Three morphological types of the genus Metagonimus were found encysted in the dace, Tribolodon taczanowskii, caught from the Sumjin River, Korea. They include Metagonimus Yokogawa type (M. yokogawai), Miyata type, and Koga type according to Saito's classification. The metacercariae were experimentally fed to rats and hamsters and a total of 1,624 Metagonimus worms were recovered from their small intestine.
The number of each type was 820 for Yokogawa type, 688 for Miyata type, 46 for Koga type, and 70 undetermined. The major differential keys between Yokogawa and Miyata types were in the position of two testes, distribution of uterine tubules, and size of the eggs, and the keys between Miyata and Koga types were in the position of two testes, distribution of vitelline follicles, and size of the eggs.
The validity of Miyata type as a specific level should be retained until more convincing evidences are obtained. Koga type is regarded as a synonym of M. takahashii. The present results reveal that there are 3 morphological types of Metagonimus whose larvae are encysted in the dace, T.
taczanowskii, from the Sumjin River.
Citations
Citations to this article as recorded by
Infection characteristics of Metagonimus species (Digenea: Heterophyidae) metacercariae in fish from major rivers of Korea Woon-Mok Sohn Parasites, Hosts and Diseases.2024; 62(1): 1. CrossRef
Metagonimus yokogawai Ancient DNA Recovered from 16th- to 17th-Century Korean Mummy Feces of the Joseon Dynasty Jong Ha Hong, Min Seo, Chang Seok Oh, Jong-Yil Chai, Dong Hoon Shin Journal of Parasitology.2020;[Epub] CrossRef
Infection Status with Metagonimus spp. Metacercariae in Fishes from Seomjin-gang and Tamjin-gang in Republic of Korea Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Jung-Won Ju, Cheon-Hyeon Kim, Ki-Bok Yoon The Korean Journal of Parasitology.2018; 56(4): 351. CrossRef
Fishborne zoonotic heterophyid infections: An update Jong-Yil Chai, Bong-Kwang Jung Food and Waterborne Parasitology.2017; 8-9: 33. CrossRef
Metacercaria Infection Status of Fishborne Zoonotic Trematodes, Except for Clonorchis sinensis in Fish from the Heilongjiang Province, China Jian-Hua Qiu, Ying Zhang, Xiao-Xuan Zhang, Yuan Gao, Qi Li, Qiao-Cheng Chang, Chun-Ren Wang Foodborne Pathogens and Disease.2017; 14(8): 440. CrossRef
A molecular phylogeny of Asian species of the genus Metagonimus (Digenea)—small intestinal flukes—based on representative Japanese populations Siritavee Pornruseetairatn, Hideto Kino, Takeshi Shimazu, Yukifumi Nawa, Tomáš Scholz, Jiraporn Ruangsittichai, Naowarat Tanomsing Saralamba, Urusa Thaenkham Parasitology Research.2016; 115(3): 1123. CrossRef
Heterophyid trematodes recovered from people residing along the Boseong River, South Korea Jong-Yil Chai, Bong-Kwang Jung, Deok-Gyu Kim, Jae-Lip Kim, Hyemi Lim, Eun-Hee Shin, Keon Hoon Lee, Mok Ryeon Kim, Sun Jin Han, Jae Hyun Yeom, Sun Mi Park, Jae-Sun Hwang Acta Tropica.2015; 148: 142. CrossRef
Prevalence of Zoonotic Trematode Metacercariae in Freshwater Fish from Gangwon-do, Korea Shin-Hyeong Cho, Won-Ja Lee, Tong-Soo Kim, Won-Seok Seok, Taejoon Lee, Kyungjin Jeong, Byoung-Kuk Na, Woon-Mok Sohn The Korean Journal of Parasitology.2014; 52(4): 399. CrossRef
Metagonimus yokogawai: metacercariae survey in fishes and its development to adult worms in various rodents Ming-Hsien Li, Hai-I Huang, Pei-Lain Chen, Chiung-Hua Huang, Yu-Hsuan Chen, Hong-Kean Ooi Parasitology Research.2013; 112(4): 1647. CrossRef
Foodborne Intestinal Flukes in Southeast Asia Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim The Korean Journal of Parasitology.2009; 47(Suppl): S69. CrossRef
Fish-borne Zoonotic Trematode Metacercariae in the Republic of Korea Woon-Mok Sohn The Korean Journal of Parasitology.2009; 47(Suppl): S103. CrossRef
