A transmission electron microscopic study was performed to observe the ultrastructures of the male germinal cells and spermatozoa of Fibricola seoulensis. Spermatogonia were found in the periphery of the testis and characterized by large nuclei and comparatively little cytoplasms.
Spermatocytes contained an oval to spherical nucleus. Their nuclear volume was little larger in comparative to that of cytoplasm, and the chromatin was comparatively little. The early spermatids were characterized by a great amount of cytoplasm, and numerous mitochondria encircled the nucleus.
In a more advanced spermatids the electron-dense strands of chromatin appeared in the nucleus, and a pair of rootlet of the axoneme and a microtubule-organizing center (MTOC) were observed near the nucleus. The sectioned spermatozoa were found in the testis and the seminal vesicle. Their cross sectional views were divided into 6 types when they were distinguished on the basis of the morphology and components.
The spermatozoa of F. seoulensis showed two flagella of 9+1 type axoneme.
Citations
Citations to this article as recorded by
Spermiogenesis and spermatozoon of Echinostoma caproni (Platyhelminthes, Digenea): transmission and scanning electron microscopy, and tubulin immunocytochemistry C. Iomini, J.-L. Justine Tissue and Cell.1997; 29(1): 107. CrossRef
A total of 230 randomly collected formalin-fixed fecal samples (submitted to the Severance Hospital, Yonsei University) were selected for tests for human cryptosporidiosis. The stool specimens were examined for Cryptosporidium oocysts by acid-fast (AF) stain, auramin-rhodamine (AR) stain, and monoclonal antibody (mAb) OW3 fluorescence method specific for oocyst wall. Of the 230 stool specimens, 21% were identified by the AF method, 22% were identified by the AR method, and 10% were identified by the mAb fluorescence method, indicating that human Cryptosporidium infections have been existing in Korea.
Citations
Citations to this article as recorded by
Prevalence and Molecular Characterization of Cryptosporidium Species in Diarrheic Children in Cameroon Bertrand Sone, Lum Abienwi Ambe, Mireille Nguele Ampama, Constance Ajohkoh, Desmond Che, Julien Alban Nguinkal, Anja Taubert, Carlos Hermosilla, Faustin Kamena Pathogens.2025; 14(3): 287. CrossRef
Multilocus typing of Cryptosporidium spp. in young calves with diarrhea in Korea Seung-Hun Lee, Dorene VanBik, Ha-Young Kim, Yu-Ran Lee, Jong Wan Kim, Myeongju Chae, Sang-Ik Oh, Youn-Kyoung Goo, Oh-Deog Kwon, Dongmi Kwak Veterinary Parasitology.2016; 229: 81. CrossRef
Clinical Update on Parasitic Diseases Min Seo Korean Journal of Medicine.2013; 85(5): 469. CrossRef
A Waterborne Outbreak and Detection of Cryptosporidium Oocysts in Drinking Water of an Older High-Rise Apartment Complex in Seoul Eun-Joo Cho, Jin-Young Yang, Eun-Sook Lee, Se-Chul Kim, So-Yang Cha, Sung-Tek Kim, Man-Ho Lee, Sun-Hee Han, Young-Sang Park The Korean Journal of Parasitology.2013; 51(4): 461. CrossRef
Chemotherapeutic drugs for common parasitic diseases in Korea Sun Huh Journal of the Korean Medical Association.2013; 56(6): 513. CrossRef
Ultrastructural Localization of Cryptosporidium parvum Antigen Using Human Patients Sera Jong-Gyu Lee, Eun-Taek Han, Woo-Yoon Park, Jae-Ran Yu The Korean Journal of Parasitology.2009; 47(2): 171. CrossRef
A Hospital-Based Serological Survey of Cryptosporidiosis in the Republic of Korea Jong-Kyu Lee, Eun-Taek Han, Sun Huh, Woo-Yoon Park, Jae-Ran Yu The Korean Journal of Parasitology.2009; 47(3): 219. CrossRef
Emerging Parasitic Diseases in Korea Jong-Yil Chai Journal of the Korean Medical Association.2007; 50(11): 946. CrossRef
A survey of cryptosporidiosis among 2,541 residents of 25 coastal islands in Jeollanam-do (Province), Republic of Korea Jae-Hwan Park, Hyo-Jin Kim, Sang-Mee Guk, Eun-Hee Shin, Jae-Lip Kim, Han-Jong Rim, Soon-Hyung Lee, Jong-Yil Chai The Korean Journal of Parasitology.2006; 44(4): 367. CrossRef
