Parasites, Hosts and Diseases

"Bae Keun Park"

Brief Communications

Alaria alata (Digenea: Diplostomidae) from Korean Raccoon Dog, Nyctereutes procyonoides koreensis, in Korea: Heon Woo Lee, Eui Ju Hong, Hyeon Cheol Kim, Si Yun Ryu, Bae Keun Park; Korean J Parasitol 2022;60(5):361-365.
Published online October 21, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.5.361

Abstract
PDF

We report a species of diplostomid fluke recovered from 3 carcasses of wild Korean raccoon dog, Nyctereutes procyonoides koreensis, in Korea. A total of 107 diplostomid flukes were recovered from the small intestines of Korean raccoon dogs, which were obtained from the Gangwon Wildlife Medical Rescue Center. Worms fixed with 10% neutral formalin were subjected to microscopic observation and those fixed in 70% ethanol were used for molecular genomic analysis. The worm was divided into 2 separate parts, forebody and hindbody, with a total length of 3,020-4,090 (3,855) µm and a width of 1,210-1,770 (1,562) µm. The boat-shaped forebody has a pair of characteristic tentacular appendage, 2 suckers, holdfast organ, and vitelline follicles. The oval to cylindrical hindbody has reproductive organs. The ovary was round or elliptical and located in the anterior of the testes. Two large testes were slightly segmented and tandemly arranged, occupying almost half of hindbody. The short uterus contained a relatively small number of unembryonated eggs sized 130-140×85-96 µm. The partial sequence of 18S rRNA of this fluke was consistent with Alaria alata. Based on the morphological and molecular characteristics, the diplostomid flukes recovered from the small intestine of Korean raccoon dogs were identified as A. alata (Digenea: Diplostomidae).

Citations

Citations to this article as recorded by

Ultrasonographic evaluation of gastrointestinal wall thickness in Korean raccoon dogs (Nyctereutes procyonoides koreensis)
Yoonju Choi, Myeongsu Kim, Jae-Ik Han, Kichang Lee, Hakyoung Yoon
Journal of Veterinary Science.2025;[Epub] CrossRef

4,498 View
160 Download
1 Web of Science
Crossref

Cryptocotyle lata (Trematoda: Heterophyidae) Adult from a Korean Raccoon Dog, Nyctereutes procyonoides koreensis: Heon Woo Lee, Eui Ju Hong, Hyeon Cheol Kim, Si Yun Ryu, Bae Keun Park; Korean J Parasitol 2021;59(6):645-649.
Published online December 22, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.6.645

Abstract
PDF

Total 513 heterophyid flukes were collected from a carcass of wild Korean raccoon dog, Nyctereutes procyonoides koreensis, in Korea. With morphological and molecular characteristics, the flukes were identified to Cryptocotyle lata. The adult C. lata were minute, transparent, pentagonal, 522 µm long by 425 µm wide. Ceca extended into post-testicular region. Ventrogenital sac elliptical, 79 µm by 87 µm with genital pore and ventral sucker. Two testes semielliptical and slightly lobed, located in the posterior region, right testis 173 µm by 155 µm, left testis 130 µm by 134 µm. In a phylogenetic tree, the fluke specimen of this study was grouped with C. lata divergent from Cryptocotyle lingua. We report here N. procyonoides koreensis first as a natural definitive host of C. lata.

4,165 View
122 Download

Euryhelmis squamula (Digenea: Heterophyidae) Recovered from Korean Raccoon Dog, Nyctereutes procyonoides koreensis, in Korea: Hyeon Cheol Kim, Eui Ju Hong, Si Yun Ryu, Jinho Park, Jeong Gon Cho, Do Hyeon Yu, Joon Seok Chae, Kyoung Seong Choi, Bae Keun Park; Korean J Parasitol 2021;59(3):303-309.
Published online June 21, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.3.303

Abstract
PDF

In this study, we intended to describe an unrecorded species of heterophyid trematode recovered from the small intestine of a Korean raccoon dog, Nyctereutes procyonoides koreensis, in Korea. A total of 13 small flukes were collected from a deceased Korean raccoon dog which was found in Chuncheon-si, Gangwon-do, Korea in May 2017. The trematode body were covered with many small spines, rectangular, broader than long, 807-1,103 µm long and 1,270-1,550 µm wide. Oral sucker in the anterior end slightly smaller than acetabulum. Pharynx muscular and well developed. Esophagus relatively long and sigmoid. Acetabulum small and located at median in anterior 2/5 portion. Ceca bifurcated at the anterior of genital pore and acetabulum and terminated at testis level. Testes larger, deeply lobed and located at the near of posterior end of body. Ovary small, triangular and located at the slight left of median and the anterior of left testis. Vitelline follicles dendritic and extend from the middle level of esophagus to the posterior portion of body. Eggs embryonated, operculated, small and 33-35×15-16 µm in size. Based on the morphological characteristics, the small heterophyid flukes recovered from the small intestines of Korean raccoon dog, N. procyonoides koreensis, were identified as Euryhelmis squamula (Digenea: Heterophyidae). Accordingly, this species of heterophyid flukes is to be a new trematode fauna in Korea by this study.

