +/- 0.84 per cent. The rate of CO(2) production derived from medium C(14)-glucose was a mean of 0.33 +/- 0.10 micor-mole/hr/g.
Therefore, the average value of 0.32 +/- 0.04 per cent of glucose utilized by the flukes from the medium C(14)-glucose was oxidized to respiratory CO(2). The tissue concentration of glycogen in E. pancreaticum was a mean of 45.50 +/- 2.18 mg/g or 4.55 +/- 0.22 %/g. But the turnover rate of glycogen pool was a mean of 0.027 +/- 0.003 %/hr or 0.009 +/- 0.002 mg/hr/g. The average value of 0.64 +/- 0.23 percent of glucose utilized by the flukes from the medium C(14)-glucose was incorporated into the glycogen. These data account for that only 1 per cent of the utilized glucose by the flukes participated in furnishing the oxidation into respiratory CO(2) and the synthetic process into glycogen.
Citations
Citations to this article as recorded by
Study on the (14)C-glucose metabolism by Clonorchis sinensis Il Kwon Kang, Soon Hyung Lee, Byong Seol Seo The Korean Journal of Parasitology.1969; 7(3): 143. CrossRef
Metabolism of C(14)-glycine by Clonorchis sinensis Soo Hyun Seong, Byong Seol Seo The Korean Journal of Parasitology.1966; 4(2): 14. CrossRef
Metabolism of C(14)-acetate by cestodes Han Jong Rim, Chung Jai Park, Yong Ok Min, Byong Jong On, Hyun Kyo Lee, Myong Soon Yun The Korean Journal of Parasitology.1965; 3(3): 122. CrossRef
Metabolism of C(14)-glucose by Moniezia expansa and Diphyllobothrium mansoni Han Jong Rim, Soo Hyun Seong, Chung Jai Park, Sang Don Rhee, Byong Jong Oh, Hyu Kyo Lee The Korean Journal of Parasitology.1965; 3(3): 112. CrossRef
Metabolism of C(14)-acetate by some trematodes Byong Seol Seo, Han Jong Rim, Yong Ok Min, Sang Don Rhee, Tong Hoon Lee, Myong Soon Yun The Korean Journal of Parasitology.1965; 3(3): 117. CrossRef
Metabolism of C(14)-glucose by Ascaridia galli Han Jong Rim, Kwang Soo Kim, Soo Hyun Seong, Sang Don Rhee, Byong Jong On, Hyun Kyo Lee The Korean Journal of Parasitology.1965; 3(3): 107. CrossRef
A case of sparganosis was presented. The patient was a 43-year-old Korean male who had a history of having eaten raw flesh of snake and frog in Keoje Island during 1950 to 1952 for the purpose of treatment of arthritis by the oriental custom. In the summer, l959, he had slight itching sensation in the left lower scrotal area. A few months later, he noticed the appearance of a peanut-sized mass in that area. However, the mass enlarged gradually to a hen's egg size during two years. On surgical exploration, the mass was found to be a fibro-connective tissue capsule(3 to 4 cm in diameter) in the lower left scrotal area. From the capsule three larvae(l9 to 28 cm by 2 to 6 mm) were freed by dissection without much difficulty. The sources of infection of some formerly reported sparganosis in Korea were discussed. It is most probable that human sparganosis is acquired by ingestion of raw flesh of snake and frog in this country.