Fishborne Trematode Metacercariae in Freshwater Fish from Guangxi Zhuang Autonomous Region, China Woon-Mok Sohn, Keeseon S. Eom, Duk-Young Min, Han-Jong Rim, Eui-Hyug Hoang, Yichao Yang, Xueming Li The Korean Journal of Parasitology.2009; 47(3): 249. CrossRef
Food-borne intestinal trematode infections in the Republic of Korea Jong-Yil Chai, Soon-Hyung Lee Parasitology International.2002; 51(2): 129. CrossRef
Field investigations on epidemiology and control of fish-borne parasites in Korea Han-Jong Rim International Journal of Food Science and Technology.1998; 33(2): 157. CrossRef
Different RAPD patterns between Metagonimus yokogawai and Metagonimus Miyata type J R Yu, J S Chung, J Y Chai The Korean Journal of Parasitology.1997; 35(4): 295. CrossRef
Metagonimus miyatai sp. nov. (Digenea: Heterophyidae), a new intestinal trematode transmitted by freshwater fishes in Japan and Korea S Saito, J Y Chai, K H Kim, S H Lee, H J Rim The Korean Journal of Parasitology.1997; 35(4): 223. CrossRef
Classification and host specificity of Metagonimus spp. from Korean freshwater fish H J Rim, K H Kim, K H Joo The Korean Journal of Parasitology.1996; 34(1): 7. CrossRef
Intestinal flukes of genus Metagonimus and their second intermediate hosts in Kangwon-do Y K Ahn The Korean Journal of Parasitology.1993; 31(4): 331. CrossRef
An epidemiological study of metagonimiasis along the upper reaches of the Namhan River J Y Chai, S Huh, J R Yu, J Kook, K C Jung, E C Park, W M Sohn, S T Hong, S H Lee The Korean Journal of Parasitology.1993; 31(2): 99. CrossRef
We surveyed 12 migrating large egrets, Egretta alba modesta, for their infection status with intestinal trematodes, from June to September, 1990. All of them were infected with one or more flukes. Total 5 species were identified. Their infection rates and total numbers of worms were Metagonimus sp. 67% and 1,412, Centrocestus armatus 67% and 2,306, Echinochasmus japonicus 50% and 99, Strigea falconis 83% and 635, and a diplostomid fluke 33% and 36.
The number of worms in a bird was in the range from 6 to 896. This is the first record that the egret is a reservoir host of Metagonimus sp., C. armatus, E. japonicus, and S.
falconis. S. falconis is a new parasite fauna in Korea.
Migrating birds must play an important role in the transmission and spreading of these intestinal trematodes in Korea.
Citations
Citations to this article as recorded by
First species record of Strigea falconis Szidat, 1928 (Trematoda, Strigeidae) from gyrfalcon Falco rusticolus in Iceland—pros and cons of a complex life cycle Anna Faltýnková, Damien Jouet, Ólafur Karl Nielsen, Karl Skírnisson Parasitology Research.2024;[Epub] CrossRef
Infections with Digenetic Trematode Metacercariae in Freshwater Fishes from Two Visiting Sites of Migratory Birds in Gyeongsangnam-do, Republic of Korea Woon-Mok Sohn, Byoung-Kuk Na The Korean Journal of Parasitology.2019; 57(3): 273. CrossRef
Infections with Centrocestus armatus Metacercariae in Fishes from Water Systems of Major Rivers in Republic of Korea Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Jung-Won Ju, Cheon-Hyeon Kim, Ki-Bok Yoon, Jai-Dong Kim, Dong Cheol Son, Soon-Won Lee The Korean Journal of Parasitology.2018; 56(4): 341. CrossRef
Fishborne zoonotic heterophyid infections: An update Jong-Yil Chai, Bong-Kwang Jung Food and Waterborne Parasitology.2017; 8-9: 33. CrossRef
Three Echinostome Species from Wild Birds in the Republic of Korea Seongjun Choe, Dongmin Lee, Hansol Park, Mihyeon Oh, Hyeong-Kyu Jeon, Youngsun Lee, Ki-Jeong Na, Youngjun Kim, Hang Lee, Keeseon S. Eom The Korean Journal of Parasitology.2014; 52(5): 513. CrossRef
Import of exotic and zoonotic trematodes (Heterophyidae: Centrocestus sp.) in Xiphophorus maculatus: Implications for ornamental fish import control in Europe Foojan Mehrdana, Hannah Jensen, Per Kania, Kurt Buchmann Acta Parasitologica.2014;[Epub] CrossRef