Prevalence of cryptosporidiosis among the villagers and domestic animals in several rural areas of Korea Jae-Ran Yu, Jong-Kyu Lee, Min Seo, Seok-Il Kim, Woon-Mok Sohn, Sun Huh, Hae-Yeon Choi, Tong-Soo Kim The Korean Journal of Parasitology.2004; 42(1): 1. CrossRef
Infection status of pigs with Cryptosporidium parvum Jae-Ran Yu, Min Seo The Korean Journal of Parasitology.2004; 42(1): 45. CrossRef
Detection of Cryptosporidium oocysts in canine fecal samples by immunofluorescence assay J T Kim, S H Wee, C G Lee The Korean Journal of Parasitology.1998; 36(2): 147. CrossRef
Localization of cytoskeletal proteins in Cryptosporidium parvum using double immunogold labeling Jae Ran YU, Soon Hyung LEE The Korean Journal of Parasitology.1996; 34(4): 215. CrossRef
Evaluation for detection of Cryptosporidium oocysts in diarrheal feces of calves S H Wee, H D Joo, Y B Kang The Korean Journal of Parasitology.1996; 34(2): 121. CrossRef
An epidemiological survey of Cryptosporidium parvum infection in randomly selected inhabitants of Seoul and Chollanam-do J Y Chai, S H Lee, S M Guk, S H Lee The Korean Journal of Parasitology.1996; 34(2): 113. CrossRef
Based on the morphologic characteristics of oocysts of the genus Isospora, we have demonstrated that I. ohioensis is a relatively common Isospora species in dogs which are resident in Chonbuk province, Korea. The prepatent period of I. ohioensis was four days. The size of the unsporulated oocysts in fresh stool specimens was 22.9 x 19.8 microns (R = 1.16). The size of the contracted sporonts was 17.4 x 16.3 microns (R = 1.06). By 96 hours, sporulation is complete and the ratio of length/width was constant relatively. And the sizes of oocysts and sporocysts were 22.8 x 20.5 microns (R = 1.11) and 15.0 x 10.8 microns (R = 1.39), respectively. At this time it is most reliable for the measurements of oocyst sizes of I. ohioensis to provide with the identifiable clues against the others. It is therefore recommended that the clinical fecal specimens suspected of isosporosis should first be incubated and aerated for 96 hours before a definitive parasitological diagnosis can be reached. These and other observations contribute to the understanding of the biological characteristics and laboratory and clinical diagnosis of isosporosis.
Citations
Citations to this article as recorded by
Prevalence of Gastrointestinal Parasites in the Frugivorous and the Insectivorous Bats in Southcentral Nepal Roshan Babu Adhikari, Mahendra Maharjan, Tirth Raj Ghimire, Emmanuel Serrano Ferron Journal of Parasitology Research.2020; 2020: 1. CrossRef
Coccidiosis in dogs—100 years of progress Jitender P. Dubey, David S. Lindsay Veterinary Parasitology.2019; 266: 34. CrossRef
Identification of Cystoisospora ohioensis in a Diarrheal Dog in Korea Sangmin Lee, Junki Kim, Doo-Sung Cheon, Eun-A Moon, Dong Joo Seo, Soontag Jung, Hansaem Shin, Changsun Choi The Korean Journal of Parasitology.2018; 56(4): 371. CrossRef
Successful therapeutic management of canine Isosporosis in puppies Nishiswapna Garanayak, A. R. Gupta, R. C. Patra Journal of Parasitic Diseases.2017; 41(1): 48. CrossRef
Influence of Breed Size, Age, Fecal Quality, and Enteropathogen Shedding on Fecal Calprotectin and Immunoglobulin A Concentrations in Puppies During the Weaning Period A. Grellet, R.M. Heilmann, B. Polack, A. Feugier, C. Boucraut‐Baralon, D. Grandjean, N. Grützner, J. S. Suchodolski, J.M. Steiner, S. Chastant‐Maillard Journal of Veterinary Internal Medicine.2016; 30(4): 1056. CrossRef
Use of ponazuril paste to treat coccidiosis in shelter-housed cats and dogs A.L. Litster, J. Nichols, K. Hall, J. Camp, A.S. Mohamed Veterinary Parasitology.2014; 202(3-4): 319. CrossRef
Risk factors of weaning diarrhea in puppies housed in breeding kennels Aurélien Grellet, Sylvie Chastant-Maillard, Coralie Robin, Alexandre Feugier, Cassandre Boogaerts, Corine Boucraut-Baralon, Dominique Grandjean, Bruno Polack Preventive Veterinary Medicine.2014; 117(1): 260. CrossRef
Prevalence, risk factors of infection and molecular characterization of trichomonads in puppies from French breeding kennels Aurélien Grellet, BrunoPolack, Alexandre Feugier, Corine Boucraut-Baralon, Dominique Grandjean, Laurine Vandewynckel, Amandine Cian, Dionigia Meloni, Eric Viscogliosi Veterinary Parasitology.2013; 197(3-4): 418. CrossRef
