Author studied on the incidence of intestinal parasites with fecal examination on the out patient Pusan University Hospital, between from January to December 1969. The method employed were Formalin-Ether and flotation method in 3,510 for the prevalence rate of intestinal helminthes(Ascaris lumbricoides, hookworm, Trichocephalus trichiurus, Trichostrongylus orientalis). The following results were obtained: The positive rate of intestinal helminthes of total was 65.4 percent among 3,510 persons. The infection rate of Ascaris lumbricoides, hookworm, Trichocephalus trichiurus, Trichostrongylus orientalis were 19.3 %, 6.5 %, 55.2 % and 46.1 %. The female (66.4%) had definitely higher infection rate than male (65.0 %). The age group of 51-60 year of the male showed highest infection rate 75.4 % and the age group 31-40 year of the female showed highest infection rate 72.9 %.
The 4,725 cattle in the enzootic region, 6 counties of southern Korea, were surveyed for besnoitiosis epidemiologically by examining the scleral conjunctiva for the cysts and the following results were obtained. The survey showed that 285 cattle, representing 6 per cent of a total of 4,725 cattle examined had S.C. cysts. Only 49 (14 per cent) of them showed clinical sclerodermatitis and the ratio between clinical and inapparent cases was 1:5.8. On age distribution of the S.C. positive cases the highest incidence(10-12 per cent) was seen in amimals that were from 5 to 8 years of age, but clinically apparent cases occurred much more in younger age of animal. In local incidence of S.C. cysts positive cases the prevalence was higher in the secluded districts such as Koheung and Sancheoung counties(9 per cent).
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Studies of the water analysis of the breeding place of the first intermediate host(Semisulcospira libertina) was carried out. The breeding place of Semisulcospira libertina in the Haenam area were found mainly in pebbly and sandy riverbed of the upper stream and halfway up the Donghae river, but we could not find the habitats of Semisulcospira libertina in the spring, paddyfield and tidal part of flow in which the content of Cl ion, Ca ion, total alkalinity, total hardness and pH were obviously higher than those of the breeding place. The range of physical and chemical characters of the Semisulcospira libertina breeding water were as follows. 0.05-0.7 m/s of optimal flowing velocity of fresh water, 20-50 cm in depth, 7.12+/-0.17 of optimal pH range 19.4+/-4.92(mg/l)in total alkalinity, 9.4+/-2.97(mg/l) of Ca ion, 9.8+/-2.3(mg/l) of Cl ion content, 115.1+/-34.2(mg/l) of total hardness, but there were no remarkable differences between the habitat water and the contrasts in their specific gravities. In the comparison of analytical result of the breeding water at Haenam, the content of Ca ion, Cl ion, total alkalinity, total hardness and pH showed a gradually higher value in the lower reaches of the river than those of the upper stream and halfway up the river.
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In order to find out a valuable control measure for soil-transmitted parasties, the infectivity in mice of Ascaris eggs irradiated with Cobalt(60) were examined. The results were summarised as follows. In vitro, Ascaris eggs irradiated with larger doses of Cobalt(60) developed poorly, and no difference was found between fresh eggs and those cultured for a few days. Ascaris eggs irradiated with doses of 200,000 rad. developed at the rate of 90 percent after 4 weeks, whereas those irradiated with 1,000,000 rad.
developed 28 percent. Ascaris eggs irradiated with Cobalt(60) after 2 weeks of culture were poor in development compared with those of 4 week culture. Eggs cultured for 5 weeks showed weaker infectivity in mice than those cultured for 8 weeks. In the control groups, the infectivity in mice of Ascaris eggs was remained the same between 5 and 8 weeks.
The minimum dose of Cobalt(60) irradiation effective for preventing infectivity in mice was estimated to be 200,000 rad.
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