Citations

Citations to this article as recorded by

Morbidity in California giant salamander (Dicamptodon ensatus Eschscholtz, 1833) caused by Euryhelmis sp. Poche, 1926 (Trematoda: Heterophyiidae)
Jaimie L. Miller, Lawrence Erickson, Susanne Fork, Constance L. Roderick, Daniel A. Grear, Rebecca A. Cole
International Journal for Parasitology: Parasites and Wildlife.2024; 23: 100908. CrossRef
The grey wolf (Canis lupus) as a host of Echinococcus multilocularis, E. granulosus s.l. and other helminths – a new zoonotic threat in Poland
Jacek Karamon, Małgorzata Samorek-Pieróg, Ewa Bilska-Zając, Weronika Korpysa-Dzirba, Jacek Sroka, Jolanta Zdybel, Tomasz Cencek
Journal of Veterinary Research.2024; 68(4): 539. CrossRef
Cryptocotyle lata (Trematoda: Heterophyidae) Adult from a Korean Raccoon Dog, Nyctereutes procyonoides koreensis
Heon Woo Lee, Eui Ju Hong, Hyeon Cheol Kim, Si Yun Ryu, Bae Keun Park
The Korean Journal of Parasitology.2021; 59(6): 645. CrossRef

4,693 View
84 Download
3 Web of Science
Crossref

Original Articles

Morphological and Molecular Characterization of Toxocara apodemi (Nematoda: Ascarididae) from Striped Field Mice, Apodemus agrarius, in Korea: Hyeon Cheol Kim, Eui Ju Hong, Si Yun Ryu, Jinho Park, Jeong Gon Cho, Do Hyeon Yu, Joon Seok Chae, Kyoung Seong Choi, Bae Keun Park; Korean J Parasitol 2020;58(4):403-411.
Published online August 25, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.4.403

Abstract
PDF

Adult ascarid worms from the field mice, Apodemus agrarius, were observed with a light and scanning electron microscope, and molecularly analized with 18S rRNA gene. In the scanning electron microscope, 3 prominent labia were present in the anterior end of male and female worms, but the interlabia and gubernaculum were absent. Scanning electron micrographs showed cervical alae as vestigial organs that looked like a slightly uplifted superficial sewing stitch. Total 6 pairs of post-cloacal papillae were observed on the tail of the male worms. The tail of female worms was blunt and conical shape with a spine-like structure, mucron. The eggs were sub-globular, coated with the albuminous layer and 73 by 82 μm in average size. The superficial pits of T. apodemi egg (mean 8.6×6.7 μm) are obviously bigger than those of Toxocara spp. The partial sequence of 18S rRNA showed the sequence homology of Toxocara canis (99.6%), Toxocara cati (99.4%), Toxascaris leonina (99.4%), and Toxocara vitulorum (99.2%). Conclusively, it was confirmed that ascarid nematodes, Toxocara apodemi, recovered from striped field mice in Korea are taxonomically conspecific relationship with genus Toxocara and genetic divergence from other Toxocara species.

Citations

Citations to this article as recorded by

First molecular characterization and species validation of Toxocara alienata (Rudolphi 1819) (Nematoda: Ascarididae), from a crab-eating raccoon (Procyon cancrivorus)
Gustavo Macêdo do Carmo, Lorena Gisela Ailán-Choke, Hassan Jerdy, Hermínio Ismael de Araújo-Júnior, Felipe Bisaggio Pereira
Parasitology International.2026; 111: 103160. CrossRef
Parasites in the Digestive Tract of Lion (Panthera leo) at the Safari Park of Gurun Putih Lestari Jantho, Aceh Besar
Fajri Dwitama, Razali Daud, Arman Sayuti, Yudha Fahrimal
Journal of Applied Veterinary Science And Technology.2025; 6(1): 32. CrossRef
Nematocidal activity of zinc oxide nanoparticles synthesized using chicken egg albumin in lung and spleen of Parascaris equorum experimentally infected rats
Sara Bayoumi Ali, Ayman Saber Mohamed, Sohair R. Fahmy, Manal El–Garhy, Mohamed R. Mousa, Fathy Abdel-Ghaffar
Scientific Reports.2025;[Epub] CrossRef
Incidence and Risk Factors of Toxocara vitulorum Infection in Beef Cattle of Yogyakarta, Indonesia
Vika Ichsania Ninditya, Fitrine Ekawasti, Joko Prastowo, Irkham Widiyono, Wisnu Nurcahyo
World's Veterinary Journal.2024; 14(4): 592. CrossRef
Characterisation of the mitochondrial genome and phylogenetic analysis of Toxocara apodemi (Nematoda: Ascarididae)
Y. Gao, Y. Hu, S. Xu, H. Liang, H. Lin, T. H. Yin, K. Zhao
Journal of Helminthology.2024;[Epub] CrossRef
The Complete Mitogenome of Toxocara vitulorum: Novel In-Sights into the Phylogenetics in Toxocaridae
Yue Xie, Lidan Wang, Yijun Chen, Zhao Wang, Pengchen Zhu, Zun Hu, Xinfeng Han, Zhisheng Wang, Xuan Zhou, Zhicai Zuo
Animals.2022; 12(24): 3546. CrossRef