Efficacy of Emodepside plus Toltrazuril Suspension (Procox® Oral Suspension for Dogs) against Prepatent and Patent Infection with Isospora canis and Isospora ohioensis-Complex in Dogs Gertraut Altreuther, Nadine Gasda, Iris Schroeder, Anja Joachim, Terry Settje, Annette Schimmel, Douglas Hutchens, Klemens J. Krieger Parasitology Research.2011; 109(S1): 9. CrossRef
Coyotes (Canis latrans) are definitive hosts of Neospora caninum Luis F.P Gondim, Milton M McAllister, William C Pitt, Doris E Zemlicka International Journal for Parasitology.2004; 34(2): 159. CrossRef
This study was conducted to evaluate the current endemicity and the transition mode of the Clonorchis infection in Sanchong-gun Kyongsangnam-do. The areas of investigation, villages and schools surveyed, method and techniques used in this study were the same as in 1984. The egg positive rate of clonorchiasis in the general population of Sanchong-gun was 37.6% out of 837 examined persons. In the schoolchildren, the rate was 9.7% out of 145 persons examined. In the intensity of the infection among the cases, the mean EPG (Egg per gram of feces) in the inhabitants and schoolchildren were 3,310 (male 4,221, female 1,978) and 711 (male 620, female 833), respectively. Of the inhabitants with clonorchiasis, the cases of light infection (EPG < 1,000) and moderate infection (1,000 < EPG < 10,000) were 93.9%. The proportion with experience of eating raw freshwater-fish was 90.5% among the infecteds. Out of 138 inhabitants examined in both 1984 and 1992, the number of the positives by both examinations was 31 (22.5%), positive to negative conversion was 35 (25.4%), negative to positive conversion was 22 (15.9%). The present results reveal that there are no changes of the egg positive rate of clonorchiasis among inhabitants in Sanchong and the eating habit of raw freshwater-fish persists there. In this community, the eating habit is the most important problem to be solved for control of this endemic disease.
Citations
Citations to this article as recorded by
Infection Characteristics of Clonorchis sinensis Metacercariae in Fish from Republic of Korea Woon-Mok Sohn The Korean Journal of Parasitology.2022; 60(2): 79. CrossRef
Endemicity of Zoonotic Trematode Metacercariae in Fish from Deokcheon-gang (River) in Sancheong-gun, Gyeongsangnam-do, Republic of Korea Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Hee Il Lee, Jung-Won Ju, Myoung-Ro Lee, Jeong-Gil Park, Jihee Ahn The Korean Journal of Parasitology.2021; 59(5): 523. CrossRef
Infection Status with Clonorchis sinensis Metacercariae in Fish from Yangcheon (Stream) in Sancheong-gun, Gyeongsangnam-do, Korea Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Jung-Won Ju The Korean Journal of Parasitology.2019; 57(2): 145. CrossRef
Analysis of Parasitic Diseases Diagnosed by Tissue Biopsy Specimens at KyungHee Medical Center (1984-2005) in Seoul, Korea Won-Hyung Choi, Jong-Phil Chu, Meihua Jiang, Yun-Sik Lee, Bum-Shik Kim, Deog-Gon Kim, Yong-Koo Park The Korean Journal of Parasitology.2010; 48(1): 85. CrossRef
Infection status of Clonorchis sinensis in residents of Hamyang-gun, Gyeongsangnam-do, Korea Bong Jin Kim, Mee-Sun Ock, Ik Su Kim, Un Bo Yeo The Korean Journal of Parasitology.2002; 40(4): 191. CrossRef
Infection rates of Enterobius vermtcularis and Clonorchis sinensis of primary school children in Hamyang-gun, Gyeongsangnam-do (Province), Korea Bong Jin Kim, Je Wook Yeon, Mee Sun Ock The Korean Journal of Parasitology.2001; 39(4): 323. CrossRef
Intestinal parasite infections in the inhabitants along the Hantan River, Chorwon-gun M S Park, S W Kim, Y S Yang, C H Park, W T Lee, C U Kim, E M Lee, S U Lee, S Huh The Korean Journal of Parasitology.1993; 31(4): 375. CrossRef
Intestinal parasitic infections of Korean Army soldiers in Whachon-gun, Korea S Huh, S C Huh The Korean Journal of Parasitology.1993; 31(3): 293. CrossRef
In order to establish a useful cell culture system for T.