Trematodes Recovered in the Small Intestine of Stray Cats in the Republic of Korea Jong-Yil Chai, Young Yil Bahk, Woon-Mok Sohn The Korean Journal of Parasitology.2013; 51(1): 99. CrossRef
Foodborne Intestinal Flukes in Southeast Asia Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim The Korean Journal of Parasitology.2009; 47(Suppl): S69. CrossRef
Food-borne intestinal trematode infections in the Republic of Korea Jong-Yil Chai, Soon-Hyung Lee Parasitology International.2002; 51(2): 129. CrossRef
An epidemiological study and mass treatments of Enterobius vermicularis infection among children near Wonju area of Kangwon province were carried out. The children were divided into 4 groups according to their residing localities; children in the mountainous area, rural area, urban area and in orphanage. They were examined by adhesive cellotape anal swab technique, and egg positive rates were obtained. The rates of egg reduction and re-infection rates after repeated mass treatments were also observed. The results obtained were as follows: 1. The overall egg positive rate of E.
vermicularis in the first screening was 19.9% (251 out of 1,262 examinees; 19.7% in males and 20.1% in females). The positive rates were 13.0% in the mountainous area, 11.9% in the rural area, 15.1% in the urban (medium-sized) area and 61.9% in orphanages. 2. The highest positive rates were observed in the kindergarten children, and 1st and 2nd grade children of primary schools (26.2-32.2%), and the lowest rate (13.6%) in 6-year grade children of primary schools. 3.
Cumulative detection rates from 3 repeated anal swabs at 4-5 days interval were higher (70.8%) than those from single anal swabs (50.0-59.2%). 4. Out of the examinees who showed the highest cumulative positive rate (70.8%), about 39.2% were consecutively positive in 3 anal swabs. Among different groups of children, the higher the total egg detection rates (87.5%), the higher the consecutive positive rates (71.9%).
5. A total of 2,609 (male: female = 1:12.4) worms were collected from 17 egg-positive cases treated with anthelmintics. The mean number of worms per child was 153 (range: 4-824).(ABSTRACT TRUNCATED AT 250 WORDS)
Citations
Citations to this article as recorded by
E. VERMICULARIS – PROSPECTS FOR FUTURE RESEARCH Eleonora Kaneva, Rumen Harizanov, Nina Tsvetkova, Iskren Kaftanjiev, Raina Borisova, Aleksandra Ivanova, Mihaela Videnova, Desislava Velcheva, Ivailo Alexiev, Reneta Dimitrova, Maria Pavlova, Ekaterina Aleksandrova PROBLEMS of Infectious and Parasitic Diseases.2023; 50(2): 13. CrossRef
Epidemiologic Study of Enterobius vermicularis Infection among Schoolchildren in the Republic of Marshall Islands Chia-Kwung Fan, Pasaikou Sonko, Yueh-Lun Lee, Ai-Wen Yin, Ting-Wu Chuang, Ramson Kios, Ying-Ting Wang, Chia-Mei Chou, Shao-Lun Hsu, Mai-Szu Wu, Jia-Wei Lin, Chia-Ying Tu, Pedro P. Chieffi Journal of Tropical Medicine.2021; 2021: 1. CrossRef
Enterobius vermicularis Infection among Preschool Children: A 12-Year (2008-2019) Survey in Large Cities and Provinces of the Republic of Korea Hyejoo Shin, Bong-Kwang Jung, Seungwan Ryoo, Sooji Hong, Taehee Chang, Jiyeon Park, Keon Hoon Lee, Jeonggyu Lee, Jae Young Park, Hoo-Gn Jeoung, Jae Hyun Cho, Jong-Yil Chai The Korean Journal of Parasitology.2021; 59(4): 421. CrossRef
Negligible Egg Positive Rate of <i>Enterobius vermicularis</i> and No Detection of Head Lice among Orphanage Children in Busan and Ulsan, Korea (2014) Dong-Hee Kim, Hyun-Mi Son, Sang Hwa Lee, Mi Kyung Park, Shin Ae Kang, Sang Kyun Park, Jun-Ho Choi, Jung Ha Park, Hak-Sun Yu The Korean Journal of Parasitology.2015; 53(4): 497. CrossRef
