Anisakiasis (anisakidosis) refers to a foodborne zoonosis caused by ingesting raw or undercooked marine fish or cephalopods infected with anisakid larvae. The present study was performed to investigate the prevalence of anisakid larvae in anchovies (Engraulisjaponica) purchased from 2 local markets in Gyeongsangnam-do, the Republic of Korea (=Korea), during 2018-2019. Anchovies were transported to our laboratory and examined by pepsin-HCl artificial digestion technique followed by microscopic observations and molecular analyses. The overall prevalence of anisakid larvae was 19.5% (39/200), from which a total of 51 larvae (av. 1.3 larvae/infected anchovy) were recovered. Sequencing of the larvae targeting the ITS region, including ITS1, 5.8S rRNA, and ITS2 genes confirmed the species of larvae as Anisakispegreffii (54.9%; 28/51), Hysterothylaciumsinense (23.5%; 12/51), and Hysterothylaciumaduncum (21.5%; 11/51). The results suggested that anchovies could be a potential source of human anisakiasis in Korea.
Citations
Citations to this article as recorded by
Occurrence and molecular identification of anisakid nematodes in anchovies from the Gulf of California, Mexico Dania López-Moreno, Marta Valmaseda-Angulo, Jesús Servando Hernández-Orts, José A. Cruz-Barraza, Emigdio Marín-Enríquez, Rebeca Sánchez-Cárdenas, Marlenne Manzano-Sarabia, Felipe Amezcua, Juan R.F. Vallarta-Zarate, Francisco N. Morales-Serna Food Control.2026; 180: 111657. CrossRef
Genetic analyses of Anisakis pegreffii (Nematoda: Anisakidae) from the East Asian finless porpoise Neophocaena asiaeorientalis sunameri (Cetacea: Phocoenidae) in Korean waters Sunmin Kim, Jong Yoon Jeon, Kyunglee Lee, Hyunjoo Lee, Han Chan Park, Kyung Eun Lee, Hang Lee, Sung Bin Lee, Sang Wha Kim, Se Chang Park, Seongjun Choe, Heejeong Youn Parasitology Research.2024;[Epub] CrossRef
Diatoms-endoparasite association in fish from the marine pacific coast of Colombia (Buenaventura) Vanessa Potosi-Pai, Carlos E. Agudelo Morales, Javier Antonio Benavides-Montaño, Shawky M. Aboelhadid PLOS ONE.2024; 19(12): e0312015. CrossRef
Anisakis simplex sensu stricto (s.s.), Anisakis pegreffii, Anisakis berlandi (=A. simplex sp. C), and Anisakis typica are the 4 major species of Anisakis type I larvae. In the Republic of Korea (Korea), A. pegreffii, A. berlandi, and A. typica larvae in fish hosts has seldom been documented. In this study, molecular analysis was performed on Anisakis larvae from the sea eels (Astroconger myriaster), the major source of human anisakiasis in Korea, collected from Tongyeong City, a southern coastal area of Korea. All 20 sea eels examined were infected with Anisakis type I larvae (160 larvae; 8 per fish). Their species were analyzed using PCR-RFLP patterns and nucleotide sequences of internal transcribed spacers (ITS1, 5.8 subunit gene, and ITS2) and mitochondrial cytochrome c oxidase 2 (cox2). Most (86.8%; 112/129) of the Anisakis type I larvae were A. pegreffii, and 7.8% (10/129) were A. typica. The remaining 5.4% (7/129) was not identified. Thus, A. pegreffii is the major species of anisakid larvae in sea eels of the southern coast of Korea.
Citations
Citations to this article as recorded by
Anisakidae and Anisakidosis: A Public Health Perspective Diana Nonković, Vanja Tešić, Vida Šimat, Svjetlana Karabuva, Alan Medić, Jerko Hrabar Pathogens.2025; 14(3): 217. CrossRef
Rapid and Ultrasensitive Detection of H. aduncum via the RPA-CRISPR/Cas12a Platform Xiaoming Wang, Xiang Chen, Ting Xu, Xingsheng Jin, Junfang Jiang, Feng Guan Molecules.2024; 29(20): 4789. CrossRef
Genetic analyses of Anisakis pegreffii (Nematoda: Anisakidae) from the East Asian finless porpoise Neophocaena asiaeorientalis sunameri (Cetacea: Phocoenidae) in Korean waters Sunmin Kim, Jong Yoon Jeon, Kyunglee Lee, Hyunjoo Lee, Han Chan Park, Kyung Eun Lee, Hang Lee, Sung Bin Lee, Sang Wha Kim, Se Chang Park, Seongjun Choe, Heejeong Youn Parasitology Research.2024;[Epub] CrossRef
Prevalence of Anisakid Nematodes in Fish in China: A Systematic Review and Meta-Analysis Qing Liu, Qi Wang, Jing Jiang, Jun-Yang Ma, Xing-Quan Zhu, Qing-Long Gong Frontiers in Veterinary Science.2022;[Epub] CrossRef
Demographic history and population genetic structure of Anisakis pegreffii in the cutlassfish Trichiurus japonicus along the coast of mainland China and Taiwan Fang Ding, Sui Gu, Mu-Rong Yi, Yun-Rong Yan, Wei-Kuang Wang, Kwong-Chung Tung Parasitology Research.2022; 121(10): 2803. CrossRef
First morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from marine fishes in Vietnamese water Hoang Van Hien, Bui Thi Dung, Ha Duy Ngo, Pham Ngoc Doanh Journal of Nematology.2021; 53(1): 1. CrossRef
Comparison of risk factors between human intestinal and gastric Anisakiasis Kazuki Yamamoto, Osamu Takahashi, Daiki Kobayashi Parasitology International.2020; 75: 102024. CrossRef
Genera and Species of the Anisakidae Family and Their Geographical Distribution Juan C. Ángeles-Hernández, Fabian R. Gómez-de Anda, Nydia E. Reyes-Rodríguez, Vicente Vega-Sánchez, Patricia B. García-Reyna, Rafael G. Campos-Montiel, Norma L. Calderón-Apodaca, Celene Salgado-Miranda, Andrea P. Zepeda-Velázquez Animals.2020; 10(12): 2374. CrossRef
Establishment and validation of ARMS (amplification-refractory mutation system) for identification of Anisakis species collected from Korean waters Hyunsu Kim, Kyung-Wan Baek, Mi-Kyung Park, Kyung-Yoon Jeon, Eun-Ji Ko, Hee-Jae Cha, Mee Sun Ock Gene.2019; 691: 125. CrossRef
Molecular Identification of Anisakis Larvae Extracted by Gastrointestinal Endoscopy from Health Check-up Patients in Korea Hyemi Song, Bong-Kwang Jung, Jaeeun Cho, Taehee Chang, Sun Huh, Jong-Yil Chai The Korean Journal of Parasitology.2019; 57(2): 207. CrossRef
Anisakid Larvae from Anchovies in the South Coast of Korea Taehee Chang, Bong-Kwang Jung, Sooji Hong, Hyejoo Shin, Jeonggyu Lee, Laddawan Patarwut, Jong-Yil Chai The Korean Journal of Parasitology.2019; 57(6): 699. CrossRef
Anisakiasis Causing Acute Dysentery in Malaysia Amirah Amir, Romano Ngui, Yee-Ling Lau, Wan Hafiz Wan Ismail, Rohela Mahmud, Kum T. Wong, Jaxinthe S. K. Ong, Yvonne A. L. Lim The American Journal of Tropical Medicine and Hygiene.2016; 95(2): 410. CrossRef
First
Prev