gondii, we compared the degree of proliferation of T. gondii tachyzoites among 8 different cell lines; 2 kinds of normal animal cells (MDCK-canine kidney cells; Vero-monkey kidney cells) and 6 kinds of human tumor cells (A 549, PC 14-lung cancer cells; SNU 1, SNU 16, MKN 45-stomach cancer cells; HL-60-promyelocytic leukemia cells), through morphological observation and 3H-uracil uptake assay. The degree of susceptibility to infection with T. gondii tachyzoites was highest in A 549 and PC 14 cells, medium in Vero, HL-60, MDCK and SNU 1, and lowest in SNU 16 and MKN 45 cells. The kinetics of T. gondii multiplication during the post-infection 60 hours were highly dependent upon the dose of tachyzoites administered and the duration of cultivation.
These results show that A 549 and PC 14 are the most suitable cell lines among the 8 tested for the growth and multiplication of T. gondii in vitro.
Citations
Citations to this article as recorded by
Cytotoxic Effects of Ardisiacrispin A from Labisia pumila on A549 Human Lung Cancer Cells Yeong-Geun Lee, Tae Hyun Kim, Jeong Eun Kwon, Hyunggun Kim, Se Chan Kang Life.2024; 14(2): 276. CrossRef
Trans vaccenic acid (trans-11 18:1), a precursor of cis-9, trans-11-conjugated linoleic acid, exerts a direct anti-carcinogenic function in T47D breast carcinoma cells Jin-Ju Oh, Jae-Sung Lee, Ji-Na Lim, Tao Wang, Sang-Hun Kim, Hong-Gu Lee Food Science and Biotechnology.2014; 23(2): 641. CrossRef
Toxoplasma gondii: Virulence of tachyzoites in serum free media at different temperatures M.R. Diab, M.M. El-Bahy Experimental Parasitology.2008; 118(1): 75. CrossRef
Biology of SNU Cell Lines Ja-Lok Ku, Jae-Gahb Park Cancer Research and Treatment.2005; 37(1): 1. CrossRef
USE OF MONOCLONAL ANTIBODIES FOR FLOW CYTOMETRIC DETECTION OF INTRACELLULAR TOXOPLASMA GONDII IN MURINE SPLENIC LYMPHOCYTES Eun-Hee Shin, Sung-Bum Kim, Ho-Woo Nam, Eun-Taek Han, Jae-Hwan Park, Hye-Jin Ahn, Jong-Yil Chai Journal of Parasitology.2004; 90(1): 161. CrossRef
Experimental infection of murine splenic lymphocytes and granulocytes with Toxoplasma gondii RH tachyzoites J Y Chai, J Kook, S M Guk, Y P Chang, C K Yun The Korean Journal of Parasitology.1997; 35(2): 79. CrossRef
Monoclonal antibodies (mAb) against merozoites and sporozoites of the protozoan parasite Cryptosporidium parvum were examined for potential modulation of cryptosporidial infections in vivo by daily oral mAb administration to oocyst-inoculated neonatal mice. Monoclonal-treated neonatal mice were sacrificed four and eight days post infection (pi). Differences in infection rates were observed among the treatment groups at the p < 0.05 level. Suckling mice treated daily with orally administered mixtures of mAbs (ascitic fluids) showed significantly reduced parasite loads compared to control mice at four and eight days pi, while suckling mice receiving mAb Cmg-3 alone showed significant differences only at 4 days pi., suggesting that passive transfer of mAb may be of value in controlling cryptosporidial infections.
Citations
Citations to this article as recorded by
Parasite and host immune factors that impact the development of a mucosal vaccine for Cryptosporidium Maria Merolle, Boris Striepen, Christopher A. Hunter Mucosal Immunology.2025; 18(4): 782. CrossRef
Enteral human serum immunoglobulin treatment of cryptosporidiosis in mice with severe combined immunodeficiency T L Kuhls, S L Orlicek, D A Mosier, D L Crawford, V L Abrams, R A Greenfield Infection and Immunity.1995; 63(9): 3582. CrossRef
Effects of Paragonims westermani infection were observed in mice on the change of serum IgE level, the number of peripheral eosinophils and the distribution of Thy 1.2+ (CD3), L3T4+ (CD4), and Lyt-2+ (CD8) splenic T lymphocytes without mitogen stimulation. BALB/c mice were infected with 20 metacercariae of P. westermani each. Total serum IgE increased at 3 weeks after the infection and reached a peak on week 4 and maintained high levels of IgE until the 23rd week. Peripheral eosinophil numbers increased at the second week and attained peak level on week 9. The frequency of L3T4+ (CD4) and Lyt-2+ (CD8) T lymphocytes decreased slightly until 4 weeks after the infection, but not significantly. Absolute number of L3T4+ and Lyt-2+ T lymphocytes, and the ratio of L3T4/Lyt-2 were not markedly changed over the period of observation. The frequency of Thy 1.2+ (CD3) T lymphocytes in the infected group slightly decreased until 4 weeks after the infection and showed significant reductions at the 2nd and 4th week of the infection (p < 0.05).