Enterobius vermicularis: A Cause of Acute Appendicitis in Children Alexandra C. Maki, Barb Combs, Beth McClure, Pamela Slack, Paul Matheson, Chad Wiesenauer The American Surgeon™.2012; 78(12): 523. CrossRef
Comparison of Egg Positive Rates ofEnterobius vermicularisamong Preschool Children in Three Korean Localities Sung-Hee Hong, Sang-Eun Lee, Young-Il Jeong, Won-Ja Lee, Shin-Hyeong Cho The Korean Journal of Parasitology.2011; 49(4): 441. CrossRef
Effectiveness of Repeated Examination to Diagnose Enterobiasis in Nursery School Groups Mare Remm, Kalle Remm The Korean Journal of Parasitology.2009; 47(3): 235. CrossRef
Egg positive rate of Enterobius vermicularis among preschool children in Cheongju, Chungcheongbuk-do, Korea Seokha Kang, Hyeong Kyu Jeon, Keeseon S. Eom, Joong-Ki Park The Korean Journal of Parasitology.2006; 44(3): 247. CrossRef
The Relationship Between Enterobius Vermicularis Infection And Nocturnal Enuresis Gülnaz Çulha, Nizami Duran Electronic Journal of General Medicine.2006; 3(1): 16. CrossRef
A survey of Enterobius vermicularis infection among children on western and southern coastal islands of the Republic of Korea Jae-Hwan Park, Eun-Taek Han, Won-Hee Kim, Eun-Hee Shin, Sang-Mee Guk, Jae-Lip Kim, Jong-Yil Chai The Korean Journal of Parasitology.2005; 43(4): 129. CrossRef
Prevalence of intestinal parasitic infections and their relation with socio-economic factors and hygienic habits in Tehran primary school students Javad Nematian, Edwin Nematian, Ali Gholamrezanezhad, Ali Ali Asgari Acta Tropica.2004; 92(3): 179. CrossRef
Enterobius vermicularis egg positive rates in primary school children in Gangwon-do (Province), Korea Kyu-Jae Lee, Yung-Kyum Ahn, Yong-Suk Ryang The Korean Journal of Parasitology.2001; 39(4): 327. CrossRef
Intestinal parasite infections at an institution for the handicapped in Korea Jongweon Lee, Gab-Man Park, Du-Ho Lee, Soon-Jung Park, Tai-Soon Yong The Korean Journal of Parasitology.2000; 38(3): 179. CrossRef
Enterobius vermicularis egg positive rate in a primary school in Chungchongnam-do (Province) in Korea Kyu-Jae Lee, In-Yong Lee, Kyung-il Im The Korean Journal of Parasitology.2000; 38(3): 177. CrossRef
A survey of helminthic infections in the residents of rural areas near Ulaanbaatar, Mongolia Do-Sung Lee, Byung-ha Chung, Nam-seok Lee, Ho-woo Nam, Joong-ho Kim The Korean Journal of Parasitology.1999; 37(3): 145. CrossRef
Chemotherapeutic trial to control enterobiasis in schoolchildren Y S Yang, S W Kim, S H Jung, S Huh, J H Lee The Korean Journal of Parasitology.1997; 35(4): 265. CrossRef
Enterobius gregorii Hugot, 1983 recovered from school children in Kangwon-Do, Korea Y K Ahn, P R Chung, C T Soh The Korean Journal of Parasitology.1992; 30(3): 163. CrossRef
In order to determine the antigenic localization in the tissues of the adult Metagonimus yokogawai, immunogoldlabeling method was applied using serum immunoglobulins(IgG) of cats which were infected with isolated metacercariae from Plecoglossus altivelis. The sectioned worm tissue was embedded in Lowicryl HM 20 medium and stained with infected serum IgG and protein A gold complex(particle size: 12 nm). It was observed by electron microscopy at each tissue of the worm. The gold particles were observed on the tegumental syncytium as well as cytoplasm of tegumental cells and epithelial lamella of the caecum. The gold particles were not observed on the basal lamina of the tegument, interstitial matrix of the parenchyma, the muscle tissue and mitochondria of the tegument. The gold particles were specifically labeled in the secretory granules in the vitelline cells. They were also labeled on the lumen of bladder and egg shell. The above findings showed that antigenic materials in the tissue of adult worms were specifically concentrated on the tegumental syncytium as well as cytoplasm of tegumental cells and epithelial lamella of the caecum.