9,311 View
201 Download
5 Web of Science
Crossref

Urogonimus turdi (Digenea: Leucochloridiidae) from the White’s Thrush, Zoothera aurea, in the Republic of Korea: Hyeon Cheol Kim, Eui Ju Hong, Si Yun Ryu, Jinho Park, Do Hyeon Yu, Joon Seok Chae, Kyoung Seong Choi, Cheolho Sim, Bae Keun Park; Korean J Parasitol 2019;57(5):461-467.
Published online October 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.5.461

Abstract
PDF

Avian trematodes, Urogonimus turdi (Digenea: Leucochloridiidae), were collected from the intestine of wild birds, Zoothera aurea, 2013-2017 in the Daejeon Metropolitan City, Korea. The body was ellipsoidal, attenuated and/or round ends, 1,987-2,120 long and 819-831 ?m wide. The oral sucker was subterminal, rounded anteriorly, and 308- 425×351-432 ?m in size; the prepharynx and esophagus were almost lacking; pharynx was well-developed, 142- 179×78-170 ?m in size; intestine narrow, bifurcating just after pharynx, ascending to the oral sucker before looping posteriorly and terminating near the posterior end; ventral sucker larger, in almost median, 536-673×447-605 ?m and approximately 1.5 times larger than oral sucker. A phylogenetic tree constructed with 18S ribosomal RNA showed inter- and intraspecific relationships. Based on these morphological and molecular findings, we report here a U. turdi from White’s thrushes in Korea.

Citations

Citations to this article as recorded by

Complete life cycle of Parorchis trophoni sp. nov. (Digenea: Philophthalmidae) from the Southwestern Atlantic coast, Argentina, revealed by morphological and molecular data
Julia Inés Diaz, Sofía Capasso, Carmen Gilardoni, Eliana Lorenti, Vasyl V. Tkach, Florencia Cremonte
Polar Biology.2023; 46(8): 737. CrossRef
Rediscovery of Michajlovia turdi (Digenea: Brachylaimoidea) from Japan
Mizuki Sasaki, Takashi Iwaki, Minoru Nakao
Journal of Parasitology.2022;[Epub] CrossRef
Molecular identification of the broodsacs from Leucochloridium passeri (Digenea: Leucochloridiidae) with a review of Leucochloridium species records in Taiwan
Ming-Chung Chiu, Zhao-Hui Lin, Po-Wei Hsu, Hsuan-Wien Chen
Parasitology International.2022; 91: 102644. CrossRef
Nematodes and trematodes associated with terrestrial gastropods in Nottingham, England
P. S. Andrus, R. Rae, C. M. Wade
Journal of Helminthology.2022;[Epub] CrossRef

8,176 View
141 Download
4 Web of Science
Crossref

Molecular Identification of Haemadipsa rjukjuana (Hirudiniformes: Haemadipsidae) in Gageo Island, Korea: Sohyun Won, Bae Keun Park, Baek Jun Kim, Hye Won Kim, Jun Gu Kang, Tae Seo Park, Hong Yul Seo, Ye Eun, Ki Gyoung Kim, Joon Seok Chae; Korean J Parasitol 2014;52(2):169-175.
Published online April 18, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.2.169

Abstract
PDF

There are 60 species of blood-feeding land leeches, 50 species belonging to the family Haemadipsidae and 10 species belonging to the family Xerobdellidae. Despite recent papers on the land leeches, their taxonomic identification is not fully understood, especially at a species level. In Korea, there have been no historical records of the terrestrial leeches, but recently an unrecorded blood-feeding land leech was discovered at Gageo-do (Island), Korea. Molecular analysis was used to identify the species of 29 leeches collected from Mt. Dock-Sil in Gageo-do. Conventional PCR was conducted using nuclear 18S rRNA and mitochondrial cytochrome c oxidase subunit 1 (CO1) genetic marker. The 18S rRNA sequences revealed that the leeches share 99.9% identity with Haemadipsa rjukjuana (inhabiting Taiwan), and the CO1 sequences revealed that the leeches are very close to H. rjukjuana (inhabiting Taiwan). The CO1 sequences were separated into 2 categories, 1 with 94.6% and the other with 94.3% similarity to the H. rjukjuana L00115A (inhabiting Taiwan). This new finding of the land leech is the first record in Korea. In addition, the north range of the distribution of the blood-feeding leech (Hirudiniformes: Haemadipisidae) should be reconsidered including Korea.