Citations
Citations to this article as recorded by
Strain variation in the susceptibility and immune response to Clonorchis sinensis infection in mice Md. Hafiz Uddin, Shunyu Li, Young Mee Bae, Min-Ho Choi, Sung-Tae Hong Parasitology International.2012; 61(1): 118. CrossRef
Eosinophil and Tissue-invasive Parasitic Helminth Myeong Heon Shin Hanyang Medical Reviews.2010; 30(3): 238. CrossRef
Dermal mast cell responses in Paragonimus westermani-infected mice M H Shin The Korean Journal of Parasitology.1997; 35(4): 259. CrossRef
Effects of anti-IgE mAb on serum IgE, FcεRII/CD23 expression on splenic B cells and worm burden in mice infected with Paragonimus westermani M H Shin, H K Min The Korean Journal of Parasitology.1997; 35(1): 47. CrossRef
Production of interferon-γ and interleukin-4 by splenocytes in mice infected with Paragonimus westermani M H Shin, D Y Min The Korean Journal of Parasitology.1996; 34(3): 185. CrossRef
The purpose of this observation was to investigate the natural killer cell activities in mice infected with pathogenic free-living amoeba, Naegleria fowleri and Acanthamoeba culbertsoni according to the infection doses, and infected with non-pathogenic free-living amoeba, Naegleria gruberi. The natural killer cell activity was examined by means of target binding capacity, active NK cell and maximum recycling capacity of the mice after inoculating free-living amoebae with low and high doses. The mice infected with 1,103, 1,105 A. culbertsoni trophozoites showed mortality rates of 6.9% and 65.5%, respectively. The mice infected with 1,104, 1,105 N. fowleri trophozoites showed mortality rates of 5.9% and 72.2%, respectively. The NK cell activities in all experimental groups increased significantly on day 1 after infection as compared with control group, and then remarkably declined thereafter, there was no difference of the cytotoxic activity of the NK cells in mice among inoculation doses of pathogenic free-living amoebae. The target binding capacities of NK cells and percentages of activated NK cells in mice infected with pathogenic free-living amoebae were significantly increased a day after infection, as compared with control group. There was no difference of the maximal recycling capacities of NK cells in all experimental groups as compared with control group. There was significant difference in the cytotoxic activity and single cell cytotoxicity of NK cells between the experimental groups infected with pathogenic free-living amoebae and that infected with non-pathogenic free-living amoebae.
Citations
Citations to this article as recorded by
Various brain-eating amoebae: the protozoa, the pathogenesis, and the disease Hongze Zhang, Xunjia Cheng Frontiers of Medicine.2021; 15(6): 842. CrossRef
Acanthamoeba keratitis: A clinicopathologic case report and review of the literature Jared E. Knickelbein, Jessica Kovarik, Deepinder K. Dhaliwal, Charleen T. Chu Human Pathology.2013; 44(5): 918. CrossRef
The dynamic interdependence of amebiasis, innate immunity, and undernutrition Hans P. Verkerke, William A. Petri, Chelsea S. Marie Seminars in Immunopathology.2012; 34(6): 771. CrossRef
Biology and pathogenesis of Acanthamoeba Ruqaiyyah Siddiqui, Naveed Ahmed Khan Parasites & Vectors.2012;[Epub] CrossRef
Crosstalk at the initial encounter: interplay between host defense and ameba survival strategies Xiaoti Guo, Eric Houpt, William A. Petri Current Opinion in Immunology.2007; 19(4): 376. CrossRef
Acanthamoeba: biology and increasing importance in human health Naveed Ahmed Khan FEMS Microbiology Reviews.2006; 30(4): 564. CrossRef
Pathogenic free-living amoebae in Korea Ho-Joon Shin, Kyung-il Im The Korean Journal of Parasitology.2004; 42(3): 93. CrossRef
Interleukin-2 production and alteration of T cell subsets in mice infected with Naegleria fowleri C J Lyu, C O Shin, K Im The Korean Journal of Parasitology.1993; 31(3): 249. CrossRef
Naegleria fowleri is the cause of primary amoebic meningoencephalitis in man. IL-2 levels after stimulation of T lymphocytes by PHA or N. fowleri lysates, the amounts of T lymphocyte subsets and the blastogenic responses of T lymphocytes in mice after infected with pathogenic N.