Citations
Citations to this article as recorded by
Fishborne zoonotic heterophyid infections: An update Jong-Yil Chai, Bong-Kwang Jung Food and Waterborne Parasitology.2017; 8-9: 33. CrossRef
Metagonimus yokogawai: a 100-kDa Somatic Antigen Commonly Reacting with Other Trematodes Eun-Taek Han, Hyun-Jong Yang, Young-Jin Park, Jeong-Hyun Park, Jong-Yil Chai The Korean Journal of Parasitology.2014; 52(2): 201. CrossRef
Foodborne Intestinal Flukes in Southeast Asia Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim The Korean Journal of Parasitology.2009; 47(Suppl): S69. CrossRef
Food-borne intestinal trematode infections in the Republic of Korea Jong-Yil Chai, Soon-Hyung Lee Parasitology International.2002; 51(2): 129. CrossRef
Localization of worm antigen in Neodiplostomum seoulense by immuno-electronmicroscopy J C Lee, Y Kong, S U Lee, S Huh The Korean Journal of Parasitology.1997; 35(2): 95. CrossRef
Ultrastructural antigenic localization in Paragonimus iloktsuenensis during developmental stage by immunogold labeling method H S Kim, O R Lee The Korean Journal of Parasitology.1995; 33(4): 365. CrossRef
Antigenic localities in the tissues of Metagonimus yokogawai in the period of growth H J Rim, S J Kim, M G Yang The Korean Journal of Parasitology.1992; 30(4): 309. CrossRef
Antigenic localities in the tissues of Paragonimus westermani by developmental stages using immunogold labeling method H J Rim, S J Kim, I J Sun, J S Lee The Korean Journal of Parasitology.1992; 30(1): 1. CrossRef
In cytosolic fraction of adult Paragonimus westermani, superoxide dismutase activity was identified (4.3 units/mg of specific activity) using a xanthine-xanthine oxidase system. The enzyme was purified 150 fold in its activity using the ammonium sulfate precipitation, DEAE-Trisacryl M anion-exchange chromatography and Sephadex G-100 molecular sieve chromatography. The enzyme exhibited the enhanced activity at pH 10.0. The enzyme activity totally disappeared in 1.0mM cyanide while it remained 77.8% even in 10 mM azide. These findings indicated that the enzyme was Cu, Zn-SOD type. Molecular mass of the enzyme was estimated to be 34 kDa by gel filtration and 17 kDa on reducing SDS-polyacrylamide gel electrophoresis which indicated a dimer protein.