Citations
Citations to this article as recorded by
A Retrieved Sparganum of Spirometra erinaceieuropaei from a Korean Man during Mechanical Thrombectomy Yang-Ha Hwang, Wonsoo Son, Yong-Won Kim, Dong-Hun Kang, Hyun-Ha Chang, Youn-Kyoung Goo, Yeonchul Hong, Dong-Il Chung The Korean Journal of Parasitology.2020; 58(3): 309. CrossRef
Scrotal Sparganosis Mimicking Scrotal Teratoma in an Infant: A Case Report and Literature Review Yi-Ming Zhao, Hao-Chuan Zhang, Zhong-Rong Li, Hai-Yan Zhang The Korean Journal of Parasitology.2014; 52(5): 545. CrossRef
Sparganosis in Russell’s viper snake: a case report K. T. Kavitha, B. R. Latha, S. T. Bino Sundar, R. Sridhar, S. Abdul Basith Journal of Parasitic Diseases.2014; 38(4): 394. CrossRef
Apoptogenic activity and toxicity studies of a cytotoxic protein (BMP1) from the aqueous extract of common Indian toad (Bufo melanostictus Schneider) skin Pushpak Bhattacharjee, Biplab Giri, Antony Gomes Toxicon.2011; 57(2): 225. CrossRef
A surgically confirmed case of breast sparganosis showing characteristic mammography and ultrasonography findings Jae-Hwan Park, Jee-Won Chai, Nariya Cho, Nam-Sun Paek, Sang-Mee Guk, Eun-Hee Shin, Jong-Yil Chai The Korean Journal of Parasitology.2006; 44(2): 151. CrossRef
Cestode infections in Korea D Y Min The Korean Journal of Parasitology.1990; 28(Suppl): 123. CrossRef
Some aspects of human sparganosis in Korea Seung Yull Cho, Jong Hoa Bae, Byong Seol Seo The Korean Journal of Parasitology.1975; 13(1): 60. CrossRef
On the Sparganum mansoni infection in some Korean terrestrial snakes Seung Yull Cho, Koo Il Hwang, Byong Seol Seo The Korean Journal of Parasitology.1973; 11(2): 87. CrossRef
The question of infectivity and prevalence of Clonorchis sinensis in Cheju province (Quelpart Island) was arisen to authors since the several surveys on the endemic diseases were performed in this island. Therefore, authors decided to solute this question. Then the survey on the possible second intermediate host of Clonorchis sinensis was performed and reported already with negative finding. At present time, authors carried out the collection of all kinds of fresh-water snails through all areas of this island, to confirm the presence or not of Parafossarulus manchouricus, the only first intermediate host of Clonorchis sinensis in Korea . And the following results were obtained. The fresh-water snails collected in the survey were Semisulcospira libertina Gould and Lymnaea ollula Gould.
Parafossarulus manchouricus Bourguigant was not collected in this island. It is confirmed that the Clonorchis sinensis can not be prevalent in this island.
Citations
Citations to this article as recorded by
An evaluation of intraderaml tests for the screening of paragonimiasis by comparing antigens variously processed Joung Soon Kim The Korean Journal of Parasitology.1969; 7(1): 15. CrossRef
A study on the infection status on intermediate hosts by Paragonimus on Che Ju Island Joung Soon Kim The Korean Journal of Parasitology.1969; 7(3): 171. CrossRef
Authors carried out the survey for human paragonimiasis in Ko-Hoong County, Chun-Nam Province, Korea from July to September 1964 by the use of intradermal screening test with Veronal buffered saline antigen. Eight myuns (village) out of 13 myuns of this county were selected for the survey, regarding balanced selection. The number of 2,916 primary school children of high class and 764 residents of all age groups in these areas were examined (total 3,680; male 1,961; female 1,719). The following results were obtained in this survey. As a whole, 20.1 per cent of the examiners showed positive reaction, with sexual difference of 20.9 per cent in male and 19.1 per cent in female. In regional differences of positive rate, the highest rate(50.1 per cent) was showed at Po-Doo myun and lowest rate(3.6 per cent) was showed at Do-Yang myun. In age group, the highest positive rate was showed in 16 to 20 age group (46.7 per cent). In regional comparison of paragonimiasis infectivity of whole nation (province and county), Ko-Hoong County as nearly same as the Hea-Nam County, Chun-Nam Province follows next of Cheju Province, the highest prevalance area of paragonimiasis in Korea, in concentration of prevalence of paragonimiasis.
Citations
Citations to this article as recorded by
Paragonimus and paragonimiasis in Korea D W Choi The Korean Journal of Parasitology.1990; 28(Suppl): 79. CrossRef
Epidemiological survey on paragonimiasis in Kang Hwa Gun Han Jong Rim, Joon Sang Lee, Han Sung Chung, Il Hyun, Kye Hun Jung The Korean Journal of Parasitology.1975; 13(2): 139. CrossRef
First
Prev