fowleri were studied comparing between two study groups, one 1 x 10(4) trophozoites inoculated mice and the other 1 x 10(5) trophozoites inoculated mice. All experimental samples were obtained on the day 7, 14 and 24 after inoculation. The mice inoculated with 1 x 10(4) trophozoites showed a 14.3% mortality rate, and 72.2% in the mice inoculated with 1 x 10(5) trophozoites. The IL-2 levels on day 14 of two experimental groups were significantly decreased as compared with the control group. Thy 1.2+ T cells in the total spleen lymphocytes of 1 x 10(5) trophozoites inoculated group on day 7 were significantly increased compared with the control group. There was no significant difference between 1 x 10(4) trophozoites inoculated group and the control group. L3T4+ T cells and Ly2+ T cells in the total spleen lymphocytes of 1 x 105 trophozoites inoculated group on day 7 were significantly increased compared with the control group. The DNA S fraction of T cells in the spleen of 1 x 10(5) trophozoites inoculated group was significantly increased on day 7. The amount of S fractions of DNA were sequentially decreased on day 14 and 24, but they were also significantly increased compared with the control group.(ABSTRACT TRUNCATED AT 250 WORDS)
Citations
Citations to this article as recorded by
Pathogenic free-living amoebae in Korea Ho-Joon Shin, Kyung-il Im The Korean Journal of Parasitology.2004; 42(3): 93. CrossRef
When activity of peroxidase in adult Paragonimus westermani was monitored using o-dianisidine and H2O2 as substrates, its specific activity was 1.5 times higher in excretory-secretory product (ESP) than in crude extract. The enzyme was purified by two purification steps of Sephacryl S-300 Superfine gel permeation and DEAE-Trisacryl M anion exchange chromatographies. Its activity increased 16.9 fold with 32.3% recovery. The enzyme was inhibited totally by 1 millimoles of dithiothreitol (DTT), 2-mercaptoethanol and azide. Molecular mass was 16 kDa in reducing SDS-polyacrylamide gel electrophoresis (PAGE) or 19 kDa in TSK-Blue gel filtration high performance liquid chromatography (HPLC), respectively. Special staining for peroxidase by diaminobenzidine on SDS-PAGE confirmed the activity. The peroxidase was less reactive to a paragonimiasis serum when observed by SDS-PAGE/immunoblot.
In addition, specific activities of superoxide dismutase (SOD) and catalase were also identified in the ESP. High activities of these antioxidant enzymes in ESP indicate that they are parts of defense mechanisms against reactive oxygen intermediates from host.
Citations
Citations to this article as recorded by
Partial characterization of superoxide dismutase activity in the Barber pole worm-Haemonchus contortus infecting Capra hircus and abomasal tissue extracts Sadia Rashid, Malik Irshadullah Asian Pacific Journal of Tropical Biomedicine.2014; 4(9): 718. CrossRef
Molecular cloning and characterization of a major egg antigen in Paragonimus westermani and its use in ELISA for the immunodiagnosis of paragonimiasis Jae Sik Lee, Jongweon Lee, Sun Hyun Kim, Tai-Soon Yong Parasitology Research.2007; 100(4): 677. CrossRef
To ascertain that tegument of Paragonimus westermani has specific antigenic proteins, the tegumental fraction was isolated from 10-month-old worms by 0.1% digitonin solution, and subjected to SDS-PAGE and immunoblot. Component proteins of tegumental syncytium comprised of 94, 74 (76-66), 62, 54, 44, 42, 38, 28, 26, 25, 24, 17, 15.5 and 13. 5 kDa proteins.
Of them, the 94, 44 and 42 kDa proteins were more specific to tegument, especially the 94 kDa protein was the most prevailing one. In immunoblot, antigens of the 94, 90, 78, 76, 74, 68, 65, 62, 60, 59 and 54 kDa proteins were commonly detected by 7 sera of 10 human paragonimiasis, but none of them reacted with 5 sera of clonorchiasis. In conclusion, the 94 kDa protein was the major tegumental protein, as well as the specific antigen. The 76 and 66 kDa proteins were the minor components of tegument, which were also specific antigens of P. westermani.