Citations
Citations to this article as recorded by
Characterization of Superoxide Dismutase in the Metacestode of Echinococcus granulosus Sensu Stricto (s. s.) and Buffalo Liver Humera Aslam, Monika Rani, Malik Irshadullah Acta Parasitologica.2025;[Epub] CrossRef
Assessment of antioxidant enzymes during the development of the digenetic trematode parasite Gastrothylax crumenifer, infecting the rumen of Indian water buffalo, Bubalus bubalis Tahmina Zainab, Sabiha Khatoon, Anam Sahreen, Mohammad Khalid Saifullah Journal of Parasitic Diseases.2023; 47(2): 271. CrossRef
MOLECULAR CLONING AND CHARACTERIZATION OF COPPER/ZINC-SUPEROXIDE DISMUTASE OF PARAGONIMUS WESTERMANI Ai-Hua Li, Byoung-Kuk Na, Yoon Kong, Shin-Hyeong Cho, Qin-Ping Zhao, Tong-Soo Kim Journal of Parasitology.2005; 91(2): 293. CrossRef
Molecular cloning and characterization of the copper/zinc and manganese superoxide dismutase genes from the human parasiteClonorchis sinensis A. H. LI, Y. KONG, S. H. CHO, H. W. LEE, B. K. NA, J. K. PAK, T. S. KIM Parasitology.2005; 130(6): 687. CrossRef
Paragonimus westermani: A Cytosolic Glutathione S-Transferase of a σ-Class in Adult Stage Sung-Jong Hong, Shin-Yong Kang, Young-Bae Chung, Min-Hyun Chung, Youn-Jin Oh, Insug Kang, Young Yil Bahk, Yoon Kong, Seung-Yull Cho Experimental Parasitology.2000; 94(3): 180. CrossRef
Characterization of a peroxidase in excretory-secretory product of adult Paragonimus westermani Y B Chung, Y Kong, S Y Cho, S Y Kang, B C Choi, H S Lee The Korean Journal of Parasitology.1993; 31(3): 259. CrossRef
Activities of scavenging enzymes of oxygen radicals in early maturation stages of Paragonimus westermani Y B Chung, H S Lee, C Y Song, S Y Cho The Korean Journal of Parasitology.1992; 30(4): 355. CrossRef
Component proteins in crude extract of adult Paragonimus westermani purified by immunoaffinity chromatography using monoclonal antibodies S Y Kang, Y Kong, S Y Cho The Korean Journal of Parasitology.1991; 29(4): 363. CrossRef
The natural killer (NK) cell activity of splenocytes and recycling capacity of NK cells were observed by combining the 51Cr-release cytotoxicity assay and single cell cytotoxicity assay against YAC-1. The ICR mice were infected intranasally with Naegleria fowleri, that is a pathogenic free-living amoeba. The mice infected with 1 x 10(5) trophozoites showed mortality rate of 76.7% and mean survival time of 12.9 days. The cytotoxic activity of NK cells in infected mice was significantly higher than that of non-infected mice during the period between 12 hours and day 3 after infection, and highest on day 1. The target-binding capacity of NK cells in infected mice was not different from that of non-infected ones. Maximal killing potential and maximal recycling capacity were remarkably increased in infected mice as compared with the control. The results obtained in this observation indicated that elevated NK cell activity in mice infected with N. fowleri was not due to target-binding capacity of NK cells but due to the increased activity of NK cells and increased recycling capacity of individual NK cells.
Citations
Citations to this article as recorded by
Interleukin-2 production and alteration of T cell subsets in mice infected with Naegleria fowleri C J Lyu, C O Shin, K Im The Korean Journal of Parasitology.1993; 31(3): 249. CrossRef
Natural killer cell activity in mice infected with free-living amoeba with reference to their pathogenicity K H Kim, C O Shin, K Im The Korean Journal of Parasitology.1993; 31(3): 239. CrossRef
The immune response to sheep red blood cell (sRBC) was monitored in the mice infected with Ascaris suum or Trichinella spiralis. The effects of the infection with T.
spiralis or the injection with cyclophosphamide(CY) as an immunosuppression agent prior to challenge infection with the embryonated eggs of A. suum were monitored in mice by means of the level of infection with A. suum and cellular and humoral immune response to sRBC. Following the oral administration of 1,000 eggs of A. suum to mice, delayed-type hypersensitivity (DTH) and rosette-forming rate were gradually decreased and reached to the lowest levels at the 5th week and 6th week postinfection, respectively, and then returned to normal at the 10th week. The hemagglutinin(HA) and hemolysin(HE) titers were gradually elevated and reached to peak at the 3rd week postinfection, and then returned to normal level. The appearance ratios of the eosinophils and mast cells were in peak at the 4th week and the 2nd week postinfection, respectively. Meanwhile the harvest ratio of A. suum larvae from the liver and lungs was 21.97% at the 1st week postinfection. Following the oral administration of 300 T. spiralis infective larvae, DTH and rosette-forming rate were gradually decreased with the lapse of time and reached the lowest values in the 30th and 21st day of postinfection, and then slightly increased and transiently decreased in the 70th and 80th day of postinfection, respectively. HA and HE titers were the lowest in the 21st and 90th day, whereas the ratios of eosinophils and mast cells were the highest on the 40th and 14th day postinfection, respectively. Following the intraperitoneal injection of CY, the body weight, the spleen weight, DTH, rosette-forming ratio, HA and HE titers, the number of WBC and the ratio of the mast cell were predominantly decreased in the 5th day, and then returned to the same value of the 1st day postinjection. The ratio of eosinophils was gradually decreased following to advance of days. At the 1st, 5th and 10th days after intraperitoneal injection of CY of 400 mg/kg, a dose with 1,000 eggs of A.