Citations

Citations to this article as recorded by

Unraveling the structure, chemical composition, and conserved signaling in leech teeth
Yam Prasad Aryal, Sanjiv Neupane, Hee-Jin Kwak, Chang-Hyeon An, Wern-Joo Sohn, Hitoshi Yamamoto, Tae-Yub Kwon, Bong-Ki Min, Jae-Young Kim, Sung-Jin Cho
Animal Cells and Systems.2024; 28(1): 272. CrossRef
Differential Analysis in DNA Molecular and Protein Composition of Hirudo Nipponia Whitman Using DNA Barcoding and Protein-Based Reversed-Phase Hplc Fingerprint Analysis
Qian Gao, Jianyuan Tang, Li Zhiyong, Hang Xiao, Zhaoshun Luo, Mengmeng Shi, Linchun Shi, Feng Qiu, Li Ma
SSRN Electronic Journal.2023;[Epub] CrossRef
First Record of a Cavernous Land Leech Sinospelaeobdella cavatuses (Hirudinda: Haemadipsidae) from Thailand
Teerapong Seesamut, Ratmanee Chanabun, Natdanai Likhitrakarn, Warut Siriwut, Ruttapon Srisonchai, Arthit Pholyotha, Chirasak Sutcharit, Ekgachai Jeratthitikul
Tropical Natural History.2023; (7): 213. CrossRef
Characterization of the complete mitochondrial genome of Haemadipsa tianmushana Song 1977 (Hirudiniformes, Haemadipsidae) and its phylogenetic analysis
Fuhua Lu, Mengmeng Shi, Jiali Liu, Weijun Kong, Yufeng Zhang, Linchun Shi
Mitochondrial DNA Part B.2022; 7(1): 103. CrossRef
An annotated checklist of the eukaryotic parasites of humans, exclusive of fungi and algae
Blaine A. Mathison, Sarah G. H. Sapp
ZooKeys.2021; 1069: 1. CrossRef
Nuclear microsatellite and mitochondrial DNA analyses reveal the regional genetic structure and phylogeographical history of a sanguivorous land leech, Haemadipsa japonica, in Japan
Kaori Morishima, Mineaki Aizawa
Ecology and Evolution.2019; 9(9): 5392. CrossRef
Analysis of genetic variation in mitochondrial cytochrome c oxidase subunit 1 between Haemadipsa japonica in Japan and land leeches worldwide
Naoe Sato, Chikako Yokoyama, Miki Inukai, Saeko Miyashita, Keito Nagase, Takafumi Nakano, Katsuya Iuchi, Hisashi Hisatomi
Mitochondrial DNA Part B.2019; 4(1): 1408. CrossRef
Ehrlichia species in pond-farmed leeches (Hirudinaria sp.) in Hubei Province, China
Shu-Han Zhou, Xiao Xiao, Yi-Na Sun, Xiao-Hui Xu, Xin Ding, Si-Yi Zhang, Min Zhang, Wen-Liang Lv, Qing-Hua Gao, J. Stephen Dumler
PLOS ONE.2019; 14(4): e0215082. CrossRef
Characterization of 13 polymorphic microsatellite loci in the Japanese land leech
Kaori Morishima, Tomohiro Suzuki, Mineaki Aizawa
Parasitology International.2018; 67(1): 13. CrossRef
Bloodlines: mammals, leeches, and conservation in southern Asia
Michael Tessler, Sarah R. Weiskopf, Lily Berniker, Rebecca Hersch, Kyle P. Mccarthy, Douglas W. Yu, Mark E. Siddall
Systematics and Biodiversity.2018; 16(5): 488. CrossRef
Development of a Novel Wound Dressing Coated with Drug-loaded Mesenchymal StemCells to Promote Wound Healing in Diabetics
Albandari Bin-ammar, Mark Slevin, Nessar Ahmed, Donghui Liu
ETP International Journal of Food Engineering.2018; : 245. CrossRef
Molecular detection of Bartonella spp. in terrestrial leeches (Haemadipsa rjukjuana) feeding on human and animal blood in Gageo-do, Republic of Korea
Jun-Gu Kang, Sohyun Won, Hye-Won Kim, Baek-Jun Kim, Bae-Keun Park, Tae-Seo Park, Hong-Yul Seo, Joon-Seok Chae
Parasites & Vectors.2016;[Epub] CrossRef