Citations
Citations to this article as recorded by
Production of Polyclonal Antibodies against the Tegument of Sparganum (Plerocercoid of Spirometra mansoni) and Its Immunolocalization Hyun-Jong Yang The Korean Journal of Parasitology.2010; 48(2): 183. CrossRef
Distomatose pulmonaire ou paragonimose Thai Kien EMC - Biologie Médicale.2006; 1(1): 1. CrossRef
Leishmaniasis is one of the important tropical diseases in the world. Although it is not prevalent in Korea, imported cases have been recorded. The karyotype of Leishmania sp.
has been observed to be variable by localities or by strains, but the karyotype of a strain is known to be stable. This study was performed to observe if the karyotype of a Leishmania sp. would be changed under some stressful conditions. The karyotype, analyzed by pulsed field gradient gel electrophoresis, was not grossly changed by heat shock, chemotherapeutics, UV illumination, and gamma irradiation.
Radiation destroyed the chromosomes mechanically, but subcultured organisms after irradiation showed unaffected karyotype. The present findings suggest that the karyotype of a Leishmania strain is so stable that it is not altered by temporary stimulation with heat, drugs, and radiation.
Citations
Citations to this article as recorded by
Trypanosoma cruzi Gene Expression in Response to Gamma Radiation Priscila Grynberg, Danielle Gomes Passos-Silva, Marina de Moraes Mourão, Roberto Hirata Jr, Andrea Mara Macedo, Carlos Renato Machado, Daniella Castanheira Bartholomeu, Glória Regina Franco, Matty Knight PLoS ONE.2012; 7(1): e29596. CrossRef
Overview of DNA Repair in Trypanosoma cruzi, Trypanosoma brucei, and Leishmania major Danielle Gomes Passos-Silva, Matheus Andrade Rajão, Pedro Henrique Nascimento de Aguiar, João Pedro Vieira-da-Rocha, Carlos Renato Machado, Carolina Furtado, Ashis Basu Journal of Nucleic Acids.2010;[Epub] CrossRef
C. sinensis total RNA was containing large amount of 18S rRNA but little 28S rRNA. The size of the double-stranded cDNA synthesized from poly (A)+ mRNA was 0.4-4.2 kb long with tapering upto 9.5 kb. Degenerated oligonucleotides (as 2 sense and 3 antisense primers) were designed on the conserved regions of the known tropomyosin amino acid sequences. From one out of the PCR amplifications using total cDNA and matrix of primers, a specific gene product, 580 bp in size, was produced. Upon Southern hybridization of the PCR products with Schistosoma mansoni tropomyosin (SMTM) cDNA, only one signal appeared at the band of 580 bp product. This 580 bp product was considered to encode C.
sinensis tropomyosin (CSTM) and cloned in pGEM-3Zf(-) for DNA sequencing. CSTM cDNA was 575 bp containing one open reading frame of 191 predicted amino acids, which revealed 86.3% homology with SMTM and 51.1% with Trichostrongylus colubriformis tropomyosin. CSTM cDNA obtained will serve as a probe in the studies of molecular cloning of CSTM.
Citations
Citations to this article as recorded by
Progress on the transcriptomics of carcinogenic liver flukes of humans—Unique biological and biotechnological prospects Neil D. Young, Aaron R. Jex, Cinzia Cantacessi, Bronwyn E. Campbell, Thewarach Laha, Woon-Mok Sohn, Banchob Sripa, Alex Loukas, Paul J. Brindley, Robin B. Gasser Biotechnology Advances.2010; 28(6): 859. CrossRef
Reference genes for quantitative analysis on Clonorchis sinensis gene expression by real-time PCR Won Gi Yoo, Tae Im Kim, Shunyu Li, Oh Sil Kwon, Pyo Yun Cho, Tong-Soo Kim, Kijeong Kim, Sung-Jong Hong Parasitology Research.2009; 104(2): 321. CrossRef
Molecular cloning and immunological characterization of phosphoglycerate kinase from Clonorchis sinensis Sung-Jong Hong, Kee-Young Seong, Woon-Mok Sohn, Kye-Yong Song Molecular and Biochemical Parasitology.2000; 108(2): 207. CrossRef
Immunodiagnosis of clonorchiasis using a recombinant antigen T S Yong, H J Yang, S J Park, Y K Kim, D H Lee, S M Lee The Korean Journal of Parasitology.1998; 36(3): 183. CrossRef
Immune reactions between excretory-secretory antigens and specific antibodies of Clonorchis sinensis before and after praziquantel treatment in experimentally infected rabbits S I Kim The Korean Journal of Parasitology.1994; 32(1): 35. CrossRef
We examined stools of 113 soldiers of Korean Army in Whachon-gun, Korea in 1992. The eggs of Clonorchis sinensis (7/113) and Metagonimus sp. (3/113), and cysts of Giardia lamblia (4/113) were detected. No eggs of soil-transmitted helminths were found. Most of the Clonorchis-infected soldiers (6/7) were recruited from Kyongsangnam-do.