suum was administered orally to mice, and harvest rate of the larvae at the 7th day postadministration was 7.07% in the 1st day, 14.94% in the 5th day, 10.1% in the 10th day, 8.02% in control group. The effect of prior infection with infective larvae of T. spiralis upon immunological sequelae of a challenge infection of mice with embryonated eggs of A.
suum in 30 or 70 days interval was checked.(ABSTRACT TRUNCATED AT 400 WORDS)
Citations
Citations to this article as recorded by
Effects of Cryptosporidium muris (strain MCR) infection on gastric mucosal mast cells in mice J K Rhee, C G Surl, H C Kim The Korean Journal of Parasitology.1997; 35(4): 245. CrossRef
Clonorchis sinensis is a common parasite of man in Korea.
Researches on the specific antigens of C. sinensis would be valuable not only because those elucidate the molecular characteristics of this fluke but also because it is applicable to immunodiagnosis. Although many monoclonal antibodies have been used in the field of parasite immunology, few articles on monoclonal antibodies against C.
sinensis have been published so far. The aim of this study was to analyze C. sinensis antigens recognized by monoclonal antibodies, and to set up ELISA-inhibition test using C.
sinensis specific monoclonal antibodies for improved specificity of immunodiagnostic tests. By fusion between spleen cells of the mice immunized with C. sinensis water-soluble crude adult worm antigens and plasmacytoma cells of mouse origin, 29 hybridoma clones secreting anti-C.
sinensis monoclonal antibodies were made, and 8 clones among those were found specific. After cell cloning, isotypes of 6 selected specific monoclonal antibodies were determined to be IgG1, IgG2b and IgA. Four exposed antigenic determinants of natural infection were recognized by different specific monoclonal antibodies. By enzyme-immunoelectrotransfer blot, 10 KD, 34 KD antigenic determinants were found to be reacted with CsHyb 0714-20, CsHyb 0605-10 monoclonal antibodies, respectively. The antigenic determinant recognized by CsHyb 0714-20 monoclonal antibody was revealed to be located at the surface and parenchyme of a parasite by indirect immunofluorescent antibody technique, and those reacted with CsHyb 0605-10, CsHyb 0714-25 monoclonal antibodies were found at the parenchyme and intestine. The antigenic determinant reacted with CsHyb 0605-23 monoclonal antibody was found mainly around the uterine eggs. Four antigenic determinants recognized by specific monoclonal antibodies were all found to be present in the early eluted fractions of C. sinensis antigens separated by Sephadex G-200 gel filtration. By conventional ELISA, 75% of clonorchiasis cases were found positive, but 7.1% of normal controls and 37.5% of paragonimiasis cases showed false positives.