Citations
Citations to this article as recorded by
Status of Helminthic Infections in Residents around River Basins in the Republic of Korea for 10 Years (2011-2020) Myoung-Ro Lee, Hee-Eun Shin, Seon-Ok Back, Young-Ju Lee, Hee-Il Lee, Jung-Won Ju The Korean Journal of Parasitology.2022; 60(3): 187. CrossRef
Review of Zoonotic Parasites in Medical and Veterinary Fields in the Republic of Korea Heejeong Youn The Korean Journal of Parasitology.2009; 47(Suppl): S133. CrossRef
Infection status of Clonorchis sinensis in residents of Hamyang-gun, Gyeongsangnam-do, Korea Bong Jin Kim, Mee-Sun Ock, Ik Su Kim, Un Bo Yeo The Korean Journal of Parasitology.2002; 40(4): 191. CrossRef
A follow-up examination of intestinal parasitic infections of the Army soldiers in Whachon-gun, Korea S Huh, S U Lee, S C Huh The Korean Journal of Parasitology.1994; 32(1): 61. CrossRef
Paragonimus westermani is a lung fluke of humans that is usually found in the lungs but may be found elsewhere in many unusual locations. A case of pelvic paragonimiasis was found incidentally by surgical intervention of inflammatory disease and myoma uteri. She was a 51-year-old Korean woman complaining of lower abdominal pain and intermittent vaginal spotting. Numerous Paragonimus ova were observed in the resected omentum in the pelvis after total abdominal hysterectomy. It is suggested that pelvic paragonimiasis may be one of causative agents of pelvic inflammatory disease.
Citations
Citations to this article as recorded by
The Quality of Scutellaria baicalensis Georgi Is Effectively Affected by Lithology and Soil’s Rare Earth Elements (REEs) Concentration Zijian Sun, Wei Shen, Weixuan Fang, Huiqiong Zhang, Ziran Chen, Lianghui Xiong, Tianhao An Applied Sciences.2023; 13(5): 3086. CrossRef
A Case of Ectopic Peritoneal Paragonimiasis Mimicking Diverticulitis or Abdominal Abscess Min Jae Kim, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Jun Hee Woo, Yong Sik Yoon, Kyung Won Kim, Jaeeun Cho, Jong-Yil Chai, Yong Pil Chong The Korean Journal of Parasitology.2017; 55(3): 313. CrossRef
Effect of TiO2addition to LF refining slag on the Ti, Al, and cleanliness of Ti-stabilized stainless steel G.-Y. Qian, F. Jiang, G.-G. Cheng, C.-S. Wang Metallurgical Research & Technology.2014; 111(4): 229. CrossRef
A Case of Ovarian Paragonimiasis Mimicking Ovarian Carcinoma Charuwan Tantipalakorn, Surapan Khunamornpong, Theera Tongsong Gynecologic and Obstetric Investigation.2014; 77(4): 261. CrossRef
A Case of Intra-abdominal Heterotopic Paragonimiasis Combined with Rectal Cancer Soo Hong Kim, Hyung Jin Kim, Jae Im Lee, Bong Hyeon Kye, Soon Nam Oh, Chan Kwon Jung, Won Kyung Kang, Jun Gi Kim, Seong Taek Oh Journal of the Korean Society of Coloproctology.2010; 26(2): 157. CrossRef
Paragonimiasis: a Japanese perspective Fukumi Nakamura-Uchiyama, Hiroshi Mukae, Yukifumi Nawa Clinics in Chest Medicine.2002; 23(2): 409. CrossRef
Heterotopic Paragonimiasis in the Omentum Woo Kyeong Jeong, Yongsoo Kim, Young-Sun Kim, Dong-Woo Park, Choong-Ki Park, Hong-Kyu Baek, Yong Wook Park Journal of Computer Assisted Tomography.2002; 26(6): 1019. CrossRef
A cysteine protease of Paragonimus westermani eggs S Y Kang, M S Cho, Y B Chung, Y Kong, S Y Cho The Korean Journal of Parasitology.1995; 33(4): 323. CrossRef
First
Prev