However, by ELISA-inhibition test using C. sinensis specific monoclonal antibody (CsHyb 0605-23), 77.1% of clonorchiasis cases were found positive, and there were no false positives in normal controls or paragonimiasis cases, indicating 100% specificity.(ABSTRACT TRUNCATED AT 400 WORDS)
Citations
Citations to this article as recorded by
Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini (Opisthorchidae, Trematoda) Min-Ho Choi, Jin-Sook Ryu, Mejeong Lee, Shunyu Li, Byung-Suk Chung, Jong-Yil Chai, Paiboon Sithithaworn, Smarn Tesana, Sung-Tae Hong The Korean Journal of Parasitology.2003; 41(3): 155. CrossRef
PURIFICATION AND CHARACTERIZATION OF A 7-KDA PROTEIN FROM CLONORCHIS SINENSIS ADULT WORMS Hye-Jeong Lee, Chang-Seok Lee, Beom-Su Kim, Kyoung-Hwan Joo, Joon-Sang Lee, Tong-Soo Kim, Hak R. Kim Journal of Parasitology.2002; 88(3): 499. CrossRef
EXPRESSION OF CYSTEINE PROTEINASE OF CLONORCHIS SINENSIS AND ITS USE IN SERODIAGNOSIS OF CLONORCHIASIS Byoung-Kuk Na, Hye-Jeong Lee, Shin-Hyeong Cho, Hyeong-Woo Lee, Jung-Hwa Cho, Weon-Gyu Kho, Joon-Sang Lee, Jong-Soo Lee, Kyoung-Ju Song, Po-Hyun Park, Chul-Yong Song, Tong-Soo Kim Journal of Parasitology.2002; 88(5): 1000. CrossRef
Molecular cloning and immunological characterization of phosphoglycerate kinase from Clonorchis sinensis Sung-Jong Hong, Kee-Young Seong, Woon-Mok Sohn, Kye-Yong Song Molecular and Biochemical Parasitology.2000; 108(2): 207. CrossRef
Usefulness of IgG4 subclass antibodies for diagnosis of human clonorchiasis Sung-Tae Hong, Mejeong Lee, Nak-Jin Sung, Sang Rock Cho, Jong-Yil Chai, Soon-Hyung Lee The Korean Journal of Parasitology.1999; 37(4): 243. CrossRef
Monoclonal antibodies to recombinant Der p 2, a major house dust mite allergen: specificity, epitope analysis and development of two-site capture ELISA Tai-Soon Yong, Sang-Mi Lee, Gab-Man Park, In-Yong Lee, Han-Il Ree, Kyung-Sup Kim, Sang-Hwan Oh, Jung-Won Park, Chein-Soo Hong The Korean Journal of Parasitology.1999; 37(3): 163. CrossRef
Immunodiagnosis of clonorchiasis using a recombinant antigen T S Yong, H J Yang, S J Park, Y K Kim, D H Lee, S M Lee The Korean Journal of Parasitology.1998; 36(3): 183. CrossRef
A Clonorchis sinensis-specific antigen that detects active human clonorchiasis S I Kim The Korean Journal of Parasitology.1998; 36(1): 37. CrossRef
A carbohydrate antigen of Clonorchis sinensis recognized by a species-specific monoclonal antibody Tai Soon YONG, Jong Seog LEE, Sang Nae CHO, Jang Hoon SEO, Hyun PARK The Korean Journal of Parasitology.1996; 34(4): 279. CrossRef
Immune reactions between excretory-secretory antigens and specific antibodies of Clonorchis sinensis before and after praziquantel treatment in experimentally infected rabbits S I Kim The Korean Journal of Parasitology.1994; 32(1): 35. CrossRef
Serodiagnosis of cysticercosis by ELISA-inhibition test using monoclonal antibodies T S Yong, I S Yeo, J H Seo, J K Chang, J S Lee, T S Kim, G H Jeong The Korean Journal of Parasitology.1993; 31(2): 149. CrossRef
Serodiagnosis of human paragonimiasis by ELISA-inhibition test using monoclonal antibodies T S Yong, J H Seo, I S Yeo The Korean Journal of Parasitology.1993; 31(2): 141. CrossRef
This study revealed the infection status of P. parva with the metacercariae of M. orientalis in the Sunam stream of the Nakdong River, a well-known focus of Clonorchis sinensis. A total of 60 P. parva were divided into 4 groups by the size (80-90, 70-79, 60-69 and 50-59 mm) and were digested by pepsin-HCl solution in a 36 degrees C incubator.
The metacercariae of M. orientalis were collected and counted under a stereomicroscope. A total of 3,885 metacercariae were found, and average number/fish in each group were 143, 54, 23 and 40. They were 175.3 x 155.4 microns in average size and had thick cyst wall(13.1 microns in average). By the results, it is proved that the life cycle of M. orientalis is actively maintained in the Sunam stream of the Nakdong River.
Citations
Citations to this article as recorded by
Infection Status with Digenetic Trematode Metacercariae in Fishes from Coastal Lakes in Gangwon-do, Republic of Korea Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Soon-Won Lee The Korean Journal of Parasitology.2019; 57(6): 681. CrossRef
First
Prev
